• 동의생리병리학회지
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• 제25권3호
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• pp.528-532
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• 2011

This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.

• Journal of Applied Biological Chemistry
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• 제59권4호
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• pp.317-322
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• 2016

UV exposure induces matrix metalloproteinases (MMPs) and extracellular matrix-degrading enzymes expression. We studied the protective effect of Rubus occidentalis seed against UVB-generated skin photoaging using human fibroblast cells (CCD-986sk). We used an ELISA kit to measure the supernatents of procollagen type I and MMP-1 in CCD-986sk cells after they were exposed to UVB irradiation. The CCD-986sk cells that were used with RC-E/E after the UVB irradiation caused higher levels of type I procollagen and lesser levels of MMP-1 compared with the control group. Furthermore, the RC-E/E treated group showed lesser MMP-1 levels and higher procollagen type I levels than the untreated counterpart. Therefore, it can be concluded that Rubus occidentalis seed can prevent from skin photoaging.

• 대한화장품학회지
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• 제32권2호
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• pp.117-121
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• 2006

collagen을 분해하여 광노화 과정에 매우 중요한 역할을 하는 것으로 알려져 있는 Matrix metallo proteinases (MMP)의 활성 및 저해 인자에 대해서는 지금까지 많은 연구가 진행되어 왔지만, 녹차 카테킨의 영향에 대한 연구는 epigallocatechin-3 gallate (EGCG) 이외에는 별로 알려진 것이 없다. 본 연구에서는 카테킨이 사람 섬유아세포에서의 MMP-1의 발현과 MMP-2의 활성 및 type I procollagen 생성에 미치는 영향을 조사하였다. 또한, 녹차의 대표적인 카테킨인 EGCG를 포함하여 자연적으로 존재하는 여덟개의 카테킨을 모두 사용하여 각각의 활성을 비교하였다. 그 결과, UVA에 의해서 사람 섬유아세포에서 발현되는 MMP-1에 대해 단백질의 양적인 변화는 EGCG 및 gallocatechin-3-gallate (GCG)에서 최대 57.4, 62.8% 감소되었으며, MMP-2의 활성 역시 감소되었다. 반면에, type I procollagen에 대해서는 생성 촉진능을 보였는데, 흥미롭게도 $1{\mu}M$ 이하의 저농도에서만 효능을 나타내었다. 또한 EGCG, GCG, epicatechin-gallate (ECG) 세가지 카테킨이 0.5:1.5:1.3의 비율로 조합된 경우, procollagen 합성에 가장 높은 효과를 나타내었다. 이러한 실험 결과를 통해 녹차 카테킨은 항산화능 뿐만이 아니라 자외선에 의한 MMP의 발현과 활성을 조절함으로써 콜라겐 분해를 억제함과 동시에 콜라겐 생합성을 촉진하는 것이 가능함을 확인하였다. 따라서 녹차 카테킨은 광노화의 억제 및 피부 노화 개선에 훌륭한 천연 소재로서 응용 가능할 것으로 보인다.

• 대한본초학회지
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• 제26권1호
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• pp.29-33
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• 2011

Objectives : This study was designed to investigate the collagen metabolism and tyrosinase activity of Angelicae Gigantis Radix extracts (AGR). Methods : The effect of AGR on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of AGR was measured. Results : Type I procollagen production was recovered by AGR in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by AGR and the tyrosinase activity was significantly reduced. However, the L-DOPA oxidation was not changed. Conclusion : AGR showed the anti-wrinkle effects and whitening effects in vitro. These results suggest that AGR may have potential as an anti-aging ingredient in cosmetic herbs.

• 대한약침학회지
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• 제14권1호
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• pp.51-59
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• 2011

Objectives : This study was designed to investigate the collagen metabolism and tyrosinase activity of Sipgeondaebo-tang Pharmacopuncture extracts (SP). Methods : The effect of SP on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of SP was measured as well. Results : Type I procollagen production was recovered by SP in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by SP. The tyrosinase activity was significantly reduced as well. However, the L-DOPA oxidation was not changed. Conclusion : SP showed the anti-wrinkle effects and whitening effects in vitro. These results suggest that SP may be a potential pharmacopuncture as an anti-aging pharmacopuncture treatments.

• International Journal of Oral Biology
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• 제31권3호
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• pp.93-98
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• 2006

Biosynthetic processing of fibrillar procollagens is essential for producing mature collagen monomers that polymerize into fibrils by a self-assembly process. The metalloproteinase ADAMTS-2 is the major enzyme that processes the N-propeptide of type I procollagen in the skin and also of type II and type III procollagens. Mutations in the ADAMTS-2 gene cause dermatospraxis in animals and Ehlers-Danlos syndrome VIIC in humans, both of which are characterized by the accumulation of type I pN-collagen and the formation of abnormal collagen fibrils in the skin. Despite its importance in procollagen processing, little is known about the regulation of ADAMTS-2 expression. Here, we demonstrate that ADAMTS-2 can be regulated by 1,25-dihydroxyvitamin D3, an inducer of type I procollagen synthesis. This steroid hormone induced ADAMTS-2 mRNA ${\sim}3-fold$ in MG-63 human osteosarcoma cells and MC3T3-E1 murine osteoblastic cells. This induction was dose- and time-dependent in MG-63 cells. In contrast, secreted ADAMTS-2 protein was increased only 1.4-fold with 1,25-dihydroxyvitamin D3. Finally, 1,25-dihydroxyvitamin D3 in the presence of ascorbate increased levels of secreted ADAMTS-2 1.9-fold over ascorbate treatment alone, which did not appreciably change ADAMTS-2 expression. These data indicate that the regulation of ADAMTS-2 is coupled with the synthesis of type I procollagen through 1,25-dihydroxyvitamin D3 signaling and may involve translational or posttranslational control.

