• 제목/요약/키워드: pulsed electric field

검색결과 105건 처리시간 0.027초

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

  • Liu, Di;Zhang, Ting;Chen, Zhifei;Wang, Ying;Ma, Shuang;Liu, Jiyun;Liu, Jingbo
    • Journal of Ginseng Research
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    • 제41권2호
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    • pp.169-179
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    • 2017
  • Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

Production of Ethanol Directly from Potato Starch by Mixed Culture of Saccharomyces cerevisiae and Aspergillus niger Using Electrochemical Bioreactor

  • Jeon, Bo-Young;Kim, Dae-Hee;Na, Byung-Kwan;Ahn, Dae-Hee;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제18권3호
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    • pp.545-551
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    • 2008
  • When cultivated aerobically, Aspergillus niger hyphae produced extracellular glucoamylase, which catalyzes the saccharification of unliquified potato starch into glucose, but not when grown under anaerobic conditions. The $K_m\;and\;V_{max}$ of the extracellular glucoamylase were 652.3 mg/l of starch and 253.3 mg/l/min of glucose, respectively. In mixed culture of A. niger and Saccharomyces cerevisiae, oxygen had a negative influence on the alcohol fermentation of yeast, but activated fungal growth. Therefore, oxygen is a critical factor for ethanol production in the mixed culture, and its generation through electrolysis of water in an electrochemical bioreactor needs to be optimized for ethanol production from starch by coculture of fungal hyphae and yeast cells. By applying pulsed electric fields (PEF) into the electrochemical bioreactor, ethanol production from starch improved significantly: Ethanol produced from 50 g/l potato starch by a mixed culture of A. niger and S. cerevisiae was about 5 g/l in a conventional bioreactor, but was 9 g/l in 5 volts of PEF and about 19 g/l in 4 volts of PEF for 5 days.

(Bi,Nd)(Fe,Ti)$O_3$ 다강체 세라믹 및 박막의 상변화 거동 (Phase Evolution Behavior of Multiferroic (Bi,Nd)(Fe,Ti)$O_3$ Ceramics and Thin Films)

  • 김경만;양판;이재열;이희영
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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    • pp.231-232
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    • 2008
  • The coupling between electric, magnetic, and structural order parameters results in the so-called multiferroics, which possess ferroelectricity, ferromagnetism, and/or ferroelasticity. The simultaneous ferroelectricity and ferromagnetism (magnetoelectricity) allow potential applications in information storage, spintronics, and in magnetic or electric field sensors. Perovskite compound $BiFeO_3$ (BFO) is antiferromagnetic below Neel temperature of 647K and ferroelectric with a high Curie temperature of 1043K. It exhibits weak magnetism at room temperature(RT) due to the residual moment from a canted spin structure. It is likely that non-stoichiometry and second-phase formation are the factors which cause leakage in BFO. It has been suggested that oxygen non-stoichiometry leads to valence fluctuations of Fe ions in BFO, resulting in high conductivity. To reduce the large leakage current of BFO, one attempt is fabricating donor doped BFO compounds and thin films. We report here the successful fabrication of the Nd, Ti co-doped $BiFeO_3$ ceramics and thin films by pulsed laser deposition technique.

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양방향 펄스전원을 이용한 NO 가스의 방전처리 (Discharge Processes of NO Gas Using Bidirectional Pulsed Voltage)

  • 주홍진;박정호;심재학;고광철;강형부
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2001년도 하계학술대회 논문집 C
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    • pp.1682-1684
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    • 2001
  • In removing the flue-gas using electrical discharge method, it is important to dissociate or ionize the atoms and molecules by the collisions with energetic electrons and it produces the radicals that are used to decompose the pollutants. For that purpose, a bidirectional pulsed voltage is used to produce lots of energetic electrons efficiently and increase the power efficiency. The simulation is performed with changing the pulsewidth under the fixed applied voltage. The particle-mesh model coupling the NGP(nearest-grid-point) to FEM(finite element method) is used to simulate the behavior of electrons and the spatio-temporal variation of the electric field for the streamer in discharge tube.

