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Effects of Protein and Fiber on Antioxidant Enzyme Activites of Brain in Ethanol-Treated Rats (에탄올을 투여한 흰쥐 노조직의 항산화효소계 활성에 미치는 단백질과 섬유소의 영향)

  • 이미경
    • Journal of Nutrition and Health
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    • v.33 no.6
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    • pp.613-618
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    • 2000
  • This study was to investigate the effect of dietary protein and fiber on the antioxidant enzyme activities of brain in acute or chronic ethanol-treated rats. Male Sprague-Dawley rats were fed on diets containing two levels of protein(7%, 20%) with two levels of fiber (5%, 10%) Rats were administered 40%(v/v) ethanol(5g/kg body weight)orally 90min before decaptiation in acute ethanol-treated groups and 25%(v/v) ethanol(5g/kg body weight) once a day for 5 weeks in chronic ethanol treated-groups. The rats were sacrificed after 5 weeks of feeding periods. Superoxide dismutase and gluthathione S-transferase activities were lower in chronic ethanol-treated groups than acute ethanol-treated groups whereas catalase and glutathuone peroxidase activities were significantly increased by chronic ethanol treatment. Low protein supplement accelerated to change of their activities however dietary fiber levels did not affect antioxidant enzyme activities. Chronic ethanol treatment and/or low protein supplement results in increasing the brain lipid peroxide content but in lowering glutathione level. (Korean J Nutrition 33(6) ; 613~618, 2000)

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Effects of Protein Intake on Sarcopenia Prevention and Physical Function of the Elderly in a Rural Community of South Korea During the COVID-19 Pandemic (COVID-19시대 한국 농촌 지역 노인의 단백질 섭취가 근감소증 예방 및 신체기능에 미치는 영향)

  • Li, Xinxing;Kim, Hee-Jae;Kim, Dae-Young;Zhang, Yanjie;Seo, Ji-Won;Ahn, Seo-Hyun;Song, Wook
    • Journal of The Korean Society of Integrative Medicine
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    • v.10 no.2
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    • pp.37-47
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    • 2022
  • Purpose : We aimed to investigate the effects of nutritional foods on sarcopenia prevention and physical function among the elderly living in rural communities during the COVID-19 pandemic by providing customized nutrition. Methods : This study was conducted in the rural community of SCC. Participants (n=24, over age 65) were randomly assigned into a Protein group (n=12) and a Vitamin group (n=12). The protein group was given 23 g/d of protein (whey, soybean, BCAA) for 8 weeks and the Vitamin group 23 g/d of vitamin (B, C, D, E and mixed minerals such as calcium, magnesium, zinc) for 8 weeks. All participants had their body composition such as height, weight, skeletal muscle mass, body mass index, and body fat percentage, measured using bioelectrical impedance analysis (BIA) and physical function assessed using grip strength and the short physical performance battery (SPPB). Results : At the end of the intervention, there was a significant increase in skeletal muscle mass (p<.01) in the Protein group (p=.002, 4.92 %) compared to the baseline: it increased by 2.33 %. The Vitamin group had a significant increase in body fat percentage after the intervention (p=.001, 15.35 %) compared to the baseline: body fat percentage decreased by 4.49 %. There were no significant differences in left and right Grip strength/Weight, SPPB, 4-m gait speed, chair stand test, and sense of balance in both groups. Conclusion : The findings from this study suggest that 8 weeks of protein intake have a significant effect on skeletal muscle mass and body fat percentage. Protein intake helped promoting the health of the elderly in rural community during the COVID-19 pandemic. It will assist creating a foundation for providing customized nutrition for the elderly in rural community in the future.

Optimum Protein Levels in Diet for Fingerling Korean Catfish, Parasilurus asotus (메기, Parasilurus asotus 사료의 최적 단백질 함량)

  • KANG Seok-Joong;JEONG Woo-Geon
    • Journal of Aquaculture
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    • v.6 no.1
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    • pp.47-53
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    • 1993
  • Feeding trials were conducted for a test of the optimum dietary protein levels for the fingerling Korean catfish, Parasilurus asotus. The growth response was examined in terms of weight gain, feed coefficient. protein efficiency ratio and net protein utilization for 8 weeks at $23\~27^{\circ}C$. Within a range of 25 to 55\%$ crude protein levels in the diet, the body weight increased while the dietary protein level increased. Accumulation of protein in the body reached the maximum when the crude protein level in the diet was at $45\%$. These results indicate that the optimum dietary protein level of fingerling Korean catfish was about $45\%$ when anchovy meal was used as the protein source.

