• 대한수의학회지
• /
• 제41권1호
• /
• pp.29-35
• /
• 2001

The protective effects of egg yolk atibodies obtained from chickens immunized with fimbrial antigens from ETEC 987P were evaluated in 14 and 21 d old pigs in which ETEC diarrhea was induced. For the Exp. 1, eight early-weaned pigs($5.00{\pm}0.5kg$ average BW and 14 d average age) and eight weaned pigs($6.00{\pm}0.5kg$ average BW and 21 d average age) were used to examine influence of egg yolk antibodies on growth performance and resistance to ETEC 987P infection. Dietary treatments included 1) administered of commercial egg yolk(14 d of age; CEY14), 2) administered of egg yolk antibodies(14 d of age; EYA14), 3) administered of commercial egg yolk(21 d of age; CEY21), 4) administered of egg yolk antibodies(21 d of age; EYA21). The 14 and 21 d old pigs were challenged with 2 ml of ETEC 987P at a dose of $10^{10}\;CFU\;ml^{-1}$ per weaned pigs. Weaned pigs treated with egg yolk antibodies recovered and pigs treated with egg yolk antibodies tended to increase average daily gain(P<0.05). Also, EYA12 and EYA21 treatments were reduced coli-form bacteria concentration and increased Lactobacilli sp. concentration from feces. For the Exp. 2, sixteen weaned pigs($6.00{\pm}0.5kg$ average daily gain BW and 21 d average age) were used to examine influence of yolk or white from egg containing antibodies on growth performance and resistance to ETEC 987P infection. Dietary treatments included l) administered of commercial egg yolk(CEY), 2) administered of commercial egg white(CEW), 3) administered of egg yolk antibodies(EYA), 4) administered of egg white antibodies(EWA). Pigs treated only with EYA showed signs of recovery. Also, EYA treatment showed the best average daily gain without significant differences (P>0.05). EYA treatment was reduced coli-form bacteria concentration increased and Lactobacilli sp. concentration from feces. In conclusion, egg yolk antibodies have protective effects from pigs in which ETEC diarrhea was induced.

• Biomolecules & Therapeutics
• /
• 제6권2호
• /
• pp.191-198
• /
• 1998

Vsrio vulnificus is an estuarine gram-negative human pathogen that affects people with chronic hepatitis, alcoholic cirrhosis, diabetes mellitus or other underlying diseases. V. vulnificus infection is mediated primarily by consumption of raw fish or by exposure of pre-existing wounds to seawater, causing permanent tissue damages or fatal septic shock. We have been developing a vaccine against V. vulnificus composed of whole cell Iysate of a V. vulnificus O-antigen serotype 4 strain. Oral administration of the V. vulnificus;oral vaccine;immunogenicity;protective efficacy vaccine elicited a high serum antibody response in rabbits. The induced antibodies were reactive not only to the homologous strain but also to heterologous O-antigen serotype strains, indicating cross-reactivities among serotypes. Western blot analysis revealed that the antibodies are mainly specific for outer membrane proteins (OMPs) and reacted equally well with OMPs purified from 9 O-antigen serotypes. The rabbit antisera showed opsonophagocytic killing activity against heterologous strains as well as the homologous strain. Passively transferred rabbit antisera into mice were protective against a lethal V. vulnificus infection. These data demonstrate that oral administration of the V. vulnificus vaccine induced a systemic antibody response which had a protective efficacy against V. vulnificus infections, suggesting that this vaccine preparation could be used to develop an oral vaccine against V. vulnificus.

