• BMB Reports
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• 제34권2호
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• pp.95-101
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• 2001

Tissue transglutaminase (TGII, $G{\alpha}h$) belongs to a family of enzymes which catalyze post-translational modification of proteins by forming isopeptides via $Ca^{2+}$-dependent reaction. Although TGII-mediated formation of isopeptides has been implicated to play a role in a variety of cellular processes, the physiological function of TGII remains unclear. In addition to this Tease activity, TGII is a guanosine triphosphatase (GTPase) which binds and hydrolyzes GTP It is now well recognized that the GTPase action of TGII regulates a receptor-mediated transmembrane signaling, functioning as a signal transducer of the receptor. This TGII function signifies that TGII is a new class of GTP-binding regulatory protein (G-protein) that differs from "Classical" heterotrimeric G-proteins. Regulation of enzyme is an important biological process for maintaining cell integrity. This review summarizes the recent development in regulation of TGII that may help for the better understanding of this unique enzyme. Since activation and inactivation of GTPase of TGII are similar to the heterotrimeric G-proteins, the regulation of heterotrimeric G-protein in the transmembrane signaling is also discussed.

• 대한본초학회지
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• 제30권1호
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• pp.59-65
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• 2015

Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell. Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of $TNF-{\alpha}$, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway. Results : The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$, $100{\mu}g$ and $200{\mu}g/mL$ had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract $50{\mu}g/mL$ significantly suppressed the increase in $TNF-{\alpha}$ protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$ and $50{\mu}g/mL$ significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract $25{\mu}g$, $50{\mu}g$ and $100{\mu}g/mL$ suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract $25{\mu}g$ and $100{\mu}g/mL$ significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell, Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis.

• Journal of Nutrition and Health
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• 제20권5호
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• pp.301-308
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• 1987

An attempt was made to assess nutrient intake of a group of rural housewives living in the area of Yeo-Ju, forty persons were surveyed between July 4 and 5, 1984. All foods and beverage were weighed and collected as consumed in the home over 24-hour periods and protein and ten inorganic element in the diets were assayed by laboratory analysis. 1) The average food intakes of the subject per day were 1105.7g, which consisted of vegetable food (93.1%), animal food 96.6%), and fats and oils (0.3% . The energy percentage of carbohydrate, protein, and fat were 79.5%, 13.4% and 7.1%, respectively, showing higher dependence on carbohydrate. 2) The protein intake calculated from food table was 64.98g. The contribution of animal proteins to total protein was only 18.6g, for below the recommenced allowance. 3) Analysed daily mean intake (% of RDA) of protein (g), Ca(mg), K(g), Na(g), Mg(mg), P(mg), Zn(mg), Cu (mg), Mn(mg), Fe(mg) and Cl(g) were 60.79(93.3%), 395(65.9%), 4.79(85.268%), 6.53(198-594%), 321.4(107.15%), 827.8(103.38%), 15.81(105.4%), 1.66(55-835), 5.12(102.205%), 14.12(78.44%), 9.57(188-563%), respectively.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9853-9857
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• 2014

Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.

• 한국어병학회지
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• 제27권2호
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• pp.99-105
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• 2014

Biosensors consist of biochemical recognition agents like antibodies immobilized on the surfaces of transducers that change the recognition into a measurable electronic signal. Here we report a piezoelectric immunosensor made to detect Vibrio vulnificus. A 9MHz AT-cut piezoelectric wafer attached with two gold electrodes of 5mm diameter was used as the transducer of the QCM biosensor with a reproducibility of ${\pm}0.1Hz$ in frequency response. We have tried different approaches to immobilize antibody on the sensor chip. Concerning the orientation of antibody for the best antigen binding capacity, the antibody was immobilized by specific binding to protein G or by cross-linking through hydrazine. In addition, protein G was cross-linked on glutaraldehyde activated immine layer (PEI) or EDC/NHS activated sulfide monolayer (MPA). PEI was found to be more effective to immobilize protein G following glutaraldehyde activation than MPA. However, hydrazine chip showed a better capability to immobilize more IgG than protein G chip and a higher sensitivity. The sensor system was able to detect V. vulnificus in dose dependent manner and was able to detect bacterial cells within 5 minutes by monitoring frequency shifts in real time. The detection limit can be improved by preincubation to enrich the bacterial cell number.

• 한국가정과교육학회지
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• 제24권2호
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• pp.51-62
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• 2012

