• Journal of Animal Science and Technology
• /
• 제66권3호
• /
• pp.493-503
• /
• 2024

The aim of this experiment was to evaluate the effects of low crude protein (CP) level with essential amino acids (AA) addition on growth performance, nutrient digestibility, microbiota, and volatile fatty acid composition in growing pigs. A total of 160 growing pigs (Landrace × Yorkshire × Duroc [LYD]; average initial body weight 16.68 ± 0.12 kg) were randomly allotted to one of the four treatments on the basis of initial body weight. A randomized complete block design was used to conduct this experiment in the Research Center of Animal Life Sciences at Kangwon National University. There were ten pigs/replicate with four replicates in each treatment. The treatments include; CON (Control, 17.2% dietary CP level), low protein (LP)-1.10 (15.7% dietary CP level + 1.10% lysine level), LP-1.15 (15.7% dietary CP level + 1.15% lysine level), LP1.2 (15.7% dietary CP level + 1.20% lysine level). The pigs fed CON and LP-1.2 diet showed greater final body weight than that of LP-1.1 diet (p < 0.05). Although average daily gain, average daily feed intake, and feed efficiency did not show any difference in phase 2 and 3, average daily gain and feed efficiency was significantly greater in CON and LP-1.20 in phase 1. However, the average daily feed intake did not show any difference during the experimental period. Isobutyric acid and isovaleric acid composition of LP treatments were lower than CON treatment in phase 2. Total branched chain fatty acid composition was significantly lower in LP treatment in phases 1 and 2. However, there was no significant difference among treatments in phase 3. The results of this study underscore the importance of AA supplementation when implementing a low-protein diet during the early growth phase (16-50 kg) in pigs.

• Journal of Nutrition and Health
• /
• 제28권5호
• /
• pp.415-425
• /
• 1995

This study was performed to investigate the effect of dietary protein level on the metabolic changes of Ca and skeletons in postmenopausal women, using ovariecotomized rats as an animal model. The female rats of 200∼250g were fed either 8%(L) or 50%(H) casein diet for 15 weeks(1st experiment). At 15th week, the rats of each diet group were undergone ovariectomy or sham-operation and they were continued to feed the same experimantal diet for 9 more months(2nd experiment). Ca metabolism, kidney function and bone composition were determined at the end of 1st experiment, 3rd and 9th month of 2nd experiment. After 1st experiment, high protein group showed higher urinary Ca and protein excretion, however, there was no difference in GFR and urinary hydroxyproline excretion. The weights, ash and Ca content of femur, scapular and vertebra tended to be higher in high protein groups which tells that high protein promoted skeletal growth. In 2nd experiment, high protein group showed higher urinary Ca and protein excretion and lower Ca absorption and balance. GFR was not affected by dietary protein and ovariectomy but increased with time, as well as kidney weight which shows the continuous development of kidney at this age of 15 month in rats. There were no difference in urinary hydroxyproline, serum ALP, and PTH among experimental groups. The weights of femur, scapular, 4th vertebra increased with time, showing the skeleton continues to grow at this age in rats. However, Ca contents, Ca/wt, Ca/ash were decreased with time and tended to be lower in high protein group especially in femur. In conclusion, prolonged feeding of high protein diet deteriorated Ca metabolism and induced bone loss as time after menopause is extended.

• 한국가금학회지
• /
• 제42권1호
• /
• pp.87-100
• /
• 2015

It is well known that dietary protein affects the growth performance and carcass composition of poultry. Over the last several decades, numerous studies have been carried out to investigate to optimize the level of dietary protein since the protein is an important and expensive constituent in poultry feed. It is generally accepted that dietary protein should represent a balance of amino acids supporting the requirements for growth and maintenance of birds. A protein with balanced essential amino acids that matches a bird's requirement and sufficient non-essential amino acid nitrogen to enable the synthesis of all of the non-essential amino acids, is referred to as an 'ideal protein'. Feeding of excess protein or amino acids may result in an amount of nitrogen emission. Most common method to reduce nitrogen emission is using diet formulation which has lower dietary crude protein level and higher concentration of amino acid supplements. However, there are conflicting reports whether low protein diets supplemented with synthetic amino acids can obtain the growth performance equal to high protein diets. Excessive nitrogen excretion caused by amino acid imbalance also may influence the environment of poultry house due to ammonia production from uric acid. These environmental conditions may increase the incidence of skin problem or respiratory diseases of chickens. Various strategies based on comprehensive understanding should be tested to optimize nitrogen utilization and reduce nitrogen emission while maintaining the performance in poultry production.