• Parasites, Hosts and Diseases
• /
• 제55권3호
• /
• pp.255-267
• /
• 2017

Malaria is an infectious disease affecting humans, which is transmitted by the bite of Anopheles mosquitoes harboring sporozoites of parasitic protozoans belonging to the genus Plasmodium. Despite past achievements to control the protozoan disease, malaria still remains a significant health threat up to now. In this study, we cloned and characterized the full-unit Plasmodium yoelii genes encoding merozoite surface protein 1 (MSP1), circumsporozoite protein (CSP), and Duffy-binding protein (DBP), each of which can be applied for investigations to obtain potent protective vaccines in the rodent malaria model, due to their specific expression patterns during the parasite life cycle. Recombinant fragments corresponding to the middle and C-terminal regions of PyMSP1 and PyCSP, respectively, displayed strong reactivity against P. yoelii-infected mice sera. Specific native antigens invoking strong humoral immune response during the primary and secondary infections of P. yoelii were also abundantly detected in experimental ICR mice. The low or negligible parasitemia observed in the secondary infected mice was likely to result from the neutralizing action of the protective antibodies. Identification of these antigenic proteins might provide the necessary information and means to characterize additional vaccine candidate antigens, selected solely on their ability to produce the protective antibodies.

• Parasites, Hosts and Diseases
• /
• 제30권2호
• /
• pp.113-124
• /
• 1992

마우스에 N. fowleri를 감염시키기 전에 N( 2단세포군 항체를 2회 투여했을 때 사망률과 평균 생존기간이 15.8%, 17.7일, 1회 투여했을 때는 16.7%, 17.0일로 대조군의 22.7%, 14.6일 에 비해 사망률이 감소하고 생존기간이 연장되었다. Nf 154 단세포군 항체를 2회 투여시 사망률과 생존기간이 10.5%, 16.5일로 대조군에 비하여 차이가 있었다. 그러나 N. fowleri를 감염시킨 후 Nf 2 단세포군 항체를 2회 또는 1회 투여했을 때는 대조군과 비교하여 사망률과 생존기간의 차이 가 없음이 관찰되었다. 배양중인 N. fowleri 영양형에 Nf 2 및 Nf 154 단세포를 항체를 처리했을 때 대조군에 비해 현저한 응집반응이 관찰되었으며, 보체를 처리하여 영양형의 증식정도를 관찰한 결과 단세포를 항체 처리시 영양형의 증식이 현저히 감소하였다. Nf 2및 Nf 154단세포군 항체로 처리된 N. fowleri 영양형의 미세구조를 관찰하였는데, swelling된 미토콘드리아의 수가 증가하였으며 cisternae의 손상도 관찰되었다. 또한 lipid droplets가 나타나고 그수가 증가하였으며, peroxisome은 관찰되지 않았으며, 공포 수의 증가와 더불어 osmiophilic granules등이 관찰되었다. N. fowleri의 세포독성 실험에서 배양된 CHO세포에 N. fowleri만 넣었을 때, 세포독성이 73.0% 인데 비해, Nf 2 및 Nf 154 단세포를 항체를 넣었을 패는 각각 5.4%, 10.7%로 CHO세포에 대한 N. fowleri의 세포파괴율이 저하됨이 관찰되었다.

• 한국동물위생학회지
• /
• 제42권1호
• /
• pp.9-15
• /
• 2019

Transmissible gastroenteritis (TGE) is a disease confined to pigs of all ages, and can be a significant cause of economic loss in breeding herds, primarily because of the very high piglet mortality. The causative agent is a coronavirus, an enveloped positive strand RNA virus and closely related but non-enteropathogenic porcine respiratory coronavirus (PRCV). Although the TGEV has declined with its innocent relative, PRCV, further genome changes could not be excluded. Therefore, the herd-level immunity against this virus is important for the prevention of disease and should be carefully monitored. The aim of this study is to develop monoclonal antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) which can rapidly and accurately determine a large numbers of serum samples for surveillance purpose, and to compare the ELISA with a TGEV-specific serum neutralization test. The MAC-ELISA was sufficiently achieved, and the comparison with the virus-specific serum neutralization assays for 713 sera from pig farms showed a high correlation ($r^2=0.812$, P<0.001). The specificity and sensitivity of MAC-ELISA for the serum neutralization test 91.9% and 91.6%, respectively, which means that the antibody detected by the MAC-ELISA could be said to be protective antibodies. In conclusion, the developed MAC-ELISA would be very helpful in evaluating protective antibodies against TGEV.

