• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.47-53
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• 1997

Appropriation of partial substitution of concentrate mixture by urea-molasses-mineral block (UMMB) lick supplements for 20 lactating crossbred cows in 2nd and 3nd lactation was studied. Animals fed on wheat straw ad lib. and Berseem (Trifolium alaxandrium) fodder @ 1.5 kg/d on dry matter basis. Animals of control group were given concentrate supplement, while in treatment groups 10% of the concentrate requirement was substituted with 3 different types of UMMB lick type A ($T_1$), type B ($T_2$) and type C ($T_3$). CP content of the ration was 15%. Total dry matter intake (DMI) was about 1.0 kg/kg of fat corrected milk (FCM) yield and was not significantly different between control and treatment groups. Digestibility of neither proximate principles nor cell wall constituents were deviated on UMMB licks partial supplementation. FCM yield was increased by 140, 410 and 460 g/d, in $T_1$, $T_2$ and $T_3$, respectively, in comparison to control group but differences were statistically invalid. Though fat per cent was reduced, fat yields were remain constant among treatments. Milk composition was unaltered except significant difference (p < 0.01) in non-protein nitrogen (NPN) content. Gross-N and digestible-N conversion was significantly higher (p < 0.01) with $T_1$, $T_2$ and $T_3$ than control group. Energy utilization efficiency for milk production was only 36%. Result demonstrated that UMMB licks could be partial supplemented up to 10% of the concentrate requirement of crossbred cows yielding on an average 14kg/d without any adverse effect on feed intake, nutrient utilization and mild production. Comparatively, UMMB lick type B and C was proved better than type A and also economically viable.

• Asian-Australasian Journal of Animal Sciences
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• 제9권2호
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• pp.223-229
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• 1996

This experiment was conducted to investigate the protein sparing effect of rumen protected lysine(RPLys) and methionine hydroxyl analogue(MHA) in sheep. The treatments were $T_1$ (CP 15% + RPLys 0%), $T_2$ (CP 12% + RPLys 0%), $T_3$ (CP 12% + RPLys 0.4%) and $T_4$ (CP 12% + RPLys 0.4% + MHA 0.3%). Ruminal characteristics, in situ and in vitro digestibility and nitrogen retention rate were measured in sheep receiving different combinations of dietary supplement. The results are summarized as follows: 1. Ruminal pH and VFA concentrations were not affected by the treatments. Ruminal ammonia-N concentration was high in sheep fed diets $T_2$, $T_3$ and $T_4$ with the highest value in the $T_4$ treatment(p<0.05). 2. The digestibilities of dry matter and organic matter were not affected by the treatments. 3. Nitrogen losses through feces and urine were the highest with $T_1$ (p<0.05) and nitrogen retention rates of groups $T_1$, $T_2$, $T_3$ and $T_4$ were 18.6, 32.4, 35.5 and 27.5% of nitrogen intake, respectively, indicating that RPLys supplementation improved nitrogen retention in sheep.

• 한국유가공학회:학술대회논문집
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• 한국유가공기술과학회 2005년도 창립 30주년 기념 국제심포지움 - 웰빙시대의 우유.유제품의 새로운 발견
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• pp.13-35
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• 2005

노인 인구 증가는 유럽과 미국에 걸쳐 몇 년간 관찰되어 왔지만, 일본과 한국과 같은 개발도상국에서도 증가 추세가 현저히 나타나고 있다. 개발도상국에서는 2000년, 65세 이상 노인의 인구비율이 1960년에 비해 약 5배 정도 증가했으며, 2050년에는 전체 인구의 40% 정도를 차지할 것으로 예상되고 있다. 이런 급격한 인구 변화는 이들이 특정 권리와 구매력을 지니는 새로운 사회경제적 집단으로 성장하게 하는 계기가 되었다. 노화가 일어나면, 식이요법으로 어느 정도 조절할 수 있는 인체의 변화가 다양하게 일어난다. 그 중 대표적인 것은 글루타치온 합성과 이용의 균형에 변화가 생기는 것이다. 글루타치온은 인체에서 가장 중요한 항산화 물질이고, 식이 내 시스테인 아미노산에 의해 체내 함량이 결정될 수 있다. 시스테인이 풍부한 펩티드 제품이 기능성 식품 및 식품원료로 개발되었다. 소비자 조사 결과, 이 제품은 숙면과 활력을 제공하는 등 장점이 있음이 밝혀졌다. 동물 임상 실험 결과를 통해 특별히 노인 인구를 대상으로 하여 시스테인 펩타이드의 생리 활성과 대사 과정을 소개하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.796-804
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• 2004

