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A Blockade of the Central MAPK Pathway Attenuates Referred Pain in Rats with Complete Freund's Adjuvant -Induced Inflammation of the Temporomandibular Joint

  • Won, Kyoung-A.;Lim, Nak-H.;Lee, Min-K.;Park, Min-K.;Yang, Gwi-Y.;Park, Yoon-Yub;Ahn, Dong-K.;Bae, Yong-C.
    • International Journal of Oral Biology
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    • v.35 no.3
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    • pp.83-89
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    • 2010
  • We investigated the role of the central MAPK pathways in extra-territorial (referred) pain resulting from inflammation of the temporomandibular joint (TMJ). Experiments were carried out on male Sprague-Dawley rats weighing 220-280 g. Under anesthesia, these animals were injected with $50\;{\mu}L$ of complete Freund's adjuvant (CFA) into the TMJ using a Hamilton syringe. In the control group, saline was injected into the TMJ. To identify the extent of inflammation of the TMJ, Evans blue dye (0.1%, 5 mg/kg) was injected intravenously at 1, 3, 6, 9, 12 and 15 days after CFA injection. The concentration of Evans blue dye in the extracted TMJ tissue was found to be significantly higher in the CFA-treated animals than in the saline-treated group. Air-puff thresholds in the vibrissa pad area were evaluated 3 days before and at 3, 6, 9, 12, 15 and 18 days after CFA injection into the TMJ. Referred mechanical allodynia was established at 3 days, remained until 12 days, and recovered to preoperative levels at 18 days after CFA injection. This referred mechanical allodynia was observed in contralateral side area. To investigate the role of central MAPK pathways, MAPK inhibitors ($10\;{\mu}g$) were administrated intracisternally 9 days after CFA injection. SB203580, a p38 MAPK inhibitor, significantly attenuated referred mechanical allodynia, as compared with the vehicle group. PD98059, a MEK inhibitor, also reduced CFA-induced referred mechanical allodynia. These results suggest that TMJ inflammation produces extra-territorial mechanical allodynia, and that this is mediated by central MAPK pathways.

Ash Drop Measuring on Tobacco Leaf Grades and Brand Cigarettes (잎담배 및 제품담배의 재떨어짐에 관한 연구)

  • Yang Burm-Ho;Ahn Dae-Jin;Jo Si-Hyung;Jeh Byong-Kwon;Kim Si-Mong;Kim Byeoung-Ku
    • Journal of the Korean Society of Tobacco Science
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    • v.26 no.2 s.52
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    • pp.117-125
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    • 2004
  • In this study, we have investigated the natural and puffing combustibility of cigarette brands and the compulsory ash-drop, and analysed factors related to the materials. Cigarette combustibility was greatly affected by the tobacco weight in cigarettes rod. Combustion rate of 4AOR cigarette made by flue-cured tobacco was 5.02 $^{mm}/_{min}\;and\;10\%$ faster than 1BO cigarette. Combustion pattern has interrelation with sugars, chlorine and ash contents among tobacco components. Combustion rate of 4DL, flue-cured tobacco was 55 seconds faster than 1CL tobacco extremely much in sugars contents, and ash drop was maintained at $21\%$ longer. Combustion time and burning rate were fast in cigarette made by expanded tobacco slice and tobacco stem and the formation of ash was poor. The length of puffed ash drop of expanded tobacco slice and tobacco stem were 18.6 mm and 25.2 mm and these forced ash drop length were 3.8 mm and 4.9 mm respectively. Puffed burning rate of general cigarette was $14.3\%$ faster than static burning rate and super slim type cigarettes was $21.3\%$ faster. Combustion rate and ash drop of domestic and foreign cigarette brands were quite different depending on tar level and cigarette types. The lower tar level, the faster combustion rate and the shorter ash drop.

