• Korean Journal of Pharmacognosy
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• v.14 no.4
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• pp.170-174
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• 1983

To explain the medical effect of 'Pyungwijiyu-Tang' which is mostly used in excremental blood caused by Eum-Gyul(陰結) in oriental medicine. The plasma prothrombin time on mouse dosed 'Pyungwijiyu-Tang', 'Gami-Pyungwjiyu-Tang and Schizonepetae Herba (parched) were revealed the shorten effect, but the plasma prothrombin time on mouse dosed Zingiberis Rhizoma (parched) was not revealed the shorten effect.

• Journal of Sasang Constitutional Medicine
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• v.2 no.1
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• pp.199-211
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• 1990

In order to investigate the hemostatic effect of Gamiikwiseungyangtang, prothrombin time was measured after administered with the extract of Ikwiseungyangtang added four several herb drugs and Ikwiseungyangtang extract added four several herb drugs extract to mice orally. The results were as follows: 1. Gamiikwiseungyangtang was recognized significance on the plasma prothrombin time in normal mice. 2. Gamiikwiseungyangtang was recognized significance on the plasma prothrombin time in hypoprothrombinemic mice. 3. Prothrombin time of Gamiikwiseungyangtang on normal and hypoprothrombinemic mice was not recognized effective difference according to two types. From above results, Gamiikwiseungyangtang indicates that it is effect on the hemostatic activity.

• Journal of Chest Surgery
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• v.2 no.1
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• pp.105-114
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• 1969

This experiment was carried out to study the responses of cellular component of blood and bone marrow to cold and also the changes of coagulation during cooling. Forty-two mongrel dogs were subjected to hypothermia by ice-water surface cooling technique. Lowest body temperature ranged from 21-23 degree. Dogs were divided into 3 groups,Group I, 12 dogs: pentothal anesthesia for 3 hours, Group II, 20 dogs;hypothermic group and Group III,10 dogs;postsplenectomy hypothermic group. Results were summarized as follows: 1. Hemoglobin, hematocrit and red blood cell count significantly increased when animals were cooled, and increase was noted in similar magnitude among the animals of Group I. 2. White blood cell count extremely decreased after cooling and effect of splenectomy on white blood cell count was not apparent. No significant changes were seen among Group I. 3. Differential count of white blood cell when cooled showed relative increase of polymorphonuclear neutrophil and decrease of lymphocyte. 4. There was marked decrease of platelets when body temperature reached to 21-23degree and essentially. no changes was noted in Group I. 5. Clotting time, bleeding time, plasma prothrombin time, recalcification time, and fibrinolysis showed no significant changes when dogs were cooled. Clot retration and prothrombin consumption during hypothermia appeared to be poor. In Group II, bleeding time decreased after splenctomy and when body temperature was lowered, plasma prothrombin time, clot retraction, and prothrombin consumption decreased. Decreased bleeding time and poor clot retraction were noted in Group I. 6. It was found that megacaryocyte count decreased even though platelet count of peripheral blood markedly diminsished when animals were cooled. There was some tendency of erythroid hyperplasia noted during hypothermia.

• Korean Journal of Pharmacognosy
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• v.19 no.1
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• pp.39-46
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• 1988

Experimental studies were conducted to investigate the hemostatic effect of the water extracts of Kwibitang. For this purpose, the effects of the extracts on the bleeding time in mouse tail and prothrombin time in vitro were estimated. Furthermore, its activity on the isolated uterine muscle in rats were investigated. The results obtained were as following; The bleeding time was not shortened, but the plasma prothrombin time in vitro test was significantly shortened. The uterotonic action produced by the extract was not inhibited by pretreatment of diltiazem at the doses of $10^{-5}g/ml$.

• Korean Journal of Pharmacognosy
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• v.19 no.1
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• pp.47-52
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• 1988

In an attempt to investigate the effect of Beekeumsan on the hemostatic action and isolated uterine muscle, Beekeumsan was administered orally and the bleeding time in mouse tail, prothrombin time in vitro were estimated. Its activity on the isolated uterine muscle in rats were investigated. The following results were obtained; The bleeding time was not shortened, but the plasma prothrombin time in vitro was significantly shortened. The uterotonic action produced by the Beekeumsan was not inhibited by pretreatment of atropine, but was slightly inhibited by cyproheptadine and completely inhibited by pretreatment of diltiazem.

• The Journal of Dong Guk Oriental Medicine
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• v.2 no.1
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• pp.155-166
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• 1993

Geukhachukeutang is used to cure blood stasis in Oriental Medicine. In order to investigate medical value on intravascula coagulation and contusion blood stasis, the effect of Platelet number, Fibrinogen quantity, Prothrombin time, FOP density, Paw edema increasing rate after prescribing Geukhachukeutang concentration liquid orally were observed. The obtained results are summerized as follows ; 1. Platelet number and Fibrinogen quantity are significantly increased, and Prothrombin time and FOP density are significantly decreased by Geukhachukeutang after inducing intravascula coagulation. 2. Platelet number and Fibrinogen quantity are not significantly increased, and Prothrombin time is not significantly decreased either, by Geukhachukeutang after inducing contusion blood stasis but Paw edema increasing rate is remarkablely decreased in three or four hours of the groups of prescribing. According to the above results, Geukhachukeutang will be used for contusion blood stasis and Intravascula coagulation effectively owing to being accepted as a favorable operation on Platelet number, Fibrinogen quantity, Prothrombin time, FDP density and Paw edema increasing rate.

