• Title/Summary/Keyword: protected culture

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An Examination of Food Intake and Nutritional status of the Koreans by Walks of Life during the Period of Japanese Ruling (일제하 한국인의 식품 섭취 및 생활 계층별로 본 영양소 섭취량에 관한 연구)

  • Kim, Sung-Mee;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.4 no.1
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    • pp.71-82
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    • 1989
  • While ruling Chosun, with a view to making Chosun the primary supplier of food, Japan made the peasantry of Chosun go to ruin by leaving land from them through land enterprises, and the projects of increasing rice production. At the same time, Japan formed the higher classes comprising pro-Japanese capitalists, landlords and intellectuals, and protected them in order to carry out her colonial policies. Naturally there came into being a great gulf between the minority of high society and the majority of the poor in Chosun. As there was a great difference in food life between the two, I'm going to examine the literature of those days to grasp exactly the condition of their food intake. As for the staple food, out of thirteen provinces in Chosun, 13% lived on only rice and 27% on other minor cereals with no rice. As for the subsidiary food, about thirty percents did not take any animal protein. The examination of intake of nutrition by classes shows that the higher and middle classes took the necessary amount of calorie and protein and that the component ratio of calorie was comparatively properly distributed. The lower classes are defined as those whose monthly income was less than 100 won and the peasantry in general. And again the peasantry are classified into three-high, middle and low-according to their farming conditions. The tenant farmers in Kyeongguido and the peasants of Darli community took enough amount of calorie and protein, but much smaller amount of animal protein. Fire-field farmers led not less miserable food intake than the extremely poor peasants. They seldom lived on rice. Potatoes, oats and millets were their staple food. Lastly, Engel's coefficient for the Tomack-min (the residents in mud huts) who were among the three extremely poor classes, was 73.3%, which was much higher than that of the lower classes in then Japan. Rationed rice and barley were their staple food but the rationed amount was not sufficient to satisfy needs of physical labor. In conclusion, during the period of Japanese ruling of Chosun, the minority of higher and middle classes in Chosun generally took sufficient amount of nutrient, while the status of food intake with poor peasants, fire-field farmers and Tomack-min was extremely miserable.

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Study for Diagnostic Efficacy of Minibronchoalveolar Lavage in the Detection of Etiologic Agents of Ventilator-associated Pneumonia in Patients Receiving Antibiotics (항생제를 사용하고 있었던 인공호흡기 연관 폐렴환자에서의 원인균 발견을 위한 소량 기관지폐포세척술의 진단적 효용성에 관한 연구)

  • Moon, Doo-Seop;Lim, Chae-Man;Pai, Chik-Hyun;Kim, Mi-Na;Chin, Jae-Yong;Shim, Tae-Sun;Lee, Sang-Do;Kim, Woo-Sung;Kim, Dong-Soon;Kim, Won-Dong;Koh, Youn-Suck
    • Tuberculosis and Respiratory Diseases
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    • v.47 no.3
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    • pp.321-330
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    • 1999
  • Background : Early diagnosis and proper antibiotic treatment are very important in the management of ventilator-associated pneumonia (VAP) because of its high mortality. Bronchoscopy with a protected specimen brush (PSB) has been considered the standard method to isolate the causative organisms of VAP. However, this method burdens consumer economically to purchase a PSB. Another useful method for the diagnosis of VAP is quantitative cultures of aspirated specimens through bronchoscopic bronchoalveolar lavage (BAL), for which the infusion of more than 120 m1 of saline has been recommended for adequate sampling of a pulmonary segment. However, occasionally it leads to deterioration of the patient's condition. We studied the diagnostic efficacy of minibronchoalveolar lavage (miniBAL), which retrieves only 25 ml of BAL fluid, in the isolation of causative organisms of VAP. Methods: We included 38 consecutive patients (41 cases) suspected of having VAP on the basis of clinical evidence, who had received antibiotics before the bronchoscopy. The two diagnostic techniques of PSB and miniBAL, which were performed one after another at the same pulmonary segment, 'were compared prospectively. The cut-off values for quantitative cultures to define causative bacteria of VAP were more than $10^3$ colony-forming units (cfu)/ml for PSB and more than $10^4$ cfu/ml for BAL. Results: The amount of instilled normal saline required to retrieve 25 ml of BAL fluid was $93{\pm}32 ml$ (mean${\pm}$SD). The detection rate of causative agents was 46.3% (19/41) with PSB and 43.9% (18/41) with miniBAL. The concordance rate of PSB and miniBAL in the bacterial culture was 85.4% (35/41). Although arterial blood oxygen saturation dropped significantly (p<0.05) during ($92{\pm}10%$) and 10 min after ($95{\pm}3%$) miniBAL compared with the baseline ($97{\pm}3%$), all except 3 cases were within normal ranges. The significantly elevated heart rate during ($l25{\pm}24$/min, p<0.05) miniBAL compared with the baseline ($1l1{\pm}22$/min) recovered again in 10 min after ($111{\pm}26$/min) miniBAL. Transient hypotension was developed during the procedure in two cases. The procedure was stopped in one case due to atrial flutter. Conclusion: MiniBAL is a safe and effective technique to detect the causative organisms of VAP.

