• Current Research on Agriculture and Life Sciences
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• v.20
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• pp.55-63
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• 2002

This experiment was to know properties of Sericin Jam that development, growth of silkgland, content of sericin and composition of amino acid in hemolymph. The characters of Sericin Jam can he seen form this experiment. Hatching ratio was 85% in Sericin Jam and 95% in Jam 120. Especially hatching period of Sericin Jam was longer than Jam 120 and also hatching of Sericin Jam was ununiform. The larval duration of Sericin Jam was 20 days and 23 hours, and Jaw 120 was 21 days and 22 hours. In Sericin Jam, middle silkgland contain a great p arts in silkgalod and posterior silkgand is short and no curves. The period of mounting to emergence was 12 days in Sericin Jam. The period of pupa was 7day. It is property of Sericin Jam that the period is short. Cocoon was very thin and light in Sericin Jam. Weight of cocoon shell of Sericin Jam is 2.7cg. The sericin protein quantity was 100% in cocoon shell of Sericin Jam, about 28% in cocoon shell of Jam 120, however the sericin ratio per Sericin Jam cocoon was 34.6% compare to cocoon shell of Jaw 120 in sericin protein quantity. The amino acids in hemolymph of Sericin Jaw was much hidtidine, lysine, glut amic acid. And the amount of almost amino acids were increased depends upon development at t he 5th instar.

• Journal of Sericultural and Entomological Science
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• v.20 no.2
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• pp.15-19
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• 1978

In order to identify hormone activities which related to the changing of cutaneous tissue and pupal bodies and also to identify the interaction of corpora allata hormone and prothoracic glandular band hormone which act upon the metamorphosis of the insect. The item of treatment is carried out in 5 different times at the interval of about 12 hours after 4 weeks from the first feeding of 4th instar stage, and as far as 5th instar stage in concerned 6 times of item treatments have been carried out, at about 12 hours interval after 72 hours from the molting, Item treatments were, made in the stage of spring, autumn rearing, However, during the period of post mounting stage and pre pupation by picking out of corpora allata in 5 times at the interval of hours the following change of pupal colour is observed; 1) By an early pick out of 4th instar stage, the three molters are found in greater number, However, by a later pick out the four molters are found instead of others. 2) An abnormal colour is found in the three and four molters when comparing with the control molters. 3) The most of control molters have shown the normal colour except a few samples. 4) As shown in the table 1 and 2, the death number of item treatment is greater in the autumn rearing season. 5) The picking out of corpora allata of 5th instar stage, the post mounting stage and pre pupation period is affecting the change of colour. 6) As a final conclusion, the corpora allata hormone is closely related to the changing of pupal colour.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.126-132
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• 2012

Two different feed(bran and swine feed) effect was tested on development of Tenebrio molitor at $25{\pm}5^{\circ}C$, $65{\pm}10%RH$. There was no significant feed effect on larval developmental periods(80.0 ~ 83.7 days), pupal length (18.1 ~ 18.7 mm) and fecundity(44.3 ~ 55.3 eggs) during first two days. However, fecundity was significantly affected by the pupal weight(P < 0.05), such that the number of eggs 172.3, 191.2, 215.0 gradually increased as pupal weight rose from 0.12 g, 0.15 g to 0.18 g, respectively. During the first two weeks of oviposition was 51.8%, which gradually decreased for further days. Longevity was $27.1{\pm}6.9$, $30.1{\pm}7.8$, $27.4{\pm}8.5$ days with increasing pupal weight of 0.12, 0.15, 0.18 g, respectively. Larval densities of 100, 300, 500 and 700 individuals reared in the container ($16{\times}21.5{\times}7cm$) resulted 67.9% of the T. molitor pupation during first three weeks. Pupal weight was decreased $146.9{\pm}6.5$, $142.1{\pm}8.3$, $122.0{\pm}9.6$, $118.5{\pm}5.2mg$ when it was reared at different larval densities of 100, 300, 500 and 700 individuals, respectively in size of($16{\times}21.5{\times}7cm$) rearing container.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.175-182
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• 2019

The citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillaridae), is an important pest that decreases the productivity of citrus trees by attacking citrus leaves, and especially it causes a severe damage during the young growth stage. In this study, we investigated the distribution of eggs on the leaves of shoots according to the position and the survival rate of this pest in the field condition. The occurrence patterns of P. citrella eggs in citrus orchards were largely affected by the occurrence of shoots. Even if the adults were still being attracted in the pheromone traps, the eggs were not observed on leaves when the growth of shoots was stopped. The density of P. citrella eggs in the citrus shoots peaked at the fifth or sixth leaf from the terminal leaf, and over 90% of the eggs were found in the first to eighth leaves as a whole. In 2015 and 2016, the average survival rate of larvae surviving to pupa was 1.4%, and the rate of survival to adults was 0.2%, indicating that most of them died. Furthermore, we have discussed the management strategy of P. citrella considering the distribution of eggs and the larval survival rate in the citrus shoots.

