• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.127-135
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• 2020

Small RNAs (sRNAs) are widespread and play major roles in regulation circuits in bacteria. Previously, we have demonstrated that transcription of esrE is under the control of its own promoter. However, the regulatory elements involved in EsrE sRNA expression are still unknown. In this study, we found that different cis-regulatory elements exist in the promoter region of esrE. We then screened and analyzed seven potential corresponding trans-regulatory elements by using pull-down assays based on DNA affinity chromatography. Among these candidate regulators, we investigated the relationship between the ferric uptake regulator (Fur) and the EsrE sRNA. Electrophoresis mobility shift assays (EMSAs) and β-galactosidase activity assays demonstrated that Fur can bind to the promoter region of esrE, and positively regulate EsrE sRNA expression in the presence of Fe²⁺.

• Korean Journal of Human Ecology
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• v.16 no.6
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• pp.1243-1250
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• 2007

The purpose of this study was to compare government guidelines and foreign guidelines related to furniture design for elderly. Future elderly will increasingly accustomed to the provisions of improved housing, education and welfare in comparison with our forebears. Also, there will be upgrading and different need for elderly furniture in our market. The results were as follows. The height of bed was based on the height of wheelchair seat, commonly. A adjustable bed was recommended for abled and disabled elderly. A pull-down shelf, drawer, empty space under chest, folding or sliding door in wardrobe and so on were recommended as a elderly furniture. It were good adjustable sink on human scale. There were need the empty space under sink for wheelchair users, one-touch or lever faucet, etc. There have given a little furniture design guidelines for elderly in Korea until now. But those guidelines haven't been considered enough human scale of elderly including wheelchair user. Because the close investigations have not made about abled and disabled elderly for their furniture design. So first of all, the government should be examined elderly physical size more accurately for elderly furniture design. The guideline for elderly furniture design should be for abled elderly and the disabled, too.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.4
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• pp.199-204
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• 2008

KIF1B${\beta}$ is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors that are involved in various intracellular organellar transport processes. KIF1B${\beta}$ is not restricted to neuronal systems, however, is widely expressed in other tissues, even though the function of KIF1B${\beta}$ is still unclear. To elucidate the KIF1B${\beta}$-binding proteins in non-neuronal cells, we used the yeast two-hybrid system, and found a specific interaction of KIF1B${\beta}$ and the sorting nexin (SNX) 17. The C-terminal region of SNX17 is required for the binding with KIF1B${\beta}$. SNX17 protein bound to the specific region of KIF1Bf3 (813-916. aa), but not to other kinesin family members. In addition, this specific interaction was also observed in the Glutathione S-transferase pull-down assay. An antibody to SNX17 specifically co-immunoprecipitated KIF1B${\beta}$ associated with SNX17 from mouse brain extracts. These results suggest that SNX17 might be involved in the KIF1B${\beta}$-mediated transport as a KIF1B${\beta}$ adaptor protein.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.13 no.3
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• pp.239-254
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• 1988

In this paper an exciter which performs modulation and amplification is composed of high power(30dBm) VCO(Voltage Controlled Oscillator) using push-pull circuit. Modulation is FSK using PLL(Phase Locked Loop). A single loop PLL synthesizer having sequency range of 42.5-100.5MHz, 25KHz channel spacing and switching time of 1msec converts down the exciter VCO frequency to 1.25MHz. This signal mixed with the FSK modulated signal coming in the phase detector of exciter. The acquisition time of exciter for frequency hoppng is less than 200usec, so the total acquisition time for transmission is less that 1.5msec. There is no need of additional power amplification because power amlifiction by high power VCO is high enough to communicate within near distance. The proposed frequency synthesizer is not complex so it is suitable for low cost slow frequency hopping spread spectrum communication.

• Parasites, Hosts and Diseases
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• v.45 no.3
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• pp.165-174
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• 2007

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar component of the nucleolus morphologically by the overlap of GFP-GRA10 transfection image with IFA images by monoclonal antibodies against GRA10 (Tg378), B23 (nucleophosmin) and C23 (nucleolin). The nucleolar translocalization of GRA10 was caused by a putative nucleolar localizing sequence (NoLS) of GRA10. Interaction of GRA10 with TATA-binding protein associated factor 1B (TAF1B) in the yeast two-hybrid technique was confirmed by GST pull-down assay and immunoprecipitation assay. GRA10 and TAF1B were also co-localized in the nucleolus after co-transfection. The nucleolar condensation of GRA10 was affected by actinomycin D. Expressed GFP-GRA10 was evenly distributed over the nucleoplasm and the nucleolar locations remained as hollows in the nucleoplasm under a low dose of actinomycin D. Nucleolar localizing and interacting of GRA10 with TAF1B suggested the participation of GRA10 in rRNA synthesis of host cells to favor the parasitism of T. gondii.