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Effect of Irrigation Methods for Reducing Drainage on Growth and Yield of Paprika (Capsicum annuum 'Coletti') in Rockwool and Cocopeat Culture (배액절감형 양액공급 방법이 파프리카(Capsicum annuum 'Coletti') 생육과 수량에 미치는 영향)

  • An, Chul Geon;Hwang, Yeon Hyeon;An, Jae Uk;Yoon, Hae Suk;Chang, Young Ho;Shon, Gil Man;Hwang, Seung Jae;Kim, Kang Soo;Rhee, Han Cheol
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.228-235
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    • 2012
  • This study was carried out to investigate the effect of irrigation methods for reducing a drainage on the growth and yield in rockwool (Grodan co.) and cocopeat (chip : dust = 50 : 50 included fiber) culture. The nutrient solution was irrigated by $100J{\cdot}cm^{-2}$-100 mL, $50J{\cdot}cm^{-2}$-45 mL, $50J{\cdot}cm^{-2}$-40 mL, $50J{\cdot}cm^{-2}$-35 mL ($100{\sim}50J{\cdot}cm^{-2}$-100~35 mL, Nutrient solution 100~35 mL was irrigated per plant when the accumulated radiation was $100{\sim}50J{\cdot}cm^{-2}$). The drain rates per plant of 100-100, 50-45, 50-40, 50-35 were 26.3%, 8.8%, 6% 4.4% and 23.1%, 7.5%, 5% 3.4% in rockwool and cocopeat slabs. The water contents and EC of 100-100 and 50-45 were managed by the 55~70%, $3.0{\sim}5.0dS{\cdot}m^{-1}$ which were good condition for paprika culture in rockwool and cocopeat slabs, while those of 50-40 and 50-35 were managed by beyond 50%, $4.5{\sim}9.5dS{\cdot}m^{-1}$. The plant height, number of branches and leaf size of 100-100 and 50-45 were similarly increased while those of 50-40 and 50-35 were decreased. The fruit size and weight of 50-40 and 50-35 were small and light, while those of 100-100 and 50-45 were similarly big and heavy. The marketable fruits of 100-100 and 50-45 treatments were similarly more by 9.7~9.8 in rockwool and 8.8~8.9 in cocopeat, while the unmarketable fruits, the small and blossom end rot fruits were increased in 50-40 and 50-35 treatments. The yield of 100-100 and 50-45 treatments were similarly high.

Control of Irrigation Amount for Production of High Quality Fruit in Melon Fertigation Cultivation (멜론 관비재배시 고품질 과실생산을 위한 관수량 조절)