• Journal of Life Science
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• v.31 no.2
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• pp.219-222
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• 2021

In this study, we examined low molecular weight peptides using proteomics in order to identify their original proteins, derive their peptides, and determine the functions of the proteins in Tenebrio molitor, the mealworm (larvae, pupae, or adult) from which the peptides were extracted. Fifty-four proteins were finally identified through an analysis of proteome to derive the analyzed peptides. The proteins that induced low molecular weight peptides were identified to be the most abundant in adults only, and the next highest were derived from a group containing both adults and larva. However, other groups, including pupa, were detected to have a lower frequency of peptides. As a result of orthologous classification of the detected proteins, the general function prediction was only investigated at the highest frequency among the examined proteins. Proteins related to chromatin structure and dynamics were detected by their higher frequency among functional classes. The next highest frequency was shown by proteins related to amino acid transport and metabolism and carbohydrate transport and metabolism. Therefore, it is assumed that proteins correlated with chromatin, amino acid, and carbohydrate metabolisms are easily induced into low molecular weight peptides, and that their peptides could play a role as bioactive substances.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.563-575
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• 2022

Maruca vitrata is one of important pests in leguminous crops, especially red bean. We investigated the effects of temperature on development of each life stage, adult longevity and fecundity of M. vitrata for understanding the biological characteristics of the insect species at eight constant temperatures of 13, 16, 19, 22, 25, 28, 31, and 34℃. Eggs hatched successfully at all temperature subjected and larvae successfully developed to the adult stage from 16℃ to 31℃. The developmental period of egg decreased up to 31℃ and after then increased. The developmental period of larva and pupa, and adult longevity of M. vitrata decreased with increasing temperature. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of M. vitrata were estimated by linear regression as 12.8℃ and 280.8DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 14.2℃ and 31.9℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of M. vitrata was 17.7℃. In addition, we constructed the oviposition models of adult, using the investigated adult traits including survival, longevity, oviposition period and fecundity. Temperature-dependent development models and adult oviposition models will be helpful to understand the population dynamics of M vitrata and to establish the strategy of integrated pest management in legume crops.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.577-590
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• 2022

Ostrinia scapulalis is one of important pests in leguminous crops, especially red bean. In order to understand the biological characteristics of the insect, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of O. scapulalis at eleven constant temperatures of 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, and 36℃. Eggs and larvae successfully developed next life stage at most temperature subjected except 7, 10 and 13℃. The developmental period of egg, larva and pupa decreased as temperature increased. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of O. scapulalis were estimated by linear regression as 13.5℃ and 384.5DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 19.4℃ and 39.8℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of O. scapulalis was 20.4℃. Adults produced viable eggs at the temperature range between 16℃ and 34℃, and showed a maximum number, ca. 416 offsprings, at 25℃. Adult models including aging rate, age-specific survival rate, age-specific cumulative oviposition, and temperature-dependent fecundity were constructed, using the temperature-dependent adult traits. Temperature-dependent development models and adult oviposition models will be useful components to understand the population dynamics of O. scapulalis and will be expected using a basic data for establishing the strategy of integrated pest management in leguminous crops.

• Korean journal of applied entomology
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• v.48 no.3
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• pp.335-345
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• 2009

Effect of four nematicidal herbal extracts (Daphne genkwa, Eugenia caryophyllata, Quisqualis indica and Zingiber officinale) and 3 acricidal herbal extracts (Pharbitis nil, Xanthium strumarium, and Desmodium caudatum) on entomopathobenic nematodes [Steinernema carpocapsae Pocheon strain (ScP) and Heterorhabditis sp. Gyeongsan strain (HG)], silkworm (Bombyx mori), and ground beetles (Synuchus sp.) were investigated in the laboratory and field. D. genkwa was highly toxic to SCP and HG (100% mortality) at the concentration of 5,000 ppm in X-plate. All the infective juveniles of HG were dead after 3 days by E. caryophyllata and Q. indica. The mortality of ScP and HG was below 10% by D. genkwa, D. caudatum, E. caryophyllata, Q. indica and Z. officinale at the concentration of 1,000 ppm two days after treatment while mortality of HG was 62.8% by D. genkwa at the concentration of 1,000 ppm in X-plate. However, 1,000 ppm had not effect on nematode survival and pathogenicity of ScP in sand column. On the contrary, E. caryophyllata had effect on pathogenicity of HG. Mean number of dead Galleria mellonella larva of HG was 0.5 in E. caryophyllata treatment. Q. indica did not effect silkworm reared on mulberry leaves at the treatment of 1,000 ppm in 10 days after treatment. However, there were 20.0 and 100% mortalities in the treatment of D. genkwa 3 and 10 days after treatment, respectively. The weight of silkworm was low in D. genkwa and did not pupate. The weight of pupa and cocoon were not different in E. caryophyllata, P. nil, Q. indica, X. strumarium and Z. officinale. D. genkwa, E. caryophyllata, P. nil, Q. indica and Z. officinale had no effect on ground beetles, Synuchus sp. in forest soil.

