Browse > Article

Protective Antibodies and Immunity elicited by Immunization with Outer Membrane Protein H of Pasteurella multocida in Mice  

Kwon, Moo-Sik (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Young-Bong (Department of Animal Biotechnology, Konkuk University)
Lee, Jeong-Min (Department of Animal Biotechnology, Konkuk University)
Publication Information
Korean Journal of Microbiology / v.43, no.1, 2007 , pp. 7-13 More about this Journal
Abstract
Pasteurella multocida is one of the important animal pathogen causing widespread infections in various domestic animals. In swine, it causes severe respiratory diseases such as atrophic rhinitis and pneumonic pasteurellosis. To develop the efficient subunit vaccine against swine atrophic rhinitis, we investigated protective antibodies and humoral immunity of outer membrane protein H (OmpH) which is one of the major outer membrane proteins in P. multocida. Outer membrane fraction of P. multocida was immunologically detectable using antisera from both mice groups vaccinated by formalin-killed whole cells and by commercial vaccine. The expression vector for production of recombinant OmpH was constructed and the recombinant OmpH was expressed and purified from E. coli. Recombinant OmpH showed high antigenic and immunogenic properties in mice vaccination and ELISA with antisera.
Keywords
atrophic rhinitis; outer membrane protein H; Pateurella multocida; protective antibodies;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Poolman, J.T., L. Bakaletz, A. Cripps, P.A. Denoel, A. Forsgren, J. Kyd, and Y. Lobet. 2000. Developing a nontypeable Haemophilus influenzae (NTHi) vaccine. Vaccine 19 (suppl 1), S108   DOI   ScienceOn
2 To, H., S. Someno, and S. Nagai. 2005. Development of a genetically modified nontoxigcnic Pasteurella multocida toxin as a candidate for use in vaccines against progressive atrophic rhinitis in pigs. Am. J. Vet. Res. 66, 113-120   DOI   ScienceOn
3 Carter, G.R. 1967. Pasteurellosis: Pasteurella multocida and Pasteurella heinolytica. Adv. Vet. Sci. Camp. Med. 11, 321-379
4 Christodoulides, M., B.T. McGuinness, and J.E. Heckels. 1993. Immunization with synthetic peptides containing epitopes of the class 1 outer-membrane protein of Neisseria meningitidis: production of bactericidal antibodies on immunization with a cyclic peptide. J. Gen. Microbiol. 139, 1729-1738   DOI   PUBMED   ScienceOn
5 Harper, M., J. D. Boyce, and B. Adler. 2006. Pasteurella multacida pathogenesis: 125 years after Pasteur. FEMS Microbiol. Lett. 265, 1-10   DOI   ScienceOn
6 Liao, C.M., C. Huangb, S.L. Hsuan, Z.W. Chenb, W.C. Lee, C.I. Liu, J.R Winton, and M.S. Chien. 2006.Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs. Vaccine 24, 27-35   DOI   ScienceOn
7 Luo, Y., J.R. Glisson, M.W. Jackwood, R.E. Hancock, M. Bains, I.H. Cheng, and C. Wang. 1997. Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73. J. Bacteriol. 179, 7856-7864   DOI   PUBMED
8 김봉환, 탁연빈, 조길재, 장희경. 1991. 돼지 전염성 위축성 비염의 임상학적 및 세균학적 연구. 대한수의학회지 31, 457-469
9 Adler, B., D. Bulach, J. Chung, S. Doughty, M. Hunt, K Rajakumar, M. Serrano, A van Zanden, Y. Zhang, and C. Ruffolo. 1991. Candidate vaccine antigens and genes in Pasteurella multocida. J. Biotechnol. 73, 83-90   DOI   ScienceOn
10 Heddleston, K.H., J.E. Gallagher, and P.A. Rebers. 1972. Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis. 16, 925-936   DOI   ScienceOn
11 Petersen, S.K., N.T. Foged, A. Bording, J.P. Nielsen, H.K. Riemann, and P.L. Frandsen. 1991. Recombinant derivatives of Pasteurella multocida toxin: candidates for a vaccine against progressive atrophic rhinitis. Infect. Immun. 59, 1387-1393   PUBMED
12 Vasfi, M. and K.R. Mittal. 1997. Role of outer membrane protein H (OmpH)- and OmpA-specific monoclonal antibodies from hybridoma tumors in protection of mice against Pasteurella multocida. Infect. Immun. 65, 4502-4508   PUBMED
13 Hunt, M.L., B. Adler, and K.M. Townsend. 2000. The molecular biology of Pasteurella multocida. Vet. Microbiol. 72, 3-25   DOI   ScienceOn
14 Lee, J., S. Kang, S.I. Park, H.J. Woo, and M. Kwon. 2004. Molecular cloning and characterization of the gene for outer memebrane protein H in a Pasteurella multocida (D:4) isolate from pigs with atrophic rhinitis symptoms in Korea. J. Microbiol. Biotechnol. 14, 1343-1349
15 Rhoades, K.R. and R. B. RimIer. 1987. Capsular groups of Pasteurella multocidaisolated from avian hosts. Avian Dis. 31, 895-898   DOI   ScienceOn
16 지영철, 로승, 한정희, 한태욱. 2000. 돼지 위축성 비염백신의 효과에 관한 연구. 대한수의학회지 40, 707-717
17 Luo, Y., Q. Zeng, J.R. Glisson, M.W. Jackwood, I.H. Cheng, and C. Wang. 1999. Sequence analysis of Pasteurella multocidamajor outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge. Vaccine 17, 821-831   DOI   ScienceOn
18 Rajeev, S., R.V. Nair, S.A. Kania, and D.A. Bemis. 2003. Expression of a truncated Pasteurella multocida toxin antigen in Bordetella bronchiseptica. Vet. Microbiol. 94, 313-323   DOI   ScienceOn
19 Rutter, J.M. and X. Rojas. 1982. Atrohpic rhinitis in gnobiotic piglets: differences in the pathogenicity of Pasteurella multocida in combined infections with Bordetella bronchiseptica. Vet. Res. 110, 531-535
20 Gatto, N.T., S.M. Dabo, R.E. Hancock, and A.W. Confer. 2002. Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multacida serotype A:3 (Omp28). Vet. Microbiol. 87, 221-235   DOI   ScienceOn
21 Simons, K.R., R.J. Morton, R.W. Fulton, and A.W. Confer. 1992. Comparison of antibody responses in cattle to outer membrane proteins from Pasteurella haemolytica serotype I and from eight untypeable strains. Am. J. Ves. Res. 53, 971-975