  • Rhee, Han-Cheol;Cho, Myeung-Whan;Eom, Young-Cheol;Park, Jin-Meun;Lee, Jae-Han
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.288-292
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    • 2008
  • This study was conducted to identify the effects of irrigation amount to produce high quality melon fruit in fertigation culture. Irrigation amount of during fruit harvesting period was doubled at the low irrigation point ($(-45{\sim}50\;kPa$) treatment as 115 mm as than that of the high irrigation point ($-20{\sim}25\;kPa$) treatment. The plant growth rates such as stem length, leaf weight and plant height were a little diminished at the low irrigation point ($-45{\sim}50\;kPa$) than those of the other treatments. Internode length was however not affected by irrigation amount. Fruit weight was lighter at the low irrigation point ($-45{\sim}50\;kPa$) than that of at the high irrigation point and fruit height was shorter, but fruit diameter was not affected by irrigation amount. Fruit soluble solid was $0.9^{\circ}Bx$ higher at the low irrigation point ($-45{\sim}50\;kPa$) than at the high irrigation point ($-20{\sim}25\;kPa$) and net index was higher. Total marketable yield was highest by 3,937 kg/10a at the high irrigation point ($-20{\sim}25\;kPa$), but the excellent marketable yield was highest by 2,531 kg/10a at the low irrigation point ($-45{\sim}50\;kPa$). Inorganic contents of the soil N, K, Ca and Mg were not affected by irrigation amount. It was therefore thought that optimum irrigation point to produce high quality melon fruit by fertigation culture was $-45{\sim}50\;kPa$ at ripening stage.

Isolation and Evaluation of an Antiviral Producing Serratia spp. Strain Gsm01 against Cucumber mosaic virus in Korea (한국에서 CMV에 항바이러스 효과를 나타내는 Serratia spp. Gsm01 균주의 분리 동정 및 효과 검정)

  • Ipper, Nagesh S.;Lee, Seon-Hwa;Suk, Jung-Ki;Shrestha, Anupama;Seo, Dong-Uk;Park, Duck-Hwan;Cho, Jun-Mo;Park, Dong-Sik;Hur, Jang-Hyun;Lim, Chun-Keun
    • The Korean Journal of Pesticide Science
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    • v.10 no.4
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    • pp.344-350
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    • 2006
  • An Antiviral producing bacterial strain was isolated from ginseng root environment in Hongcheon, Kangwon province of Republic of Korea. Identification of this bacterial strain was performed by physiological and biochemical tests along with 16S rRNA analyses. The results revealed that the bacterium was closer to genus Serratia, which was named as Gsm01. The strain was grown in Mannitol-Glutamate-Yeast (MGY) broth for 48 h. The culture was centrifuged and the filtrate obtained was tested for its ability to control Cucumber mosaic virus strain Y (CMV-Y) in greenhouse and field experiments. In the green house experiments, CF was evaluated for its ability to protect local host, Chenopodium amaranticolor and systemic host of CMV, Nicotiana tabacum cv. Xanthi-nc. It was found that, CF treatment reduced viral infection by 98% in local host; C. amaranticolor. The N. tabacum cv. Xanthi-nc plants treated with CF did not show visible viral symptoms 15 days post inoculation (dpi) and remained symptomless throughout the periods of the study. To evaluate effectiveness of CF under field conditions, experiment was carried out in a polyvinyl house. It was observed that, 52% plants were protected from viral diseases compared to non-treated plants, increasing the crop yield. This is the first report showing antiviral activity of a Serratia spp. against CMV.

A Study on the Application of Other Effective Area-based Conservation Measures(OECMs) for Natural Heritage - Focusing on the Old Big Trees of Natural Monument and Dangsan Ritual - (자연유산의 '기타 효과적인 지역기반 보전수단(OECMs)' 등재기준 적용 연구 - 천연기념물 노거수와 당산제를 중심으로 -)

