• Title/Summary/Keyword: purifying

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Separation and Identification of Antimicrobial Compounds from Korean Leek (Allium tuberosum) (부추의 항균활성 물질의 분리)

  • Hong, Jeong-Hwa;Lee, Mi-Hyung;Kang, Min-Chul;Hur, Sung-Ho
    • Journal of Food Hygiene and Safety
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    • v.15 no.3
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    • pp.235-240
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    • 2000
  • Various separation techniques of antimicrobial compounds from food materials have proven to be ineffective, involving liquid-liquid extraction and chromatography. We established efficient separation procedure for partially purifying antimicrobial compounds from Korean leek. Separation procedures consisted of ultrafiltration, liquid-liquid extraction, TLC and HPLC. Molecular weight of antimicrobial compounds were less than 5,000 dalton. Major compounds were propenyl methyl disulfide, S-methyl methylthiosulphonate and dimethyl disulfide.

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Purification of Multi Walled Carbon Nanotubes (Mwcnts) Synthesized by Arc Discharge Set Up

  • Malathi, Y.;Padya, Balaji;Prabhakar, K.V.P.;Jain, P.K.
    • Carbon letters
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    • v.11 no.3
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    • pp.184-191
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    • 2010
  • Carbon nanotubes are unique tubular structures of nanometer diameter and large length/diameter ratio. The nanotubes may consist of one up to tens and hundreds of concentric shells of carbons with adjacent shells separation of ~0.34 nm. Multiwalled carbon nanotubes were synthesized by arc-discharge technique. MWCNTs were formed at the cathode deposit along with other carbonaceous materials like amorphous carbon, graphite etc. However, to get the best advantage of carbon nanotubes in various advanced applications, these undesired carbonaceous materials to be removed which is a challenging task. In the present study, various techniques were tried out for purifying MWCNTs such as physical filtration, chemical treatment and thermal annealing. SEM, FTIR, TGA and BET techniques were used to characterize the CNTs at various stages. Results shows that suitable chemical treatment followed by thermal annealing under controlled flow of oxygen gives the better route for purification of carbon nanotubes.

Purification Ability of Indoor Plants for Volatile Organic Compounds (VOCs) (실내식물의 휘발성유기화합물질 정화에 관한 연구)

  • Park, Soyoung;Kim, Jeoung;Jang, Young-Kee;Sung, Kijune
    • Journal of Environmental Impact Assessment
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    • v.15 no.6
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    • pp.417-423
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    • 2006
  • The purification ability of indoor plants for volatile organic compounds was investigated. Philodendron selloum and Spathiphyllum sp. were tested for removal of toluene and trichloroethylene in the artificially contaminated reactor under laboratory conditions. Each plant was placed in right side of the reactor and the TCE and toluene concentration change with time were monitored. In the reactor with Philodendron, the TCE concentrations of left and right sides were compared to examine the removal effects by plant. In the reactor with Spathiphyllum, air was circulated before sampling, and thus average removal effects by plants on target VOC were observed. Both plants showed clear effects on removal of VOCs from contaminated indoor air. The removal efficiency of Philodendron and Spathiphyllum were similar and showed 30 - 46% and 31 - 47% of purification effects, respectively. The results of this study showed that air purification using plants is an effective means of reduction on indoor VOCs concentration level and reduce related health risk though, supplementary purifying aids or proper ventilation were also suggested.

Separating and purifying polysaccharide, polypeptide from sea cucumber and their functions (해삼으로부터 다당류와 폴리펩타이드의 분리, 정제 및 기능성)

  • Jin, Hai-zhu;Fu, Xue-jun;Shen, Jing-yu;Sun, Bo;Wang, Hong-tao
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2005.09a
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    • pp.112-123
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    • 2005
  • For thestudying and utilizing the thorn sea cucumber synthetically, the method preparing polysaccharide and polypeptide from sea cucumber was studied. The comparatively rational craft for preparing crude polysaccharide is fresh raw materials deal with oar form, $60\%$ of the ethanol precipitate after hydrolyzed by pretense and vibrated with ultrasonic wave auxiliary. The purification of polysaccharide and removal of protein fromcrude polysaccharide were made through precipitation method using the acetate. The polypeptide is obtained by concentrating in vacuum, freeze-drying after mixing precipitations of two times. Polysaccharide acute poisoning experiment indicate there is no bad reaction when LD50>5000g/kg, there strain rate of liver tumor H11 approach present generally acknowledged tumor treat medicine cyclophosphamide, and there is no side effect. The fatigue resistance function of polypeptide experiment also indicates that the fatigue resistance ability of mouse which fed on added sea cucumber polypeptide has a great improvement.

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Nutritional Components and Safety of Purified Pufferfish (Lagocephalus gloveri) Liver Oil

  • Kim Dong-Soo
    • Fisheries and Aquatic Sciences
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    • v.6 no.4
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    • pp.172-179
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    • 2003
  • The safety of pufferfish (Lagocephalus gloveri) liver oil and the contents of some nutritional components were examined to obtain important information on their use as high valued functional foods. Pufferfish liver oil was extracted by the hot-water method using $1\%$ NaOH solution to remove toxic compounds, and then purified using a general purifying method of fish oil. Any extraordinary clinical symptoms were not observed from all groups administrated with pufferfish liver oil throughout the test period. None of the rats died when administrated the highest concentration of 10 mL/kg of the pufferfish liver oil. Vitamin A content was 114.2 ppm, as a retinal equivalent in the oil extracted using hot-water, but the content was higher (169.3 ppm) in oil extracted using n-hexane. Vitamin D and E were not detected in ppm in oil extracted using hot-water. Vitamin D in the pufferfish liver oil extracted using n-hexane was also undetected, but vitamin E was at 32.5 ppm. Among the 18 minerals detected, the sodium content was the highest at 253.5 ppm, followed by 13.9 ppm ofpotassium, 1.5 ppm of calcium, 0.2 ppm of magnesium, and other trace minerals. The contents of EPA and DHA were $0.8\%\;and\;14.8\%$, respectively, in the pufferfish liver oil extracted using hot-water. Considering these results, there is potential that pufferfish liver oil could be used as a functional food.

