• Title/Summary/Keyword: protected culture

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Antioxidant and Anti-inflammatory Effects of Plant Extracts from Bangladesh (방글라데시 식물 추출물의 항산화 및 항염 효과 연구)

  • You, SoHyeon;Kim, Gun-Hee
    • Journal of the Korean Society of Food Culture
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    • v.35 no.6
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    • pp.605-612
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    • 2020
  • In this study, 11 plant extracts from Bangladesh were used to evaluate the total phenolic and flavonoid content, in vitro antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzthiazolin-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay. Also, the inhibitory effect of nitric oxide (NO) production in RAW 264.7 macrophage cell line and the neuroprotective effect on H2O2-induced PC12 cells were tested. Our results revealed that Piper betle L. showed the highest total phenolic content (162.2 mg GAE/g extract) among the 11 plants from Bangladesh. Most plants showed strong radical scavenging effects and ferric reducing antioxidant power. Besides, Piper betle L. protected PC12 neuronal cells against H2O2 related oxidative stress in LPS-induced PC12 cells. Regarding the anti-inflammatory effect, Piper betle L. significantly inhibited NO accumulation in LPS-induced RAW 264.7 cells. Our results provide evidence that Piper betle L. could be useful for the development of functional health foods.

Effects of Dietary Dae-Chu (Rhamnace ziziphus), Onion (Allium cepa L.), Mixture Extract on Serum Composition in Rats (대추, 양파, 혼합추출물이 흰쥐의 혈청조성에 미치는 영향)

  • Cho, Young-Ja;Park, Hee-Jin;Ju, Sung-Mee;Hou, Won-Nyoung
    • Journal of the Korean Society of Food Culture
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    • v.21 no.1
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    • pp.99-106
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    • 2006
  • The purpose of this study was to investigate the effect of dietary dae-chu(Rhamnace ziziphus, A), onion(Allium cepa L., O), mixture extracts (mulberry leaf, licorice root, pine needle, angelica gigas, jujube, onion, M) on serum glucose, lipid, enzyme, phosphorus levels in rats (Sprague-Dawley male rats, $357.03{\pm}7.08g$). Serum calcium of onion group was significantly decreased (p<0.05), but mixture extracts group of Cl (p<0.05) and TBIL (total bilirubin, p<0.05) were significantly increased. Serum glucose, total cholesterol, HDL-cholesterol and triglyceride were increased experimental rats than those of the normal rats. Mixture extracts was better than other groups for lipid metabolism. Also, GPT(glutamic pyruvic transaminase) and GOT(glutamic oxaloacetate transaminase) of onion extracts were protected to liver. So mixture and onion extracts were good drink for health.

Isolation and Identification of High Phosphate-accumulating Bacteria (인 축정능이 우수한 세균의 분리 및 동정)

  • 신공식;고정연;최우영
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.286-291
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    • 1999
  • By using autoradiography and phosphate medium, high phosphate accumulating bacteria were isolated from the soil of protected cultivation area and activated sludge. Selected strain, PO8, was gram-negative, rod/spherial(0.5~0.6$\times$1.0~1.2${\mu}{\textrm}{m}$ in size) and non-motile. PI4, another selected strain, was gram-negative, rod(0.4~0.5$\times$1.5~1.6${\mu}{\textrm}{m}$ in size) and motile. It also had flagella. According to their morphological, physiological and biochemical properties, the stains were identified as Acinetobacter lwoffi PO8 and Chromobacterium lividum PI4, respectively. A. lowoffi PO8 and C. lividum PI4 cultured in the P-1 medium containing 150ppm phosphate were able to uptake high phosphate up to 92% and 85%, respectively after 24 hours at 3$0^{\circ}C$ during liquid culture.

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Identification of to Hexapeptides that Render C2 Myoblasts the Resistant Menadione-induced Cell Death

  • Hwang, Sung-Ho;Kim, Min-Jeong;Lim, Jeong-A;Woo, Joo-Hong;Kim, Hye-Sun
    • Animal cells and systems
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    • v.12 no.1
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    • pp.35-39
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    • 2008
  • Menadione induced cell death in cultured C2 myoblasts. By screening synthetic peptide libraries composed of random sequence of hexapeptides, we identified the hexa-peptides pool of(Ala/Ile)-(Ile/Met)-Val-Ile-Asp-(Met/Ser)-$NH_2$ that protected the myoblasts against menadioneinduced cell death. Pre-incubation with the hexapeptide pool reduced the number of cells detached from culture dish substrate and increased the ratio of relative viability against menadione. In addition, the peptides strongly increased the expression of Bcl-2, an anti-apoptotic protein. These results suggest that the hexapeptides might enhance the resistance to cell death against menadione by increasing the expression of Bcl-2.

The Vegetation of the Catba national Park in Vietnam (베트남 캣바국립공원의 식생)

  • ;Thin, N. N.
    • The Korean Journal of Ecology
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    • v.21 no.2
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    • pp.139-149
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    • 1998
  • The Catba national park (20$\circ$42'-20$\circ$54'N, 106$\circ$54'-107$\circ$09'E), which is a representative protected area in northeast Vietnam, was first investigated in terms of phytosociology of the Zurich-Montpellier School. 15 plant communities were identified from the seven vegetation types: Teetaria-Amoora gigantea community, Blechmum-Pitecellobium ferrugineum community, Impatiens-Dracaena cambodiana community, Rhizophora apiculata community, Avicenia marina community, Panicum repens community, Heterosmilax-Zanthoxylum nitidum community, Stachytarphyta jamaiensis-Bidens bipinnata community, microstegium vagans community, Dicranopteris linearis community, Randina-Sapium sebiferum community, Psidium gujava community, Elephantopus scaber community, and chirita aratformis community. Traditional pasturing and shifting agrie\culture by indigenous people in Catba national park were recognized as main disturbance regies, which have been far above sustainable levels. Ecological strategies for conservation and sustainable use on national park' ecosystem were proposed: (1) development of awareness program on sustainable life style of indigenous people, (2) establishment of multiple use module system of national park, (3) ecosystem monitoring of permanent ecological sites.