We found previously that dietary high fat caused obesity, and levan supplementation to the regular diet reduced adiposity and serum lipids. In the present study, we examined the effects of levan [high-molecular-mass $\beta$-(2,6)-linked fructose polymer] supplement on the development of obesity and lipid metabolism in rats fed with high-fat diet. Thus, to determine whether the dietary levan may have the anti-obesity and hypolipidemic effects, 4-wk-old Sprague Dawley male rats were fed with high-fat diet for 6 wk to induce obesity, and subsequently fed with 0, 1, 5, or 10% levan supplemented high-fat diets (w/w) for another 4 wk. For the comparison, a normal control group was fed with AIN-76A diet. Supplementation with levan resulted in a significant reduction of high-fat-induced body weight gain, white fat (i.e., epididymal, visceral, and peritoneal fat) development, adipocyte hypertrophy, and the development of hyperinsulinemia and hyperlipidemia in a dose-dependent manner. Serum triglyceride and free fatty acid levels were greatly reduced by levan supplementation. Serum total cholesterol level was reduced, whereas the HDL cholesterol level was increased by dietary levan. The expression of uncoupling protein (UCP) was increased by dietary high fat, and was further induced by levan supplementation. The mRNA level of UCP1, 2, and 3 in brown adipose tissue (BAT) and UCP3 in skeletal muscle was upregulated in rats fed with dietary levan. In conclusion, upregulated UCP mRNA expression may contribute to suppression of development of obesity through increased energy expenditure. The present results suggest that levan supplementation to the diet is beneficial in suppressing diet-induced obesity and hyperlipidemia.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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• pp.80-82
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• 2001

죽초액을 사료내 0%, 1%, 2% 및 4%로 첨가하여 5주 동안 급여했을때 육성오리의 생산성 및 육질에 미치는 영향을 조사한 결과는 다음과 같다. 증체량과 일당증체량은 죽초액 1%구에서 비교적 증가하였으나 유의성은 인정되지 않았다. 사료섭취량은 죽초액을 첨가함에 따라 약 간 증가하는 경향이였다. 도체중은 1%구가 비교적 증가하는 경향이였다. 죽초액 2%와 4%구에서 육성오리 가슴육의 단백질함량의 유의적인(P<0.05) 증가와 지방의 유의적인(P<0.05) 감소를 나타냈다. 콜레스테롤 함량과 TBA는 죽초액을 첨가함으로써 유의적(P<0.05)으로 감소하였다. 지방산 조성은 죽초액 2%구에서 콜레스테롤의 전구물질이며 포화지방산의 가장 높은 비율을 차지하는 palmitic acid가 죽초액을 급여함으로써 감소하는 경향인 반면에, 맛과 풍미에 영향을 주며 불포화지방산의 가장 높은 비율을 차지하는 oleic acid는 죽초액을 급여함으로써 증가하는 경향이었다. 관능평가 결과 맛과 풍미 그리고 외관에 있어 죽초액 1%와 2%구에서 유의적(P<0.05)으로 높게 나타났다. 장내 살모넬라균과 대장균의 수가 죽초액의 첨가수준의 증가에 따라 감소하는 경향으로 항균작용 효과가 있음을 알 수 있었다.

• 한국가금학회지
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• 제28권1호
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• pp.15-25
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• 2001

This experiment was conducted to investigate the effect of feeding two strains of Lactobacillus and virginiamycin on performance, nutrients digestibility and intestinal microflora of broiler chicks(Abor acres$\times$Abor Acres) were randomly allocated into six treatments with four replications for five weeks. Control(no supplement), 0.05% virginiamycin(VM), Lactobacillus crispatus avibro1(LC), Lactobacillus reuteri avibro2(LR), LC+0.05% VM(LC+VM), LR+0.05% VM(LR+VM) were supplemented into basal diets, which contained ME 3,100kcal/kg and CP 22.0, 20.0% for starting and finishing period, respectively. Weight gain, feed intake and feed conversion(FC) were weekly measured. Nutrients digestibility, intestinal microflora and fecal noxious gas were examined at the end of experiment. Weight gains of chicks fed Lactobacillus or VM was significantly higher than control(P〈0.05). Feed intake increased significantly in those supplemental groups(P〈0.05). FC of chicks fed Lactobacillus or VM significantly lower than control(P〈0.05). Degestibility of crude protein, calcium, and phosphorus improved significantly in alone or combined Lactobacillus treatments(P〈0.05). Whereas DM, crude fat and ash digestibility were not statistically different. Feeding Lactobacilli tended to increase the total Lactobacillus spp. in ileum at one and three weeks of age(WOA) and showed significantly higher in cecum than control at 5 WOA. Total yeast were not shown difference at 1 and 3 WOA, but significantly increased at 5 WOA(P〈0.05). The ileal and cecal anaerobes were started to increase from the first WOA. Fecal NH$_3$gas tended to decrease in Lactobacillus treatments compared to that of other treatments.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.103-110
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• 2020

Objective: The objective of our study was to investigate anti-cancer effects of Danggui Liuhuang Decoction extract bioconverted by protease liquid coenzyme of Aspergillus kawachii (DLD-BE), compared to a non-bioconverted DLD extract (DLD-E) and determine the underlying mechanisms. Methods: DLD-E and DLD-BE were evaluated for their ability to modulate these signaling pathways and suppress the proliferation of human colorectal cancer (CRC) cells, HCT-116, LoVo, and HT-29. The anti-cancer effects of DLD-E and DLD-BE were measured by using proliferation and migration assays, cell cycle analysis, Western blots, and real-time PCR. Results: In this study, treatment with DLD-E and DLD-BE at concentrations of 25-100 ㎍/mL inhibited proliferation and migration in human CRC cells. DLD-BE induced apoptotic cell death and decreased COX-2 expression in HT-29 cells. The mechanisms of action included modulation of the AKT and extracellular-signal-regulated kinase signaling cascades along with inhibition of COX-2 expression. The results demonstrate novel anti-cancer mechanisms of DLD-BE against the growth of human CRC cells. Thus, we propose that DLD-BE can be developed as a more potent supplement to inhibit colorectal tumor growth and intestinal inflammation than DLD-E.