• 대한한의학방제학회지
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• 제14권2호
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• pp.67-75
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• 2006

The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves. flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and $H_2O_2-induced$ cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 ${\mu}g/ml$ showed maximum cell viability on CCD-986sk cells by 129.70%. and $H_2O_2-induced$ cytotoxicity was blocked by ENL (5. 10. 20. 30. and 40 ${\mu}g/ml$) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.

• Journal of Nutrition and Health
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• 제49권6호
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• pp.429-436
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• 2016

본 연구에서는 피부의 표피와 진피에 분포하는 HaCaT 세포와 HDF 세포를 이용하여 항산화효과가 우수한 두릅순 추출물의 처리가 UVB에 의한 피부광노화를 억제할 수 있는지 알아보기 위하여 피부 염증반응과 관련한 사이토카인과 피부의 주요 구성 단백질인 collagen에 영향을 미칠 수 있는 MMP-1, type-I procollagen, TRPV-1 등의 단백질 발현에 미치는 영향을 분석하였다. HaCaT에 두릅순 추출물을 24시간 전처리한 경우 UVB ($55 mJ/cm^2$) 노출로 인해 증가한 염증매개인자인 IL-6, IL-8, $PGE_2$를 유의하게 감소시켰다. 또한, 피부 collagen의 정상적인 구조 및 양에 영향을 미치는 단백질들의 발현을 측정한 결과 HaCaT에서는 UVB 조사로 인해 증가한 TRPV-1과 MMP-1 단백질의 발현이 두릅순 에탄올 추출물의 전처리로 모두 감소하였고, HDF에서는 UVB를 조사한 대조군에 비하여 두릅순 추출물 처리가 MMP-1 단백질 발현을 감소시키는 동시에 collagen의 전구체인 type-I procollagen의 발현을 증가시키는 효과를 보였다. 이들 결과들로부터 항산화효과가 우수한 두릅순 70% 에탄올 추출물은 피부세포에서 UVB에 의한 염증반응을 억제시키는 동시에 피부 collagen의 감소를 억제시킴으로써 피부 광노화를 예방할 수 있는 천연 소재로 이용될 수 있다고 본다.

• Journal of Acupuncture Research
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• 제27권4호
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• pp.1-8
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• 2010

목적 : 본 연구는 녹용약침액의 주름 개선 효과에 대하여 여러 가지 in vitro 실험을 통해 확인해 보고자 계획되었다. 방법 : 녹용약침액의 항산화효과를 DPPH(1,1-diphenyl-2-picrylhydrazyl) 자유라디칼 소거법을 이용하여 측정하였고, 또, elastase 억제 효과를 측정하였다. 그리고 녹용약침액이 사람 정상 섬유아세포 HS 68에서 UVB 조사 후 type I procollagen의 생산량 회복에 미치는 효과를 ELISA법을 이용하여 측정하였다. 결과 : 녹용약침액에서 우수한 DPPH 자유라디칼 소거 효과가 관찰되었다. 또한 통계적으로 유의한 elastase 활성 억제 효과를 관찰할 수 있었다. 사람 섬유아세포 HS 68을 이용한 실험에서는 녹용약침액 처치군에서 UVB 조사로 감소된 type I procollagen이 유의하게 회복되는 것이 관찰되었다. 결론 : 본 연구 결과에 의하면, 녹용약침액에는 유효한 주름개선 효과가 있어, 미용약침 소재로 개발할 수 있을 것으로 사료된다.

• 대한본초학회지
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• 제30권6호
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• pp.1-6
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• 2015

Objectives : Draconis Resina (DR), the resin of Daemonorops draco Bl., is used to circulate the blood and to stop bleeding. It also has been used to generate flesh including ulceration. The present study investigated the effects of DR extract on collagen metabolism in human fibroblasts and tyrosinase activity in mushroom tyrosinase.Methods : The effect of DR extract on type I procollagen production (collagen type I synthesis) and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts cell line. Hs68 cells after ultraviolet B (UVB, 312 nm) irradiation was measured using the enzyme - linked immunosorbent assay (ELISA). The tyrosinase activity was also measured to find out the whitening effects in mushroom tyrosinase by ELISA method.Results : There was no cytotoxicity at DR extract at concentrations of 10 μg/ml, 30 μg/ml, and 100 μg/ml. DR extract significantly inhibited the increase of collagenase activity, whereas it did not show on the reduction of type I procollagen in UVB damaged Hs68 cells. DR extract did not reduce the L - DOPA oxidation. However, it significantly reduced the tyrosinase activity by DR extract at concentraions of 0.1 mg/ml, 1 mg/ml and 10 mg/ml.Conclusions : In conclusion, DR showed the anti-wrinkle and whitening effects via the inhibition of collagenase production and the tyrosinase activity. These results suggest that DR may have potential as an anti-aging ingredient in cosmetic herb markets.