A Study on Wedding Dress Design of popular stars in Korea (한국 대중스타의 시대별 웨딩드레스 디자인 분석)

  • Lee, Eun-Jung;Chang, Geung-Hae
    • Journal of the Korea Fashion and Costume Design Association
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    • v.8 no.2
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    • pp.85-94
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    • 2006
  • In Korea, popular stars were the leaders of the latest fashion, who had the strong power in apparel market. Costume, especially wedding dress they worm became the focus of interest to single women of marriageable age and wedding dress co. This study was performed to research wedding dress design (silhouette, veil, sleeves, materials, and details) from 1960's to June. 2004. Resources were extracted from the weddings photos, 'My Wedding' magazine, 'Vogue Korea' magazine and web sites. The materials, which could not be judged on the photos, were researched from text articles. The popular stars were TV stars, musicians, and actresses. The results of this study were as follows: The wedding dress in Korea from 1960's to June 2004 had different distinguishing characteristics. 1. In 1960's, wedding dress was characterized with A silhouette, long veil, set in sleeves, and hair decoration. 2. In 1970's, H silhouette decorating by belt, long veil, puff sleeves, and ruffles hemline were in vogue. 3. In 1980's, Victorian style designed by long and wide width, the leg of mutton sleeves or tightly fitted sleeves, short veil and pearl coronet, flower decoration became popular. 4. In 1990's, to reach the elegance or romantic image, it was used race, ribbon, frill, etc., on the base of 1980's wedding dress design. Generally the girlish styles which expressed innocent and pure and feminine were in fashion. 5. In 2000's, the keyword of both luxury and simple were intended. Silhouette was designed variously, but it became slimmer and simpler. The decoration was splendorous with race, embroidery, and beads.

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A Study on Draping and Making up of Spencer Jacket in the Empire Style(1789-1820) (엠파이어 스타일 시대[1789-1820] 스펜서 재킷 제작에 관한 연구)

  • Choi Mi-Kyung;Jo Jin-Sook;Choi Jin-Hee
    • Journal of the Korean Society of Costume
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    • v.56 no.4 s.103
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    • pp.48-64
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    • 2006
  • The purpose of the study was to demonstrate how to recreate spencer jacket in the empire style in an attempt to apply this historic style to theatrical costumes and other high fashion items in modern times. The study was carried out through 2 steps. (1)Block patterns of spencer jacket of its basic design were developed through draping based on the design characteristics mentioned above. (2)Spencer jackets of three designs, which were the typical designs of each category have been made of velvet to find out more specific structural characteristics. The structural characteristics of Spencer jacket in draping were as follows: (1)Jacket length position was suitable 9cm below body's bust line and neckline should be enlarged front & side neck point 2cm, back neck point 1.5cm. (2)Cut out the front bodice after CF line moved 1cm outwards and bust dart amount should not exceed 2-3cm. Shoulder point moved inwards. (3)Side back grain line was parallel to princess line of the back bodice. Add 0.5cm ease to the under arm of the front and side bark bodice, it was good for the activity of arm. (4)Armhole line was trued as maintaining to across back width 14-15cm at least. Shoulder line was to connect from the point 2cm depart shoulder point along armhole line to point 2cm side neck point along neck line. (5)Sleeve length was suitable 70cm, cut out the bias direction to move forwards the grain line from shoulder point. Puff position was good from the 2cm depart shoulder point to shoulder line.

A Study on Hair and Make-up Design -Focus on the Hair and Make-up Trend Since the 2003s- (헤어·메이크업 디자인 연구 -2003년 이후에 나타난 헤어·메이크업 경향을 중심으로-)

  • Shon, Young-Mi;Lee, Hae-Jung
    • Fashion & Textile Research Journal
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    • v.7 no.6
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    • pp.657-664
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    • 2005
  • Man's search for beauty has been going on from prehistoric time until today, and the pursuit of beauty has been progressing, regardless of the times, as a human psychological desire. In the search for beauty, hair and make-up have been subjects of interest for a long time, but the development of designs for these two aspects are still behind compared to other areas. Therefore, this study aims to propose various new and creative hair and make-up designs and to suggest their possible development. Using existing literature, this paper studied the skin color and hair color of Koreans that had been studied in earlier papers. It also aimed to understand the hair color and hair color images that are suitable for each skin color, classify the modern hair and make-up trends into different images - natural, surrealistic, minimalist, and cyber look, and also analyze them. Actual hair and make-up were also designed based on these images. To depict the natural image, the make-up was made intentionally dark as the skin color; natural materials were used for hair decoration; brown and yellow were used for the eye shadow to express the natural and romantic image of autumn, in general. Second, in the work expressing the surrealistic image, the hair was creatively decorated with a collage of various objects; yellow, green, and red eye shadows and lip expression were used to visually achieve the surrealistic fantasy look. Thirdly, in the work expressing the minimalist image, hair style with bangs without any hair decoration was used; make-up colors were minimized as well, creating a generally simple image. Finally, for the work expressing the cyber image, metallic material decoration was used on a puff-up hair style; the cyber image was emphasized through the use of graphical elements in the make-up.

