• Food Science and Preservation
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• v.23 no.4
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• pp.591-598
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• 2016

The purpose of this study was to screen desirable yeast strains for alcoholic fermentation of tomato paste without sugar addition. The moisture, crude protein, crude fat, ash, and soluble nitrogen contents of the tomato paste ($25^{\circ}Brix$) were found to be 67.33%, 1.90%, 0.03%, 0.02%, and 30.72%, respectively. Free sugars found in the paste were fructose and glucose. Most abundant free amino acids of the paste were glutamic, aspartic, and ${\gamma}$-aminobutyric acids. Total seven yeast strains (Saccharomyces cerevisiae KDH (TWA), S. cerevisiae Lalvin ICVD-47 (TWB), S. cerevisiae Lalvin RC-212 (TWC), S. cerevisiae Lalvin K1-V1116 (TWD), S. bayanus Lalvin EC-1118 (TWE), S. cerevisiae Enoferm (TWF), and S. cerevisiae DJ97(KCTC8842P) (TWG)) were tasted for alcohol fermentation of the tomato paste. The highest alcohol content (8.2%) and the lowest residual sugar content ($13.25^{\circ}Brix$) were observed in the tomato paste fermented using the S. cerevisiae Lalvin ICVD-47 strain (TWB) after 3 day and 4 day of fermentation, respectively. Sugar and reducing sugar contents, and pH of the tomato paste were not remarkably affected by the difference in yeast strains used, showing $13.25{\sim}13.45^{\circ}Brix$, 28.37~28.48 mg/mL, and 4.43~4.54, respectively, after 4 day of fermentation. Color and total acid content were significantly affected by the types of yeast strains and fermentation time, but the numerical changes were negligible. These results indicate that TWB would be the suitable strain for alcoholic fermentatiom of tomato paste based on its highest alcohol production and the lowest residual sugar content produced during fermentation.

• Korean Journal of Organic Agriculture
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• v.21 no.1
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• pp.79-93
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• 2013

The experiment was conducted to investigate the effects of feeding herbaceous peat on growth performance and meat quality of Holstein beef cattle. Total of 20 Holstein beef cattle (18~20 month of age, $657{\pm}31kg$ body weight) were conventionally and separately fed a concentrate diet and rice straw for 134 days. The dietary treatments were randomly assigned by complete block design into four treatments, each of which were five heads in early fattening stage. The treatments in this study were the control group fed basal diet, feeding herbaceous peat group (5%/diet, T1), feeding coated vitamin C group (20g/head, T2) and feeding mixture of herbaceous peat and coated vitamin C group (5%/diet+20g/head, T3). The initial body weights between the groups of control, T1, T2 and T3 were similar showing with $689{\pm}31$, $661{\pm}24$, $659{\pm}32$ and $622{\pm}19kg$. The daily body weight gain was higher in T3 by 8.3% than that in the control (p<0.05). Glucose concentration in control group was the highest among treatments (p<0.05), but there was no significant differences between treatments on AST (aspartate aminotransferase), ALT (alanine aminotransferase), BUN and total protein concentrations of blood. The fat content of sirloin in the T2 was significantly higher than control and T1 group (p<0.05). Meat color (CIE) values in T2 was the highest among treatments (p<0.05), and other treatments also increased those values. In overall, the feeding herbaceous peat and vitamin C to the Holstein beef cattle was considered to have positive effects on the growth performance of Holstein beef cattle. In addition, the effects on the performances of animals were more improved when fed herbaceous peat and vitamin C concurrently.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.191-198
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• 1985

