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Quality Improvement of Rainbow Trout with Pigments and Enzymatic Hydrolysates of Ascidian (Halocynthia roretzi) Tunic 1. Chemical Specificity of Ascidian Tunic and Its Hydrolysates (우렁쉥이 껍질의 색소 및 효소 가수분해물을 이용한 무지개 송어의 품질 향상 1. 우렁쉥이 껍질 및 효소 가수분해물의 화학적 특성)

  • CHOI Byeong-Dae;KANG Seok-Joong;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.3
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    • pp.345-356
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    • 1996
  • Properties of enzymatic hydrolysates from ascidian tunic were assessed on supernatant ratio, solid yields and solid concentration. The concentartion of solid and yields in the extracts were increased as the enzyme concentration raised from $100\;{\mu}l\;to\;1000{\mu}l$ during the extraction period. The optima concentration and reaction time of each enzyme on digestion were $400\;{\mu}l$ 60 minutes, through treated with Duncan's multiple test. The percent of yields of solid, protein and carotenoids for 60 minutes extraction at $400\;{\mu}l$ were $32.32\%,\;1.34\%\;and\;74.60\;mg\%$, respectively, in Viscozyme systems. The extracts were composed with many kinds of carbohydrates such as arabinose, ribose, xylose, galactose, glucose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine. Aspartic and glutamic arid were noted as predominant amino acids in all parts. Amino acid profiles of various ascidian tunic part were similiar to each other, but most of essential amino acids content of inter coat was higher than that of root and tunic (body). About sixty six fatty acids components were observed, and their distribution among neutral and polar lipids was compared. The main fatty acids were found to be 14:0, 16:0, 16:1n7, 18:0, 18:1n9, 18:1n7, 18:2n6, 20:5n3, and 22:6n3.

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Regeneration Processes of Nutrients in the Polar Front Area of the East Sea II. Distribution of Particulate Organic Carbon and Nitrogen in Winter, 1995 (동해 극전선의 영양염류 순환과정 II. 1995년 동계 입자태 유기탄소 및 유기질소의 분포)

  • YANG Han-Soeb;MOON Chang-Ho;OH Seok-Jin;LEE Haeng-Pil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.442-450
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    • 1997
  • The chemical properties of water masses were investigated at 33 stations of the southeastern last Sea in February, 1995 on board R/V Tam-Yang. The water masses were not clearly distinguished due to the vortical mixing in winter. However, on the basis of the T-S and $T-O_2$ diagrams, water masses in the study area were divided into five groups (Type I, Type II, Type III, Type IV, Type V). (1) $>9.0^{\circ}C,\;>34.35\;psu,\;5.08\~5.60m\ell/\ell$ at Type I, (2) $6.0\~9.0^{\circ}C,\;34.15\~34.35\;psu,\;5.60\~5.90\;m\ell/\ell$ at Type II, (3) $4.0\~6.0^{\circ}C,\;34.00\~34.15\;psu,\;>5.90m\ell/\ell$ at Type III, (4) $1.5\~4.0^{\circ}C,\;34.00\~34.05\;psu,\;5.40\~5.90\;m\ell/\ell$ at Type IV, (5) $<1.5^{\circ}C,\;34.05\~34.07\;psu,\;4.80\~5.40\;m\ell/\ell$ at Type V. In the vertical profiles of nutrients, the concentrations were very low in the surface layer and increased rapidly with depth. The highest concentrations occurred in Type IV, while the concentrations in Type I were the lowest. The N/P ratios were less than Redfield ratio, indicating that nitrogenous nutrients were the limiting factor tor phytoplankton growth. The concentrations of POC and PON were in the range of $0.49\~20.03\;{\mu}g-at/\ell\;and\;0.09\~5.34\;{\mu}g-at/\ell$, respectively. The relatively high concentration occured in the surface layer of inner shore, showing that the concentration at each water mass followed the order Type I > Type II > Type III > Type IV > Type V, respectively. The C:N ratio in particulate organic matter was lower than the values reported in other region due to relatively high concentrations of PON in the study area. Relatively high ratios of POC to chlorophyll $\alpha$ during the study periods indicate that non-living detritus comparised most of the POC in the study area.