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Enactment of Physical Therapy Abbreviations and Symbols in Korea (물리치료 약어와 기호의 제정)

  • Bae Sung-Soo
    • The Journal of Korean Physical Therapy
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    • v.14 no.3
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    • pp.116-128
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    • 2002
  • Physical therapy record what the therapist dose to manage the individual patients case. The rights of the therapist and patients are protected should any question occur in the future regarding the care provided to the patient. Physical therapy note is a method of communicating with the patient's physician, other health care professionals and including other therapists. Abbreviations and symbols are used as a time and space saver while writing physical therapy Progress note. In order to ensure that everyone in the hospital need understand what has been written in the chart by others. Therefore, we need a enactment of physical therapy abbreviations and symbols in Korea. There are 468 abbreviations and symbols. It will be change with development of medical science, physical therapy and culture.

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Enactment of Physical Therapy Abbreviations and Symbols in Korea (물리치료 약어와 기호의 제정)

  • Bae Sung-Soo
    • The Journal of Korean Physical Therapy
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    • v.14 no.4
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    • pp.234-259
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    • 2002
  • Physical therapy record what the therapist dose to manage the individual patients case. The rights of the therapist and patients are protected should any question occur in the future regarding the care provided to the patient. Physical therapy note is a method of communicating with the patient's physician, other health care professionals and including other therapists. Abbreviations and symbols are used as a time and space saver while writing physical therapy progress note. In order to ensure that everyone in the hospital need understand what has been written in the chart by others. Therefore, we need a enactment of physical therapy abbreviations and symbols in Korea. There are 468 abbreviations and symbols. It will be change with development of medical science, physical therapy and culture.

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A Study on the investigation about the renovation project of the Modern Age Architecture (근대건축물의 리노베이션 방법에 관한 연구)

  • Seo, Sung-Ho;Park, Chan-Il
    • Proceedings of the Korean Institute of Interior Design Conference
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    • 2005.10a
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    • pp.71-75
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    • 2005
  • For last century, our country fast changed by development of industry but we justly looked away that cultural and natural environment that must preserve inside the change, and we sought quantitative growth. But consciousness about culture is rising now; thereby social request becomes strong that is improved cultural environment; and viewpoint is appearing which is thought heftily cultural environment of city. Important thing of them is the modern age architecture, but serious discord is happening in the current society that allows conservation and destruction of the modern age architecture. But, visible cultural asset such as the modern age architecture is value when it protected as the first time, When it was broken! , restoration or age architecture really necessary to us.

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Effects of Food Components on the Antibacterial Activity of Chitosan against Escherichia coli

  • Hong, Yi Fan;Moon, Eun-Pyo;Park, Yun-Hee
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1365-1367
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    • 2008
  • The antibacterial activity of chitosan against Escherichia coli was investigated in the presence of NaCl, sucrose, and ethanol to assess the potential use of chitosan as a biopreservative in food products containing these components. The inhibitory activity of chitosan decreased slightly upon the addition of NaCl and sucrose, respectively to culture broth containing 100 ppm of chitosan (Mw 3,000), while the addition of ethanol enhanced the inhibitory activity of chitosan on growing cells. The addition of these components to non-growing cells prior to chitosan treatment demonstrated that NaCl protected the cells from the inhibitory activity of chitosan, while sucrose had no effect. Ethanol addition to non-growing cells increased cell death by chitosan treatment. Finally, binding of fluorescein isothiocyanate (FITC)-labeled chitosan to E. coli was measured in the presence of the food components. The FITC-labeled chitosan binding to cells decreased upon NaCl addition, was not affected by sucrose, and increased following treatment with ethanol.

Factors Influencing Protoplast Isolation and Culture in Three Capsicum Species (세 가지 고추속 식물의 원형질체 분리 및 배양에 미치는 요인)

  • 임학태;염옥희;전익조;조미애;양승균
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.3
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    • pp.141-146
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    • 2001
  • Protoplasts were isolated from cotyledons, hypocotyls, and mesophyll tissues of three species of Capsicum species (C. anuumm, C. bacatuum, and C. chacoense). Combination of Cellulysin (1%) and Macero-zyme (0.25%) in 0.65 M sorbitol was found to be the most effective for the digestion of cell wall, regardless of the Capsicum species. Antioxidant MES (2-[N-Morpholino]ethanesulfonic acid) in the enzyme solution helped protoplasts overcome browning. After 5 days of initial culture, Cell division occurred in modified K8p medium containing 1~5 mg/L zeatin, 0.5 mg/L IAA, 0.1~0.5 mg/L TDZ, and 1 mg/L 2,4-D under continuous dark condition at $25^{\circ}C$. Semi-solid agarose culture method was more effective than liquid culture, and it also protected the cells from browning caused by polyphenolic compound released during protoplast culture. A total of 4000 calli were obtained from protoplast culture of different capsicum species. All of these calli were transferred to the 100 combinations of regeneration media using various plant growth regulators; TDZ, IAA, 2ip, BAP, NAA, and zeatin. These calli derived from protoplast of three species of capsicum were, however, not differentiated into shoots.

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