• Title/Summary/Keyword: protein requirement

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Effect of Soil Water Stress on Yield and Quality of Korean Wheat

  • Han-yong Jeong;Yulim Kim;Chuloh Cho;Jinhee Park;Chon-Sik Kang;Jong-Min Ko;Jiyoung Shon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.62-62
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    • 2022
  • Among annual precipitation in Korea (1306.3 mm), 54% of it falls intensively in summer, and only about 12.4% falls in April and May, when the water requirement of wheat is the highest. Korean wheat also could be damaged by soil water excess stress as frozen soil thaws after winter (late Feb-Mar). This study was conducted to evaluate effect of soil water stress on yield and quality of Korean wheat cultivar 'Saegeumgang'. Soil water treatments consisted of 4 treatments; water excess treatment in tilling stage (3.23-3.30), drought treatment in ripening stage (Apr-Jun), irrigation treatment in ripening stage (5.10) and standard condition. There was no significant difference between the treatment conditions for culm length, and the number of spike number was the highest in the order of irrigation in the ripening period (951)> standard cultivation (876)> excess water treatment in the tilling stage (752)> drought treatment in the ripening stage (767/m2). Test weight and Thousand grain weight were 548g/L and 22. lg respectively, which were lower than other treatments, and there was no significant difference between the other treatments. Abortive grain was 5.4kg/10a which was lower than other treatment, and there was no significant difference between the other treatment than other treatments. In drought treatment, protein content was 11.9% which is the highest among all treatments, and SDS-sedimentation value was 27.2ml under drought treatment which was very low compared to other treatments. Therefore, wheat yield and spike number were decreased in excess water condition at tilling stage and drought condition at ripening stage. Furthermore, wheat quality became deteriorate in drought condition at ripening stage.

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Effects of Dietary Levels of Glycine, Threonine and Protein on Threonine Efficiency and Threonine Dehydrogenase Activity in Hepatic Mitochondria of Chicks

  • Lee, C.W.;Cho, I.J.;Lee, Y.J.;Son, Y.S.;Kwak, I.;Ahn, Y.T.;Kim, S.C.;An, W.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.1
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    • pp.69-76
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    • 2014
  • This study was carried out to evaluate the relationship between threonine (Thr) efficiency and Thr dehydrogenase (TDG) activity as an indicator of Thr oxidation on chicks fed with levels of diets (CP [17.5% and 21.5%] and Thr [3.8 and 4.7 g/100 g CP]; glycine [Gly][0.64% and 0.98%] and true digestible Thr [dThr] [0.45% and 0.60%]). Calculation of the Thr efficiency was based on N-balance data and an exponential N-utilization model, and TDG activity was determined as accumulation of aminoacetone and Gly during incubation of hepatic mitochondria. This study found that in the liver of chicks who received a diet containing up to 0.79% Thr (4.7 g Thr/100 g of CP) in the 17.5% CP diet, no significant (p>0.05) effect on TDG activity was observed. However, significantly (p = 0.014) increased TDG activity was observed with a diet containing 21.5% CP (4.7 g Thr/100 g of CP) and the efficiency of Thr utilization showed a significant (p = 0.001) decrease, indicating the end of the Thr limiting range. No significant (p>0.05) effect on the total TDG activity and accumulation of Gly was observed with addition of Gly to a diet containing 0.45% dThr. In addition, addition of Gly to a diet containing 0.60% dThr also did not result in a change in accumulation of Gly. Due to an increase in accumulation of aminoacetone, an elevated effect on total TDG activity was also observed. No significant (p>0.05) reduction in the efficiency of Thr utilization was observed after addition of Gly at the level of 0.45% dThr. However, significantly (p<0.001) reduced efficiency of Thr utilization was observed after addition of Gly at the level of 0.60% dThr. Collectively, we found that TDG was stimulated not only by addition of Thr and protein to the diet, but also by addition of Gly, and efficiency of Thr utilization was favorably affected by addition of Gly at the level near to the optimal Thr concentration. In addition, no metabolic requirement of Gly through the TDG pathway was observed with almost the same accumulation of Gly and a slight increase in TDG activity by addition of Gly. Thus, our findings suggest that determination of TDG activity and parameter of efficiency of Thr utilization may be useful for evaluation of dietary Thr level.