Effect of Aging Period on Chromatic, Chemical and Organoleptic Properties of Burley Leaf Tobacco (버어리종 잎담배의 숙성기간이 색상, 화학성분 및 끽미에 미치는 영향)

  • 안대진;정기택;이종률;제병권;조수헌
    • Journal of the Korean Society of Tobacco Science
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    • v.26 no.1
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    • pp.35-42
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    • 2004
  • This study was carried out to examinate the effect of aging period on chromatic, chemical and organoleptic characteristics, and to evaluate of optimum aging period for each grade in burley leaf tobacco. The leaf tobaccos were produced in 2000, and threshed, redried and packed in carton box under the current methods. Four grades of processed leaf(A3T, B1T, C1W and D3W) were stored during 24 months (May 10, 2001 to April 31, 2003) in warehouse of Chungju Leaf Tobacco Processing Factory. The leaf tobaccos were sampled at three month intervals for analysis of chromatic, chemical and organoleptic properties. pH values of four grades, and Yellow(b) of A3T and BIT were also significantly decreased during the aging. Filling values, tar, nicotine and CO contents of tobacco smoke, and puff number of cigarettes were not significantly changed during the aging. Positive correlation coefficients were significantly observed between irritation of the calculated attributes from contents of volatile oil components in leaf tobacco and that of the panel sensory attributes. The ratio of maximum change in irritation attribute was larger than that in taste attribute during aging. The calculated irritations of 18~24 months aging for A3T and B1T were significantly lower than zero-aging, while those of C1W and D3W were not significant among aging periods. The panel irritations of 12~15 months aging for four grades were low tendency. The results suggest that decrease of aging period for thin leaf from 21~24 to 12~15 months may be beneficial to save storage cost in burley tobacco.

Effect of Aging Period on Chromatic, Chemical and Organoleptic Properties of Flue-cured Leaf Tobacco (황색종 잎담배의 숙성기간이 색상, 화학성분 및 끽미에 미치는 영향)

  • 정기택;안대진;이종률;제병권
    • Journal of the Korean Society of Tobacco Science
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    • v.26 no.1
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    • pp.27-34
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    • 2004
  • This study was carried out to examinate the effect of aging period on chromatic, chemical and organoleptic characteristics, and to evaluate of optimum aging period for each grade in flue-cured leaf tobacco. The leaf tobaccos were produced in 2000, and threshed, redried and packed in carton box under the current methods. Four grades of processed leaf(A3O, B1O, C1L and D3L) were stored during 24 months(May 10, 2001 to April 31, 2003) in warehouse of Chungju Leaf Tobacco Processing Factory. The leaf tobaccos were sampled at three month intervals for analysis of chromatic, chemical and organoleptic properties. Yellow(b), pH values and total sugar contents of four grades were significantly decreased during the aging. Filling values, tar, nicotine and CO contents of tobacco smoke, and puff number of cigarettes were not significantly changed during the aging. Positive correlation coefficients were significantly observed between taste and irritation of the calculated attributes from contents of volatile oil components in leaf tobacco and those of the panel sensory attributes. The ratio of maximum change in taste attribute was larger than that in irritation attribute during aging. The optimum aging periods estimated by taste for A3O, B1O, C1L and D3L were 17.8, 14.9, 10.8, and 9.8 months, respectively. The thin leaf(Primings and Cutters) undergo satisfactory aging earlier as compared to bodied leaf(Leaf and Tips). The results suggest that decrease of aging period for thin leaf from 18~21 to 9~12 months may be beneficial to save storage cost in flue-cured tobacco.