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Protein, Ca, Mg and P Intakes of Breast-fed Infants during Lactation (모유영양아의 수유기간별 단백질, 칼슘, 마그네슘과 인 섭취량)

  • 김을상;금혜경
    • Journal of Nutrition and Health
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    • v.36 no.9
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    • pp.942-949
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    • 2003
  • This study was longitudinally conducted to evaluate the intakes of protein, Ca, Mg and P of exclusively breast-fed infants compared with the Recommended Dietary Allowances (RDA) for Korean infants. Twenty Korean lactating women and their infants during the first 3 months of lactation in Incheon area were participated. Protein, Ca and Mg, and P contents in the milk were determined using semimicro Kjeldahl (N ${\times}$ 6.38) , atomic absorption spectrophotometer and colorimeter, respectively, and also the milk consumption of the infants was measured by the test-weighing method. Protein contents of the milk were 1.96, 1.63, 1.51, 1.25 and 1.16 g/100 ml, and protein intakes of the breast-fed infants were 9.00, 9.85, 9.17, 8.97 and 7.76 g/day at 7, 15, 30, 60 and 90 days postpartum. The average protein intake per body weight of the breast-fed infants was 1.84 g/kg/day. The average intakes of Ca, Mg, P were 172.1 mg/day, 15.2 mg/day and 91.4 mg/day, respectively, and the average Ca/P ratio was 1.91. There was positive correlation between protein and Ca, protein and p, and Ca and P contents while negative correlation between Mg and P, The body weight of breast-fed infants increased normally from 3.6 $\pm$ 0.41 g at birth to three month during lactation. It is suggested that the breast-fed infants in Incheon area consume almost adequately protein, Ca and P from the milk compared with RDA for Korean infants.

The Effect of Dietary Protein and Calcium Levels on Metallothionein and Histopathological Changes during Cadmium Intoxication in Rats (식이 단백질과 Ca 수준이 흰쥐의 Cd 중독과정중 Metallothionein 과 조직의 형태변화에 미치는 영향)

  • 권오란
    • Journal of Nutrition and Health
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    • v.25 no.5
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    • pp.360-378
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    • 1992
  • This study was performed to investigate effect of dietary protein and calcium levels on cad-mium intoxication in rats. Adult Sprague-Dawley male rate(245$\pm$21g) were blocked into 18 groups of 7 animals according to body weight Nine experimental diets different with protein(40%, 15%, 7%) and calcium (1.3%, 0.6%, 0.1%) levels were prepared. Nine groups of animals were fed each diet with 50ppm cadmium in drinking water and the other 9 groups without cadmium for 30days. Results were summarized as follows: 1) Body weight gain F. E. R(Food Efficiency Ratio) and weights of liver kidney and femur were higher in high protein groups among cadmium exposed groups. 2) Cadmium contents in liver and intestine were higher in rats fed high protein diet or low calcium diet among cadmium exposed groups. Fecal cadmium excretion was highest in high protein-high calcium diet group among cadmium exposed animals. Metallothionein contents in liver kidney and intestine were higher in animals exposed to cadmium and fed high protein diets. 3) Gel filtration chromatography of cytosolic solution showed that the higher dietary protein and calcium levels were the more cadmium was found in metallothionein fractions. 4) No gross histopathological change was seen in liver kidney and intestine of cadmium exposed rats. However a significant increase of smooth endoplasmic reticulum which was alleveated by high protein-high calcium diet was observed. Results obtained indicated that not only high protein diet but also high calcium diet showed preventive effect on cadmium intoxication by increasing the induction of metallothionein syn-thesis and decreasing the cadmium absorption.

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Effects of the Source of the Dietary Protein on Serum Lipids and Glucose in Streptozotocin-diabetic Rats (식이 단백질의 급원에 따른 당뇨쥐의 혈당 및 혈청지질에 미치는 영향)

  • Lee, Su-Jin;Kim, Young-Jin;Park, Su-Jeong;Kim, Hui-Jung;Lee, Yun-Hui;Yoo, Young-Sang
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.2
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    • pp.153-158
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    • 1997
  • This study was designed to find out the effects of soybean protein and milk protein between nondiabetic and diabetic rats. The experimental results are summarized as follows. 1. Total food intake was higher in diabetic soybean protein group than other groups but it was not significant. 2. The change of body weight was lower in diabetic soybean protein group than other groups and the soybean protein was effective to maintain the ideal body weight. 3. The effects of lowering total cholesterol and glucose in serum was higher in soybean protein groups than the milk protein groups.

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Influence of the Mineral Potassium Permanganate on the Biochemical Constituents in the Fat Body and Haemolymph of the Silkworm, Bombyx mori L.