• 한국작물학회지
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• 제47권
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• pp.107-123
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• 2002

This paper is intented to present a information of various aspects of quality related characteristics and standards for grades in maize. Maize is world's one of the three most popular cereal crops and a primary energy supplement and can contribute up to 30, 60, and 98% of the dairy diet's protein, net energy, and starch, respectively. Maize is also processed into industrial goods by wet or dry milling. Sweet corn is a leader among vegetable crops and its production for fresh or processing markets is a major industry in many countries. Over the years, the combined efforts of breeders and geneticists, biochemists, food scientists, and others have helped bring us to the point where we understand issues related to sweet corn quality. Traditional criteria for selecting corn hybrids have been based primarily on agronomic factors, including grain production, disease resistance, drought tolerance, and storage characteristics. Little emphasis has been placed on the quality and nutritional values of corn. Although there is widespread interest for value-enhanced corns have increased tremendously in the last five years, there is limited information available on the production and comparing the quality attributes of specialty grains with those of normal yellow dent corn. Most countries have developed national maize standards, aiming to provide a framework for trade, both internal and external. Where trading involves direct choice and price negotiation in front of the commodity, grading standards are rarely employed; quality is assessed visually and is influenced by end-use, and the price is determined more by local rather than national factors. The use of an agreed standard will provide an unambiguous description of the quality of the consignment and assist in the formation of a legally-binding contract. Standards can also be seen to protect consumers rights through setting limits to the amount of unsuitable or noxious material.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1091-1095
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• 2009

The aim of this study investigated the fluctuations of 3 characters from 3 ecotypes [early ripening (ER), middle ripening (MR), and late ripening (LR)] of 20 Korean brown rice cultivars in different storage systems [time: 12 and 24 weeks, temperature: low ($10^{\circ}C$) and room ($25^{\circ}C$)]. With increase of storage time and temperature, lipoxygenase activity, and fat acidity increased, whereas germination rate was reduced. ER cultivars exhibited the highest lipoxygenase activity of $35.49{\pm}2.46$ unit/mg protein during 24 weeks storage at $25^{\circ}C$, followed by LR ($32.73{\pm}1.33$) and MR ($32.66{\pm}1.62$) cultivars. The amounts of fat acidity also were observed by the same order (ER: $20.40{\pm}2.12$>LR: $19.68{\pm}1.86$>MR: $19.64{\pm}1.35$ mg KOH/100 g). Germination rate slightly decreased with increase of time and temperature (MR>LR>ER), but MR and LR cultivars showed the most significant changes (ER: $60.90{\pm}23.47%$, MR: $32.66{\pm}13.95%$, and LR: $32.53{\pm}5.87%$). On the basis of above results, MR cultivars were evaluated the highest quality, because high lipoxygenase activity, high fat acidity, and low germination rate have deteriorated in quality and generated off-odor. Thus, MR cultivars might be very important sources in food processing and stored dietary supplement aspects.

• Journal of Ginseng Research
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• 제40권3호
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• pp.211-219
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• 2016

Background: Panax ginseng root is used in traditional oriental medicine for human health. Its main active components such as saponins and polysaccharides have been widely evaluated for treating diseases, but secondary active components such as oligosaccharides have been rarely studied. This study aimed to assess the impact of water-soluble ginseng oligosaccharides (WGOS), which were isolated from the warm-water extract of Panax ginseng root, on scopolamine-induced cognitive impairment in mice and its antineuroinflammatory mechanisms. Methods: We investigated the impact of WGOS on scopolamine-induced cognitive impairment in mice by using Morris water maze and novel object recognition task. We also analyzed the impact of WGOS on scopolamine-induced inflammatory response (e.g., the hyperexpression of proinflammatory cytokines IL-$1{\beta}$ and IL-6 and astrocyte activation) by quantitative real-time polymerase chain reaction and glial fibrillary acid protein (GFAP) immunohistochemical staining. Results: WGOS pretreatment protected against scopolamine-induced learning and memory deficits in the Morris water maze and in the novel object recognition task. Furthermore, WGOS pretreatment downregulated scopolamine-induced hyperexpression of proinflammatory cytokines interleukin (IL)-$1{\beta}$ and IL-6 mRNA and astrocyte activation in the hippocampus. These results indicate that WGOS can protect against scopolamine-induced alterations in learning and memory and inflammatory response. Conclusion: Our data suggest that WGOS may be beneficial as a medicine or functional food supplement to treat disorders with cognitive deficits and increased inflammation.