• Journal of Biomedical Engineering Research
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• v.18 no.3
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• pp.233-241
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• 1997

The research on chromosomes is very significant in cytogenetics since genes of the chromosomes control revelation of the inheritance plasma. The human chromosome analysis is widely used to study leukemia, malignancy, radiation hazard, and mutagen dosimetry as well as various congenital anomalies such as Down's, Klinefelter's, Edward's, and Patau's syndrome. The framing and analysis of the chromosome karyogram, which requires specific cytogenetic knowledge is most important in this field. Many researches on automated chromosome karyotype analysis methods have been carried out, some of which produced commercial systems. However, there still remains much room to improve the accuracy of chromosome classification and to reduce the processing time in real clinic environments. In this paper, we proposed a hierarchical artificial neural network(HANN) to classify the chromosome karyotype. We extracted three or four chromosome morphological feature parameters such as centromeric index, relative length ratio, relative area ratio, and chromosome length by preprocessing from ten human chromosome images. The feature parameters of five human chromosome images were used to learn HANN and the rest of them were used to classify the chromosome images. The experiment results show that the chromosome classification error is reduced much more than that of the other researchers using less feature parameters.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.67-71
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• 2001

Isolation and Growth Characteristics of AIkalophilic Bacillus sp. for Removal of Anthraquinone Dye. Kim, Jeong-Mog. School of Environmental Information, Taekyeung College, Kyungsan, 712-850, Korea -Alkalophilic strain degrading and decolorizing anthraquinone dye, Remazol brilliant blue R was isolated from natural system and named as Bacillus sp. ARB!. The optimal temperature and pH of Bacillus sp. ARBI were 35°C and 9.0, respectively. The pH of culture media during the fermentation were changed from 10 and 10.5 of initial values to 9.3 and 9.4 after 40 hrs, respectively. Decolorization efficiency in aerobic shaking culture of Bacillus sp. ARBI was markedly higher than that in standing culture. At the optimal culture condition, decolorization efficiency by the Bacillus sp. ARBl was 93% after 32 hrs batch culture. In the case of batch culture using real dye processing wastewater, dye decolorization efficiency of Bacillus sp. ARBl was 78% after 40 hrs.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1979.04a
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• pp.113.2-114
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• 1979

$\beta-Galactosidase$ is an enzyme which catalizes hydrolysis of lactose, a natural substrate, to glucose and galctose and transferring some monosac-charide units to active acceptors as sugar or alcohol. The occurence of $\beta-Galactosidase$ is known in various microorganisms, animals and higher plants and has been studied by many investigatigators. Especially, a great deal of articles for the enzyme of E. coli have been presented in genetic control mechanism and induction-repression effects of proteins, On the other hand, in the dairly products industry, it is important to hydrolyes lactosd which is the principal sugar of milk and milk products. During the last few years, the interest in enzymatic hydrolysis of milk lactose has teen increased, because of the lactose intolerence in large groups of the population. Microbial $\beta-Galactosidases$ are considered potentially most suitable for processing milk to hydrolyse lactose and, in recent years, the immobilized enzyme from yeast has been examined. Howev, most of the microbial $\beta-Gal$ actosidase are intracellular enzymes, except a few fungal $\beta-Gala-$ ctosidases, and extracellular $\beta-Galactosidase$ which may be favorable to industrial applieation is not so well investigated. On this studies, a mold producing a potent extracellular $\beta-Galactosidase$ was isolated from soil and identified as an imperfect fungus, Beauveria bassians. In this strain, both extracellular and intracellular $\beta-Galactosidases$ were produced simultaneously and a great increase of the extracellular production was acheved by improving the cultural conditions. The extracellular enzyme was purified more than 1, 000 times by procedures including Phosphocellulose and Sephadex G-200 chromatographies. Several characteristics of the enzymewas clarified with this preparation. The enzyme has a main subunit of molecular weight of 80, 000 which makes an active aggregate. And at neutral pH range, it has optimum pH for activity and stability. The Km value was determined to be 0.45$\times$10$^{-3}$ M for $o-Nitrophenyl-\beta-Galactoside.$ In any event, it is interesting to sttudy the $\beta-Galactosidase$ of B. bassiana for the mechanism of secretion and conformational structure of enzyme.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.4
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• pp.251-255
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• 2017

Burr generation is inevitable during the machining of a micro-pattern, and it is difficult to distinguish between the pattern and burr because they have a very small dimensions. In this study, a micro-pattern with a pitch of $60{\mu}m$and height of $1{\mu}m$ was fabricated on a cylindrical surface using a turning machine. The structure of a burr and its generation mechanism were determined, and a magnetic abrasive deburring process was used to improve the accuracy of the pattern. As a result, when fabricating a micro-pattern, it was shown that the direction of the burr was determined by the feed direction of the tool. The measured pattern height was $1.018{\mu}m$ when the magnetic flux density and spindle speed were respectively 40 mT and 1600 rpm, respectively, during magnetic abrasive deburring, which were determined to be the optimal conditions for processing.