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Effects of Vegetable Sprout Power Mixture on Lipid Metabolism in Rats Fed High Fat Diet (새싹채소 혼합분말이 고지방 식이를 급여한 흰쥐의 지질대사에 미치는 영향)

  • Lee, Jae-Joon;Lee, Yu-Mi;Shin, Hyoung-Duck;Jeong, Young-Sim;Lee, Myung-Yul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.8
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    • pp.965-974
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    • 2007
  • This study was performed to investigate the effects of vegetable sprout powder on serum and adipose tissue lipid metabolism in rats fed high-fat diet for 4 weeks for induction hyperlipidemic model rat. Weight-matched male Sprague-Dawley rats were assigned to five groups according to dietary fat level (10% or 20% of diet wt.) and mixture of vegetable sprout powder levels (5% or 10% 10% or 20% of diet wt.). Vegetable sprout powder was the mixture of same amounts of dried barley, broccoli, rapeseed, alfalfa, radish, mustard, buckwheat and brussels sprouts. Experimental groups were normal fat diet with 5% cellulose (NF-C), high fat diet without fiber (HF-N), high fat diet with 5% cellulose (HF-C), HF-C diet with 5% vegetable sprout powder (HF-CSL), and HF-C diet with 10% vegetable sprout powder (HF-CSH). The body weight of HF-N group increased 16% compared with the NF-C group, while it was decreased by 15% and 22% for HF-CSL group and HF-CSH group, respectively. Fat mass and fat cell size of adipose tissue were lower in HF-CSL group and HF-CSH group compared with HF-C group, and lower in HF-CSH group compared with HF-CSL group. Serum triglyceride, total cholesterol and LDL-cholesterol contents were markedly decreased by vegetable sprout powder containing diet, while the serum HDL-cholesterol and phospholipid contents were higher in vegetable sprout powder containing diet in a dose-dependent manner. Leptin and insulin levels in serum showed a decrease in HF-CSH group. Significantly increased contents of triglyceride, total cholesterol, LDL-cholesterol, leptin and insulin in the serum of HF-N group were returned to normal or even below normal levels by feeding 10% vegetable sprout powder diet. The increased activities of NADP-malate dehydrogenase (ME), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) and lipoprotein lipase (LPL) in adiposetissue by HF-N group were decreased to the activity of normal fat group by feeding vegetable sprout powder in a dose-dependant manner. These results indicate that lipid metabolism in rats fed high-fat diet was suppressed by feeding vegetable sprout powder.

Submicroscopy of Forest Soils (kandiustults) Derived from Granite in Southern Part of Korea (우리나라 남부지역(南部地域) 화강암질(花崗巖質) 삼림토양(森林土壤)의 SEM과 TEM에 의한 관찰(觀察))

  • Cho, Hi Doo;An, Ki Wan
    • Journal of Korean Society of Forest Science
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    • v.90 no.5
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    • pp.608-618
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    • 2001
  • To understand the weathering processes of the soil by submicroscopic method is very important to realize the properties of the soils. In this study soil formation processes show every steps to the changes in chemical and mechanical properties and the submicroscopic characteristics of soil weathering on the profiles of forest soils derived from granite in southern part of Korea. Fecal pellets(SEM) are given a full detail of the positive activities of the forest soil animals; mainly invertebrates in the O horizon and the E horizon. External shapes of fecal pellets have been divided into five groups : spherical, ellipsoidal, cylindrical, platy and threadlike. But doughnutlike form of fecal pellets is observed in this study. The soluble and suspended materials in the soils move downwards by percolation from the A horizon to the B or the BC horizons, and result in the illuviation cutans(SEM) on the ped surface of the lower horizon and deposited stack of kaolinite. Illuviated cutans are deposited on the ped surface even in the depth of 312cm in the BC horizon as well as the Bt horizon and comprise of fine silt, coarse clay and fine clay. A lot of halloysites are observed on the cutan surface. Halloysite formation from feldspars has been well known but a lot of hallyosite formation are observed in this study. The formation were predicted by Jackson(1962), inferred by Wada and Kakuto(1983a, b) and proved evidently by Cho and Mermut(1992a, b). This also suggests that halloysites in the soils derived from granite are formed a lot from ferruginous chlorites. The release of Fe from the chlorite structure are significant pedogenic processes and newly formed Fe oxides imparted a red color to the soils. The iron oxides particles, which are ejected and recrystalized, aggregate thickly on the edge of the ferruginous chlorites, and this indicates the release of structural Fe from weathered chlorites. Hematites and goethites are frequent in the fine clay in this soils.