Plant Cultivation System Using the IoT (사물 인터넷을 이용한 식물재배시스템)

  • Lee, Gi-Young;Kim, Ho-Hyen;Jeong, Youn-Ha;No, Han-Hyul;Park, Yong-Wook
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.4
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    • pp.657-662
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    • 2017
  • For modern people who lack the time, the plants became more interested as they gave emotional stability and proved the purifying function of plants from harmful substances such as fine dust. In this study, we established a standard vegetation cultivation system that can easily grow indoors without any special knowledge, based on criteria such as temperature, soil humidity, and illumination. And we implemented a system that provides simple information needed for plant management by using Bluetooth function and provides contents that can get know-how necessary for plant management.

A Study on the Void Ratio and Permeability Coefficient Properties of fiber Reinforced Porous Concrete (섬유보강 포러스 콘크리트의 공극률과 투수계수 특성에 관한 연구)

  • Kim, Jeong-Hwan;Cho, Gwang-Yoen;Lee, Jun;Park, Seung-Bum
    • Proceedings of the Korea Concrete Institute Conference
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    • 2000.10a
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    • pp.677-682
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    • 2000
  • Porous concrete is defined as d type of concrete for which the fine aggregate component the matrix is entirely omitted. Although it had been used as a building material in Europe for over 60 years, low strength and high void ratio limited its application in the past. In recent years, however high void ratio of concrete has been recognized again and can be used as an environmental conscious material, for example, parking lots, draining light-traffic-volume pavements and as sea water purifying material. The result of an experiment on the void ratio of fiber reinforced porous concrete and its influence on the compressive strength and permeability relationship of concrete are reported in this paper. One-sized coarse aggregate of 5-10mm, and three absolute content of fiber(steel fiber, polyprophylen fiber) were used. The result of measured void ratio, permeability coefficient and compressive strength show a small variation. Void ratio, permeability coefficient and compressive strength of fiber reinforced porous concrete depend on contents of fiber and absolute volume ratios of paste to aggregate.

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Identification and Characterization of Homoharringtonine from Cephalotaxus koreana

  • Kim, Byung-Sik;Kim, Jin-Hyun
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.566-569
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    • 2005
  • A novel purification method was developed for producing homoharringtonine from Cephalotaxus koreana, to guarantee high purity and yield. Our simple, efficient procedure for isolating and purifying homoharringtonine from C. koreanabiomass consisted of solvent extraction, synthetic adsorbent treatment, low-pressure chromatography, followed by high performance liquid chromatography (HPLC). The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and dramatically increased the yield and purity of crude homoharringtonine for high-performance liquid chromatography (HPLC) purification steps compared with alternative processes. Homoharringtonine could be obtained simply with high yield and purity from biomass using this purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification. Purified homoharringtonine was identified and characterized.

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Size Distribution Characteristics and Entrapment Efficiency of Dried-Reconstituted Liposomes Containing Epidermal Growth Factor (상피세포성장인자를 함유한 동결건조-재분산 리포좀의 입도분포 및 봉입률)

  • Kim, Hee-Jun;Yu, Sung-Un;Choi, Young-Wook
    • YAKHAK HOEJI
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    • v.40 no.6
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    • pp.646-652
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    • 1996
  • Epidermal Growth Factor (EGF), discovered by Stanley Cohen in 1960, has a potential healing effect for wounds and bums. Considering wound care, in order to avoid physical stress at the wound surface and efficiently apply EGF, the need for viscous spraying solutions was essential. Viscous spraying solutions containing EGF were prepared by utilizing viscosity-building polymer, poloxamer 407, and by introducing liposome systems. On the other hand, EGF is purified on reverse HPLC gradient program with the mobile solvent of acetonitrile. It is necessary to observe liposomal EGF changes as the acetonitrile contents varied in order to introduce liposome systems at the step of EGF solution (at the time of EGF purifying). By evaluating the size distribution and entrapment efficiency of EGF liposome, it was possible to detemine the limit contents of acetonitrile and establish the optimal conditions for solution formulations. It has been revealed that, as the acetonitrile content increases, mean diameter of EGF liposomes increased and the width of size distribution tends to decrease. The limit contents of acetonitrile were 10%, since there was little difference to the acetonitrile free liposomes.

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The Search for Naturally Occurring Herbicidal Compounds - II. Isolation of Pinoresinol from Rhathiolepis ovata Briat and Its Biological Activity (천연에서부터 제초활성물질의 탐색 - 제2보 둥근잎가정큰나무(Rhathiolepis ovata Briat)로 부터 Pinoresinal의 단리 및 생물활성)

  • Ahn, J.W.;Choi, J.S.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.9 no.1
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    • pp.76-79
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    • 1989
  • As a result of screening search for biologically active substances to weed seeds among higher plants, MeOH extract from Rhathiolepis ovata Briat was found to inhibit germination of test weeds considerably. In the course of purifying the active substances, pinoresinol which showed very similar behavior with the active fraction on various chromatographies, was isolated from the same source, spectrally identified and bioassayed. Pinoresinol exhibited germination inhibitory activity against the common purslane (Portulaca oleracea L.) only ; the inhibitory effect was about 55% at concentration of 5 mg/ml.

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