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Efficient Second Harmonic Generation of Pulsed Nd-YAG Laser Radiation with Noncritically Phase-Matchable $LiNbO_3$ in Room-Temperature

  • Jong-Soo Lee;Bong-Hoon Kang;Bum Ku Rhee;Chong-Don Kim;Gi-Tae Joo
    • The Korean Journal of Ceramics
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    • 제6권3호
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    • pp.206-208
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    • 2000
  • 0.65 mol% MgO-doped LiNbO$_3$single crystals were grown by CZ method. The obtained single crystals were colorless and transparent. Noncritically phase-matched second harmonic generation (SHG) of 532-nm radiation from 1064-nm in MgO-doped LiNbO$_3$has been investigated by using pulsed Nd:YAG laser. The phase-matching temperature was room temperature. SHG conversion efficiencies were typically achieved higher than 50% at the phase-matching temperature with no photorefractive damage in the region of fundamental power density which was used in this experiment. The thermo-birefringence coefficient and the electro-birefringence coefficient of SHG were calculated from the temperature phase-matching profile with the electric field.

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Electromigration Characteristics in PSG/SiO$_2$ Passivated Al-l%Si Thin Film Interconnections

  • Kim, Jin-Young
    • Journal of Korean Vacuum Science & Technology
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    • 제7권2호
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    • pp.39-44
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    • 2003
  • Recent ULSI and multilevel structure trends in microelectronic devices minimize the line width down to a quarter micron and below, which results in the high current densities in thin film interconnections. Under high current densities, an EM(electromigration) induced failure becomes one of the critical problems in a microelectronic device. This study is to improve thin film interconnection materials by investigating the EM characteristics in PSG(phosphosilicate glass)/SiO$_2$ passivated Al-l%Si thin film interconnections. Straight line patterns, wide and narrow link type patterns, and meander type patterns, etc. were fabricated by a standard photholithography process. The main results are as follows. The current crowding effects result in the decrease of the lifetime in thin film interconnections. The electric field effects accelerate the decrease of lifetime in the double-layered thin film interconnections. The lifetime of interconnections also depends upon the current conditions of P.D.C.(pulsed direct current) frequencies applied at the same duty factor.

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유전체를 활용한 초고속 에너지 충/방전 소자 기술 (Recent Progress in Dielectric-Based Ultrafast Charging/Discharging Devices)

  • 최현수;류정호;윤운하;황건태
    • 한국전기전자재료학회논문지
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    • 제35권4호
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    • pp.322-332
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    • 2022
  • Energy storage capacitors based on dielectric ceramics with superior polarization properties and dielectric constant can provide much higher output power density due to their very fast energy charging/discharging rates, which are particularly suitable for operating pulsed-power devices. For an outstanding energy storage performance of dielectric capacitor, a large recoverable energy density could be derived by introducing a slim polarization-electric field hysteresis loop into dielectric materials by various technical approaches. Many research teams have explored various dielectric capacitor technologies to demonstrate high output power density and ultrafast charging/discharging behavior. This article reviews the recent research progress in high-performance dielectric capacitors for pulsed-power electronic applications.

알루미늄과 구리 간 통전고상접합 연구 (A Study on Electrically Assisted Solid State Joining of Aluminum and Copper)

  • 박주원;최호욱;이시환;정혜진;홍성태;한흥남
    • 소성∙가공
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    • 제29권1호
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    • pp.49-54
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    • 2020
  • The influence of electric current on the joining properties of aluminum and copper was investigated. Various pulsed electric current conditions were set to the joining specimens followed by pressure. The shear strength of the joint area between aluminum and copper was measured by the lab shear test. In addition, the microstructures of the joint area were observed through a field emission scanning electron microscope, energy dispersive X-ray, and electron backscatter diffraction. The mechanical properties of each phase in the joint region were measured by nano-indentation. As a result, it was confirmed that electrically assisted solid state joining of copper and aluminum could be applied in various industrial fields.

Exciting High Frequency Oscillations in a Coaxial Transmission Line with a Magnetized Ferrite

  • Ahn, J.-W.;Karelin, S.Y.;Kwon, H.-O.;Magda, I.I.;Sinitsin, V.G.
    • Journal of Magnetics
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    • 제20권4호
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    • pp.460-465
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    • 2015
  • The paper presents methodologies and results concerning one- and two-dimensional numerical modeling of radio frequency oscillations in a coaxial transmission line fed with a short pulse of electric current. The line is partially filled with a ferrite material, magnetized longitudinally close to saturation. The 2D model has permitted analyzing, for the first time in the art, the spatial structure and dynamics of the wave field within the radially non-uniform cross-section planes of the non-linear and dispersive guiding structure. This opens ways for optimizing size parameters of the line and the extent to which it is filled with the ferromagnetic material, thus increasing the line's electric strength and intensity of the r.f. oscillations.