Seven different strains of lactic bacteria and 13 combinations of these microorganisms were tested for their acid forming capacity on a vegetable milk made from lupinseed protein concentrate(LPC). L acidophilus, L casei, S. lactis, L. mesenteroides, mixed culture of L. acidophilus and S. thermophilus, and mixed culture of S. lactis and L. mesenteroides were selected and further tested for their growth pattern and acid forming property on lupinseed milk both untreated and partly hydrolized one with carbohydrate decomposing enzymes. The enzyme hydrolized lupinseed milk had 1.5 folds of total free sugar, 8.2 folds of fructose, 3 folds glucose, 2.3 folds maltose, compared to the untreated lupinseed milk. For the untreated lupinseed milk, L. mesenteroides was appeared to be most suitable microorganism having the maximum cell concentration of 1.0 $\times$ 10$^{9}$ $m\ell$ and the final pH 4.40 with the acidity 0.46％. For the enzyme treated lupinseed milk, mixed culture of L. acidophilus and S. thermophilus showed the best performance having 1.9$\times$10$^{9}$ $m\ell$ maximum cell number and the final pH and acidity were 3.69 and 1.13％, respectively. Lactic acid fermentation altered the physical property of lupinseed milk; by fermentation the viscosity generally increased with untreated lupinseed milk, but decreased with enzyme hydrolized one. The viscosity change and sedimentation rate of fermented milk varied with the type of lactic bacteria. The results of sensory evaluation indicated that S. lactis, L. casei, mixed culture of S. lactis and L. mesenteroides, and mixed culture of L. acidophilus and S. thermophilus, grown on enzyme hydrolized lupinseed milk, could produce acceptable lactic beverage.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.812-820
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• 2002

An experiment was conducted on 190 progeny (winter -74; summer -59; rainy -57) of 12 Grey Giant rabbits (10 female +2 males), to assess the effect of different seasons in a year, on their reproductive, growth and productive performances along with feed efficiency, under sub-temperate Himalayan conditions. The daily meteorological attributes recorded during winter (October to March), summer (April to June) and rainy (July to September) seasons, and analysed were minimum and maximum temperature, relative humidity and rainfall. Various biological parameters recorded were doe weights at mating and kindling, litter size at birth, litter weight at birth, kit mortality, litter size at weaning, litter weight at weaning, weekly body weight up to 98 d and weaner mortality. Individual weight gains, dressing percentages, meat weights, liver weights, raw-pelt weights, processed pelt weights and processed pelt areas at slaughter on d 84 and 98, respectively were also recorded. The feed and fodder compositions and their nutritive values during different seasons were also analysed. Average ambient temperature during winter, summer and rainy seasons were $13.2{\pm}2.8$, $22.4{\pm}3.7$ and $24.8{\pm}2.3^{\circ}C$, respectively. The average relative humidity and total rainfall for winter, summer and rainy seasons were $68.9{\pm}1.5$% and $48{\pm}26.6$mm, $66.3{\pm}4.8$% and $125.6{\pm}56.8$ mm, and $77.3{\pm}1.3$% and $116.3{\pm}90.4$ mm, respectively. The weight of doe at mating and kindling, litter size at birth, litter weight at birth and litter size at weaning were comparatively higher whereas litter weight at weaning was significantly (p<0.05) higher during winter as compared to summer and rainy seasons. The kit mortality was significantly (p<0.05) higher during winter while the weaner mortality was significantly (p<0.05) higher during rainy season. At 84 d, the live weight per doe, slaughter weight, dressing percentage and liver weight were significantly (p<0.05) higher during winter than summer and rainy. Similarly, the gain in weight and meat weight at 84 and 98 d were significantly (p<0.05) higher during winter. The weight of raw pelt and processed pelt were recorded significantly (p<0.05) higher during winter while no difference in the area of processed pelts during different seasons could be observed. No difference in the biological performance could be observed between sexes in any of the seasons. Roughage analysis revealed comparatively higher crude protein percent and lower crude fibre percent during summer and rainy seasons than in winter. The roughage dry matter intake was comparatively higher during summer and rainy seasons vis-a-vis constant amount of concentrate supplied during all the three seasons. The digestibilities of dry matter was significantly (p<0.05) lower, whereas that of crude fiber, acid detergent fibre and cellulose were negative during winter. Interestingly, the feed:gain was exceedingly well during winter than in other seasons and it is concluded that it was the best season for production of rabbits under sub-temperate Himalayan conditions.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.757-768
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• 2002