• Korean journal of applied entomology
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• v.42 no.3
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• pp.217-223
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• 2003

Effects of temperature on the development and reproduction of the Luciola lateralis were investigated at various temperatures. The development time of eggs, larvae, and pupae were shorter at higher temperatures than at lower ones. The insect did not develop at 10$^{\circ}C$ and 35$^{\circ}C$. The hatchability was 61.5% at 15$^{\circ}C$, 73.9% at 20$^{\circ}C$, 93.3% at 23$^{\circ}C$, 91.8% at 25$^{\circ}C$, 74.0% at 27$^{\circ}C$, and 46.0% at 30$^{\circ}C$, indicating the best hatchability rate at the temperature condition of 23 DC. Larval periods were 341.5:t 23.2 days at 15$^{\circ}C$, 265.5${\pm}$17.5 days at 20$^{\circ}C$, and 250.9${\pm}$11.7 days at 25$^{\circ}C$. Pupal periods were 94.7${\pm}$11.5 days at 15$^{\circ}C$, 41.7${\pm}$9.1 days at 20$^{\circ}C$, and 18.5${\pm}$7A days at 25$^{\circ}C$. Emergence rate was 23.3, 89.3 and 80.7%, respectively at the above temperatures. Adult longevity of female was 18.0 days at 15$^{\circ}C$, 2004 days at 20$^{\circ}C$, 10.7 days at 25$^{\circ}C$, and 5.8 days at 30$^{\circ}C$. Mean fecundity per female was higher at 20$^{\circ}C$ compared with at other temperatures. The developmental zero point temperatures (1) and the total effect temperatures (I<) of egg, larva, pupa, and complete development were 10.6, 14.0, and l3.1$^{\circ}C$ and 214.8, 1,564.8, and 229.2 degree-days, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (Ra) was the lowest at the highest temperature as well as at the lowest, and it was 177.19 which was the highest at 23$^{\circ}C$. The intrinsic rate of natural increase (r$\sub$m/) was highest at 27$^{\circ}C$ as 0.019. As a result, optimum range of temperature for L. lateralis growth was between 20-25$^{\circ}C$.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.797-808
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• 2016

The experiment of grape leafroller (Herpetogramma luctuosalis) was carried out at Okcheon area from 2007 to 2009 and 2015. The grape leafroller had been occurred at a campbell early' organic vineyard in Okcheon. It's larva was rolling the leaf of grape and ate the leaf. So the leaf of grape decreased. In organic vineyards, adult grape leafroller' generation rate per year showed the first peak in mid-June, the second peak in early -August and the third in mid-September. The larva showed the first peak in early July and the second peak in late August-early September. The grape leaf roller had three generations per year. And it took $60.9{\pm}1.09days$ from egg to adult in growth chamber (VS-91G09M-1300) which the relative humidity conditions was $60{\pm}10%$, temperature $25{\pm}2^{\circ}C$ and photoperiod 16L:8D (The egg : $12{\pm}0days$, larvae : $22.2{\pm}0.22days$, pupa : $10.6{\pm}0.75days$ and adult : $16.1{\pm}0.45days$). It was conducted to find out the effect of microbial pesticide treatments to control H. luctuosalis. The 4 microbial pesticides (Bacillus thurigiensis) were treated twice on the grape leaves in June 11 and 21 at an organic vineyard in Okcheon. On 10 days after last treatment, the control value of all microbial pesticides were more than 95%. When the dates of spraying to the grape leaves were on May 22, June 12 and July 2 each, the effects of microbial pesticide were 73.9%, 93.5% and 43.6% respectively. As a result, it was effective that Bt was sprayed to grape leaves on mid and late June for controling the H. luctuosalis in organic vineyard. And microbial pesticide Bt was thought to be useful to control the grape leafroller in organic vineyard.