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Effects of Extracts of Persimmon Leaf, Buckwheat Leaf, and Chinese Matrimony Vine Leaf on Body Fat and Lipid Metabolism in Rats (감잎, 메밀잎 및 구기자잎 추출물의 식이보충이 흰쥐의 체지방 감량과 지질대사 조절에 미치는 효과)

  • Jung, Un-Ju;Lee, Jeong-Sun;Bok, Song-Hae;Choi, Myung-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.9
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    • pp.1215-1226
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    • 2011
  • The objective of this study was to investigate the anti-obesity and lipid-lowering effects of extracts of persimmon leaf (PWE), buckwheat leaf (BWE), and chinese matrimony vine leaf (LWE) in rats fed a high-fat diet (HFD). Male Sprague-Dawley rats (n=40) were divided into four groups: HFD (35% fat, w/w), HFD (38.5% fat, w/w) supplemented with PWE (10%, w/w), BWE (10%, w/w), and LWE (10%, w/w) groups. The epididymal, perirenal, and interscapular white adipose tissue (WAT) weights as well as plasma leptin level were lowest in the LWE group. Supplementation with PWE and BWE also tended to lower the perirenal and retroperitonal WAT weights compared to the HFD control group, and there was a significant decrease in plasma leptin concentration. Furthermore, plasma triglyceride concentration, hepatic cholesterol content, and hepatic lipid droplet accumulation were significantly lower in the PWE, BWE, and LWE groups than in the HFD group. BWE supplementation markedly lowered plasma total cholesterol concentration, although there were no significant differences in plasma HDL-cholesterol concentration and ratio of HDL-cholesterol/total cholesterol among the groups. Hepatic HMG-CoA reductase activity was significantly higher in the PWE and LWE groups than in the HFD group, and hepatic ACAT was not changed by extract supplementation. However, supplementation with PWE, BWE, and LWE significantly increased fecal acidic sterol content in rats fed a HFD. These results suggest that supplementation with PWE, BWE, and LWE may be an effective anti-obesity strategy by lowering body fat weight and improving plasma and hepatic lipid profiles in HFD-fed rats.

Effects of Kimchi Lactic Acid Bacteria Lactobacillus sp. OPK2-59 with High GABA Producing Capacity on Liver Function Improvement (GABA 생성능 우수 김치 젖산균 Lactobacillus sp. OPK2-59의 간 기능 개선 효과)

  • Bae, Mi-Ok;Kim, Hye-Jin;Cha, Youn-Soo;Lee, Myung-Ki;Oh, Suk-Heung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.11
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    • pp.1499-1505
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    • 2009
  • This study investigated the effect of improved liver function in rats administered with ethanol by kimchi lactic acid bacteria with high GABA producing capacity. Sprague-Dawley male rats were divided into four groups; normal diet control (NC), ethanol control (EC), ethanol+Lactobacillus sp. OPK2-59 normal powder (EL1), ethanol+Lactobacillus sp. OPK2-59 GABA powder (EL2) and fed for 6 weeks. Analysis showed that there were no significant differences in body weight and feed consumption among the groups during the experimental period. Also, there were no significant differences in organ weight among the groups. The test results showed total cholesterol and triglyceride in the blood concentration that were increased by ethanol administration were significantly lowered in EL2 group. Liver triglyceride was also significantly lowered in the EL2 group compared with the EC group. Serum GOT and GPT, and liver GOT levels were significantly lower in the EL2 group compared with the EC group. Serum ethanol concentration was lower in the EL1 and EL2 groups compared with the EC group. SOD activities in liver were significantly increased in the EL1 and EL2 groups compared with the EC group. These results suggest that Lactobacillus sp. OPK2-59 GABA powder improves lipid and enzyme profiles of rats administered with ethanol.

Comparative Study of Total Acidity Content of Makgeolli with Pre-Treatment and Analysis Methods (전처리과정과 분석방법에 따른 막걸리의 총산 함량 비교)

  • Kim, Ye Seul;Shin, Kwang Seup;Lee, Jeung Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.600-607
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    • 2017
  • The total acidity (TA) of Makgeolli was affected by suspended solids and $CO_2$ produced during the fermentation process. Nine Makgeollis (four sterilized and five unsterilized Makgeollis) were collected in the market, and their TAs were compared before and after filtration and $CO_2$ removal. TAs of sterilized Makgeollis were 0.379~0.477%, which significantly decreased to 0.167~0.225% after filtration and 0.132~0.170% after $CO_2$ removal (P<0.05). TAs of unsterilized Makgeollis were 0.412~0.467% and decreased to 0.157~0.365% after filtration and 0.143~0.280% after $CO_2$ removal (P<0.05). TAs of Makgeollis were compared by three methods using different indicators. The TAs of sterilized and unsterilized Makgeollis were 0.105~0.123% and 0.105~0.200%, respectively, by bromthymol blue+neutral red (light green), 0.129~0.154% and 0.130~0.255%, respectively, by phenolphthalein (faint pink), and 0.120~0.146% and 0.130~0.232%, respectively, by bromthymol (blue). Nowadays, Makgeolli is commercialized with various distinct colors, and thus it is important to select appropriate indicators for proper titration endpoint identification for TA measurement. The compositions of organic acids profiles varied depending on sterilized or unsterilized Makgeollis, in which oxalic acid (0.108~0.329 mg/mL), malic acid (ND to 0.134 mg/mL), lactic acid (0.127~0.776 mg/mL), and citric acid (ND to 1.159 mg/mL) were found, and lactic acid was in unsterilized more than sterilized Makgeollis.

