• Preventive Nutrition and Food Science
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• 제19권4호
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• pp.327-332
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• 2014

The purpose of the current study was to determine the effects of the introduction of polysaccharide chains onto the molecular surface of buckwheat proteins on buckwheat protein surface functionality. The whole buckwheat protein fraction (WBP) was prepared using 50 mM phosphate buffer (pH 7.5) containing 0.5 M NaCl and covalently linked with 6 kDa, 17.5 kDa, 40 kDa, 70 kDa, or 200 kDa dextran by Maillard-type glycation through controlled dry-heating at $60^{\circ}C$ and 79% relative humidity for two weeks. Conjugation with 40 kDa dextran improved the water solubility and emulsifying properties of WBP without causing a serious loss of available lysine; 84.9% of the free amino groups were conserved. In addition, we found that the introduction of dextran chains onto the molecular surfaces of WBP attenuated the antigenicity of WBP.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.536-541
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• 2017

Hyperglycemia found in diabetes mellitus causes several physiological abnormalities including the formation of advanced glycation end products (AGEs) and oxidative stress. Accumulation of AGEs and elevation of oxidative stress plays major roles in the development of diabetic complications. Adiponectin secreted from adipocytes is known to improve insulin sensitivity and blood glucose level. Curcumin (CCM), a bioactive component of turmeric, has been reported as a potent antioxidant. Present work aimed to elucidate the roles of CCM in high glucose-induced protein glycation and intracellular events in mature adipocytes. The results demonstrated that CCM inhibited the formation of fluorescent AGEs by approximated 52% at 3 weeks of bovine serum albumin (BSA) glycation with glucose. Correspondingly, CCM decreased the levels of fructosamine and ${\alpha}-dicarbonyl$ compounds during BSA glycation with glucose. These data suggested that CCM might be a new promising anti-glycation agent. Also, CCM reduced high glucose-induced oxidative stress in a dose dependent manner, whereas CCM treatment time-dependently elevated the expression of adiponectin gene in 3T3-L1 adipocytes. The findings from this study suggested the possibility of therapeutic use of CCM for the prevention of diabetic complications and obesity-related diseases.

• 한국해양바이오학회지
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• 제13권2호
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• pp.94-103
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• 2021

Here, we evaluated the anti-glycation effects and renal protective properties of 70% (v/v) ethanolic extract of Colpomenia sinuosa (CSE) against AGEs -induced oxidative stress and apoptosis at different concentrations (1, 5, and 20 ㎍/mL). At 20 ㎍/mL, CSE showed that anti-glycation activities via the inhibition of AGE formation (51.1%), inhibition of AGEs-protein cross-linking (61.7%), and breaking of AGEs-protein cross-links (33.3%), were significantly (###p < 0.001 vs. non-treated group) lower than the nontreated group. Methylglyoxal (MGO) significantly (***p < 0.001) reduced cell viability (24.4%) and increased reactive oxygen species (ROS) level (642.3%), MGO accumulation (119.4 ㎍/mL), and apoptosis (55.0%) in mesangial cells compared to the nontreated group. Pretreatment with CSE significantly (###p < 0.001) increased cell viability (57.8%) and decreased intracellular ROS (96.5%), MGO accumulation (80.0 ㎍/mL), and apoptosis (22.6%) at 20 ㎍/mL. Additionally, we confirmed intracellular AGEs reduction by CSE pretreatment. Consequently, our results suggest that CSE is a good source of natural therapeutics for managing diabetic complications by the antiglycation effect and renal protective activity against MGO-induced oxidative stress.

• 한국식품영양과학회지
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• 제39권2호
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• pp.186-192
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• 2010

피부의 주름은 노화과정의 결과로 유도되어지는데, 비효소적 glycation 반응은 주름생성의 원인 중에 하나로 단백질의 비가역적 가교결합에 의해 생성된 최종당화산물(AGEs)이라고 불리는 갈색화합물을 생성한다. 비교적 반감기가 긴 collagen은 쉽게 glycation이 일어나고 collagen의 가교결합(cross-linking)이 일어나면서 여러 단계의 반응을 거쳐 최종적으로 생성된 AGEs가 피부에 축적되어 피부의 탄력감소 및 주름을 일으킨다고 알려져 있다. 피부 내에 glycation된 collagen의 축척은 피부의 탄력성을 유지하는 것을 방해한다. 수종의 식용작물로부터 항주름소재를 개발하기 위하여 메탄올 및 열수추출물을 조제한 후 AGEs 형성을 억제하는 활성을 검색하였다. 먼저 서양향신료 중에서는 월계수잎, 계피, 정향, 오레가노, 로즈마리, 사보리, 팔각과 한약재 중에서는 복분자, 달개비, 삼지구엽초, 가자와 해당화의 메탄올 및 열수추출물에서 최종당화산물 생성 억제활성이 높았다. 그러나 국내 식물 중에서는 단지 참취 및 녹차 추출물의 메탄올과 열수추출물만이 높은 활성을 보였으며 해조류 중에서는 활성이 높은 시료는 없었다.