Recommendation of Nitrogen Topdressing Rates at Panicle Initiation Stage of Rice Using Canopy Reflectance

  • Nguyen, Hung T.;Lee, Kyu-Jong;Lee, Byun-Woo
    • Journal of Crop Science and Biotechnology
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    • v.11 no.2
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    • pp.141-150
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    • 2008
  • The response of grain yield(GY) and milled-rice protein content(PC) to crop growth status and nitrogen(N) rates at panicle initiation stage(PIS) is critical information for prescribing topdress N rate at PIS(Npi) for target GY and PC. Three split-split-plot experiments including various N treatments and rice cultivars were conducted in Experimental Farm, Seoul National University, Korea in 2003-2005. Shoot N density(SND, g N in shoot $m^{-2}$) and canopy reflectance were measured before N application at PIS, and GY, PC, and SND were measured at harvest. Data from the first two years(2003-2004) were used for calibrating the predictive models for GY, PC, and SND accumulated from PIS to harvest using SND at PIS and Npi by multiple stepwise regression. After that the calibrated models were used for calculating N requirement at PIS for each of nine plots based on the target PC of 6.8% and the values of SND at PIS that was estimated by canopy reflectance method in the 2005 experiment. The result showed that SND at PIS in combination with Npi were successful to predict GY, PC, and SND from PIS to harvest in the calibration dataset with the coefficients of determination ($R^2$) of 0.87, 0.73, and 0.82 and the relative errors in prediction(REP, %) of 5.5, 4.3, and 21.1%, respectively. In general, the calibrated model equations showed a little lower performance in calculating GY, PC, and SND in the validation dataset(data from 2005) but REP ranging from 3.3% for PC and 13.9% for SND accumulated from PIS to harvest was acceptable. Nitrogen rate prescription treatment(PRT) for the target PC of 6.8% reduced the coefficient of variation in PC from 4.6% in the fixed rate treatment(FRT, 3.6g N $m^{-2}$) to 2.4% in PRT and the average PC of PRT was 6.78%, being very close to the target PC of 6.8%. In addition, PRT increased GY by 42.1 $gm^{-2}$ while Npi increased by 0.63 $gm^{-2}$ compared to the FRT, resulting in high agronomic N-use efficiency of 68.8 kg grain from additional kg N. The high agronomic N-use efficiency might have resulted from the higher response of grain yield to the applied N in the prescribed N rate treatment because N rate was prescribed based on the crop growth and N status of each plot.

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Study on the Soil Improvement in the Grassland II. Effects of the dolomite particles and the shell powder application on soil characteristics, dry matter yield and nutritive value of forages in sandy loam soil (초지에서 토양 개량에서 관한 연구 II. 사양토에서 도로마이트 입자도와 패각분 시용이 토양 특성과 목초의 수량 및 사료가치에 미치는 영향)

  • Lee, J.K.;Choi, S.S.;Kim, M.J.;Yoon, S.H.;Park, G.J.;Choi, K.C.;Lee, S.C.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.4
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    • pp.373-378
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    • 1999
  • This study was conducted to investigate the effects of application of the dolomite particles and the shell powder on soil characteristics, dry matter yield and nutritive value of forage in sandy loam soil at the Experimental Field of National Livestock Research Institute, Suweon, from 1994 to 1996. This was compared with control, lime, dolomite 0.5mm, 2.0mm, 4.0mm, and shell powder in mixed pasture. Although there was no significant difference, average dry matter yield for 3 years was increased with the application of lime, dolomite 2.0mm, 0.5mm, control, 4.0mm and shell powder in order. Average crude protein yield of forages was increased with the application of dolomite 2.0mm, 0.5mm, control, lime, shell powder and 4.0mm in order. Lime requirement was slightly increased from 2,410 to 4,853kg per ha with the lapse of time. Although soil hardness was optimum level by second year, it was apt to become hard little by little. Solid phase of soil was lowered with dolomite 0.5mm treatment. The results demonstrated that dolomite and shell powder could be settled a dust problem in farms and a coast pollution as lime substitutes for soil improvement. Therefore, it is desirable for dry matter yield and crude protein yield to applicate the dolomite 2.0mm every 3 years and the shell powder more frequently in sandy loam soil.

