• Title/Summary/Keyword: propolis

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Translation Inhibition Activity and Antifungal Activity of Korean Propolis (프로폴리스의 단백질합성저해활성 및 항진균활성)

  • Goh, Ah-Ra;Choi, Kap-Seong;Choi, Sang-Ki
    • Microbiology and Biotechnology Letters
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    • v.38 no.1
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    • pp.64-69
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    • 2010
  • It has been known that propolis possesses anti-infective, anti-inflammatory, and anti-oxidative properties. Although antifungal activity of Propolis has already been demonstrated, very few studies has been conducted for action mechanism and its spectrum on fungi. We found that ethanol extract of propolis (EEP) inhibited in vitro translation. Since we also observed the growth inhibition of pathogenic fungi and anti-oxidative properties preliminarily, we try to see where those properties come from. Therefore we extracted the EEP further with chloroform, ethyl acetate and butanol. When their fractions were examined for the growth inhibition of Candida albicans, Saccharomyces cerevisiae, Candida glabrata, Candida lusitaniae, Cryptococcos neoformans, chloroform fraction exhibited the highest anti-fungal as well as anti-oxidative properties. Similarly the chloroform fraction showed highest translation-inhibiting activities among the various Propolis fractions. These data indicate that those properties might come from similar compounds.

Effects of Propolis Addition on Quality Characteristics of Oriental Medicinal Seasoning Pork (Propolis 첨가가 한방양념돼지고기의 품질특성에 미치는 영향)

  • Han, Gwi-Jeong;Shin, Dong-Sun;Kim, Jin-Sook;Cho, Yong-Sik;Jeong, Kyoung-Soon
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.75-81
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    • 2006
  • Effects of medicinal herbs and bee propolis on pork quality were assessed using formulae A (control), B (boiling water extraction with propolis), and C (boiling water extraction without propolis). Seasoning sauce was made with medicinal herb, spices, total flavonoid, and soy sauce with/without 0.4% propolis (21.8 mg/g), mixed with pork, and stored at low temperature $(4{\pm}1^{\circ}C)$ for 20 days. In formula B peroxide value and thiobarbituric acid content increased with increasing storage period. Formula B showed highest Volatile Basic Nitrogen (VBN) and lowest microbial counts, followed by formulae C and A, receiving higher scores for taste and fragrance.

Analysis of Nutritional Composition and Phenolic Compound in Propolis Collected from Falseacacia and Chestnut Tree in Korea (국내산 아까시나무와 밤나무 유래 propolis의 영양성분 및 페놀성 화합물 분석)

  • Song, Hyo-Nam;Gil, Bog-Im
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.546-551
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    • 2002
  • Nutritional composition and phenolic compounds of raw propolis collected from falseacacia (Robinia pseudoacacia L.) and chestnut tree (Castanea crenata), and their 70% ethanol extracts of propolis (EEP) were analyzed. Propolis had high crude lipid content, but no significant differences in general compositions in terms of collection area and plant origins. Mineral contents varied greatly depending on the plant origins, with falseacacia propolis showing the highest mineral content. Sixteen amino acids were analyzed, among which aspartic acid content was the highest at $328.4{\sim}410.6\;mg%$ and methionine the lowest at $0{\sim}21.1\;mg%$. Extraction yield for EEP was relatively high at $64.2{\sim}81.9%$, and total polyphenol and flavonoid contents were $13.9{\sim}23.7$ and $8.6{\sim}10.8%$, respectively. HPTLC and HPLC analysis on the phenolic compounds revealed the overall chromatographic patterns were almost equal, showing similar polyphenol compositions between the propolis. About 16 peaks were identified by HPLC analysis, among which 6 peaks of p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid, and chrysin were identified.

Evaluation of Propolis Productivity among Honey Bee Lines to Select Superior Lines (프로폴리스 생산성 우수 꿀벌 계통 선발을 위한 꿀벌 계통별 프로폴리스 생산성 평가)

  • Oh, Hyun-Rim;Kim, Hye-Kyung;Lee, Myeong-Lyeol;Lee, Man-young;Kim, Dong Won;Woo, Soon-Ok;Kang, Ah Rang;Choi, Yong-Soo
    • Journal of Apiculture
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    • v.34 no.1
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    • pp.1-6
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    • 2019
  • Honey bees use propolis to defend against invaders and pathogens. Propolis, a resinous mixture, has been widely used for its antibacterial, antifungal and anti-inflammatory properties. This research was carried out to evaluate the propolis productivity in Apis mellifera lines to select superior honeybee lines. For the study, which were collected from three regions in Korea were evaluated for the productivity of propolis. The results showed that, the average propolis per colony was highest in the line P2 (10.1 g/hive), which was inbed line derived from isolated region, compared to other lines. We also measured brood development one the basis of population size of eggs, brood, and adult bees to further probe for relation between population size and propolis yield, in that larger population generally have more foragers. Also, adult population expended more greatly in the line P2 compared to that in the control.

