• 제목/요약/키워드: protein film

검색결과 161건 처리시간 0.027초

가소제 및 가교제에 의해 개질된 대두단백질의 특성 (Characteristics of Soybean Protein Resin Modified by Plasticizers and Cross-Linking Agents)

  • 최한나;이태상;양지우;이승구
    • 접착 및 계면
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    • 제12권2호
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    • pp.73-80
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    • 2011
  • 식물성 고분자인 대두단백질을 기반으로 하는 환경친화성 고분자 신소재에 관한 연구를 위해 가소제(1,3-propandiol, glycerol) 및 가교제(glutaraldehyde, epichlorohydrin, glyoxal, urea)에 의한 대두단백질 수지의 열적 특성을 TGA를 이용하여 분석하였고, 기계적 특성 분석과 SEM을 통하여 파단면을 관찰하였다. 그 결과, 가소제인 1,3-propandiol과 glycerol을 SPI (대두단백질)에 첨가함으로써 수지의 유연성이 증가하였고, 1,3-propandiol에 비하여 glycerol의 가소화 효과가 상대적으로 크게 나타났으며, 가교제인 glycerol, epichlorohydrin, glyoxal의 적용으로 첨가량이 증가할수록 대두단백질 수지의 강도와 열안정성이 증가하는 반면, urea의 경우, 대두단백과의 가교가 용이하지 않아 열안정성이 오히려 낮아지고, 강도가 감소함을 알 수 있었다.

한국산(韓國産) 밤의 저장(貯藏)에 관(關)한 연구(硏究) (Studies on the Preservation of Korean Chestnuts)

  • 신두호;배정설;배국웅
    • 한국식품영양과학회지
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    • 제11권3호
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    • pp.41-46
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    • 1982
  • 은기(銀寄) 옥광(玉光) 축파(筑波) 3품종(品種)의 밤의 저장성(貯藏性)을 구명(究明)하기 위하여 움저장(貯藏), polyethylene film 포장저장(包藏貯藏), 상자저장(貯藏), 방사선조사저장(放射線照射貯藏)을 행(行)하여 저장기간(貯藏期間) 동안 화학성분(化學成分) 변화(變化), 부패율(腐敗率), 발아율(發芽率)에 대하여 조사(調査)한 결과(結果)는 다음과 같다. (1) 원료(原料)밤의 화학성분(化學成分)은 수분(水分) 59$\sim$63%, 총당(總糖) 28$\sim$29% 환원당(還元糖) 0.1$\sim$0.2% 조직유(粗織維) 0.6$\sim$0.9% 조단백질(粗蛋白質) 3.5% vitamin C 27$\sim$28mg% 탄닌 50$\sim$58mg%이었다. (2) 총당(總糖)과 vitamin C 함량(含量)은 저장기간(貯藏期間) 동안 감소(減少)하였으며 환당(還糖)과 tannin은 증가(增加)하였다. (3) 발아율(發芽率)은 축파(筑波)가 저장기간(貯藏期間)동안 은기(銀寄)나 옥광(玉光)에 비해 현저히 감소(減少)하였으며 6개월(個月) 저장시(貯藏時) 움저장(貯藏) 93$\sim$100%, 상자저장(貯藏) 35$\sim$57% 방사선조사저장(放射線照射貯藏) 1$\sim$4%였고 polyethylene film 포장저장(包藏貯藏)은 전혀 발아(發芽)되지 않았다.(4) 부패율(腐敗率)은 축파(筑波)가 저장기간(貯藏期間)동안 은기(銀寄)나 옥광(玉光)보다 낮았고 6개월간(個月間) 저장(貯藏)했을 때 상자저장(貯藏) 4$\sim$6%, 방사선조사저장(放射線照射貯藏) 7$\sim$12%, polyethylene film 포장저장(包藏貯藏) 5$\sim$8% 그리고 움저장(貯藏) 30$\sim$54%였다.