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Nutrient Requirements of Exercising Swamp Buffalo, Bubalus bubalis. II. Details of Work Energy of Cows and Its Relation to Heart Rate

  • Mahardika, I.G.;Sastradipradja, D.;Sutardi, T.;Sumadi, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.7
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    • pp.1003-1009
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    • 2000
  • Four young swamp buffalo cows of similar age ranging in body weight (W) between 280 to 380 kg and trained for doing physical exercise were used in two consecutive experiments, each using a latin square design, to determine energy expenditure for draught. The experiments consisted of field trials using 4 levels of work load, i.e. no work as control and loads amounting 450 to 500 Newton (N) continuous traction for respectively 1, 2 and 3 h daily for 14 consecutive days for experiment 1, and no work, traction loads equaling 5, 10 and 15% of W for 3 h daily for 14 days for experiment 2. Heart rate during rest and exercise was monitored using PE-3000 HR monitor. Cows were fed only king grass (Penisetum purpuroides) ad libitum and were subjected to balance trials. Body composition was estimated in vivo by the body density method and daily energy expenditure (EE) was calculated from ME minus RE. RE was calculated from the changes in body-protein and -fat measured before and immediately after the 14 d experimental period assuming an energy equivalent of 39.32 MJ/kg fat and 20.07 MJ/kg protein. $E_{exercise}$ ($EE_{work}\;-\;EE_{resting}$), which was the energy spent for doing the traction during 1, 2 and 3 h was 7.13, 15.45 and 19.90 MJ, respectively. $EE_{work}$ for the 1 h treatment group was 39.75 MJ/d equivalent to 1.30 times $EE_{resting}$. The values for the 2 and 3 h treatment groups were 1.75 and 1.86 times resting energy requirement, respectively. Absolute efficiency of work in all exercise trials of experiment 2 was around 27.28%. The increases of daily $E_{exercise}$ values were correlated to elevation of heart rate (HR) according to the equation $E_{exercise}=(0.270HR^{0.363}\;-\;1)$ MJ, while draught force related to heart rate according to the equation DF (N)=6.66 HR - 361.62. Blood glucose and triglyceride levels were gradually elevated with time during the course of exercise. Mean values of blood glucose were 91.7, 115.0 and 116.2 mg/dl for cows after 1, 2 and 3 h pulling loads at 15% W respectively as compared to 88.2 mg/dl prior to work. In the same order and treatment, mean blood triglyceride concentrations were 13.5, 13.3 and 14.8 mg/dl, and 11.5 mg/dl for control. For blood lactate, the values were 1.68, 1.63 and 1.66 mM, and 0.80 mM for control. Glucose was used as the major source of energy during the initial phase of exercise, but for prolonged work, fat will replace carbohydrate as the main substrate. Accumulation of lactate persisted for some time at the end of the exercise trials.

Effect of feeding tamarind kernel powder extract residue on digestibility, nitrogen availability and ruminal fermentation in wethers

  • Wang, Lin;Nakanishi, Takashi;Sato, Yoshiaki;Oishi, Kazato;Hirooka, Hiroyuki;Takahashi, Kei;Kumagai, Hajime
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.379-385
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    • 2017
  • Objective: This study was to examine in vivo digestibility, nitrogen balance and ruminal fermentation of tamarind (Tamarind indica) kernel powder extract residue (TKPER) compared to soybean products and by-products in wethers. Methods: Four wethers with initial body weight (BW) of $51.6{\pm}5.5kg$ were assigned in a $4{\times}4$ Latin square design to investigate nutritional characteristics of TKPER, dry heat soybean (SB), dry soybean curd residue (SBCR) and soybean meal (SBM) feeding with ryegrass straw (R) at a ratio of 1:1 at 2% of BW in dry matter (DM) on a daily basis. Results: The digestibility of DM, crude protein, and ether extract (EE) of TKPER-R diet were 57.0%, 87.0%, and 86.0%, respectively. Higher non-fiber carbohydrates digestibility was observed in TKPER-R diet (83.2%) than in SB-R diet (73.9%, p<0.05). Wethers fed the TKPER-R diet had lower retention of nitrogen (N) and ruminal ammonia nitrogen ($NH_3-N$) contents at 4 h after feeding than those fed the SBM-R diet (p<0.05), which had values similar to the SB-R or SBCR-R diet. The TKPER feeding had higher propionate (C3) and lower butyrate content, as well as lower acetate to propionate ratio (C2:C3) in rumen fluid than SBM feeding at 4 h after feeding (p<0.05). Conclusion: TKPER did not bring any side effect to the wethers although it was lack of fiber, and could be used as a high protein and energy ingredient in concentrate with appropriate roughage to meet the fiber requirement for ruminants.

