• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.455-462
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• 1995

Arbor Acres broiler chickens (N=288) with an average initial weight of 59.4 g were fed diets varying in protein and lysine (80, 100, 120% of NRC; 100, 120% of NRC, 1984) in order to investigate the effects of supplemental chromium picolinate on growth performance, nutrient utilizability, carcass composition, serum traits and in vitro protein synthesis. Six replicates of eight chicks were grouped into one treatment Six chicks were sacrificed from each treatment for carcass analysis, and six additional chicks were chosen and dissected for in vitro culture of liver tissue. Body weight gain, feed intake, feed conversion, mortality, carcass composition and serum glucose, HDL/cholesterol ratio, serum triglyceride and serum nonesterified fatty acid appeared to be affected by either the level of dietary crude protein or lysine when supplemented with 200 ppb chromium picolinate (p < 0.05). Retained and secreted proteins in liver acinar cell cultured in vitro were not affected by dietary lysine level but affected by dietary protein level when added with 200 ppb chromium picolinate.

• BMB Reports
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• v.43 no.10
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• pp.670-676
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• 2010

In this paper, a novel approach, ELM-PCA, is introduced for the first time to predict protein subcellular localization. Firstly, Protein Samples are represented by the pseudo amino acid composition (PseAAC). Secondly, the principal component analysis (PCA) is employed to extract essential features. Finally, the Elman Recurrent Neural Network (RNN) is used as a classifier to identify the protein sequences. The results demonstrate that the proposed approach is effective and practical.

• Journal of Environmental Science International
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• v.30 no.7
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• pp.537-545
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• 2021

This study aimed to investigate the improvement effect of 5% (w/w) mung bean (Phaseolus aureus L.) on lipid composition and protein concentration in hyperlipidemic rats. Seven-week-old male (Sprague-Dawley) rats were divided into four groups (n=6, each), and fed experimental diets containing mung bean meal [basal diet+5% (w/w) mung bean (BM), basal-hyperlipidemic diet+5% (w/w) mung bean (BHM)], basal-hyperlipidemic diet (BH), and control (basal diet, BD). Lipid composition in the mung bean diet groups (BM and BHM) evidenced significant reductions in serum total cholesterol, low-density lipoprotein cholesterol, atherosclerotic index, cardiac risk factor, triglyceride, phospholipid, free cholesterol, cholesteryl ester, blood glucose, nonesterified fatty acid, and an elevation in high-density lipoprotein cholesterol. The serum albumin/globulin ratio was increased in mung bean diet-supplemented rats compared to that in hyperlipidemic rats (p < 0.05). Total calcium, phosphorus and potassium concentrations in sera were higher in the BM, BHM and BD groups than in the BH group. Concentrations of sodium and chlorine in sera were lower in the mung bean diet-supplemented groups than in the hyperlipidemic group. The in vivo experiments showed that ingestion of mung bean was effective in improving lipid composition, protein concentration, and blood glucose.

• Journal of Aquaculture
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• v.9 no.2
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• pp.167-168
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• 1996

Experiments were conducted to determine the activities of protease and carbohydrase in growing lensky sturgeon fed with three different diets containing various concentrations of protein and carbohydrate. Neutral pretense activity from growing lensky sturgeon the protein diet (predo-minary, almost $100\%$ protein) was lower then those from fish fed the other diets during the experimental period. The results may indicate that the level of pretense activity is inversely related to the level of protein in the diets.

• Journal of Nutrition and Health
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• v.25 no.6
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• pp.485-491
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• 1992

The effects of varying doses(1, 4 and 10mg/kg body weight/day) of testosterone propionate (TP) on body weight gain and composition and energy and muscle protein metabolism were investigated in female rats. TP had no effect on food intake at any dose but injection of 1mg/kg resulted in an in crease in body weight gain which was associated with increases in body protein and fat. At higher doses(4 and 10mg/kg) body protein content was still increased but body fat was not affected. Increases in energy gain and gross energetic efficiency were observed at a dose of 1mg/kg but neither parameter was affected at other doses. The mass protein and RNA content of gastrocnemius muscle were incerased by TPbut the ratio of RNA to protein and the rate of muscle protein synthesis measured in vivo were not affected at any dose of TP The results indicate that the effect of testosterne on body composition are highly dose-dependent and the anabolic action of testosterone is not through stimulation of protein synthesis.