• 대한화장품학회지
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• 제43권3호
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• pp.239-245
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• 2017

노화는 생리학적으로 비가역적으로 일어나는 과정으로 노화가 진행됨에 따라 단백질의 산화화학반응 등으로 노화징후가 축적된다. 활성산소와 당화된 최종 당화산물(advanced glycation endproducts, AGEs 최종 당화산물)은 생체 조직과 세포를 공격하여 노화를 촉진한다고 알려져 있다. 본 연구에서는 항산화 물질로 알려진 aminoguanidine을 양성 대조군으로 anti-glycation 효과를 확인하였으며, methoxy PEG-45 thioctate(LA-PEG)를 농도별로 처리하여 anti-glycation 효과를 평가하였다. 실험결과 LA-PEG는 매우 우수한 anti-glycation 효과로 최종 당화산물(AGEs) 생성억제 활성이 매우 우수하게 나타났으며, 항산화 효과와 밀접한 연관을 나타냈다. 또, 세포노화 지표물질인 senescence-associated ${\beta}$-galactosidase ($SA-{\beta}-gal$) 활성을 사람 섬유아세포(HDF)를 이용하여 확인한 결과, LA-PEG를 처리하였을 때 염색된 세포의 수가 감소하여 세포의 senescence를 억제하는 것을 확인할 수 있었다. 본 연구결과, LA-PEG의 anti-glycation 효과 및 산화로 인한 단백질 손상에 대한 보호 효과가 우수하게 나타났으며, 항노화 화장품에 적용 시 효과적으로 적용할 수 있을 것으로 사료된다.

• 대한임상약리학회:학술대회논문집
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• 대한임상약리학회 1993년도 정기총회 및 추계학술대회
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• pp.29-29
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• 1993

• The Korean Journal of Physiology and Pharmacology
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• 제22권2호
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• pp.193-201
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• 2018

Connective tissue growth factor (CTGF) is a novel fibrotic mediator, which is considered to mediate fibrosis through extracellular matrix (ECM) synthesis in diabetic cardiovascular complications. Statins have significant immunomodulatory effects and reduce vascular injury. We therefore examined whether fluvastatin has anti-fibrotic effects in vascular smooth muscle cells (VSMCs) and elucidated its putative transduction signals. We show that advanced glycation end products (AGEs) stimulated CTGF mRNA and protein expression in a time-dependent manner. AGE-induced CTGF expression was mediated via ERK1/2, JNK, and Egr-1 pathways, but not p38; consequently, cell proliferation and migration and ECM accumulation were regulated by CTGF signaling pathway. AGE-stimulated VSMC proliferation, migration, and ECM accumulation were blocked by fluvastatin. However, the inhibitory effect of fluvastatin was restored by administration of CTGF recombinant protein. AGE-induced VSMC proliferation was dependent on cell cycle arrest, thereby increasing G1/G0 phase. Fluvastatin repressed cell cycle regulatory genes cyclin D1 and Cdk4 and augmented cyclin-dependent kinase inhibitors p27 and p21 in AGE-induced VSMCs. Taken together, fluvastatin suppressed AGE-induced VSMC proliferation, migration, and ECM accumulation by targeting CTGF signaling mechanism. These findings might be evidence for CTGF as a potential therapeutic target in diabetic vasculature complication.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.383.2-383.2
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• 2002

Diabetic nephropathy is major chronic complication of diabetes mellitus. Advanced glycation endproducts(AGEs) are largely involved in the pathogenesis of diabetic nephropathy. The irreversibly formed AGEs do not return to normal even if hyperglycemia is corrected and continue to accumulate over the lifetime of protein. The AGEs inhibitor. aminoguanidine(AG), is the only protein glycation inhibitor currently under development. its safety however is desirable. To find possible AGEs inhibitor in herbal medicines, bovine serum albumin was added to a mixture of sugars and some of processed. unprocessed herbal medicines or AG. Cyperi rhizoma was processed in four different methods according to chinese pharmacopoeia and traditional literatures. In comparision to the negative control with no inhibitor and positive control with AG. alcoholic extracts of these processed cyperi rhizoma proved to have more potent inhibitory activities than that of unprocessed cyperi rhizoma. These results revealed that some processed herbal medicines have a more potent in vitro inhibitory action on AGEs formation than AG. suggesting the possible candidate for diabetic nephropathy from the processed herbal medicines.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1131-1138
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• 2008

Diabetic complications are a leading cause of blindness, renal failure, and nerve damage. Additionally, diabetes-accelerated atherosclerosis leads to increased risk of myocardial infarction, stroke, and limb amputation. At the present time, 4 main molecular mechanisms have been implicated in hyperglyceamia-mediated vascular damage. In particular, advanced glycation endproducts (AGE), which are formed by complex, heterogeneous, sugar-derived protein modifications, have been implicated as a major pathogenic process for diabetic complications. Recently, AGE inhibitors such as aminoguanidin, ALT-946, and pyridoxamine have been reported. Such an integrating paradigm provides a new conceptual framework for future research on diabetes complications and on discovering drugs to prevent the progression of AGE-induced maladies.

• Fisheries and Aquatic Sciences
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• 제25권10호
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• pp.517-524
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• 2022

Over production of methylglyoxal (MGO) a highly reactive dicarbonyl compound, has been associated in progressive diabetes with vascular complication. Therefore, we investigated whether hot water extract of Loliolus beka meat (LBM-HWE) presents a preserve effect against MGO-induced cellular damage in human umbilical vein endothelial cells (HUVECs). The LBM-HWE extract showed to inhibit MGO-induced cytotoxicity. Additionally, the LBM-HWE reduced mRNA expression of pro-inflammatory cytokines, and reduced MGO-induced advanced glycation end product (AGEs) formation. Furthermore, LBM-HWE induced glyoxalase-1 mRNA expression and reduced MGO-induced carbonyl protein formation in HUVECs. The results implicate that LBM-HWE has protective ability against MGO-induced HUVECs toxicity by preventing AGEs formation. In conclusion, LBM-HWE could be used as a potential treatment material for the prevention of vascular complications of diabetes.