A Study on Food and Nutrient Intakes of Weekday and Weekend Among High School Boys in Seoul (서울 일부지역 남자 고등학생의 주중과 주말의 식품 및 영양소 섭취에 관한 연구)

  • Chai, Hong-Ja;Hong, Hee-Ok;Kim, Hee-Sun;Lee, Jung-Sug;Yu, Choon-Hie
    • Journal of Nutrition and Health
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    • v.41 no.6
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    • pp.539-549
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    • 2008
  • This study was conducted to examine food and nutrient intakes of weekday and weekend with 329 high school boys residing in Seoul using the 3-day food record. Frequency for breakfast skipping was the highest as compared with lunch and dinner skipping. Frequencies for breakfast and lunch skipping were significantly higher in weekend than weekday (p<0.05). The daily average food, vegetable food and animal food intakes were 1,327.6 g, 800.5 g, and 425.8 g, respectively. Total food and vegetable food intakes of weekday were significantly higher than weekend(p<0.05). The dietary variety score(DVS) was 20.5 in daily average, 23.3 in weekday and 15.1 in weekend, which showed significantly higher in weekday than weekend(p<0.05). The daily averages of energy, protein, fat and carbohydrate intake were 2244.9 kcal, 89 g, 72.6 g, and 311.2 g, respectively. Fat intake was significantly lower and carbohydrate, fiber, phosphate, iron, sodium, potassium, vitamin A, niacin, folate, and vitamin C intakes were significantly higher in weekday than weekend (p<0.05). The percentages of energy intake from carbohydrate, protein, and fat were 55.4%, 15.8%, 28.8% in daily average, 56.8%, 15.8%, 27.4% in weekday and 53.6%, 15.8%, 30.7% in weekend, respectively. The percentages of energy intake from carbohydrate in weekday and weekend were below 60%, and that from fat was above 27% in weekday and weekend. Carbohydrate intake was significantly higher and fat intake was significantly lower in weekday than weekend(p<0.05). Energy intakes of daily average, weekday and weekend were above 83% as compared with estimated energy requirement(EER). Intakes of dietary fiber, calcium, potassium, vitamin C, riboflavin and folate were below 75% as compared with adequate intake(AI) or recommended intake(RI). Mean adequacy ratios(MAR), an index of overall dietary quality were 0.78 in daily average, 0.80 in weekday and 0.75 in weekend. MAR of weekend showed significantly lower than weekday(p<0.05). This study revealed that the overall nutrient intake status was worse in weekend than weekday among high school boys.

Nutritional Adequacy of One-dish Meals in the Middle and High School Technology & Home Economics Textbooks Cooking Practice Units (중 고등학교 기술.가정 교과서 조리실습 단원에 게재된 일품요리의 영양 적정성 평가)

  • Kim, Se-Youn;Kim, Young-Nam
    • Journal of Korean Home Economics Education Association
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    • v.19 no.4
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    • pp.175-185
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    • 2007
  • The purpose of this study was to analyze the nutritional adequacy of one-dish meals in the middle and high school technology & Home Economics textbooks cooking practice units. Total of 27 technology & Home Economics textbooks(9 books published by 9 different publishers each for grade 7, 9, and 10) were examined. The total number of one-dish meals introduced in the textbooks was 27, remarkably low, and there are only 7 Korean one-dish meals. It was found that most one-dish meals failed to meet 1/3 of daily estimated energy requirement. According to the analysis of energy contribution ratio by carbohydrate, protein, and fats in one-dish meals, only 4 Korean foods, such as Yubuchobab, Bibimbab, Deokguk and Guksujangguk showed ideal energy contribution ratio. Among the foreign foods, there was no one-dish meal with ideal energy contribution ratio. According to the index of nutritional quality(INQ) analysis, there was no such one-dish meal which supply all the essential nutrients in adequate amounts. All one-dish meals introduced in the textbooks contained excessive protein, but almost no vitamin $B_1$ and $B_2$. Ca and Fe, likely insufficient in Korean people, was found to be a nutrient insufficient also in most one-dish meals.