Statistical approach for development of objective evaluation method on tobacco smoke

  • Hwang, Keon-Joong;Rhee, Moon-Soo;Ra, Do-Young
    • Journal of the Korean Society of Tobacco Science
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    • v.22 no.2
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    • pp.184-189
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    • 2000
  • This study was conducted to develop the objective evaluation method for tobacco smoke. The evaluation was carried out by using the data of cut or blended tobacco components, smoke components, electric nose system (ENS), and sensory test. By using the statistical methods, such as cluster analysis, discriminant analysis, factor analysis, correlation analysis, and multiple regression analysis, the relationship among the data of tobacco, smoke, ENS, and sensory evaluation was studied. By the results of cluster analysis, the data from smoke analysis by GC and ENS were able to select the difference of tobacco leaf characteristics. As the results of discriminant analysis, grouping by the components of tobacco leaves and smoke was possible and the results of GC analysis of smoke could be used for discrimination of tobacco leaves. In the results of factor analysis, nicotine, tar, CO, puff No and pH in the smoke were the factors effecting on the tobacco leaf characteristics. From the correlation analysis, aroma, taste, irritation, and smoke volume of sensory test had high relation to tar, p-cresol threonolatone, levoglucosane, and quinic acid- ${\gamma}$ -lactone of smoke. The ENS data showed high efficiency for discriminant analysis and cluster analysis, but it was not good for factor analysis, and correlation analysis. It was possible to estimate tobacco leaves and their blending characteristics by the analytical data of tobacco leaves, smoke, ENS, and sensory test results. By the multiple regression analysis, some correlation among selected chemical components and sensory evaluation were found. This study strongly indicated that the some chemical analysis data was available for the objective evaluation of tobacco sensory attributes.

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Analysis of Dress Style and Patterns for the Reproduction of Empire Style Dress (엠파이어 시대의 드레스 재현을 위한 형태분석 및 패턴연구)

  • Lee, Jeong-Ran;Jung, Jae-Min;Cho, Youn-Joo
    • Fashion & Textile Research Journal
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    • v.8 no.6
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    • pp.687-696
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    • 2006
  • This study aims at analyzing the silhouette, dress patterns and detail of Empire Era(1789-1825), and making use of this results for the modern stage costume construction. The dresses of Empire era were divided into X and H style. The results revealed that the bust girth of X style group was bigger than that of H style group. Such patterns as high waistline, puff sleeve, train, etc. which appeared during the peak time of Empire style period were more remarkable for H style group. This obviously indicated that more epical characteristics were observed for H style group than for X style group. Similarly to silhouette analysis, based on the results from cluster analysis conducted to put similar groups together, two groups were formed - a visually differentiated and complex group and a simple group in the absence of detail. Most dress details from Empire period had simple embellishments. Comparison of the average size of the patterns between two groups indicated that waist front length of H style was shorter than that of X style. Front interscye was even greater than back width for both groups. Such trend was quite different from today's pattern in which back width is larger than front width based on body measurements. Preference for big breast during the period was reflected on dress patterns and the side length of front side goes across the back much further for bodice patterns. Two representatives were selected and reproduced using materials similar to those of real dresses of Empire style, following two times fit test.

Trigeminal Neuralgia like Pain Behavior Following Compression of the Rat Trigeminal Ganglion

  • Yang, Gwi-Y.;Mun, Jun-H.;Park, Yoon-Y.;Ahn, Dong-K.
    • International Journal of Oral Biology
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    • v.34 no.3
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    • pp.157-164
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    • 2009
  • We recently described a novel animal model of trigeminal neuropathic pain following compression of the trigeminal ganglion (Ahn et al., 2009). In our present study, we adapted this model using male Sprague-Dawley rats weighing between 250-260 g and then analyzed the behavioral responses of these animals following modified chronic compression of the trigeminal ganglion. Under anesthesia, the rats were mounted onto a stereotaxic frame and a 4% agar solution ($10{\mu}L$) was injected in each case on the dorsal surface of the trigeminal ganglion to achieve compression without causing injury. In the control group, the rats received a sham operation without agar injection. Air-puff, acetone, and heat tests were performed at 3 days before and at 3, 7, 10, 14, 17, 21, 24, 30, 40, 55, and 70 days after surgery. Compression of the trigeminal ganglion produced nociceptive behavior in the trigeminal territory. Mechanical allodynia was established within 3 days and recovered to preoperative levels at approximately 60 days following compression. Mechanical hyperalgesia was also observed at 7 days after compression and persisted until the postoperative day 40. Cold hypersensitivity was established within 3 days after compression and lasted beyond postoperative day 55. In contrast, compression of the trigeminal ganglion did not produce any significant thermal hypersensitivity when compared with the sham operated group. These findings suggest that compression of the trigeminal ganglion without any injury produces prolonged nociceptive behavior and that our rat model is a useful system for further analysis of trigeminal neuralgia.