• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1043-1052
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• 2019

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with $p-NPC_{16}$ at a pH of 8.5 and $50^{\circ}C$, and the $K_m$, $k_{cat}$, and $k_{cat}/K_m$ values were 1.05 mM, $292.95s^{-1}$ and $279s^{-1}mM^{-1}$, respectively. The lipase was highly stable at $7.5{\leq}pH{\leq}10.0$. $K^+$ and $Na^+$ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

• Asian-Australasian Journal of Animal Sciences
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• 제23권4호
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• pp.430-436
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• 2010

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제7권2호
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• pp.213-223
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• 1996

본 논문은 주의력결핍 과잉운동장애 아동을 대상으로 하여 이들의 우울, 불안증상을 평가하고, 이에 기초를 두어 현상론적인 측면에서 주의력결핍 과잉운동장애를 다시 세분화하는 것을 목적으로 하였다. 이를 위해 주의력결핍 과잉운동장애의 증상을 보이는 $9{\sim}12$세 까지의 환아 51명과 정상대조군 50명을 연구대상으로 하여, 한국형 소아우울척도와 소아의 상태-특성 불안척도를 사용하여 우울과 불안을 평가하였다. 주의력결핍 과잉운동장애 아동군은 한국형 소아우울척도상에서 대조군에 비하여 의미있게 높았으며 (p<0.01), 소아의 상태 불안척도상에서도 주의력결핍 과잉운동장애군이 대조군에 비하여 의미있게 높았다(p<0.05). 또한 각각의 척도상에서 기준점 이상의 점수를 획득하여 진단적으로 의미있는 대상아동이 각각이 존재하였다. 이상의 결과로 미루어 주의력결핍 과잉운동장애군을 현상학적으로는 순수한 주의력결핍 과잉운동장애, 우울형 주의력결핍 과잉운동장애, 불안형 주의력결핍 과잉운동장애로의 세분이 가능하였다. 따라서 이들 장애들도 새로운 아형으로 독립이 되어야 할 것이다.

• 한국결정성장학회지
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• 제25권4호
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• pp.166-171
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• 2015

본 연구에서는 고상법 및 flux를 이용하여 $Y_3Al_5O_{12}:Er^{3+}\;(YAG:Er^{3+})$ 분말을 저온에서 성공적으로 합성하였다. 분말의 합성 여부와 분말 하소 시 온도에 따른 결정성을 분석하기 위하여 X-ray diffraction(XRD)를 측정하였다. 순수한 YAG는 일반적인 고상법으로 합성할 경우, $1400^{\circ}C$에서 12시간 동안 하소하여 순수한 YAG 상을 얻을 수 있었고, 반면에 $BaF_2$를 첨가한 결과는 상대적으로 낮은 온도($1000^{\circ}C$)에서 합성되었다. 즉, 합성온도를 약 $400^{\circ}C$ 가량 낮출 수 있는 것으로 나타났다. 또한, $BaF_2$의 최적의 농도를 찾아 첨가 후, 열처리 온도에 따라 $BaF_2$로 인한 입자의 형태 및 크기를 조사하였으며 그에 따른 발광강도에 대하여 논의하였다.

• 폴리머
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• 제36권6호
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• pp.822-830
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• 2012

본 연구에서는 실리카 나노입자를 우수한 내가수분해성을 가지는 dipodal 형태의 bis[3-(trimethoxysilyl)propyl]amine(BTMA) 실란 커플링제로 표면 개질한 후, 실리카에 도입된 BTMA의 2차 아미노기인 N-H기와 마이클 부가반응이 가능한 acrylate기를 1분자당 2개씩 가지는 poly(ethylene glycol)diacrylate(PEGDA)로 표면 처리하여 acrylate기를 실리카 입자 표면에 도입하는 연구를 수행하였다. PEGDA의 몰수 및 3가지 서로 다른 분자량의 PEGDA(M.W. 258, 575, 700) 처리가 실리카 표면에 도입되는 acrylate기의 구조에 미치는 영향을 fourier transform infrared spectroscopy(FTIR), elemental analysis(EA, 원소분석) 및 고체 상태 $^{13}C$ cross-polarization magic angle spinning(CP/MAS) nuclear magnetic resonance spectroscopy(NMR)법을 사용하여 분석하였다. 액체상태의 순수 PEGDA와 순수 BTMA를 같은 몰 비로 반응시키면 PEGDA 1분자당 2개씩 존재하는 acrylate기 중 1개 acrylate기는 순수 BTMA의 N-H기와 1:1의 비율로 마이클 부가반응이 일어나 ${\beta}$-amino acid ester를 형성하고, 나머지 1개의 acrylate기는 남아서 중합반응이 가능한 단량체 합성이 가능하다. 하지만, BTMA로 개질된 실리카 입자를 PEGDA와 반응시키면, PEGDA 1분자당 2개씩 결합있는 acrylate기 대부분이 실리카 입자에 결합되어 있는 BTMA의 N-H기와 마이클 부가반응으로 ${\beta}$-amino acid ester를 형성하여, acrylate기의 C=C기 대부분이 C-C로 변화함을 확인하였다.