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저장 온도와 저장 기간에 따른 꽁치과메기의 일반 성분, 물성 및 미생물학적 변화 (Studies on the General Composition, Rheometric and Microbiological Change of Pacific Saury, Coloabis saira Kwamaegi on the Storage Temperatures and Durations)

  • 이호진;오승희;최경호
    • 한국식품영양학회지
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    • 제21권2호
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    • pp.165-175
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    • 2008
  • Pacific saury, Cololabis saira kwamaegi, is a traditional local food of the Eastern sea area centering around Pohang. It is well-recognized as being both tasty and nutritious. Nevertheless, bacterial contamination, excessive dryness, and compositional changes have made it edible only during the winter months. Therefore, to improve its storage, this study examined the effects of storage material, type, temperature, and duration on compositional changes in kwamaegi. The studied samples were kwamaegis that had been dried naturally for 15 days. The storage materials included an A-film, a self-developed multi-film made of polyethylene, polyamide, EVOH, and polyethylene; as well as a B-film made of polyethylene, nylon, polyethylene, nylon and polyethylene. The B films were used after pressing and laminating. The storage types included one whole fish(1G), or 2 divided fish(2G), to increase eating convenience. The 2G type was the muscle portion divided vertically after discarding the jowl, skin, and internal organs. The storage temperatures were $0^{\circ}C$, $-15^{\circ}C$, and $-30^{\circ}C$, and the storage durations were 2, 4, and 6 months. Pathogenic bacteria and rheology were measured to observe general compositional changes. The whole kwamaegi showed a total cell number of $1,565{\pm}112$ CFU/100 g flesh, while the divided Kwamaegi showed significantly greater bacterial numbers at $2,031{\pm}145$ CFU/100 g flesh. Psychrophils and halophils increased significantly while coliform were not found; the number of mesophils also increased, but not significantly. There were no significant cell number variations between the A-film and B-film. At $0^{\circ}C$, both the A-and B-films resulted in cell numbers of $115{\sim}212$ CFU/100 g flesh, revealing just $7.3{\sim}10.4%$ of the initial storage levels. Overall, there were no significant differences between the storage materials. Generally, as the storage temperature and duration increased, the moisture content of the kwamaegi decreased. Also, as storage duration and temperature increased, crude protein and crude lipid contents increased; in addition, they increased proportionally as the moisture content of the fish decreased. There were no significant differences in crude ash content with respect to the storage materials, storage temperatures, or storage durations. Finally, there were no significant differences between the kwamaegi samples naturally dried for 15 days and those stored in the B-film vacuum storage for 6 months for strength, hardness, cohesiveness, springiness, gumminess, and water activity.

Plasma Surface Modification of Patterned Polyurethane Acrylate (PUA) Film for Biomedical Applications

  • Yun, Young-Shik;Kang, Eun-Hye;Yun, In-Sik;Kim, Yong-Oock;Yeo, Jong-Souk
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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    • pp.223.2-223.2
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    • 2015
  • Polyurethane acrylate (PUA) has been introduced to utilize as a mold material for sub-100 nm lithography as it provides advantages of stiffness for nanostructure formation, short curing time, flexibility for large area replication and transparency for relevant biomedical applications. Due to the ability to fabricate nanostructures on PUA, there have been many efforts to mimic extracellular matrix (ECM) using PUA especially in a field of tissue engineering. It has been demonstrated that PUA is useful for investigating the nanoscale-topographical effects on cell behavior in vitro such as cell attachment, spreading on a substrate, proliferation, and stem cell fate with various types of nanostructures. In this study, we have conducted surface modification of PUA films with micro/nanostructures on their surfaces using plasma treatment. In general, it is widely known that the plasma treated surface increases cell attachment as well as adsorption of ECM materials such as fibronectin, collagen and gelatin. Effect of plasma treatment on PUA especially with surface of micro/nanostructures needs to be understood further for its biomedical applications. We have evaluated the modified PUA film as a culture platform using adipose derived stem cells. Then, the behavior of stem cells and the level of adsorbed protein have been analyzed.

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포도당 산화효소를 고정화한 Polypyrrole 나노튜뷸 효소전극의 포도당 용액 및 당뇨병 혈청에 대한 감응특성 (A Sensing of Glucose Solution and Diabetic Serum using Polypyrrole Nanotubules Enzyme Electrode Immobilized Glucose Oxidase)

  • 김현철;구할본
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 춘계학술대회 논문집 센서 박막재료
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    • pp.6-10
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    • 2001
  • We synthesized polypyrrole (PPy) nanotubules by oxidative polymerization of the pyrrole monomer on the pore of a polycarbonate membrane. The electrochemical behavior was investigated using cyclic voltammetry and AC impedance. The redox potential was about -0.5 V vs. Ag/AgCl reference electrode, while the potential was about 0 V for electro-synthesized PPy film. It is considered as the backbone grows according to the pore wall. Therefore, it is possible to be arranged regularly. That leads to improvement in the electron hopping. The AC impedance plot gave a hint of betterment of mass transport. PPy nanotubules have improved in mass transport, or diffusion. That is because the diffusion occurs through a thin pore wall of PPy nanotubules. The kinetic parameter of PPy nanotubules enzyme electrode with glucose solution was evaluated. The formal Michaelis constant and maximum current calculated by computer were about 23.8 mmol $dm^{-3}$ and $440\;{\mu}A$ respectively. Obviously, an affinity for the substrate and current response of the PPy nanotubules enzyme electrode are rather good, comparing with that of PPy film. What is more, the enzyme electrode is sensitive to blood sugar of a diabetic serum despite an obstruction of ascorbic acid, oxygen, some protein and/or hormone.

