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Changes of Antioxidative Activity and Related Compounds on the Chungkukjang Preparation by Adding Drained Boiling Water (콩 삶은 물을 첨가한 청국장 제조시 항산화활성 및 관련 성분들의 변화)

  • Lee Kyung-Hee;Ryu Seung-Hee;Lee Young-Soon;Kim Young-Man;Moon Gap-Soon
    • Korean journal of food and cookery science
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    • v.21 no.2 s.86
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    • pp.163-170
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    • 2005
  • Soybean is an important plant as the source of protein and oil, as well as phytochemicals such as genistien, daidzein, phenolic acids, phytic acid, tocopherol, and saponin. Chungkukjang, a fermented soybean paste, is common in Korean meals and bacillus is usually used in the fermentation of steamed soybean. For its processing, whole soybeans are boiled in water until the beans are soft, and then the drained beans are wrapped with rice straw or starter and set in a warm place at $65^{\circ}C\;for\;3\;\~\;4\;days$. Normally, the remaining cooked water which was drained from the steamed beans is discarded. We supposed that this water possesses hish amounts of useful components, and we therefore developed a modified method using the cooked water. After fermentation, we added the remaining cooked water which had been drained from the beans to the fermented soy beans and boiled them together. To investigate the bio-functionality of the modified Chungkukjang, the total antioxidative activity, isoflavones contents, phenolic acids, and 3-deoxyglucosone (3-DG) were measured at each stage of the preparation of chungkukjang. The original and modified chungkukjang possessed a high antioxidative activity compared with the other samples, as did the drained water after steaming of the soybean. The contents of genistein, daidzein, and phenolic acids, which contained antioxidative activity, were also increased in the original chungkukjang and their contents were similar in the modified chungkukjang. The content of 3-DG was increased in the modified chungkukjang compare with the original. It is suggested that the active soybean components delivered to the drained water during the steaming process were useful for increasing the bio-functionality of the modified chungkukjang.

Evaluation of Biological Critical Control Points Using Escherichia coli Genotyping (Escherichia coli Genotype을 이용한 생물학적 Critical Control Point의 적합성 평가)

  • Kim, Hak-Jae;Hahn, Tae-Wook;Juong, Ji-Hun;Bahk, Gyung-Jin;Hong, Chong-Hae
    • Food Science of Animal Resources
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    • v.29 no.6
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    • pp.695-701
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    • 2009
  • This study was performed to evaluate the effectiveness of biological critical control points using the genetic profile of Escherichia coli isolates from pork cutting plants. Samples were collected from carcasses, equipment (knife, table, glove, transport belt, boning and skinning machine), the environment (wall and floor), and meat cuts during the cutting process from two plants. Pulsed-field gel electrophoresis (PFGE) was used to characterize the E. coli isolates. An identical genotype was detected from the carcasses, equipment, environment, and final meat cuts, and showed that the incoming carcasses, which were contaminated during transportation from slaughterhouses, were a major source of E. coli that was spread throughout processing. Also, consistent cross-contamination due to improper cleaning and disinfection procedures was another possibility. As a result, incoming carcasses and cleaning procedures should be considered critical control points in pork cutting plants, since a heating step is not used to inactivate microorganisms. Furthermore, the high rate (59.6%) of E. coli isolation indicates E. coli can be a good indicator in livestock processing plants even though it has genetic diversity.

Changes in Physical Properties and Its Metal Removal Efficiency for The Yellow Soils by Calcination Process (소성처리에 의한 황토의 물성특성 변화 및 용존 중금속 제거능력)

