• JSTS:Journal of Semiconductor Technology and Science
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• v.1 no.4
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• pp.232-238
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• 2001

A new DNA purification chip is proposed and fabricated for the sample preparation of Nucleic Acid (NA) probe assay. The proposed DNA purification chip is fabricated using photosensitive glass substrate and polydimethylsiloxane (PDMS) cover fixture. We have successfully captured and eluted the DNA using the fabricated photosensitive glass chip. The fabricated DNA purification chip showed a binding capacity of $15ng/\textrm{cm}^2$and a minimum extractable input concentration of $100copies/200\muL$. The proposed DNA purification chip can be applied for low-cost, disposable sample preparation of NA probe assays.

• Journal of the Korean Wood Science and Technology
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• v.30 no.3
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• pp.12-17
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• 2002

The extracellular invertase (EC 3.2.1.26) (Candida utilis) preparation was obtained from the liquid medium after desalting and freeze drying. This prepared enzyme was used for the comparative purification on 4 activated matrices by liquid column affinity chromatography method. In this method there were used controlled porous glass (CPG) silanized covalently activated by keratin, silanized silica gel and silica gel covalently covered by keratin. It was found that the invertase purification process was better using both CPG matrices (silanized CPG and keratin activated CPG) than these with two silica gel supports. Also the elution coefficient of the invertase from the two CPG columns was about 93 to 94%. Two silica gel supports found to be superior in terms of purification efficiency. The invertase purification process was confirmed by PAGE electrophoresis.

• Journal of the Korean Society of Radiology
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• v.14 no.7
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• pp.881-889
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• 2020

The objective of this study was to quantitatively measure the changes in radon concentration due to ventilation and air purification plants in the lower and upper floors of a building. This study measured and compared radon concentration in the lower and upper floors of the building by using a radon meter when the room was closed, it was ventilated, and air purification plants were installed at a specific time. One-way ANOVA was conducted to evaluate the effect of treatment (i.e., closure, ventilation, and air purification plants) on radon concentration. The results of this study showed that ventilation and air purification plants significantly decreased radon concentration in the lower and upper floors of the building, but the effect of ventilation and that of air purification plants were not significantly different. Therefore, it will be possible to reduce radon concentration effectively when ventilation and air purification plants are used appropriately.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.386-392
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• 1999

A large-scale culture of hepatitis A virus in human diploid MRC-5 cells was conducted. In a roller bottle culture, the virus was grown to a maximum titer in 3 weeks after infection. Over 95％ of the cell-associated virus was excreted after culturing the infected cells in suspension media without fetal bovine serum for 3 days. The cultured virus was inactivated with formalin, concentrated by ultrafiltration, and partially purified by ultracentrifugation in a non-ionic gradient medium of Renocal. Two separate peak fractions showing high anti-HAY ELISA titer were pooled and about 40％ of HAV antigen was recovered by this purification procedure. Of the partially purified vaccine, the protein pattern in SDS-PAGE and immunogenicity in mice were compared with a commercial HAV vaccine. In SDS-PAGE, the purified vaccine in this study and the commercial vaccine showed almost the same protein pattern. The seroconversion rate of the purified vaccine in mice was not different from that of the commercial vaccine. Therefore, we could prepare a good grade of HAV vaccine by a simple purification procedure although the purification itself was not completed.

• Journal of the Korean Wood Science and Technology
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• v.15 no.4
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• pp.12-17
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• 1987

To extract arabinogalactan from larch (Larix Leptolepis), chips immersed with water(20-$90^{\circ}C$) for 1hr, were defiberized by refiner. The liquors were recovered and purified to pure arabinogalactan by ion exchange resin(IRC-50 a. IR-45) or MgO. Additionally the optimal condition in purification with MgO was also investigated. 1. The amounts of solids(crude sugars) and pure arabinogalctan in solids are 8.6-11.3% and 7.3-8.5%(raw material = 100), respectively. 2. Phenolic materials in crude sugars are removed up to 96-89% by ion exchange resin and 94-88% by MgO, while recovery yields of pure arabinogalactan are 81-75% on purification with ion exchange resin and 91-87% on purification with MgO. 3. The optimal conditions of purification with MgO are the addition of 35mg MgO/0.5g of crude sugars, 45 minutes at $70^{\circ}C$, or 25 mg MgO, 30 minutes at $85^{\circ}C$.