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인삼을 첨가한 한과 강정의 산화안정성에 관한 연구 (Oxidative Stability of Korean Traditional Cake added Ginseng)

  • 이정숙;장영상;오만진
    • 한국지역사회생활과학회지
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    • 제17권3호
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    • pp.43-53
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    • 2006
  • In order to improve on the stability during the storage of Han-gwa, this study measured peroxide value, AOM test of ginseng and antioxidants added to Han-gwa. 1.2% of ginseng added to Han-gwa consists of moisture (3.37%), protein(1.30%), lipids (11.54%), carbohydrate(82.45%) and ash (1.34%). The hardness of Han-gwa was 257.7 for 1.2% of ginseng added to Han-gwa and 269.8 for Han-gwa without ginseng. The expansion coefficient of Han-gwa including 1.2% of ginseng was 12.9 magnification to Bandegi, indicating that it was inversely proportioned to the amount of ginseng. The induction period of the AOM test according to the concentration of ginseng in Han-gwa was extended to $0.53{\sim}0.83$ of magnification than the test of Han-gwa without ginseng. According to changes in AOM, acid value, peroxide value and thiobarbituric acid value of Han-gwa with various packing materials, PP multilayer film packing was the most effective material for storage of Han-gwa at 30C. 1.2% of ginseng added to Han-gwa and rosemary extract in a panel test was the most effective, and using PP multilayer film packing for Han-gwa was found to be the most useful method.

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Comparative Kinetic Studies of Two Staphylococcal Lipases Using the Monomolecular Film Technique

  • Sayari, Adel;Verger, Robert;Gargouri, Youssef
    • BMB Reports
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    • 제34권5호
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    • pp.457-462
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    • 2001
  • Using the monomolecular film technique, we compared the interfacial properties of Staphylococcus simulans lipase (SSL) and Staphylococcus aureus lipase (SAL). These two enzymes act specifically on glycerides without any detectable phospholipase activity when using various phospholipids. Our results show that the maximum rate of racemic dicaprin (rac-dicaprin) hydrolysis was displayed at pH 8.5, or 6.5 with Staphylococcus simulans lipase or Staphylococcus aureus lipase, respectively The two enzymes interact strongly with egg-phosphatidyl choline (egg-PC) monomolecular films, evidenced by a critical surface pressure value of around $23\;mN{\cdot}m^{-1}$. In contrast to pancreatic lipases, $\beta$-lactoglobulin, a tensioactive protein, failed to inhibit Staphylococcus simulans lipase and Staphylococcus aureus lipase. A kinetic study on the surface pressure dependency, stereoselectivity, and regioselectivity of Staphylococcus simulans lipase and Staphylococcus aureus lipase was performed using optically pure stereoisomers of diglycerides (1,2-sn-dicaprin and 2,3-sn-dicaprin) and a prochiral isomer (1,3-sn-dicaprin) that were spread as monomolecular films at the air-water interface. Both staphylococcal lipases acted preferentially on distal carboxylic ester groups of the diglyceride isomer (1,3-sn-dicaprin). Furthermore, Staphylococcus simulans lipase was found to be markedly stereoselective for the sn-3 position of the 2,3-sn-dicaprin isomer.

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Effect of γ-Irradiation on the Physicochemical Properties of Zein Films

  • Lee, Sehee;Lee, Myoungsuk;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • 제8권4호
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    • pp.343-348
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    • 2003
  • To elucidate the effect of gamma-irradiation on the physicochemical properties of zein films, the molecular and mechanical properties of the films were examined after irradiation at various irradiation doses. Gamma-irradiation of zein solutions caused the disruption of the ordered structure of the zein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains based on an SDS-PAGE study. Gamma-irradiation increased the solubility of zein and decreased the viscosity due to cleavage of the polypeptide chains. Protein solubility of the zein films in urea/2-mercaptoethanol also increased with increasing irradiation doses. Alterations of the zein molecules by irradiation decreased water vapor per-meability by 12% and increased the elongation of zein films. However, mean tensile strength of the zein films was decreased by gamma-irradiation treatment. Measurement of Hunter color values indicated that irradiation caused a destructive effect on yellow pigments, resulting in a significant decrease in Hunter b values. The microstructure as observed by scanning electron microscopy showed that irradiated zein film had a smoother and glossier surface than the non-irradiated films.