• Computational Structural Engineering
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• v.6 no.1
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• pp.107-115
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• 1993

This study is directed for the development of a computer aided safety assessment system for agricultural irrigation structures. The developed system is composed of four subsystems that incorporate database, structural analysis, safety assessment, and postprocess, which are made to be interfaced systematically. It is developed in the user-friendly menu driving form with pull-down type interaction on a personal computer. The main algorithm for safety assessment of deteriorated structures utilizes a rational rating system based on the reliability method. From this study, it may be stated that the proposed rating and safety assessment system provide an effective tool and thus it can be widely used in practice for the assessment of safety and load carrying capacity of existing deteriorated or damaged agricultural irrigation structures.

• Journal of the Korean Institute of Rural Architecture
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• v.16 no.1
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• pp.111-117
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• 2014

The aim of this study is to find out the pattern of Ger form, inner spatial composition of Mongolian Ger house, and to take a dig at the structural or symbolic elements of nomadic architecture of Ger. To the point of view of corresponding to living and space of housing, remarkable characteristics of Ger Architecture is able to pull down and recombine the structures of nomadic house. Even though urbanization of Mongolia has spreading rapidly in a whole nation, most of people preserves traditional housing pattern within Ger. The ways of survey are to study of traditional home of Mongolia, and then field work at residence or mountain area in Ulan Bator and Gorkhi Terelj National Park area. This survey contains the form, size, structure, spatial composition of living space, structure, and materials. There are three results as follows: First, the form of Ger house is like a pyramidal or crown roof style to approximate to the round shape of it. Usually they had lived in nomadic way of life, so the Ger had a movable and flexible structure. Second, the Ger is easy to build up and deconstruct to move or find a new pasture. Third, the Mongolian Ger structure is composed by mainly five elements that are Khana, Khaalga, Toono, Bagana, and Uni. It has a hierarchy of internal spaces which are classified to gender, orientation, and property.

• Plant Biotechnology Reports
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• v.3 no.2
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• pp.127-134
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• 2009

Members of the NAM-ATAF-CUC (NAC) protein family are plant-specific transcription factors that contain a highly conserved N-terminal NAC-domain and diverse C-terminal regions. They have been implicated in plant development and abiotic stress responses. To identify interacters of rice NAC-domain proteins (OsNACs), we performed yeast two-hybrid screening of rice cDNA library using OsNAC5 as a bait, and the results showed that OsNAC5 interacts with other OsNACs including itself. To delineate an interacting domain, a series of deletion constructs of four OsNACs were made and transformed into yeast in various combinations. The results revealed that the conserved NAC domain of OsNACs plays a primary role in homodimer and heterodimer formation, and a part of C-terminal sequence is also necessary for the interaction. In vitro pull-down assays using recombinant OsNAC proteins verified the dimer formations, together suggesting that OsNACs may act by forming homodimers and/or heterodimers in plants.

• Proceedings of the KSR Conference
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• 2008.06a
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• pp.2139-2144
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• 2008

There are eight kinds of railroad vehicles such as KTX, PP(Push-Pull), NEL(New Electric Locomotive), EL(Electric Locomotive), DL(Diesel Locomotive), CDC(Commuter's Diesel Car), VVVF(Variable Voltage Variable Frequency) and Resistance Control Car that Korail corporation presently runs, and a variety of vehicles just like EMU(Electric Multiple Unit) and DMU(Diesel Multiple Unit) currently developed and accepted are running in the near future. However, There is still no design standard of the control stand of cockpit and the same compatibility of forms and control unit arrangements for locomotive engineers because no one has tried to approach in an ergonomic way. It can cause Locomotive engineers to make errors using the machinery. when the new vehicles are adopted, The efficiency of operation will quite fall down due to the separate training of the engineers. Therefore, We'd like to improve the accuracy of manipulating the machinery used by the engineers at all times according to the design standard of ergonomic technology and safety engineering and increase the operational efficiency and the safety of railroad vehicles in order to handle the problems as quickly as we can in an emergent situation.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3241-3246
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• 2010

NHERF ($Na^+/H^+$ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent $K_D$ of 7 nM. Fluorescence emission spectra of the NHERF-E3KARP complex suggested that the tight interaction between these proteins was accompanied by significant conformational changes in one or both. The CD spectra of NHERF and E3KARP show that the conformational changes of these proteins were dependent on pH and temperature. These results implicate that the NHERF-E3KARP complex allows intracellular signaling complexes to form through PDZ-PDZ interactions.