Properties of Blood Pressure and Routine Laboratory Test Results by the Status of Smoking and Alcohol Intakes in Male Workers (일부 산업장 남성근로자들의 흡연,음주실태에 따른 혈압 및 혈액검사치의 특성)

  • Yoo, Chang-Kyun;Jeong, Yong-Jun
    • Korean Journal of Health Education and Promotion
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    • v.20 no.1
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    • pp.131-145
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    • 2003
  • This study was conducted to estimate the incidence and the degree of cigarette smoking and drinking among working men, and then to investigate the effects on blood pressure, various hematological indices and blood chemistry. The sample consisted of 2,287 male workers who had undertaken a general health check-up during the two-year period from January, 2000 to December, 2001. Such factors as blood pressure, blood glucose, lipid profiles, and liver function tests were determined and then analyzed with respect to the subjects smoking and/or drinking status. The major findings from this study are: 1. The drinking and smoking status have shown that 52.7% of participants were in the habit of both drinking and smoking while 11.6% were not associated with either. On the other hand 25.4% were involved only in drinking and 10.2% only in smoking. In the group smoking over 21 cigarettes per day over 30, the age group occupied the largest proportion at 20.1%. 2. Regarding the relationship between smoking and/or drinking status, and blood pressure, hematology and blood chemistry, the smoking and/or drinking group had significantly higher levels of blood pressure, both systolic and diastolic, Hb & Hct, TG, LDL-C, SGOT, and ${\gamma}$-GTP, than the non-smoking and/or non-drinking group. But HDL-C was significantly lower in the smoking group and significantly higher in the drinking group than the non-smoking/non-drinking group. 3. Regarding amount smoked, a larger number of cigarettes per day was significantly associated with the higher levels of blood pressure, systolic and diatolic, TG, TC, LDL-C, Hb, Hct, and ${\gamma}$-GTP. As for the amount druck, an increasing amount of alcohol intake was associated with rising levels of blood pressure, systolic and diatolic, TG, TC, LDL-C, HDL-C, Hb, SGOT, and ${\gamma}$-GTP. 4. Regarding the correlation among all the variables stated above, the smoking and drinking amount was shown to be in the positive correlation with blood pressure, both systolic and diastolic, TG, TC, Hb, and ${\gamma}$-GTP. On the contrary, LDL-C and HDL-C was in a positive correlation only with the amount drunk amount, and Hct only with the amount smoked. 5. As with systolic and diastolic blood pressure, the odds ratio of the smoking group was 2.35 and 2.58 compared to the non-smoking/drinking group. whereas it was 1.47 and 1.75 in the smoking/drinking group. Concerning serum lipids, the smoking/drinking group had 1.97 times the levels of TG in the non-smoking/non-drinking group, though the smoking group had 1.55 times the levels of HDL-C in the non-smoking/non-drinking group. As with liver function test results, the drinking group had 2.50 times and the smoking/drinking group had 4.41 times the levels of ${\gamma}$-GTP in the non-smoking/drinking group. respectively. The above results revealed that smoking and alcohol intake were effected the results of blood pressure and laboratory tests. Specifically, not only the smoking/drinking group but also those only smoking or only drinking were not as desirable as the non-smoking and non-drinking group to the results of blood pressure and laboratory tests.