  • Lee, Jin-Won;Kim, Seokhwi;Hwang, Gab-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.584-591
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    • 2017
  • Metal removal from water has not been explained clearly by either adsorption onto the surface of absorbents or precipitation as metal hydroxides because those occur simultaneously to a certain extent. For a better understanding of the metal removal mechanisms, batch experiments were performed using soil calcined at $850^{\circ}C$ under various pH conditions for Cu, Pb, Zn, Cd, and Cr. The results showed that the metal removal efficiency with the exception of Cr decreased abruptly, even within 5 min, showing more than 90% removal. The pH of each reactant increased gradually from around 7 to 9 with time. The increases in metal removal at higher pH appear to be associated with metal hydroxides precipitation. Comparative experiments, which were carried out changing the pH by reacting with commercial activated carbon (CAC), natural yellow soil (NYS), and calcined yellow soil (CYS), showed that the pH of the CYS only increased with time. Calcination processes might lead to a change in the physical properties of the soil matrix resulting in a high pH when reacted with water. Apart from adsorption onto the surface of the absorbents, these results show that the adsorption and/or precipitation of hydroxides onto the surface of adsorbents also play important roles in regulating the dissolved metals under alkaline conditions.

Change of Vegetation Structure for 6 years (1994-1999) at the Harvested Forest Area (I) - A Case of (Mt.) Baegwoonsan Research Forest at Kwangyang City - (산림(山林) 벌채적지(伐採跡地)의 6년간 식생구조(植生構造) 변화(變化) (I) - 광양시(光陽市) 백운산(白雲山) 연습임지역(演習林地域)을 중심(中心)으로 -)

  • Jee, Yong-Ki;Oh, Koo-Kyoon
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.673-682
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    • 2001
  • The objective of this study was to monitor vegetation development process after timber harvesting at (Mt.) Baekwoonsan Seoul National University Forests, Korea. Two monitoring plots were established in 1994 and woody plant were monitored from 1994 to 1999. Vegetation development pattern during last six years(1994-1999) after timber harvesting were as follows; Styrax obassia, Styrax japonica and Lindera erythrocarpa as of the existing tree were competitive species in the first year after clearcut, and Styrax japonica and Lindera erythrocarpa as of sprout tree) and Aralia elata as of seedling were dominant species in the sixth years after clearcut. Species diversity indices of harvested forest interior was decreased at the southwestern slope and increased at the northeastern slope. According to DBH distribution pattern, No. of individuals of Lindera erythrocarpa and Aralia elata showed vigorous growth in the sub-canopy layer and then Styrax japonica, Quercus serrata, Maackia amurensis, Lespedeza maximowixzii, Lindera obtusiloba, Staphylea bumalda, Clero-dendrum trichotomum, Weigela subsessilis, in order showed good growth in the sixth year after clearcut. Lindera erythrocarpa with a reversed J-shaped curve pattern by DBH class will be increased while Aralia elata with a J-shaped curve pattern will be decreased.

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Effects of Cutting Condition on Rooting and Early Growth of a Rare Endemic Juniperus chinensis var. sargentii HENRY (삽목조건이 희귀수종 눈향나무의 발근과 초기생장에 미치는 영향)

  • Ahn, Sang-Yeol;An, Byung-Seop;Park, Soo-Jeong;Kim, Zhoo-Hyeon;Lee, Sang-Woo
    • Journal of agriculture & life science
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    • v.45 no.4
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    • pp.73-80
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    • 2011
  • This study was conducted to elucidate the best cutting condition in propagation of Juniperus chinensis var. sargentii in respect of survival rate, rooting rate, and early growth after cutting via process of cutting time, cutting media, cutting type and growth regulators. As for cutting time, rooting rate was 68.3% on April, which was higher than 39.5% on May and 32.5% on June. The best cutting media was stream sand of rooting rate 72.7%. Those of mixed soil and decomposition were 40.1% and 27.5% each. The effect of growth regulators was different according to the kind of cutting media. There was no effect on stream sand media which showed the highest rooting rate, while it was very effective on the media of decomposition of granite, which rooting rate showed the lowest. Hardwood as cutting materials showed somewhat higher survival and rooting rate compared to semi-hardwood. The cutting with hardwood condition to stream sand on April, it is regarded to be the most effective condition for Juniperus chinensis var. sargentii to increase survival and rooting rate. Therefore in this experiment it is considered that the best season cutting is April, and the most useful media and plant materials stream sand, and hardwood respectively.