• Archives of design research
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• v.15 no.3
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• pp.157-166
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• 2002

This research verified advertising effect according to ad information format and layout based on resource-matching theory. Existing researches suggested inconsistent results that it's effective to design advertising easy to understand by using factual information presentation and integrated layout or it's effective do design difficult advertising to understand by using explanatory information presestation and separate layout. The result of this study suggest that advertising effect by advertising design style is different according to situational elements such as motivation level of information processing and brand hierarchy. The results show that, in the high prestige brand, easily designed advertising using factual information presentation and integrated layout is more effective because consumers make favorable inference by remained cognitive resources. Contrary to this in the low prestige brand, not easily designed advertising using explanatory information and separate layout is more effective because consumers have no remained cognitive resources so that they concentrate on advertising itself.

• Archives of design research
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• v.17 no.4
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• pp.107-118
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• 2004

Nowadays Industrial Design appears to us as provided products in the market through only User Interface and Technical Improvement. Consumer purchasing products intend to grant the meaning for the product by mobilizing his specific feeling and experience the moment he contact products the first time. As metaphorical character shown in the product is named to the Product Language, Industrial designer shall constantly proceed the study on the exact understanding or processing method. As Objects or products using by us imply aesthetical value, it appears to be different according to the diverse condition of products be different according to the diverse condition of products to be used. In case objectivity is unsatisfactory, aesthetic character shown in the product requires new instrumental system. Product analysis applied aesthetical measure of BIRKHOFF can be applied as tool for inherent metaphorical character of product and product development. With this, Design operation shall be much easier. So Application of aesthetical measure is not only a system but a tool giving help to carry out more smooth design process.

• Archives of design research
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• v.17 no.4
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• pp.51-60
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• 2004

The medical instrument industry which is one of the high value added industry has been neglected on the various system and political support because it has been recognized as an unknown field to the public although it is the very easy-approaching field for the export market that minimize a technological gap between advanced country and Korea. Especially, the product design that is very important element for the efficient medical treatment and the psychological stabilization of patient is a typical field of lack of investment and support by the unconcern and lack of understanding of design. Therefore this research suggests the current problem of design with the general inquiry of internal and external market situation and domestic medical instrument industry and also it has the conclusion of an alternative proposal for the solution with the design development processing of a moving type-image diagnostic equipment. We will emboss the importance of design competitiveness, suggest the model and basic data to the medical instrument company that starts later, and be a role of inspiration for the lasting investible desire with this research.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.418-424
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• 2014

High-acidity vinegar was manufactured using pear concentrate by fed-batch fermentation without additional nutrients, and the physicochemical properties and volatile components were investigated at different fermentation stages (Stages 1-4) and at various initial alcohol concentrations (IAC; 6-9%). The levels of reducing sugar, free amino acids, total phenolic content, total flavonoid content, and radical scavenging ability increased slightly during Stage 4 (high-acidity vinegar), which was affected by alcohol feeding. The contents of approximately 20 types of volatile compounds differed between the moderate- and high-acidity vinegar samples, as determined by solid-phase microextraction/gas chromatography-mass spectroscopy. The level of acetic acid in high-acidity vinegar increased according to the initial alcoholic content applied. The high-acidity vinegar produced by fed-batch culture at an IAC of 6-7% showed improved physicochemical and volatile properties as compared to the moderate-acidity vinegar.

• Journal of the Korea Convergence Society
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• v.11 no.9
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• pp.351-358
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• 2020

The purpose of this study is to investigate the effect of gratitude disposition of geriatric hospital employees on job satisfaction and life satisfaction, and the moderating effect of the gratitude diary experience. A total of 485 copies of the research questionnaire were selected for workers in four geriatric hospitals who are promoting campaigns related to the gratitude diary. For data processing, a simple or hierarchical regression analysis was performed using SPSS 26.0. As a result of the study, The gratitude disposition had a statistically significant effect on job satisfaction and life satisfaction. However, the effect of gratitude disposition on job satisfaction and life satisfaction and the moderating effect of the audit diary experience were not statistically significant. The purpose of this study is to develop programs for job satisfaction and life satisfaction of geriatric hospital employees and provide basic data on manpower management of geriatric hospital.

• Journal of Ocean Engineering and Technology
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• v.18 no.6 s.61
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• pp.79-83
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• 2004

The curved blocks which compose the bow and stem of a ship contain many skewed joints that are inclined horizontally and vertically. Most of these joints have a large fitness error and are continuously changing their form and are not easily accessible. The welding position and parameter values should be appropriately set in correspondence to the shape and the inclination of the joints. The welding parameters such as current, voltage, travel speed, and melting rate, are related to each other and their values must be in a specific limited range for the sound welding. These correlations and the ranges are dependent up on the kind and size of wire, shielding gas, joint shape and fitness. To determine these relationships, extensive welding experiments were performed. The experimental data were processed using several information processing technologies. The regression method was used to determine the relationship between current voltage, and deposition rate. When a joint is inclined, the weld bead should be confined to a the limited size, inorder to avoid undercut as well as overlap due to flowing down of molten metal by gravity. The dependency of the limited weld size which is defined as the critical deposited area on various factors such as the horizontally and vertically inclined angle of the joint, skewed angle of the joint, up or down welding direction and weaving was investigated through a number of welding experiments. On the basis of this result, an ANN system was developed to estimate the critical deposited area. The ANN system consists of a 4 layer structure and uses an error back propagation learning algorithm. The estimated values of the ANN were validated using experimental values.