These study were conducted to determine the effects of a whole or steam-flaked corn based diet on rumen microbial fermentation in vitro and ruminal metabolism in the Korean Native Goat(KNG) in vivo. The experiments consisted of two dietary treatments: control, steam-flaked corn(SFC) based diet(80%) + rice straw mixed(20%)(SFCR); 100% whole corn based diet(WC). The first experiment was conducted to investigate the effect of whole corn on ruminal metabolism in vitro for 0 to 48 h. pH values were optimally maintained during incubation time, and were not significantly different between treatments. Gas production of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration tended to increase for WC, but not significantly different between treatments. The mean value of total volatile fatty acid concentration of WC was significantly lower than SFCR(p<0.01), but SFCR and WC linearly increased as the time of incubation approached 48 h. Mean value of acetate concentration of SFCR was significantly higher than WC(p<0.01). Propionate concentration of WC for the total incubation time was significantly higher than SFCR(p<0.01). The digestibility of dry matter was not significantly different between treatments, but SFCR was somewhat higher than WC. The second experiment was conducted to effect of whole shelled corn based diet on rumen metabolism in KNG. pH values tended to decrease through all treatments. There was not a significantly difference between treatments. Microbial protein yield of SFCR was significantly higher than WC(p<0.01). $NH_3$-N concentration of WC was significantly (p<0.01) higher than SFCR. Total VFA and propionate concentration of WC was significantly higher than SFCR(p<0.01), but acetate concentrate of WC was not significantly higher than SFCR. The mean value of total lactate concentration was significantly(p<0.01) different but the value of SFCR and WC were lower than the average concentration of acidosis. In sacco DM disappearance rate of SFC was significantly(p<0.01) higher than WC.

• Journal of Animal Science and Technology
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• v.53 no.4
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• pp.367-375
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• 2011

This study was carried out to investigate the effects of the level of cracked whole barley on daily feed intake, daily body weight gain, carcass characteristics and meat composition of finishing Hanwoo steers (feeding from 24 months to 30 months of age). The dietary treatments were consisted of five types (C; normal concentrate as a basal diet, T1; 10% addition of cracked whole barley, T2; 20% addition of cracked whole barley, T3; 30% addition of cracked whole barley, T4; 40% addition of cracked whole barley). A total 30 Hanwoo steers (588.6${\pm}$11.8kg) were allocated to 5 feeding groups. The daily feed intake and daily body weight gain were high in the order of T2 > T3 >T4 > T1 > C. The back fat thickness and longissmus muscle area were highest in C and T1, respectively (P<0.05) than other treatments. The meat yield index decreased with increased back fat thickness. The marbling score and meat quality were highest in T1 (P<0.01, 0.05, respectively), but maturity, fat color and meat color were not significantly different among treatments. The crude fat was highest in T1 (17.59%), while in T4 (7.47%) it was lowest (P<0.05). The crude protein and crude ash were not significantly different among treatments. The energy value of cracked whole barley treatments (T1, T2, T3, T4) was higher than C (P<0.05). The contents of Ca, Cu, K, Mg, Mo, Na and Zn were higher in C than the other treatments (P<0.01), but Co was higher in T2 (P<0.01). The CIE $L^*$ value of whole cracked barley treatments (T1, T2, T3, T4) was higher than C, however there were not differences among the treatments. The CIE $a^*$ value was highest in T1(P<0.05) than others. The CIE $b^*$ value was highest in C and it was decreased with increased feeding of cracked whole barley. Based on the above results, T1 treatment compared to other treatments improved the carcass quality parameters like loin muscle area, marbling score, meat quality, and CIE $L^*$ value.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.193-200
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• 2004