• Korean Journal of Food Science and Technology
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• v.10 no.1
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• pp.83-87
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• 1978

Dehulled pumpkin seeds produced in Korea were defatted and their composition of fatty acid was analyzed by gas chromatography. Soluble protein and insoluble protein were isolated from the dehulled and defatted pumpkin seeds. The protein component was identified by disc-electrophoresis and the amino acid composition of the protein was analyzed. (1) The pumpkin seed contained 46.5% of fat and 25.5% of protein respectively. (2) The fatty acid of the pumpkin seed was compassed chiefly of linoleic acid (51.74%), oleic acid (22.2%), palmitic acid (18.1%) and stearic acid (7.84%) (3) The amino acid composition of the soluble and insoluble seed protein contained most of all the amino acids except for S-contained amino acids. (4) It has been identified by disc-electrophoresis that the soluble protein had 13 bands and the insoluble protein had 4 bands.

• Journal of Nutrition and Health
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• v.19 no.1
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• pp.66-73
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• 1986

This study was undertaken to investigate the effects of age and of the dietary protein sources & levels on urinary calcium excretion and bone metabolism in the rats. Two experiments were conducted . In experiment U, 6& 20 weeks old rats were fed 8 & 36%, casein & ffish protein diet for 5 weeks . In experiment II, 16& 52 weeks old rats were fed 40 % protein diets, protein sources were casein, fish and gluten. High-protein diet group excreted more caldium in urine than low-protein group. Urinary calcium excretion was affected by the sources of protein ; gluten group excreted more Ca, followed by fish & casein group. Total bone Ca & Ca proportion in ash were higher in 20 weeks old rats than 6 weeks old rats, but 16 & 52 weeks old rats showed no differences. bone composition showed that water proportion was high and ash proportion was low in 6 weeks old rats than in 20 weeks old rats. However, these tendencies were not observed between 16 weeks and 52 weeks old rats. And bone composition was affected by protein sources ; higher ash proportions were noted in one order of casein, fish, and gluten water proportion were lower in one same order.

• Journal of Life Science
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• v.8 no.3
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• pp.318-325
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• 1998

To investigate changes in protein and total lipid contents, seed storage protein pattern, and fatty acid composition of germination perilla(Perilla frutescens) seeds. Also, the corresponding value components in cotyledons, hypocotyles and roots were measured according to germination stage. The results were summarized as follows ; During germination, pertein and total lipid contents of Yepsilldalggae and Kwangyang cultivar were decreased continuously. In particular, protein contents rapidly decreased to the 3 days after germination(DAG), and then total lipid contents rapidly decreased between 3 DAG and 10 DAG. In changes of protein and total lipid contents of cotyledons, protein contents of Yeupsildalggae was increased during the germination, but Kwangyang cultivar was decreased during the same periods. The total lipids contents of Yeupsildalggae and Kwangyang cultivar were decreased during the germination. According to SDS-PAGE analysis, there was no detectible polypeptide bands on the gel before seed germination suggesting that this may be due to the rapid degradation of the storage proteins in the mature seed by hydrolyttic enzymes during the stage. During germinatation , the polypeptide band with 27$\sim$28KD of Yeupsildalggae and Kwangyang cultivar were accumulated gradually. In changes of fatty acid composition of total lipid of Yeupsildalggae and Kwangyang cultivar , saturated fatty acids such as palmitic acid and stearic acid increased during the germination. On the other hand, unsaturated fatty acid such as linoleic acid and linolenic acid decreased during the same periods. However, oleic acid increased to the 5 DAG, and then was repidly decreased.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.5
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• pp.259-268
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• 2007

To measure the proximate composition of fisheries products and to evaluate their nutritional value, we collected individuals from 101 species from the east (Pohang), west (Gunsan), and south (Tongyeong) coasts of Korea: 51 fish species, 32 species of molluscan shellfish (Gastropoda and Bivalvia), 6 species of Cephalopoda, 8 species of Crustacea, and 4 other species. The proximate composition of fish was $75.3{\pm}5.0%\;moisture,\;19.1{\pm}2.7%\;protein,\;3.8{\pm}4.1%\;lipids,\;0.4{\pm}0.3%$ carbohydrates, and $1.4{\pm}0.3%$ ash. The proximate composition of molluscan shellfish was $79.1{\pm}3.2%\;moisture,\;14.2{\pm}3.2%\;protein,\;0.7{\pm}0.6%\;lipids,\;3.9{\pm}1.9%$ carbohydrates, and $2.1{\pm}0.4%$ ash. We observed clear regional variation in the lipid content of some fish species. Specifically, the lipid content of gizzard shad (Clupanodon punctatus) was highest in the autumn, while the lipid contents of red seabream (Pagrus major) and purple pike conger (Muraenesox cinereus) were highest in the winter. The daily average intake of the proximate composition through fisheries products consisted of 11.7 g of protein, 1.9 g of lipids, 1.1 g of carbohydrates, and 1.1 g of ash. The respective intakes of protein, lipids, and carbohydrates from fisheries products were about 19.3, 3.6, and 0.3% of the nutrient reference values set by the Korean Food & Drug Administration. Therefore, fisheries products playa very important role as a source of protein.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.957-967
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• 2016

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.