Smart Synthetic Path Search System for Prevention of Hazardous Chemical Accidents and Analysis of Reaction Risk (반응 위험성분석 및 사고방지를 위한 스마트 합성경로 탐색시스템)

  • Jeong, Joonsoo;Kim, Chang Won;Kwak, Dongho;Shin, Dongil
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.781-789
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    • 2019
  • There are frequent accidents by chemicals during laboratory experiments and pilot plant and reactor operations. It is necessary to find and comprehend relevant information to prevent accidents before starting synthesis experiments. In the process design stage, reaction information is also necessary to prevent runaway reactions. Although there are various sources available for synthesis information, including the Internet, it takes long time to search and is difficult to choose the right path because the substances used in each synthesis method are different. In order to solve these problems, we propose an intelligent synthetic path search system to help researchers shorten the search time for synthetic paths and identify hazardous intermediates that may exist on paths. The system proposed in this study automatically updates the database by collecting information existing on the Internet through Web scraping and crawling using Selenium, a Python package. Based on the depth-first search, the path search performs searches based on the target substance, distinguishes hazardous chemical grades and yields, etc., and suggests all synthetic paths within a defined limit of path steps. For the benefit of each research institution, researchers can register their private data and expand the database according to the format type. The system is being released as open source for free use. The system is expected to find a safer way and help prevent accidents by supporting researchers referring to the suggested paths.

Effect of Ammonium Nitrate Plus Potash in Comparison with Urea Plus Potash on the Yield and Content of Some Mineral Nutrient Elements of Chinese Cabbage (요소+칼리에 대비(對備)한 질산암모늄+칼리가 배추의 수량(數量) 및 무기성분함량(無機成分含量)에 미치는 영향(影響))

  • Oh, Wang-Keun;Kim, Sung-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.407-412
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    • 1985
  • The effect of potash applied with different sources of nitrogen was experimented in pot soil culture on chinese cabbage (Brassica Pekinensis Rupr, Var. Fall 1984: Sammi Garak, Spring 1985: Jungang Summer lab.) in the fall, 1984 and in the spring, 1985. Results obtained are as follows; 1. Ammonium nitrate increased the yield of chinese cabbage more than urea did, and the effect of yield increase by ammonium nitrate was greater in the fall cultivation than in the spring. 2. The yield of chinese cabbage was positively correlated with the contents of K in the first (May 17, 1985) and second (June 9, 1985) thined cabbages (dry matter). It was also positively correlated with $NO_3-N$ content of the first thined cabbage and with K/Ca+Mg m.e, ratio of outer leaves of the harvested (June 27, 1985) cabbage, but negatively affected with Mg content of the outer leaves. 3. Correlations between K and $NO_3-N$ contained in the dry matter of first and second thined, and inner leaves of the harvested cabbage were learnt to be $r;0.9998^{**}$, r;0.4439, and $r;-0.7135^*$ respectively. The higher $NO_3-N$ content in the inner leaves of harvested cabbage was observed at K omitted ammonium nitrate plot where K was deficient, Ca and Mg contents were low. 4. The nutrient absorption and growth of chinese cabbage may take the following process. Nitrate nitrogen increases vegetative growth of the plant with enhanced K uptake and movement in to inner leaves and followed by replacement of Ca uptake and finally Mg uptake and its movement in to inner leaves.