• Restorative Dentistry and Endodontics
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• 제27권6호
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• pp.632-643
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• 2002

The purpose of this study was two-fold. First was to evaluate whether the molecular sieving model was appropriate for ionic dissociation experiment. Second was to compare the dissociation of calcium and hydroxyl ions from five types of calcium hydroxide pastes (Pure calcium hydroxide paste, DT temporary $dressing^{\circledR},{\;}Metapaste^{\circledR},{\;}Chidopex^{\circledR},{\;}Metapex^{\circledR}$) in three vehicles (aqueous, viscous and oily) and the antibacterial effect. Each calcium hydroxide pastes was placed into 0.65ml tube with cap and then 15% polyacrylamide gel was placed onto calcium hydroxide pastes. After the gel was hardened, the tubes were filled with tridistilled water (pH 7.14) and closed with cap. The tubes were stored in $37^{\circ}C$ 100% incubator The pH reading and the concentration of calcium ions were taken at 1, 4, 7. 10, and 14 days. The brain heart infusion agar plates with S. mutans and A. actinomycetemcomitans were used far antibacterial activity test. Middle of agar plate was filled with the calcium hydroxide pastes. The plates were incubated at $37^{\circ}C$ and observations were made to detect the zones of inhibition. These data were evaluated statistically by use of the analysis of variance and duncan test. The results were as follows. 1. In fresh mixing state, the pH of five types of calcium hydroxide pastes were measured between 12.5 and 12.8. 2. The pH was increased in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (11.45) and DT temporary $dressing^{\circledR}$ (11.33) showed highest pH, followed by $Metapaste^{\circledR}$ (9.49), $Chidopex^{\circledR}$ (8.37) and $Metapex^{\circledR}$ (7.59) 3. Calcium was higher in all five types of calcium hydroxide pastes compared with control group. In 14 days, Pure calcium hydroxide paste (137.29 mg%) and DT temporary $dressing^{\circledR}$ (124.6 mg%) showed highest value, followed by $Metapaste^{\circledR}$ (116.74 mg%), $Chidopex^{\circledR}$ (111.84 mg%) and $Metapex^{\circledR}$ (60.22 mg%). 4. The zones of bacterial inhibition were seen around all five types of calcium hydroxide pastes. $Chidopex^{\circledR}{\;}and{\;}Metapex^{\circledR}$ groups which include iodoform were observed significantly larger zone of inhibition in A. actinomycetemcomitans compared with the other calcium hydroxide groups (p<0.05) However, $Metapex^{\circledR}$ showed the least antibacterial effect on S. mutans compared with other groups (p<0.05). The molecular sieving model was found to be acceptable in dissociation experiment of hydroxyl and calcium ions when compared with the previous tooth model study. But this model was not appropriate for the antibacterial test.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 추계학술발표대회
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• pp.41.1-41.1
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• 2009

Aerosol Deposition (AD) is anovel way to fabricate bioactive ceramic coatings in biomedical implants and prostheses applications. In the present work, silicon-substituted hydroxyapatite (HA) coatings on commercially pure titanium were prepared by aerosol deposition using Si-HA powders. The incorporation of silicon in the HA lattice is known to improve the bioactivity of the HA, makingsilicon-substitute HA an attractive alternative to pure HA in biomedical applications. Si-HA powders with the chemical formula $Ca_{10}(PO_4)_6-x(SiO_4)x(OH)_2-x$, having silicon contents up to x=0.5 (1.4 wt%), were synthesized by solid-state reaction of $Ca_2P_2O_7$, $CaCO_3$, and $SiO_2$. The Si-HA powders were characterized by X-ray diffraction (XRD), X-ray fluorescence spectrometry (XRF), and Fourier transform infrared spectroscopy(FT-IR). The corresponding coatings were also analyzed by XRD, scanning electron microscopy (SEM), and electron probe microanalyzer (EPMA). The results revealed that a single-phase Si-HA was obtained without any secondary phases such as $\alpha$- or $\beta$-tricalcium phosphate (TCP) for both the powders and the coatings.The Si-HA coating was about $5\;{\mu}m$ thick, had a densemicrostructure with no cracks or pores. In addition, the proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 preosteoblast cells grown on the Si-HA coatings were significantly higher than those on the bare Ti and pure HA coating. These results revealed the stimulatory effects induced by siliconsubstitution on the cellular response to the HA coating.