• The Journal of Internal Korean Medicine
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• v.10 no.1
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• pp.137-155
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• 1989

In order investigate the effect of Boyanghwanotang (BT) on thrombosis, Sprague-Dawley rats injected endotoxin into the caudal vein were oral administrated solid extract of BT-182. 4mg/200g (Sample A) and 364.8mg/200g (Sample B), and then observed Platelet, fibrinogen, prothrombin time and FDP. Another group injected hydrocortisone acetate into the muscular rump were adopted to observe the effect of BT on elevated blood Viscosity, administrated Sample A and Sample B, thereafter measured body weight, body temperature, whole blood viscosity, plasma viscosity, hematocrit, RBC, WBC, hemoglobin, glucose, albumin, total protein, triglyceride, cholesterol and prothrombin time. 1. Platelet was increased significantly in Sample B compared with the control group. 2. Fibrinogen of the trial groups compared with the control group was increased, and revealed a significance in Sample B. 3. Prothrombin time was decreased significantly in the trial groups compared with the control group. 4. FDP was decreased in the trial groups compared with the control group. 5. Body weight was increased significantly in Sample B after 7 days, body temperature revealed a significance in the trial groups after 5 days. 6. Whole blood viscosity and plasma viscosity of lower shear rates, both were highly decreased in the trial groupn than the control group. 7. The increase of WBC and decrease of hematocrit, RBC and hemoglobin was showed in the trial groups, and Sample B revealed a significance on the increase of WBC and decrease of RBC. 8. The increase of prothrombin time and decrease of glucose, albumin, total protein, triglyceride and cholesterol was showed in the trial group, and Sample B revealed a Significance on the increase of prothrombin time and decrease of albumin, total protein and triglyceride. 9. As compare with Sample A and Sample B, generally Sample B was highly Significant. According to the above results, it was considered that Boyanghwanotang may be the curative effects for thrombosis and elevated blood viscosity.

• Journal of Society of Preventive Korean Medicine
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• v.2 no.1
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• pp.165-173
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• 1998

In order to investigate the effect of the Herba Segesbeckiae on the thrombosis induced by endotoxin in rats. The rats were treated with solid extract of Herba Siegesbeckiae 41.9mg /200g(Sample) which were administered orally. And the thrombosis was induced by injecting endotoxin in the caudal vein rats. And then the number of platelets, concentration of fibrinogen, prothrombin time and FDP(fibrinogen degradation product) were measured. The results were as follows; 1. Platelet was increased significantly in sample group compared with control group. 2. Prothrombin time was shortened significantly in sample group compared with control group. 3. Fibrinogen was increased significantly in sample group compared with control group. 4. FDP was decreased significantly in sample group compared with control group. According to the above results, Herba Siegesbeckiae had significant effects on number of Platelets, Prothrombin time, Fibrinogen and FDP. therefore, it is considered that Herba Siegesbeckiae can be applied to diseases related to thrombosis.

• The Journal of Korean Medicine
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• v.21 no.4
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• pp.47-54
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• 2000

Objectives: The purpose of this study was to investigate the antithrombotic effect of Carthamus tinctorius. Methods: SD rats were used to investigate the inhibitive effect of platelet aggregation (in vitro & in vivo), prothrombin time, platelet count. Mice were used to investigate the survival rate after injection of collagen & epinepbrine. Results: 1. The inhibitive effect of platelet aggregation (in vitro & in vivo) were increased significantly in all groups. 2. Prothrombin time was decreased in both groups. 3. Platelet count was decreased significantly in sample A. 4. Survival rate after injection of collagen & epinepbrine was increased in sample B. Conclusion: According to the above results, it is suggested that Carthamus tinctorius has antithrombotic effects.

• Korean Journal of Optics and Photonics
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• v.24 no.6
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• pp.338-341
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• 2013

Prothrombin Time (PT) is used as a measure of blood coagulation time. An optical analysis of the signal generating mechanism is described here for the reflection-type arrangement. Thromboplastin added into serum or whole blood initiates the coagulation process. We added a sufficient amount of magnetic particles into the sample before adding thromboplastin and subjected the mixture to a rotating magnetic field. The laser light gets reflected in a periodically modulated fashion with respect to the magnetic field rotation. Analysis of this decaying modulation could produce a reasonable coagulation time. We also introduce a simulation model to explain the signal generating mechanism using LightTools.$^{TM}$