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Construction and Characterization of a cDNA Library from the Camelina sativa L. as an Alternative Oil-Seed Crop (신 바이오디젤 원료 작물인 Camelina의 cDNA library 제작 및 유전자 특성)

  • Park, Won;Jang, Young-Seok;Ahn, Sung-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.2
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    • pp.151-158
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    • 2010
  • Camelina sativa L., known as popular names "gold-of-pleasure" or "false flax" is an alternative oilseed crop that can be grown under different climatic and soil conditions. Up to date, however, the genomic information of Camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 (55.9%) unigenes were highly homologous to known function genes and the other 375 (44.1%) unigenes were unknown. Remaining 63 (7.4%) unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of Camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were "protein with binding function or cofactor requirement (27%)", "metabolism (11%)", "subcellular localization (11%)", "cellular transport, transport facilities and transport routes (7%)", "energy (6%)", "regulation of metabolism and protein function (6%)". Our result in this study provides an overview of mRNA expression profile and a basal genetic information of Camelina as an oilseed crop.

Effects of different inorganic: organic zinc ratios or combination of low crude protein diet and mixed feed additive in weaned piglet diets

  • Oh, Han Jin;Kim, Myung Hoo;Lee, Ji Hwan;Kim, Yong Ju;An, Jae Woo;Chang, Se Yeon;Go, Young Bin;Song, Dong Cheol;Cho, Hyun Ah;Jo, Min Seok;Kim, Dae Young;Kim, Min Ji;Cho, Sung Bo;Kim, Hyeun Bum;Cho, Jin Ho
    • Journal of Animal Science and Technology
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    • v.64 no.1
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    • pp.23-37
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    • 2022
  • Thirty-six weaned piglets with an initial body weight (BW) of 8.43 ± 0.40 kg (28 days of age, ([Landrace × Yorkshire] × Duroc) were randomly assigned to 6 treatments for a 2-week feeding trial to determine the effects of different inorganic zinc (IZ), organic zinc (OZ) or combination of low crude protein diet (LP) and Mixed feed additive (MFA) on diarrhea score, nutrient digestibility, zinc utilization, blood profiles, organ weight, and fecal microflora in weaned piglet diet. The pigs were individually placed in 45 × 55 × 45 cm stainless steel metabolism cages in an environmentally controlled room (30 ± 1℃). The dietary treatments included a negative control (NC), positive control (PC; zinc oxide, 1,000 mg/kg), T1 (IZ : OZ, 850 : 150), T2 (IZ : OZ 700 : 300), T3 (IZ : OZ, 500 : 500), and T4 (LP + MFA [0.1% Essential oils + 0.08% Protease + 0.02% Xylanase]). The daily feed allowance was adjusted to 2.7 times the maintenance requirement for digestible energy (2.7 × 110 kcal of DE/kg BW0.75). This allowance was divided into two equal parts, and the piglets were fed at 08 : 30 and 17 : 30 each day. Water was provided ad libitum through a drinking nipple. The diarrhea score was significantly increased (p < 0.05) in NC treatment compared with other treatments. The apparent total tract digestibility (ATTD) of dry matter (DM), nitrogen (N), and gross energy (GE) was significantly increased (p < 0.05) in the T2 treatment compared with the PC and NC treatments in week 1. In week 2, the ATTD of DM, N, and GE was significantly decreased (p < 0.05) in the NC treatment compared with other treatments. The T3 treatment had significantly higher (p < 0.05) ATTD and apparent ileal digestibility of zinc than the PC and T1 treatments. The Escherichia coli count in feces was significantly decreased in the T4 treatment compared with the NC and T2 treatments. The Lactobacillus count in feces was significantly increased in the T4 and T1 treatment compared with the T2 and T3 treatments. In conclusion, IZ : OZ 500 : 500 levels could improve nutrient digestibility and zinc utilization in weaned piglets, Moreover, MFA in LP diets could be used as a zinc alternative.