Antioxidant activity and anti-tumor immunity by Propolis in mice

  • Choi, In-Sook;Itokawa, Yuka;Maenaka, Toshihiro;Yamashita, Takenori;Mitsumoto, Morihide;Tano, Kaoru;Kondo, Hiroyo;Ishida, Torao;Nakamura, Takashi;Saito, Kiyoto;Terai, Kaoru;Monzen, Hajime;Oshima, Masami;Takeuchi, Tetsuo;Mituhana, Yuicti;Bamen, Kenichi;Ahn, Kyoo-Seok;Gu, Yeun-Hwa
    • Advances in Traditional Medicine
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    • v.5 no.2
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    • pp.100-109
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    • 2005
  • In South America, natural products with unknown drug effects are used as folk remedies and for preventive medicine. Among South American natural products, we directed our attention to Propolis, which have been known as medicinal plants, and examined the mechanisms by which these substances affect antioxidant activity, anti-tumor activity and immunoresponse. When the antioxidant activities of Propolis were examined by the DPPH and Rhoudan iron methods, since Propolis contains high levels of flavonoids, it is thought that flavonoids may be responsible for the antioxidant activity in this study. In the examination of immunoenhancement activity, we measured lymphocyte versus polymorphonuclear leukocyte ratios (L/P activity). The number of lymphocytes was significantly increased in groups treated with Proplolis. Specifically, slightly high levels of $IFN-{\gamma}$ were measured in mice bearing the S-180 carcinoma, after administration of Propolis. This strongly suggests that cellular immunity is especially activated by treatment with Propolis, because production of $IFN-{\alpha}$ is limited to the T cells and NK cells stimulated by mitogen and sensitized antigen. $TNF-{\alpha}$ shows a different extent and mechanism of action depending on the target cells. When $TNF-{\alpha}$ was measured in mice bearing the S-180 carcinoma, mice treated with Propolis showed slightly higher $TNF-{\alpha}$ levels as compared to the control group. This suggests that activated macrophages produce $TNF-{\alpha}$ in mice treated with Prapolis, since activated macrophages and lymphocytes are the source of most $TNF-{\alpha}$. When anti-tumor action was examined using two kinds of sarcoma (Ehrlich solid carcinoma and Sarcoma-180 carcinoma), tumor-suppressive ratios after treatment with Propolis was 29.1%. When Sarcoma-180 solid carcinoma was used, tumor-suppressive ratios were 62%. Thus, Propolis showed strong anti-tumor activity against two kinds of solid carcinoma. Taken altogether, this strongly suggests that Propolis enhances original functions of macrophages and NK cells, and as a result, secondarily enhances the immune reaction and suppresses tumor growth.

Antioxidant Activities of Ethanol and Water Extracts from Propolis (프로폴리스 에탄올 및 물 추출물의 항산화 활성)

  • Jeong, Chang-Ho;Shin, Chang-Sik;Bae, Young-Il;Shim, Ki-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.12
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    • pp.1725-1730
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    • 2010
  • To obtain basic information on the potential use of propolis as a raw material in functional food, proximate composition, total phenolics content and antioxidant activities of different propolis extracts in Korea were investigated. Propolis had the highest level of crude fat and the lowest level of crude fiber. The total phenolics content of ethanol and water extract of propolis from Geochang (GEE and GWE), ethanol and water extract of propolis from Jeju (JEE and JWE) were 184.17, 316.19, 204.33 and 47.83 mg gallic acid equivalent/g, respectively. GWE contained relatively higher levels of total phenolics than the other extracts. The antioxidant potential of the extracts was assessed by different in vitro assays such as DPPH, ABTS, reducing power, ferric reducing/antioxidant power (FRAP) and peroxidation inhibiting activities through linoleic acid emulsion system. DPPH and ABTS radical scavenging activities of all the extracts were dose dependent. The GWE exhibited the best performance in reducing power, FRAP, and lipid peroxidation using ferric thiocyanate (FTC) assay. These results demonstrated that GWE has excellent antioxidant activities and thus it has great potential as a raw material for functional food.

