• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1162-1169
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• 2023

16S rRNA short amplicon sequencing-based microbiota profiling has been thought of and suggested as a feasible method to assess food safety. However, even if a comprehensive microbial information can be obtained by microbiota profiling, it would not be necessarily sufficient for all circumstances. To prove this, the feasibility of the most widely used V3-V4 amplicon sequencing method for food safety assessment was examined here. We designed a pathogen (Vibrio parahaemolyticus) contamination and/or V. parahaemolyticus-specific phage treatment model of raw oysters under improper storage temperature and monitored their microbial structure changes. The samples stored at refrigerator temperature (negative control, NC) and those that were stored at room temperature without any treatment (no treatment, NT) were included as control groups. The profiling results revealed that no statistical difference exists between the NT group and the pathogen spiked- and/or phage treated-groups even when the bacterial composition was compared at the possible lowest-rank taxa, family/genus level. In the beta-diversity analysis, all the samples except the NC group formed one distinct cluster. Notably, the samples with pathogen and/or phage addition did not form each cluster even though the enumerated number of V. parahaemolyticus in those samples were extremely different. These discrepant results indicate that the feasibility of 16S rRNA short amplicon sequencing should not be overgeneralized in microbiological safety assessment of food samples, such as raw oyster.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.97.2-97.2
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• 2010

유리기판을 이용하여 제조되는 미세 결정질 실리콘 박막 태양전지에서 진성 반도체층(i ${\mu}c$-Si:H layer, i층)은 태양전지의 광 흡수층으로 사용되기 때문에 그 특성은 매우 중요하다. 특히, i층의 결정분율 변화는 태양광의 흡수파장 및 효율을 결정하여 준다. 본 연구에서는 i층 증착시 $SiH_4$ 가스의 농도 변화 및 $SiH_4$ 가스 profiling을 통하여 i층의 결정분율을 변화하였으며, 이에 따른 i층 단위박막과 미세결정질 태양전지 특성변화를 분석 하였다. i층의 $SiH_4$ 가스 농도가 증가함에 따라 i층 단위박막의 결정분율은 증가하였으며, 전기 전도도는 감소하였다. 또한, i층의 $SiH_4$ 가스 농도를 점차 증가하며 profiling 하여 증착한 박막은 동일 가스 농도로 증착한 박막보다 전기 전도도가 감소하였고, 증착속도는 증가하였다. 이와 같은 다양한 i층들은 'Raman spectroscopy'를 통하여 결정분율 변화를 측정하였다. 또한, 이 박막들을 광 흡수층으로 사용하는 미세결정질 태양전지를 제조하고, 태양전지의 효율, 양자효율, 암전류 특성들을 단위박막 특성과 연계하여 분석하였다.

• Steel and Composite Structures
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• 제34권3호
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• pp.333-345
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• 2020

In the past, many efficient profiles have been developed for cold-formed steel (CFS) members by judicious intermediate stiffening of the cross-sections, and they have shown improved structural performance over conventional CFS sections. Most of this research work was based on numerical modelling, thus lacking any experimental evidence of the efficiency of these sections. To fulfill this requirement, experimental studies were conducted in this study, on efficient intermediately stiffened CFS sections in flexure, which will result in easy and simple fabrication. Two series of built-up sections, open sections (OS) and box sections (BS), were fabricated and tested under four-point loading with same cross-sectional area. Test strengths, modes of failure, deformed shapes, load vs. mid-span displacements and geometric imperfections were measured and reported. The design strengths were quantified using North American Standards and Indian Standards for cold-formed steel structures. This study confirmed that efficient profiling of CFS sections can improve both the strength and stiffness performance by up to 90%. Closed sections showed better strength performance whereas open sections showed better stiffness performance.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.135-139
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• 1991

Tridecanedioic acid (DC-13), starting material of the valuable musk ethylene brassylate, was obtained from n-tridecane by the Candida tropicalis mutant. The mutants were first obtained from primary screening step using the selective medium and then solid phase extraction sampling method was used for the selective isolation of organic acids from the cultured media of mutants. The resulting acids were directly converted to volatile tert-butyldimethyl silyl delivatives, which were then analyzed by gas chromatography. The efficient GC profiling method was used for the rapid identification of the mutant producing DC-13 in large quantity, and for the optimization of the culture conditions of mutant. The optimal culture conditions were found as follows: pH 8.0, 30$^{\circ}C$, 250rpm, 48hour of culture and $(NH_4)_2HPO_4$ as nitrogen source.

• 한국자기공명학회논문지
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• 제15권1호
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• pp.54-68
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• 2011