This experiment was carried out to determine energy requirements for maintenance of Hanwoo steers. Nine Hanwoo steers weighing 376.6$\pm$12.5kg were used in this experiment and fed rice straw(44%) and concentrate (56%) at three different energy levels; 0.8 times maintenance(0.8M), 1.2 times(1.2M) and 1.6 times(1.6M), respectively. Dry matter intake was 48.5, 65.9 and 86.5g/$BW^{0.75}$ for 0.8M, 1.2M and 1.6M, respectively. Increase in energy intake with the increased DM intake did not affect digestibilities of dry matter, organic matter, crude protein, crude fiber, crude fat and nitrogen-free extract. Gross energy intake averaged 190.8, 255.8 and 340.9kcal/BW0.75 for 0.8M, 1.2M and 1.6M, respectively. Energy loss was 41% feces and 0.6${\sim}$1.5% urine of gross energy intake. Further, energy loss from methane produced during rumen fermentation was 5${\sim}$9%, while body heat loss averaged 40${\sim}$60%. Intercept of the regression equation between ME intake and retained energy indicated that energy requirement for maintenance was 124.3kcal $ME/BW^{0.75}$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.2
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• pp.61-67
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• 2019

This study was conducted to investigate the effect of supplementary feeding levels on livestck and forage productivity and grazing intensity in Elk stags (Cervus canadensis). A fifteen 2-year-old Elk stags about 195 kg were randomly assigned to one of three dietary treatments (five animals per treatment). The dietary treatments consisted of a feeding concentrate of 1.0% of body weight (T1), 1.5% of body weight (T2) and 2.0% of body weight. Total dry matter intake (TDMI) was increased with increased with an increasing supplementary feeding levels. Average daily gain (ADG) were significantly increased with an increasing supplementary feeding levels (p<0.05) and reached a maximum on July and was lower in spring than autumn. The velvet antler production was no differences among treatment groups. Forage productivity of pasture and crude protein content were highest on May and decreased thereafter, however, crude fiber content was the reversed. The grazing intensity of Elk stags was increased in spring (38 to 59 head per ha) than summer and autumn (13 to 32 head per ha). The average grazing intensity of Elk stags ranged from 21 to 34 head per ha, which is affected by supplementary feeding levels. This result suggests that feeding supplementary diet at 1.5 % of body weight was needed to maintain the stable wight gain in antler growing periods and control the proper grazing intensity of Elk deer stags.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.2
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• pp.102-109
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• 2021

In this study, the effect of forage sources in the total mixed ration (TMR) on in vitro goat rumen fermentation was investigated. Rice straw (RS), Italian ryegrass (IRG), timothy (TIM), and alfalfa (ALF) were used as forage sources. Each forage source was mixed with a commercial goat concentrate diet in the ratio of 1:1. Total 4 TMR were prepared. Rumen simulated in vitro fermentation using goat rumen fluid collected from the slaughterhouse was conducted until 72^th. For fermentation parameters, gas production (GP), volatile fatty acids (VFAs), and ammonia nitrogen (NH₃-N) were examined. All assays were performed at 24^th, 48^th, and 72^th h of incubation individually. Contents of crude protein and non-fibrous carbohydrate were greater in the order of RS < IRG < TIM < ALF. Significant treatment effects were found in valerate and NH₃-N at 24^th h of incubation (p<0.05). ALF showed the greatest contents of them and RS was the lowest. At 48^th incubation, a significant effect was detected at GP (p<0.05) and RS was greater than others. However, GP of RS was lower than others at 72^th. Significant effects on Total VFA, butyrate, and valerate productions were found at 72^th h of incubation (p<0.05). ALF showed the greatest production. Methane production from all treatments was not significantly different for each incubation time (p>0.05). The present study provided primary information on how goat rumen fermentation responds to different nutrient contents and forage sources of TMR. And the information could be used for the design or optimizing economical diet formulation for goats.