• Journal of Microbiology
• /
• 제43권5호
• /
• pp.437-442
• /
• 2005

Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on $13\%$ SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.

• 미생물과산업
• /
• 제16권2호
• /
• pp.2-9
• /
• 1990

This paper outlines research to study two aspects of Cryptooridium. First, specific antigenic determinants were identified and followed through the growth cycle of C. parvum to investigate antigenic sharing of molecular epitopes among the different life cycle stages. Secondly, the importance of passive immune protective mechanisms in cryptosporidial infection was assessed by following the course of infection in neonatal mice which have been subjected to treatments using either monoclonal antibodies (mAbs) or hyperimmune bovine colostrum.

• 미생물학회지
• /
• 제43권1호
• /
• pp.7-13
• /
• 2007

Pasteurella multocida는 돼지에서 위축성 비염, 폐렴을 비롯한 다양한 호흡기 질환을 일으키는 병원균이다. 본 연구에서는 돼지 위축성 비염에 대한 효과적인 순수정제 백신을 개발하고자 하는 기초 연구로서 P. multocida의 외막 단백질 H에 의해 유도되는 방어적 항체와 면역을 확인하였다. P. multocida의 외막 단백질을 포함하는 분획은 호흡기 질병 혼합 백신에 대한 항혈청과 불활화된 사균 세포에 대한 항혈청 모두에서 면역학적으로 검출 가능하였다. 선행 연구에서 분리한 외막 단백질 H 유전자는 재조합 발현 백터 제작에 이용되어 대장균으로부터 재조합 외막단백질 H를 정제하였다. 실험 동물 면역과 항혈청의 교차반응, ELISA를 통한 항체 역가의 측정 및 공격접종을 통하여, 재조합 외막 단백질 H는 높은항원성을 가지며, 지속적인 체액성 면역을 유도하는 것을 확인하였다. 외막 단백질 H는 순수정제 항원으로서 P. multocida에 의한 호흡기 질환에 대한 효과적인 방어를 유도할 수 있는 단위 백신 후보 단백질로 여겨진다.

• 한국임상수의학회지
• /
• 제26권5호
• /
• pp.423-428
• /
• 2009

There were outbreaks of canine distemper in Korea from the late 1990's to the early 2000's even though modified live CDV vaccines had been used as the same way as before. The present study was undertaken to investigate the levels of neutralizing antibodies in the Korean dog population, and the factors associated with the levels, with special reference to the vaccination history of the dogs. A total of 772 serum samples were from clinically healthy dogs with over one year old throughout the Korea from January 2003 to April 2004. Details on the sex, breed, age, vaccination status and disease histories were recorded. The level of neutralizing antibodies titer was determined with a modified version of the microneutralization test. Titers over 16 were classified as protective CDV antibody titers. The overall rate of adult dogs with protective antibody titers was 96.0%. The dogs with protective antibody titers varied depending on age, sex, rearing environment and vaccination status. Because the majority of healthy adult dogs in Korea had adequate serum antibody titers against CDV and the immunity provided by the vaccinations is claimed to last for several years, annual revaccination protocol for CDV in adult dogs should be reconsidered.

• Journal of Microbiology and Biotechnology
• /
• 제25권2호
• /
• pp.268-273
• /
• 2015

The fluorescent-antibody-to-membrane-antigen (FAMA) test is regarded as the "gold standard" to detect protective antibodies to varicella-zoster virus (VZV) because of its high sensitivity and specificity. Because the classic FAMA test uses an infectious virus for detection of antibodies to VZV, it is labor-intensive, and also requires special equipment for handling the virus. For this reason, we attempted to develop a simple and safe FAMA assay. Because VZV glycoprotein E (gE) is one of the major VZV glycoproteins, we used the gE protein for the FAMA test (gE FAMA). Here, we demonstrate that overexpression of gE in HEK293T cells can be used to measure antibodies in human serum, and that gE FAMA titers are closely correlated with gpEIA ELISA data. These results indicate that our gE FAMA test has the potential to measure antibodies to VZV.