$^1H$ nuclear magnetic resonance (NMR) spectroscopy of biological samples has been proven to be an effective and nondestructive approach to probe drug toxicity within an organism. In this study, ketoprofen toxicity was investigated using $^1H$-NMR spectroscopy coupled with multivariate statistical analysis. Histopathologic test of ketoprofen-induced acute gastrointestinal damage in rats demonstrated a significant dose-dependent effect. Furthermore, principal component analysis (PCA) derived from $^1H$-NMR spectra of urinary samples showed clear separation between the vehicle-treated control and ketoprofen-treated groups. Moreover, PCA derived from endogenous metabolite concentrations through targeted profiling revealed a dose-dependent metabolic shift between the vehicle-treated control, low-dose ketoprofen-treated (10 mg/kg body weight), and high-dose ketoprofen-treated (50 mg/kg) groups coinciding with their gastric damage scores after ketoprofen administration. The resultant metabolic profiles demonstrated that the ketoprofen-induced gastric damage exhibited energy metabolism perturbations that increased urinary levels of citrate, cis-aconitate, succinate, and phosphocreatine. In addition, ketoprofen administration induced an enhancement of xenobiotic activity in fatty oxidation, which caused increase levels of N-isovalerylglycine, adipate, phenylacetylglycine, dimethylamine, betaine, hippurate, 3-indoxylsulfate, N,N-dimethylglycine, trimethyl-N-oxide, and glycine. These findings demonstrate that $^1H$-NMR-based urinary metabolic profiling can be used for noninvasive and rapid way to diagnose adverse drug effects and is suitable for explaining the possible biological pathways perturbed by nonsteroidal anti-inflammatory drug toxicity.

• 정보관리학회지
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• 제31권3호
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• pp.293-311
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• 2014

이 연구에서는 계량정보학적 기법을 사용하여 국내 트위터 관련 연구의 동향을 분석하고자 하였다. 이를 위해 KCI에서 검색된 2009년부터 2014년 4월까지의 트위터 관련 논문 539편에서 제목, 초록, 키워드를 추출하여 분석 자료로 삼았다. 프로파일링 기법을 이용해 트위터 관련 연구가 수행된 학문 분야와 저널을 분석하였고, 동시출현단어 분석을 통해 트위터 관련 연구의 세부 주제 영역을 파악하였다. 그 결과, 국내 트위터 관련 연구는 53개 학문분야에서 다양하게 다루어지고 있으며 핵심 분야는 신문방송학, 경영학, 컴퓨터학 분야로 나타났다. 세부 주제로는 선거를 비롯한 정치 관련 이슈가 가장 많이 다루어졌으며, 기업/구매 관련 이슈도 활발히 연구되었음을 확인할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제35권3호
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• pp.845-850
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• 2014

The rapid development of shotgun proteomics is paving the way for extensive proteome profiling, while providing extensive information on various post translational modifications (PTMs) that occur to a proteome of interest. For example, the current phosphoproteomic methods can yield more than 10,000 phosphopeptides identified from a proteome sample. Despite these developments, it remains a challenging issue to pinpoint the true phosphorylation sites, especially when multiple sites are possible for phosphorylation in the peptides. We developed the Phospho-UMC filter, which is a simple method of localizing the site of phosphorylation using unique mass classes (UMCs) information to differentiate phosphopeptides with different phosphorylation sites and increase the confidence in phosphorylation site localization. The method was applied to large scale phosphopeptide profiling data and was demonstrated to be effective in the reducing ambiguity associated with the tandem mass spectrometric data analysis of phosphopeptides.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.338-345
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• 2005

Recently, surface-wave methods have been widely used for site investigation due to economic advantage and improved reliability. Specially, the Spectral-Analysis-of-Surface-Wave (SASW) method has been used to evaluate soil properties in geotechnical engineering. In determination of subgrade stiffness by SASW measurements, only the vertical Rayleigh waves have been used. This study proposed a framework to determine shear-wave velocity profiles by using vertical and horizontal Rayleigh waves and Love wave all together. In addition, the Common-Array-Profiling(CAP) SASW method was employed, which subgrade stiffness of profile the local material under two fixed receivers. The procedure proposed in this study was verified by comparing the shear-wave velocity profiles with the shear-wave velocity profiles of downhole testing at two geotechnical sites.

• 인터넷정보학회논문지
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• 제21권2호
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• pp.91-97
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• 2020

최근 국방, 안보, 외교 분야 관련자를 대상으로 하는 피싱 공격이 급증하고 있다. 특히 해킹 공격조직 Kimsuky는 2013년 이후 피싱 공격을 통해 공공기관의 주요 정보 수집을 위한 활동을 하고 있다. 본 논문에서는 피싱 메일 공격조직에 대한 프로파일링 분석을 수행하였다. 이를 위해 피싱 메일 공격의 유형을 분류하고 해킹 공격조직의 공격방식에 대한 분석을 하였다. 상세한 프로파일링 분석을 통해 공격조직의 목적을 추정하고 대응방안을 제시하였다.

• 정보보호학회논문지
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• 제23권5호
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• pp.939-946
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• 2013

본 논문에서는 IEC 61850 기반 자동화 변전소 네트워크에서의 이상 징후 탐지를 위한 MMS/GOOSE 패킷 정상행위 프로파일링 방법을 제안한다. 기존에 주로 사용되고 있는 시그니처(signature) 기반의 보안 솔루션은 제로데이(zero-day) 취약점을 이용한 APT 공격에 취약에 취약할 수밖에 없다. 최근 제어시스템 환경에서의 이상 탐지(anomaly detection) 연구가 이뤄지고 있지만, 아직까지 IEC 61850 변전소 환경에서의 이상 탐지에 대한 연구는 잘 알려져 있지 않다. 제안하는 기법은 MMS/GOOSE 패킷에 대한 3가지 전처리(3-phase preprocessing) 방법과 one-class SVM 알고리즘을 이용한 정상 행위 모델링 방법을 포함한다. 본 논문에서 제시하는 방법은 IEC 61850 변전소 네트워크에 대한 APT 공격 대응 솔루션으로 활용될 것을 기대한다.