• 한국식품영양과학회지
• /
• 제26권5호
• /
• pp.936-942
• /
• 1997

The present study was carried out to investigate the effects of dietary protein and magnesium levels on calcium and magnesium metabolism in male Sprague-Dawley rats. Forty-eight male rats(average weight, 210g) divided into six diet groups; protein 8% and Mg-free diet, protein 8% and Mg 400mg/kg diet, protein 8% and Mg 900mg/kg diet, protein 20% and Mg-free diet, protein 20% nd Mg 400mg/kg diet, and protein 20% and Mg 800mg/kg diet group. After the rats were fed with experimental diets concentration were examined. Kidney weight was significantly higher in protein 8% and Mg-free group compared with other groups. Serum calcium concentration of protein8% group tended to decrease with the increase of the level of magnesium. Serum magnesium concentration in protein 8% and 20% group was lower in Mg-free group than that in other groups(p<0.05) and it also increased as the dietary magnesium level was increased. Urinary calcium and magnesium concentration in Mg-free group were significantly lower than those of other groups.

• 한국가금학회지
• /
• 제14권1호
• /
• pp.1-8
• /
• 1987

사료의 에너지와 단백질 수준이 산란계의 능력에 미치는 영향을 조사하기 위하여 3회의 시험을 실시하였다. 시험1에서는 352일령의 마니나 브라운종을, 시험 2에서는 280일령의 갈색 와렌종을, 시험 3에서는 317일령의 갈색 와렌종을 각각 이용하였다. 각 시험에서 공히 총 360수의 공시계를 18수씩 20개구로 나누어 4개처리에 5반복으로 배치하였다. 시험 설계는 2가지 수준의 에너지(2500과 2800 kcal ME/kg)와 2가지 수준의 단백질 (13% 및 16 %)을 조합한 2$\times$2 요인시험을 실시하였다. 모든 공시계는 산란계용 Cage에 cage 당 2수씩 수용하였으며 시험 1과 2에서는 4주간의 시험기간동안 시험사료와 물을 자유로이 급여하였고 시험3에서는 2주간의 시험기간동안 사료섭취량을 1일 수당 130g으로 제한하여 급여하였다. 시험 1과 2에서 고에너지 사료를 섭취한 닭이 산란율이 다소 높았으나 유의성은 없었다. 그러나 시험 1에서는 단백질 수준이 산란율에 영향을 미치지 못하였다. 사료를 제한 급여한 시험3에서는 고에너지 사료에서 산란율이 개선되었으며(P〈0.05) 단백질 수준도 산란율에 영향을 미쳤으나 유의성은 없었다. 시험 1과 2에서 란중은 에너지 수준이나 단백질 수준에 의하여 영향을 받지 않았으나 시험 3에서는 고에너지 수준에서 란중도 약간 개선되는 경향을 보였다. 3회의 시험 모두에서 사료의 단백질이나 에너지 수준이 증가할 때 egg mass가 증가하는 경향을 보였다. 사료의 에너지나 단백질 수준이 증가할 때 사료섭취량은 감소하고 사료효율이 개선되는 경향을 보였다. 또한 사료의 에너지 수준이 증가할 때 단백질 효율(egg mass/단백질섭취량)이 개선되었으며 사료의 단백질 수준이 증가할 때 에너지 효율(egg mass /ME 섭취량) 이 개선되었다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 춘계학술대회
• /
• pp.93-93
• /
• 2019

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of branches from Taxillus yadoriki parasitic to Neolitsea sericea (TN-NS-B) against human lung cancer cells, A549. TY-NS-B dose-dependently suppressed the growth of A549 cells. TY-NS-B decreased ${\beta}$-catenin protein level, but not mRNA level in A549 cells. The downregulation of ${\beta}$-catenin protein level by TY-NS-B was attenuated in the presence of MG132. Although TY-NS-B phosphorylated ${\beta}$-catenin protein, the inhibition of $GSK3{\beta}$ by LiCl did not blocked the reduction of ${\beta}$-catenin by TY-NS-B. In addition, TY-NS-B decreased ${\beta}$-catenin protein in A549 cells transfected with Flag-tagged wild type ${\beta}$-catenin or Flag-tagged S33/S37/T41 mutant ${\beta}$-catenin construct. Our results suggested that TN-NS-B may downregulate ${\beta}$-catenin protein level independent on GSK3${\beta}$-induced ${\beta}$-catenin phosphorylation. Based on these findings, TY-NS-B may be a potential candidate for the development of chemopreventive or therapeutic agents for human lung cancer.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권1호
• /
• pp.53-60
• /
• 2020

Objective: The current study was carried out to examine the response of different levels of fat and protein in calf starter on nutrient utilisation, nitrogen metabolism, weight gain, blood parameters, and immunity level in pre-ruminant calves. Methods: Twenty four calves (5 days old) were divided into six groups in a 2×3 factorial design, with two levels of fat (10% and 14%) and three levels of protein (18%, 21%, and 24%). The calves were kept in individual pens for 120 days and fed with whole milk (1/10th of body weight) and calf starter ad-libitum. Daily dry matter intake was recorded; whereas body weight was taken on fortnightly basis to calculate average daily gain. During the growth trial blood samples were collected at 30 days interval to estimate blood glucose, albumin, total protein, total leucocyte count, total immunoglobulins and immunoglobulin G levels. A metabolic trial of seven days was carried out to find out the digestibility of different nutrients. Results: The dry matter intake was reduced (p<0.05) with higher fat and protein levels whereas feed conversion efficiency was improved (p<0.05) with higher protein level. Different levels of fat and protein in calf ration did not affect average daily gain in calves. The dry matter, organic matter, and crude protein digestibility were significantly (p<0.01) higher with increased level of protein. The nitrogen retention was also significantly higher (p<0.05) at 24% protein level, similarly the total immunoglobulin was significantly (p<0.05) high in higher protein fed groups, showed better immunity. Conclusion: The present finding suggested that 10% fat and 18% protein level of calf starter could be used in Sahiwal calves for optimum performance in terms of weight gain and immunity.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권12호
• /
• pp.1737-1741
• /
• 2006