• BMB Reports
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• v.43 no.5
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• pp.319-324
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• 2010

Experimental bioinformatics data obtained from an E. coli cell-based eukaryotic protein purification experiment were analyzed in order to identify any bottleneck as well as the factors affecting the target purification. All targets were expressed as His-tagged maltose-binding protein (MBP) fusion constructs and were initially purified by immobilized metal affinity chromatography (IMAC). The targets were subsequently separated from the His-tagged MBP through TEV protease cleavage followed by a second IMAC isolation. Of the 743 total purification trials, 342 yielded more than 3 mg of target proteins for structural studies. The major reason for failure of target purification was poor TEV proteolysis. The overall success rate for target purification decreased linearly as cysteine content or isoelectric point (pI) of the target increased. This pattern of pI versus overall success rate strongly suggests that pI should be incorporated into target scoring criteria with a threshold value.

• Journal of Environmental Science International
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• v.29 no.6
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• pp.623-631
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• 2020

This study analyzes the effect of negative air ions on the concentration of airborne particulate matter and evaluates the expected purification efficiency of open spaces for particulate matter by investigating the amount of negative air ions generated by plants. This study establishes a negative air ion generation treatment environment, plant environment, and control environment to measure the purification efficiency of particulate matter under the conditions of each, analyzing the expected purification efficiency by designing a particulate matter purification model. Results show that the amount of generated negative air ion according to environment was negative air ion generation treatment environment > plant environment > control environment; this order also applies to the particulate matter purification efficiency. Moreover, it took 65 min for the negative ion generation treatment environment, 90 min for the plant environment, and 240 min for the control environment to reach the standard expected purification efficiency of particulate matter concentration of 960 mg/㎥ for PM₁₀. For PM_2.5, with the designated maximum concentration of 700 mg/㎥, it took 60 min for the negative ion generation treatment environment, 80 min for the plant environment, and more than 240 min for the control environment. Based on these results, the expected purification efficiency compared to the control environment was quadrupled in the negative ion generation treatment environment and tripled in the plant environment on average.

• Journal of the Korean Wood Science and Technology
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• v.30 no.1
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• pp.34-39
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• 2002

This study examined the effect of carbonized wastewoods on purification of waste water. The purification ability of charcoals(lump-shaped, approximately 3×3×3 cm) maded by wood-based material for waste water from a kitchen and septic tank was superior to those of thinned wood. For lump-shaped charcoal, gaps between particles in particleboard, and gaps between fibers in MDF were much more effective than micropore in purification of waste water. After purification test, color of waste water from wood-based material charcoals were much more lighter than thinned wood charcoals. In addition, odors of waste water from both charcoals tended to be decreased.

• Korean Journal of Materials Research
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• v.26 no.6
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• pp.306-310
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• 2016

To produce 4N grade high-purity silica powder from natural ore, the mineralogical characteristics of natural silica ore were investigated and their effects on the purification process were revealed. The Chinese silica mineral ore used was composed of iron and aluminum as main impurities and calcium, magnesium, potassium, sodium, and titanium as trace impurities; these trace impurities generally exist as either single oxides or complex oxides. It was confirmed that liberation and acidic washing of the impurities were highly dependent on the particle size of the ground silica ore and on its mineralogical characteristics such as the distribution and phases of existing impurities. It is suggested that appropriate size reduction of silica ore should be realized for optimized purification according to the origin of the natural silica ore. A single step purification process, the mechano-chemical washing (MCW) process, was proposed and verified in comparison with the conventional multi step washing process.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.1
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• pp.243-248
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• 2014

This study was carried out to evaluate the economic value of development of separation and purification techniques of flavonoid in citrus using IRR, NPV. The separation and purification techniques of flavonoid of citrus was developed by national institute of horticultural and herbal science in Korea rural development administration. This technology could be used to improve efficiency and practicality of cultivation of citrus. The research results show that the technological value of separation and purification techniques of flavonoid of citrus was evaluated 3,155(scenario 1)~6,518(scenario 2) million won. IRR was 28.3%~61.2%, which was greater than discount rate(11.9%). NPV is 5,541~18,773 million won. Therefore, the economic validity of development of separation and purification techniques of flavonoid of citrus is identified by the results of technology evaluation.