  • Jun, Da-Seul;Shin, Hyun-Sil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.40 no.3
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    • pp.1-9
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    • 2022
  • This study compared and reviewed the recognition determinants by applying the OECMs criteria, focusing on old big trees, plant of natural monument that are natural heritage under the national heritage system of the Cultural Heritage Administration, and the results are as follows. First, among the protected areas designated and managed by government agencies according to each protection purpose, it is necessary to actively introduce new conservation measures, OECMs, to fulfill the Biodiversity strategy for 2030 while the land area is already saturated. Second, the OECMs are geographically defined areas(CBD, 2018), not currently recognized as a protected areas, governed and managed in a way that achieves positived sustained and effective contribution to in situ conservation of biodiversity. Since the selection of term, the scope of application criteria, and the context of interpretation are inevitably different, it is necessary to separately legislate and establish related laws of the OECMs suitable for each country's situation. Third, as a result of reviewing the OECMs criteria for plant of natural monument, the final 58 potential resources were recognized. Important elements among the OECMs criteria are that buffer zones should be spaced apart from designated zones to secure a certain area, and that economic activities through commercial production should not occur and meet biodiversity standards. Among the potential candidates, 23 areas were analyzed to be geographically isolated and independent, such as Forest of Oriental Arborvitae in Do-dong, Daegu, and forest types such as Carstor Aralia of Gungchon-ri, Samcheok and Forest of Common Camellias in Maryang-ri, Seocheon. As a result of reviewing the application of OECMs criteria for plant of natural monument, it was confirmed that the functions as a traditional uses were specialized among the values of biodiversity, and ecosystem services and cultural and spiritual values were inherited through Korea's unique culture of old big trees and Dangsan ritual. In terms of biodiversity criteria, it can be used as an important factor in connecting human and natural ecosystem networks without the discovery of new species.

Effects of Planting Date on the Growth and Yield of Okra (Abelmoschus esculentus) in Rain Shielding Vinyl House of Busan Area (부산지역에서의 오크라 비가림재배시 정식시기가 생육 및 수량에 미치는 영향)

  • Kim, Young Suk;Yoo, Mi Bok;Nam, Chun Woo;Kim, Tae Soo;Kim, Jae Suk;Seong, Ki Cheol;Rhee, Han Cheol
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.298-302
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    • 2013
  • The experiment was carried out to examine the effect of planting date on the growth and marketable yield on rain shielding vinyl house in Busan area. Okra cultivars were 'Jukokra', 'Greensod', 'Marumichang', 'Betafive' and 'Akamarumichang'. Planting date were June and July. Plant height, stem length and leaf width were significantly different between various cultivars. Plant height and stem length were best at 'Akamarumichang' among all cultivars tested and the 'Betafive' cultivars showed the lowest plant growth of June treatment. There was no significant difference in planting of July treatment. Marketable yield of okra fruit was affected by planting date. In case of June planting date, 'Betafive' cultivars produced the highest marketable yield (4,286 kg/10a) in green fruit. Marketable yield of green okra fruit was increased at 'Betafive' and 'Greensod' cultivars, whereas 'Marumichang' cultivars was lowerd. Therefore, the optimum planting date was considered June planting in rain shielding culture of okra in Busan area.

Suppression of Citrus Melanose on the Leaves Treated with Rhizobacterial Strains after Inoculation with Diaporthe citri (식물근권세균 처리에 의한 감귤 검은점무늬병에 대한 방제 효과)

  • Ko, Yun Jung;Kang, So Young;Jeun, Yong Chull
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.331-337
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    • 2012
  • Citrus melanose is one of important disease in citrus cultivation, reducing quality of citrus fruits and resulting in economic loss. Like other diseases in citrus, melanose was mainly protected by chemical fungicide in the field. Recently, alternative method of disease control is highly required due to the side effect of the chemicals. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3, and TRH415-2 are selected by dual-culture testing its antifungal activity against Diaporthe citri causing citrus melanose. To investigate the protection efficacy of the selected rhizobacterial strains to citrus melanose, the bacteria were pre-treated on citrus leaves following inoculation with melanose pathogen. Pre-treatment with all selected rhizobacterial strains showed disease suppression in which the levels of protection rates were different by the rhizobacterial strains. Additional treatment with the rhizobacterial strains after the pathogen inoculation enhanced protection rates in all cases. The strain MRL408-3 and TRH423-3 were identified as Burkholderia gladioli, TRH415-2 as Pseudomons fluorescens and THJ609-3 as Pseudomonas pudia as a result of analyzing the internal transcript spaces of the rhizobacterial strains rDNA. The selected rhizobacterial strains may be valuable as biological control agents in the environment-friendly citrus farm in which chemical application is limited.