본 연구는 1962년 한국인 영양권장량의 제정부터 2010년 한국인 영양섭취기준 개정에 이르기까지 성인과 임신 수유부의 단백질의 섭취권장량 산출방법과 섭취권장량의 변화 추이를 살펴보았다. 1. 성인의 단백질 섭취권장량은 1989년까지 요인가산법을, 그 이후에는 질소균형연구를 활용하여 설정되었다. 요인가산법을 적용하였던 시기는 표준단백질 최소 필요량 또는 불가피 질소손실량을 기본 요인으로 하고 식이단백질의 이용율을 적용하였으며, 질소균형연구를 적용하였던 시기는 질소평형 유지에 필요한 식이단백질의 최소 필요량을 기본 요인으로 하였다. 그리고 개인 차, 스트레스 등의 조정 요인을 반영하여 단백질 섭취권장량을 계산하였다. 단백질 섭취권장량은 남성 50~80g/일, 여성 45~70g/일이었으며, 남성의 섭취권장량이 여성보다 크고, 연령대가 높을수록 섭취권장량은 감소하였다. 2. 임신부의 단백질 부가섭취권장량은 태아의 발육에 기인하는 단백질 축적량을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 10~30g/일이었으며, 2010년에는 임신 기간을 3분하여 각각 0, 15, 30g/일을 제시하였다. 3. 수유부의 단백질 부가섭취권장량은 모유로 분비되는 단백질의 양을 기본 요인으로 하고 식이단백질 이용율 등의 조정요인을 적용하여 산출하였고, 20~30g/일이었으며, 시기별 증감 경향은 나타나지 않았다. 앞으로 체중 대신 제지방체중을 적용하는 단백질 섭취권장량의 산출 공식이 마련될 필요성이 있으며, 에너지와 마찬가지로 섭취하는 식이단백질의 조성에 근거한 개인별 섭취권장량의 계산방법 고안을 제언한다. 그리고 수유부의 경우 수유 기간을 구분하여 기간별 단백질 부가섭취권장량의 제정을 제언한다.

• 한국식품영양과학회지
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• 제23권5호
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• pp.711-717
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• 1994

This study was conducted to obtain accurate data on intake, apparent digestibility and nitrogen balance of dietary protein which the korean take in habitually. Subjects were 8 male college students, aged from 20 to 26, and maintained their menu and life pattern as usual during a 4-week study. The same amount of diet that the subjects had consumped, and feces and urine were collected and measured to extract their nitrogen content data by Kjeldahl method. From above data, the apparent digestibility and the body nitrogen balance were estimated by determining daily protein intake and excretion. The daily protein intake measured by Jjeldahil method was 88.3$\pm$ 0.9g(1.45/kg of body weight /day) which marked 8.3% higher than that estimatd by food analysis table. The proportional of animal protein against total protein intake was 50.4$\pm$ 2.3%. Daily fecal protein loss was 14.3$\pm$0.6g and the apparent digestibility was 83.8$\pm$ 0.7%. The urinary nitrogen excretion was 10.30$\pm$0.19g. The nitrogen balance considering nitrogen excretion from feces indicated positive balance of 1.06$\pm$0.20g.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.87-92
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• 2003

We describe here the cloning, expression and characterization of a cDNA encoding a putative chemosensory protein (CSP) from the mole cricket, Gryllotalpa orientalis. The G. orientalis chemosensory protein cDNA sequences comprised of 384 bp with 128 amino acid residues. The G. orientalis chemosensory protein showed 75.4% protein sequence identity to the Locusta migratoria CSP, Northern blot analysis revealed that signal was stronger in head than leg and cuticle, indicating that the head part containing antennae is a main site for G. orientalis chemosensory protein synthesis. The cDNA encoding G. orientalis chemosensory protein was expressed as approximately 12 kDa polypeptide in baculovirus-infected insect cells.

• Journal of Nutrition and Health
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• 제13권4호
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• pp.167-176
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• 1980

This study was conducted to compare effects of various types of dietary carboh ydrates fed with different levels of protein on the protein utilization in weanling rats. Sixty male Sprague-Dawley rats weighing $60{\pm}1.3grams$ were adapted for 1 week with 77% starch-15% casein diet. Then the animals divided into 12 groups according to body weight and fed each experimental diet for two weeks. Carbodydrates used were glucose, starch, and sucrose and the amount of protein given were 0g, 1g, 3g, 5g casein/day. Protein portion of the diet was fed in two seperate feedings per day while nonprotein portion was fed ad libitum. It seemed that there was no significant difference in the protein utilization by using the different kinds of carbohydrate, but in P.E.R., N.P.U., weights of organs and protein and lipid in total carcass, glucose groups were tended to be slightly lower than starch and sucrose groups. The larger the amount of casein given, the higher were the value of body weight gain, F.E.R., weights of organs, total lipid in carcass and the amount of nitrogen retention. On the while, the larger the amount of casein given, the lower were the value of the intake of non-protein portion, P.E.R., N.P.U, and the percentage of nitrogen retention.

• Journal of Photoscience
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• 제9권2호
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• pp.278-280
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• 2002

We have cloned a hydromedusan opsin cDNA and showed that the deduced amino acid sequence of the cytoplasmic loop between helices 5 and 6 (loop 5-6) was clearly different from that reported so far. The amino acid sequence of the loop 5-6 is important on determination of the specificity for the coupled G- protein. To clarify which class of G-protein mediates the phototransduction system in the ocellus of the hydromedusan, we investigated G-proteins expressed in the ocellus. By PCR against the cDNA of the ocellus with primers designed according to the conserved amino acid sequence in G-protein a subunit, we obtained three kinds of cDNA fragments. Based on the sequence similarities, ttwo of them (JGI and JG3) were classified as $G_{i}$ and $G_{q}$, respectively. The other one (JG2) was a new subtype within $G_{*}$ class. Electron microscopic immunocytochemistry with the antiserum against the C-terminal sequence of $G_{q}$ or $G_{t}$ revealed the presence. of the both classes in the ocellus. The similarity of the C-terminal sequence of the JG2 with that of bovine $G_{t}$ suggests that the anti- $G_{t}$ antiserum would bind to JG2. These results suggest the possibility that the hydromedusan rhodopsin decides the specificity for the coupled G-protein by the other domain than the loop 5-6.oop 5-6.5-6.