• 한국지반공학회지:지반
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• 제12권5호
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• pp.17-26
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• 1996

본 연구에서는 아산만 지역의 해안 연약층에서 채취한 해성토의 거동을 자동화된 삼축실험기를 사용하여 비배수 상태하에서 실내실험과 모델예측을 수행하여 파괴전과 파괴상태시 응력-변형률 상태를 점토질 및 실트질 흙과 비교분석하였다. 채취한 해성토는 실트질 70%와 점토질 30%가 섞인 혼합토로 현장의 비교란 시료와 이를 재 성형한 시료의 2종류로 만들어 400kpa인 유효구속압력까지 등방압밀 시킨 후 압밀하중을 감소시키며 구속압이 각각 400, 200, 100, 67kpa인 경우에 비배수 상태로 삼축압축 및 인장실험을 하였다. 본 연구결과 모든 시료의 극한상태를 연결하면 일정한 파괴선에 도달하였으며 이때 파괴선은 순수점토나 실트에 비해서는 그 기울기에 차이가 있었다. 또한 정규압밀된 아산만 해성토에서는 전단초기에는 순수점토와 유사하게 전단하에서는 양의 간극수압이 발생하여 P'이 계속 감소하나 실트질에서 나타나는 상태변형선을 지나서는 체적팽창경향이 나타나며 전단강도가 증가하고 있다. 과압밀 시료는 체적변형 경향으로 순수실트와는 상당히 다른 거동을 보임을 알 수 있었다. 수정 Camflay모델 및 항복경계면 모델을 사용하여 예측한 결과 정규압밀된 경우에는 최대강도 이전까지는 실제거동을 적절히 예측할 수 있었으나 과압밀비$(2\leqOCR\leq6)$가 커질수록 그 거동에는 정량적인 차이를 보임을 알 수 있었다.

• 한국재료학회지
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• 제23권4호
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• pp.240-245
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• 2013

Electrochemical surface treatment is commonly used to form a thin, rough, and porous oxidation layer on the surface of titanium. The purpose of this study was to investigate the formation of nanotubular titanium oxide arrays during short anodization processing. The specimen used in this study was 99.9% pure cp-Ti (ASTM Grade II) in the form of a disc with diameter of 15 mm and a thickness of 1 mm. A DC power supplier was used with the anodizing apparatus, and the titanium specimen and the platinum plate ($3mm{\times}4mm{\times}0.1mm$) were connected to an anode and cathode, respectively. The progressive formation of $TiO_2$ nanotubes was observed with FE-SEM (Field Emission Scanning Electron Microscopy). Highly ordered $TiO_2$ nanotubes were formed at a potential of 20 V in a solution of 1M $H_3PO_4$ + 1.5 wt.% HF for 10 minutes, corresponding with steady state processing. The diameters and the closed ends of $TiO_2$ nanotubes measured at a value of 50 cumulative percent were 100 nm and 120 nm, respectively. The $TiO_2$ nanotubes had lengths of 500 nm. As the anodization processing reached 10 minutes, the frequency distribution for the diameters and the closed ends of the $TiO_2$ nanotubes was gradually reduced. Short anodization processing for $TiO_2$ nanotubes of within 10 minutes was established.

• 무역상무연구
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• 제37권
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• pp.187-216
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• 2008

Risk regarding the possibility of loss can be especially problematic. If a loss is certain to occur, it may be planned for in advance and treated as a definite, known expense. It is when there is uncertainty about the occurrence of a loss that risk becomes an important problem. The word risk is often used in connection with insurance. No one generally accepted definition of risk exists, however. Of the many definitions, two distinctive ones are commonly used. One defines risk as the variation in possible outcomes of an event based on chance. That is, the greater the number of different outcomes that may occur, the greater the risk. Another way of expressing this concept is to state: The greater the variation around an average expected loss, the greater the risk. The second definition of risk is the uncertainty concerning a possible loss. The definition of risk as a useful one because it focuses attention on the degree of risk in given situations. The degree of risk is a measure of the accuracy with which the outcome of an event based on chance can be predicted. For now, it will serve our purpose to note the more accurate the prediction of the outcome of an event based on chance, the lower the degree of risk. After sources of risks are identified and measured, a decision can be made as to how the risk should be handled. A pure risk that is not identified does not disappear, the business merely loses the opportunity to consciously decide on the best technique for dealing with that risk. The process used to systematically manage risk exposures is known as risk management. Some persons use the term risk management only in connection with businesses, and often the term refers only to the management of pure risks. In this sense, the traditional risk management goal has been to minimize the cost of pure risk to the company. But as firms broaden the ways that they view and manage many different types of risk, the need for new terminology has become apparent. The terms integrated risk management and enterprise risk management reflect the intent to manage all forms of risk, regardless of type. International trade transaction is called between countries has features of globalism, cultural gap, long distance and long terms for the transaction. It is riskier than domestic transaction has its specific risks, such as foreign exchange risk and political risk, and requires various active risk management skills. Risks in relation to the international trade transaction are the contract risk, transit risk and payment risk, etc. The risk management in relation to the international trade transaction is to identify and measure these risks. The purpose of this study is to analyse the practical problems and its solution plan by analyzing various cases related to the risk management of bill of lading in the international trade transaction.