Stability Traits of Probiotics Isolated from Korean on Spices and Propolis (향신료와 프로폴리스에 대한 한국형 유산균의 안정성)

  • Lee, Do Kyung;Park, Jae Eun;Kim, Kyung Tae;Do, Myung Jin;Chung, Myung Jun;Lee, Gwa Soo;Kim, Jin Eung;Ha, Nam Joo
    • Korean Journal of Microbiology
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    • v.50 no.3
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    • pp.216-222
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    • 2014
  • their survival rates could be affected by various factors such as diet, stress, senescence, and antibiotics. This study was performed to evaluate the influence of various spices (garlic, ginger, scallion, onion, Chungyang red pepper, and red pepper) which have antimicrobial properties and used frequently in Korean diet, and propolis on probiotics isolated from Koreans. As a result, most Korean probiotic strains were resistant to all spices tested and propolis, and the growth rates of some Korean probiotic strains (Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus, Streptococcus thermophiles) were even increased by specific spices or propolis. But on the other hand, the growth rates of most of european probiotic strains were reduced by various spices or propolis, and the growth rates of a few european probiotic strains (L. helveticus, S. thermophiles) were greatly decreased in the presence of specific spices. Likewise, all commercial probiotic products including Korean probiotic strains were rarely affected by spices tested. However, european probiotic product tended to be greatly reduced by garlic, onion, scallion, and propolis. Therefore, these results indicate that probiotic strains isolated from Korean have the strong viability and resistance to various spices with antimicrobial properties, so that they might be appropriate for Korean intestine.

Development of Meat Seasoning Sauce Using Propolis (Propolis를 이용한 육류양념소스 개발)

  • Han Gwi-Jeong;Shin Doung-Sun;Kim Jin-Sook;Cho Yong-Sik;Jeong Kyoung-Soon
    • Korean journal of food and cookery science
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    • v.21 no.6 s.90
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    • pp.888-894
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    • 2005
  • We investigated the development of a new seasoning sauce using port, medicinal herbs and bee's propolis on the basis scientific experiment. We divided the sample manufacturing conditions of medicinal herbs into the following four formulae A (control), B (boiling water extraction, with added propolis), C (boiling water extraction, without propolis) and D (pressure extraction, without propolis). There was little difference in pH, titrable acidity, sodium chloride and sugar composition among the four formulae. The color value, was not changeable, despite being maintained in temperature storage (at $4^{\circ}C\;{\pm}2$) for 60 days. The order was formulae B, C, A and D. In particular, formulae B with added medicinal herbs and propolis showed greater change in oxidation than the other formulae, but lesser change in pH. A comparison of commercial and sample products, indicated only a small change in physicochemical characteristics. However, the sample products showed much superior sensory characteristics, than commercial products.

Radioprotective Effect of Propolis on the Blood Corpuscle of a Mouse by SEM after X-irradiation on the Whole Body (전신 X-선 조사 후 마우스의 대동맥 혈구에서 SEM을 이용한 프로폴리스의 방사선방어효과 연구)

  • Ji, Tae-Jeong
    • Journal of radiological science and technology
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    • v.31 no.1
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    • pp.49-54
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    • 2008
  • After x-ray 5Gy radiation on the whole body of a mouse using a linear accelerator, its leucocyte, erythrocyte, and platelet were observed by SEM. Also, after injecting propolis into the abdominal cavity, the radio-protective effect of blood corpuscles was studied. The observation of micromorphology in blood corpuscles revealed that the number of leukocyte, erythrocyte, and thrombocyte decreased in the experimental group and the lump got together in blood corpuscles after 10 and 20 days. In RBC, crack or break on the surface and poikilosperocytes were observed. In the irradiation group, the size of leucocytes was smaller than that in control group and the number of villus at the verge substantially decreased. The blood corpuscles in the propolis group, however, had the similar results to control group.

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Effects of Propolis on the Ovalbumin-Induced Allergic Asthma in Mice (Propolis 투여가 Ovalbumin으로 유발된 마우스의 알러지성 천식에 미치는 영향)

  • Byun, Ji-Hwan;Jo, Eun-Hee;Mendgerel, Mendgerel;Park, Min-Cheol
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.24 no.1
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    • pp.36-44
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    • 2011
  • Objective : Propolis (PP) has been used in oriental medicine. PP has various biological activities. However, its immunoregulatory and anti-inflammatory activities have not been well studied. In this study, I investigated these activities of PP by using ovalbumin-induced allergic asthma in mice. Methods : To examine the effect of PP on allergic asthma, mice were sensitized with $100{\mu}g$ of OVA and 1mg of aluminum potasssium sulfate (Alum; Sigma) intraperitoneally on day 0 and 7. On day 14, mice were challenged on consecutive 3 days with 5% OVA and AHR was assessed 24 hours after the last challenge. To examine severity of AHR, I examined the population of eosinophiles and T cells in spleen and lung and cytokine production in T cells. Futhermore, I examined histological changes during the OVA-induced allergic asthma. Results and Conclusion : PP reduced the population of eosinophil and CD4+ T cells on the OVA-induced AHR mice model. PP also inhibited IL-4 production but increased INF-g production in T cells. These results suggest that PP may be beneficial material for allergic asthma.