Roots Growth Characteristics of Zelkova serrata Makino. after Replanting in the Reclaimed Land from the Sea - On the Root Structure and Spatial Distribution of Fine Root Phytomass - (임해매립지의 느티나무 식재 이후 뿌리 생장특성 -뿌리구조 및 세근의 공간적 분포를 중심으로-)

  • Kim, Do-Gyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.35 no.5
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    • pp.46-55
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    • 2007
  • This study was carried out to analyze both the root structure and the fine root phytomass of the vertical and horizontal distribution of Zelkova serrata Makino. which was transplanted in the reclaimed land from the sea in Gwangyang, Jeonnam, South Korea. The base ground was reclaimed land from the sea. $Z_1$ of the planting ground was filled to a $100{\sim}150cm$ thickness with the improved soil instead of the reclaimed soil from the sea, $Z_2$ of the planting ground was covered to a $20{\sim}30cm$ thickness with the improved soil and $Z_3$ of the planting ground was mounded to 120cm thickness with the improved soil on the reclaimed land from the sea. In addition, $Z_4,\;Z_5\;and\;Z_6$ of the planting grounds were at the large-sized mound on the reclaimed land from the sea. $Z_4$ of the planting ground was located at the lowest level, $Z_5$ planting ground was located at the slope and $Z_6$ planting ground was located at the top of the large-sized mound. The large-sized mounds contain 3 layers, the base layer was reclaimed land from the sea and the second layer was mounded to a $200{\sim}300cm$ thickness with the desalinized soil from the sea on the base layers and the finally layers were mounded to a $80{\sim}120cm$ thickness with improved soil on the second layer. The planting grounds $Z_3,\;Z_4,\;Z_5\;and\;Z_6$ developed roots such as tap roots, lateral roots and heart roots. However, in $Z_1\;and\;Z_2$ roots development were inhibited. The fine-root phytomass of the 6 planting ground types was as follows: $113.5g\;DM/m^2$ for $Z_5$, $105.5g\;DM/m^2$ for $Z_4$, $88.3g\;DM/m^2$ for $Z_3$, $81.0g\;DM/m^2$ for $Z_6$, $73.0g\;DM/m^2$ for $Z_2$, $43.3g\;DM/m^2$ for $Z_1$. The vertical distribution of the fine root phytomass decreased from the upper to the deeper soil profiles in the 6 mound types. The fine root phytomass was $43.3{\sim}71.8%$ in a $0{\sim}20cm$ thickness of soil layer and it decreased according to the distance from the nearest trees. The root growth in the improved soil was better than in the reclaimed soil from the sea. However, root growth decreased more in the disturbed soils even though the planting grounds contained the improved soils. The retarded development of roots and the spatial distribution patterns of the fine root phytomass were closely connected to the reclaimed soil from the sea. In the disturbed soil, the soil hardness and alkalic cation($Na^+,\;K^+,\;Ca^{2+},\;Mg^{2+}$). were high and the soil water was lacking. We suggest that the construction of planting grounds and the improvement of bad soil are necessary for the proper and effective growth of landscaping plants.

Dose Distribution and Design of Dynamic Wedge Filter for 3D Conformal Radiotherapy (방사선 입체조형치료를 위한 동적쐐기여과판의 고안과 조직내 선량분포 특성)

  • 추성실
    • Progress in Medical Physics
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    • v.9 no.2
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    • pp.77-88
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    • 1998
  • Wedge shaped isodoses are desired in a number of clinical situations. Hard wedge filters have provided nominal angled isodoses with dosimetric consequences of beam hardening, increased peripheral dosing, nonidealized gradients at deep depths along with the practical consequendes of filter handling and placement problems. Dynamic wedging uses a combination of a moving collimator and changing monitor dose to achieve angled isodoses. The segmented treatment tables(STT) that monitor unit setting by every distance of moving collimator, was induced by numerical formular. The characteristics of dynamic wedge by STT compared with real dosimetry. Methods and Materials : The accelerator CLINAC 2100C/D at Yonsei Cancer Center has two photon energies (6MV and 10MV), currently with dynamic wedge angles of 15$^{\circ}$, 30$^{\circ}$, 45$^{\circ}$ and 60$^{\circ}$. The segmented treatment tables(STT) that drive the collimator in concert with a changing monitor unit are unique for field sizes ranging from 4.0cm to 20.0cm in 0.5cm steps. Transmission wedge factors were measured for each STT with an standard ion chamber. Isodose profiles, isodose curves, percentage depth dose for dynamic wedge filters were measured with film dosimetry. Dynamic wedge angle by STT was well coincident with film dosimetry. Percent depth doses were found to be closer to open field but more shallow than hard wedge filter. The wedge transmission factor were decreased by increased the wedge angle and more higher than hard wedge filters. Dynamic wedging probided more consistent gradients across the field compared with hard wedge filters. Dynamic wedging has practical and dosimetric advantages over hard filters for rapid setup and keeping from table collisions. Dynamic wedge filters are positive replacement for hard filters and introduction of dynamic conformal radiotherapy and intensity modulation radiotherapy in a future.

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