The aim of this study was to provide basic information to allow improved nutritional management for velvet production by investigating the effects of dietary protein levels on dry matter intake and production and chemical composition of velvet antler in spotted deer (Cervus nippon). Twenty-four spotted deer stags were assigned to 4 unreplicated groups, Control (15% CP in diet, higher dry matter), CP10 (10% CP), CP15 (15% CP) and CP20 (20% CP). The velvet antlers were harvested from each stag on the 55th day after casting of the buttons from the previous set, measured for their size and weight, and the chemical composition of each antler was determined in three sections (top, middle, and base). Dry matter (DMI) and crude protein (CPI) intake were highest (p<0.05) for the Control and increased progressively (p<0.05) with increasing dietary protein level. Although not significant, mean length and girth of the main antler beam tended to be larger in either left or right beam with increasing protein level in the diet, longest in CP20 and shortest in CP10. Velvet antler production was lowest in CP10 and highest in CP20, which differed significantly (p<0.05). Only negligible differences were found between groups in chemical composition. It is concluded that dietary protein clearly influenced dry matter intake and velvet antler production, whereas there was comparatively little effect of dietary protein on chemical composition of antler in spotted deer.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2011년도 제40회 동계학술대회 초록집
• /
• pp.21-21
• /
• 2011

A series of Quasi-Elastic Neutron Scattering (QENS) experiments helps us to understand the single-particle (hydrogen atom) dynamics of a globular protein and its hydration water and strong coupling between them. We also performed Molecular Dynamics (MD) simulations on a realistic model of the hydrated hen-egg Lysozyme powder having two proteins in the periodic box. We found the existence of a Fragile-to-Strong dynamic Crossover (FSC) phenomenon in hydration water around a protein occurring at TL=$225{\pm}5K$ by analyzing Intermediate Scattering Function (ISF). On lowering of the temperature toward FSC, the structure of hydration water makes a transition from predominantly the High Density Liquid (HDL) form, a more fluid state, to predominantly the Low Density Liquid (LDL) form, a less fluid state, derived from the existence of a liquid?liquid critical point at an elevated pressure. We showed experimentally and confirmed theoretically that this sudden switch in the mobility of the hydration water around a protein triggers the dynamic transition (so-called glass transition) of the protein, at a temperature TD=220 K. Mean Square Displacement (MSD) is the important factor to show that the FSC is the key to the strong coupling between a protein and its hydration water by suggesting TL${\fallingdotseq}$TD. MD simulations with TIP4P force field for water were performed to understand hydration level dependency of the FSC temperature. We added water molecules to increase hydration level of the protein hydration water, from 0.30, 0.45, 0.60 and 1.00 (1.00 is the bulk water). These confirm the existence of the FSC and the hydration level dependence of the FSC temperature: FSC temperature is decreased upon increasing hydration level. We compared the hydration water around Lysozyme, B-DNA and RNA. Similarity among those suggests that the FSC and this coupling be universal for globular proteins, biopolymers.

• Clinical and Experimental Reproductive Medicine
• /
• 제29권1호
• /
• pp.37-44
• /
• 2002

Objective : Heat shock protein family is related to protective mechanism of cells by environmental changes. This study was performed to evaluate the effect of cryopreservation on the heat shock protein 90 (Hsp90) expression in mouse ovarian tissue. Methods : Cryopreservation of mouse ovarian tissue was carried out by slow freezing method. The mRNA level of Hsp90 expression in both fresh and cryopreserved mouse ovarian tissue was analyzed by RT-PCR. The protein expression of Hsp90 was evaluated by Western blot analysis and immunohistochemistry. Results: The mRNA and protein of Hsp90 were expressed in both fresh and cryopreserved mouse ovarian tissue. The amount of Hsp90 mRNA was increased in cryopreserved ovarian tissue after 60 and 90 minutes after thawing and incubation. The amount of Hsp90 protein was increased in the cryopreserved ovarian tissue after 6 hours of the incubation in Western blot analysis. In immunohistochemical study, Hsp90 protein was localized in cytoplasm of oocytes and granulosa cells. Significant level of immunoreactive Hsp90 protein was detected in theca cells contrast to the weak expression in ovarian epithelial cells. Conclusion: This results showed the increase of Hsp90 expression in both mRNA and protein level in the cryopreserved mouse ovarian tissue. It can be suggested that Hsp90 may play a role in the protective or recovery mechanism against the cell damage during cryopreservaion.