생분해성 고분자, 폴리하이드록시알카노에이트를 이용한 바이오센서 칩 연구와 그 응용 (A Research and Application of Polyhydroxyalkanoates in Biosensor Chip)

  • 박태정;이상엽
    • KSBB Journal
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    • 제22권6호
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    • pp.371-377
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    • 2007
  • 나노기술과 바이오기술의 융합연구에 의해 나노바이오기술이 발전되고 있다. 나노바이오기술의 중요한 응용연구 중의 하나로서, 진단이나 바이오센서 분야에서 단백질-단백질 및 단백질-바이오물질간의 상호작용을 연구하기 위한 단백질 센서 칩이 개발되어 왔다. 본 논문에서는 단백질의 선택적 고정화를 위한 새로운 생체고분자 기질로 PHA를 이용하는 첫 번째 예로서, 단백질-단백질 및 항원-항체 반응의 구현을 나타내고자 하였다. 본 시스템은 PHA 표면 위에서 PHA depolymerase의 SBD와의 선택적 결합에 기반한 것으로, PHA depolymerase의 SBD와 융합된 단백질이 PHA가 코팅된 표면 위에 spotting 될 수 있고 미세접촉인쇄방법에 의해 PHA 위에 미세패턴이 제조되어지는 것을 알 수 있었다(52, 53). 이러한 새로운 전략이 PHA depolymerase의 SBD와 다른 단백질을 융합함으로서 미세 spotting과 미세패터닝이 가능하게 되었고 항원-항체의 생물학적 반응을 통해 많은 바이오센서 칩 연구에 응용될 수 있음을 확인하였다. 또한, PHA 마이크로 비드에도 PHA depolymerase의 SBD와 융합된 단백질을 고정시킴으로서 항원-항체 반응을 유도할 수 있음을 확인하였다(54). PHA의 구조를 변경하여 PHA 기판, PHA 필름, PHA 미세패턴, PHA 마이크로 비드 등을 이용할 수 있으며 multiplex assay를 동시에 진행할 수 있는 다양한 융합 단백질을 사용할 수 있을 것이다. 생분해성 플라스틱으로서 성공적으로 개발된 PHA를 이용한 새로운 플랫폼 기술이 PHA depolymerase의 SBD를 이용함으로서 특이적이고 선택적인 단백질의 고정화에 이용될 수 있음을 확인하였다. 본 전략이 다양한 단백질-단백질 및 단백질-바이오물질 반응을 이용한 바이오칩 및 바이오센서의 응용연구에 유용하게 사용될 것이다.

항균성 미강 단백질 필름 개발을 위한 Bacteriocin 생성균주의 선별 및 특성 (Selection and Characteristics of Bacteriocin-Producing Microorganism to Utilize in Anti-Bacterial Rice Brain Protein Film Production)

  • 김은정;김경미;한혜경;김영호;권기성;배동호
    • Applied Biological Chemistry
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    • 제46권4호
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    • pp.285-290
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    • 2003
  • 경제성과 항균력을 갖춘 미강단백질 필름을 개발하기위한 연구의 일환으로 미강배지에서 bacteriocin을 생성할 수 있는 균주를 선별하고 그 특성을 조사하였다. 그 결과, Pseudomonas aeruginosa 9027에 대한 항균효과를 지닌 Pseudomonas putida 21025를 선별하였으며, 이 균이 생성한 bacteriocin은 쌀에 존재하는 미생물 및 토양미생물에 대하여 넓은 항균활성을 갖는 것으로 나타났다. 지금까지 이용되고 있는 미강단백질 추출 조건에 대한 적합성을 알아보기 위해 bacteriocin의 특성을 조사하였다. Pseudomonas putidia 21025에서 생성되는 bacteriocin은 $50^{\circ}C$이상에서는 한 시간 후 활성이 저하되었으며, 헥산과 에탄올을 제외한 메탄올, 톨루엔, 클로로포름, 아세톤, 메틸 를로로포름등의 유기용매에서 활성이 현저하게 저하되었다. 그러나, 이 bacteriocin은 pH 6.0-9.0에서 2시간동안 안정하였고, $50^{\circ}C$이하에서 안정하였으며, 에탄올에 대하여 3시간동안 안정한 특성을 가지고 있는 것으로 나타났다. 또한 부분정제한 bacteriocin의 분자량은 약 21.6 kDa로 비교적 작은 분자량을 나타내었다.