  • Bhattacharya, A.;Kaliwal, B.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.131-135
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    • 2004
  • Oral supplementation with potassium permanganate (30, 50 and 100 $\mu\textrm{g}$) to fifth instar larvae of the ${CSR_2}{\times}{CSR_4}$ race of the silkworm, B. mori resulted in a significant increase in the glycogen content of the fat body and haemolymph trehalose. The protein content of the fat body is also significantly increased in all the potassium permanganate treated groups where as that of the haemolymph is significantly increased only in the 30 ${\mu}g4 fed group. The total lipids content of the fat body increased significantly in all the potassium permanganate treated groups. This indicates that the potassium permanganate may stimulate metabolic activity, there by influencing the biochemical contents in the fat body and haemolymph of the silkworm, B. mori.

Age-Related Changes in Body Growth, Muscle Protein Content and Cellular-Mediated Immunity and Rats Fed Stock Diets (고형사료로 12개월간 사육한 흰쥐의 월령에 따른 성장과 근육단백질 및 세포매개성 면역능력의 변화)

  • 원혜숙
    • The Korean Journal of Food And Nutrition
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    • v.4 no.2
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    • pp.133-140
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    • 1991
  • This study was designed to investigate age-related changes in body composition and cell mediated immunity. Male Sprague-Dawley rats were fed stock diet ad libitum and 12 rats were sacrified at 1, 4, 6, 12 months of age. Body weight Increased sharply from 1 to 4 months. and Increased steadily thereafter. The weights of liver, epididymal fat pads, and kidney increased in similar pattern as body weight, but their relative ratio to body weight decreased with age. The ratio of epididymal fat pads to body weight increased with age. The weight of extensor digitorium longus, coleus and plantaris increased from 1 to 4 months, but it decreased at 6, 12 months. The protein content of muscles decreased or increased through- out 12 months. The T cell proliferation response to Con A stimulation was significantly lower at 6 months than 1 month and lower at 12 months than 6 months.

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Effects of Dietary Protein and Lipid Levels on Growth and Body Composition of Juvenile Sea Cucumber Stichopus japonicus (배합사료의 단백질 및 지질 함량이 어린 돌기해삼 Stichopus japonicus의 성장 및 체조성에 미치는 영향)

  • Seo, Joo-Young;Choi, Jin;Kim, Guen-Up;Cho, Sung-Su;Park, Heum-Gi;Lee, Sang-Min
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.19-25
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    • 2008
  • We investigated the proper dietary protein and lipid levels for growth and body composition of juvenile sea cucumber Stichopus japonicus. Three replicate groups of the sea cucumber(average weight of 1.1 g) were fed the experimental diets containing different levels of protein(10, 20, 30 and 40%) and lipid(3, 7 and 11%) for 10 weeks. At the end of the feeding trial, survival of each group was over 87%, and there was no significant difference among the groups. Weight gain and specific growth rate(SGR) of sea cucumber were significantly affected by dietary protein(P<0.005) and lipid levels(P<0.001). The highest weight gain and SGR were observed when sea cucumber fed the diet containing 30% protein with 3% lipid(P<0.05). Weight gain and SGR of the sea cucumber fed the diet containing 3% lipid were higher than the other groups at the same protein level except for 40% protein group(P<0.05). Protein content of the sea cucumber fed the diet containing 30% protein with 3% lipid was significantly (P<0.05) higher than those fed 20% protein diets with 7% or 11% lipids and 10% protein diet with 7% lipid. Moisture, lipid and ash contents of the whole body were not significantly different among the groups. Fatty acid compositions such as linoleic acid, EPA(20:5n-3) and DHA(22:6n-3) of the whole body were affected by those of dietary lipid sources. The results of this study indicate that the diet containing 30% protein and 3% lipid is optimal for growth of juvenile sea cucumber.

Purification and Characterization of a Juvenile Hormong Binding Protein from Whole Body Homogenates of the Wax Moth, Galleris mellonella Final Instar Larvae (꿀벌부채명나방 종령유충에서 유약호르몬 결합단백질의 정제와 특성)

  • 안기흥;전상학;이경로
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.59-64
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    • 1998
  • A juvenile hormone binding protein (JHBP) has been isolated from the whole body homogenate of Galleria rnellonella final instar larvae by gel filtration. The isolated protein is homogenous as judged by column chromatography and gel electrophoresis in the presence and absence of denaturing agent. The JHBP has a relative molecular weight of 32 k by denaturing gel electrophresis and 28 k by gel filtration. The protein exhibits a dissociation constant of 3.9 x M for JH 111.

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