Determination of Antigenicity and Characterization of Proteinase from Tissue Invading Nematode Larvae (조직기생 선충류 유충에서 분리한 단백 분해 효소의 특성 및 항원성 검토)

  • Rim, Han-Jong;Joo, Kyeong-Hwan;Choi, Sung-A;Lee, Hye-Jeong;Joo, Chong-Yoon;Chung, Myung-Sook
    • Journal of agricultural medicine and community health
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    • v.22 no.1
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    • pp.61-74
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    • 1997
  • In case of tissue invading nematode, proteolytic enzyme was required at their parasitic life. Proteinases obtained from these parasites(Toxocara canis, Ansakis spp. and Trichinella spiralis) were extracted, isolated and further purified. And then the analysis for activity and inhibitory effect of proteinases were performed by appropriate substrate. Determination of protein as a circulating antigen was done in use of infected animal serum with above parasites, respectively. For above experimental objects, following procedures were performed. First, enzymatic activity was measured in use of azocasein and inhibitory effect of porteinase were studied by various inhibitors. Second, partially purified proteins containing enzymatic activity were obtained by ion exchange chromatography, ultrafiltration and electrophoretic elution. Third, role of the partially purified protein as a circulating antigen. The results obtained were as follows : 1. Enzymatic activity of each nematode proteinase was varied according to pH. Optimal pH of Toxocara canis, Ansakis spp. and Trichinella spiralis were pH 6.0, pH 5.5 and pH 6.5, respectively. The optimal molarity of buffer was 0.1M phosphate buffer. Although little difference between these proteinases was observed, temperature stability was at least maintained at $4^{\circ}C$ until 5 days. 2. In case of Ansakis spp. and Toxocara canis, enzymatic activity of these proteinases was considerably inhibited by Leupeptin and EDTA. For maximum enzymatic activity of above proteinases, it was required that cysteine residue of enzyme should be protected. And it was suggested that metallo type was contained in enzyme active site. Proteinase of Trichinella spiralis contained metallo type also. 3. Although partial purification was performed in Ansakis spp. and Toxocara canis, proteins maintaining enzymatic activity were identified as a circulating antigen. From SDS-PAGE and immunoblot, 25 kDa was presented in Ansakis spp.. Specific antigen of Toxocara cains was 110 kDa protein fraction. 55 and 42 kDa proteins were reacted with normal serum. Trichinella spiralis 60 kDa protein fraction was successfully purified from excretory materials in culture. As a result of immune-reaction with Trichinella spiralis infected serum, highly purified 60 kDa protein was maintained antigenicity until final purification step.

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Effect of Pig Slurry Fertigation on Soil Chemical Properties and Yield of Tomato (Lycopersicon esculentum Mill.) (돈분 액비 관비가 토마토의 수량 및 토양화학성에 미치는 영향)

  • Park, Jin-Myeon;Lim, Tae-Jun;Kang, Seok-Boem;Lee, In-Bok;Kang, Yun-Im
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.610-615
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    • 2010
  • This study was conducted to evaluate fertigation effects of pig slurry (PS) and chemical fertilizer (CF) in tomato by analyzing the growth and yield, nutrient content and uptake, nutrient use efficiency, and soil characteristics in greenhouse cultivation. The treatments compared were; no-fertilizer, two different levels of PS (26 mg $L^{-1}$ and 52 mg $L^{-1}$), and a control treatment of chemical fertilizer. There was no significant difference in growth and yield between PS and CF treatments. however, yield reduction was observed in PS 26 mg $L^{-1}$ treatment. The N-utilization efficiency in CF treatment was similar to that of PS 52 mg $L^{-1}$ treatment. Nutrient utilization efficiency decreased in order of potassium (K), nitrogen (N), phosphate (P) with 29.2~43.3% in K, 15.8~36.7% in N, and 3.0~6.3% in P. In soil chemical characteristics, soil pH in PS treatment was higher than in CF treatment. In contrast, nitrate content in soil was higher in CF treatment than in PS treatment. The content of exchangeable K in soil was higher in PS and CF 52 mg $L^{-1}$ treatments. There was no significant difference in exchangeable Ca and Mg among those treatments. Therefore, it can be concluded that chemical fertilizers can be substituted by PS based on soil chemical analysis in tomato fertigation culture.