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Effect of Soil Salinity and Flooding on Plant Growth and Yield of Rape-Castor Bean Cropping System in the Newly Reclaimed Tidal Land of Western Seaside of Korea (서해안 신간척지에서 유채-피마자 작부체계시 토양염농도 및 침수가 생육 및 수량에 미치는 영향)

  • Sohn, Yong-Man;Jeon, Geon-Yeong;Song, Jae-Do;Lee, Jae-Hwang;Park, Moo-Eon
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.355-363
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    • 2009
  • Double cropping system of oilseed crops introduced rape and castor bean was studied in the newly reclaimed tidal land of Korea. Surface soil EC to reach at 50% of growth reduction to the tallest height of crops was estimated $4dS\;m^{-1}$ for castor bean and $6dS\;m^{-1}$ for rape by logarithmic function. The castor bean cultivated during rainy summer much more suffered serious growth and yield reduction by flooding damage in the Hwaong and Yeonsangang reclaimed lands having low soil conductivity and finer textured soil than in the Iweon reclaimed land having higher soil conductivity and coarse sandy textured soil. Rape cultivated during dry winter-spring much more suffered serious growth and yield reduction by high soil salinity come from re-salting process. 50% yield reduction was estimated at $2.0\;2.5dS\;m^{-1}$ of surface soil EC by logarithmic function. Consequentially, it was concluded that oilseed production for energy by double cropping system of rape and castor bean might be possible under good controling soil salinity below $4dS\;m^{-1}$ for castor and $3dS\;m^{-1}$ for rape in the newly reclaimed land of Korea.

Anti-obesity and Anti-diabetes Effects of the Fermented White Jelly Fungus (Tremella fuciformis Berk) Using Lactobacillus rhamnosus BHN-LAB 76 (유산균을 이용한 흰목이 버섯의 발효를 통한 항비만과 항당뇨 효과)

  • Lee, Jun-Hyeong;Kim, Byung-Hyuk;Yoon, Yeo-Cho;Kim, Jung-Gyu;Park, Ye-Eun;Park, Hye-Suk;Hwang, Hak-Soo;Kwun, In-Sook;Kwon, Gi-Seok;Lee, Jung-Bok
    • Journal of Life Science
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    • v.29 no.4
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    • pp.470-477
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    • 2019
  • White jelly fungus (Tremella fuciformis Berk) is effective for hypertension, diabetes, obesity, lung disease, beauty, and has been known as an elixir of life in ancient Chinese dynasty. In this study, we investigated the anti-obesity and diabetic effects of the fermented white jelly fungus extract by the bioconversion process. We fed an obesity-inducing mouse with 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremella fuciformis (FTF), and 5% FTF containing High Fats Diet (HFD) and HFD for 8 weeks, respectively. The oral glucose tolerance test (OGTT) was performed analysis after 7 weeks of feeding and the dietary intake, food efficiency ratio, body weight, liver, epididymal fat weight, and serum insulin level were measured after 8 weeks of feeding. Also, HOMA-IR was analyzed. The concentrations of serum total cholesterol, triglycerides test was analyzed. The FTF compare with 5% TF and HFD confirmed that 5% FTF reduced body weight, tissue weight, triglycerides concentration, HOMA-IR, respectively. As a result, we confirmed that the fermented white jelly fungus has the anti-obesity effect. Finally, this study can be used a basic data for obesity treatment using fermented white jelly fungus.

Inhalation of Bacterial Cellulose Nanofibrils Triggers an Inflammatory Response and Changes Lung Tissue Morphology of Mice

  • Silva-Carvalho, Ricardo;Silva, Joao P.;Ferreirinha, Pedro;Leitao, Alexandre F.;Andrade, Fabia K.;da Costa, Rui M. Gil;Cristelo, Cecilia;Rosa, Morsyleide F.;Vilanova, Manuel;Gama, F. Miguel
    • Toxicological Research
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    • v.35 no.1
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    • pp.45-63
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    • 2019
  • In view of the growing industrial use of Bacterial cellulose (BC), and taking into account that it might become airborne and be inhaled after industrial processing, assessing its potential pulmonary toxic effects assumes high relevance. In this work, the murine model was used to assess the effects of exposure to respirable BC nanofibrils (nBC), obtained by disintegration of BC produced by Komagataeibacter hansenii. Murine bone marrow-derived macrophages ($BMM{\Phi}$) were treated with different doses of nBC (0.02 and 0.2 mg/mL, respectively 1 and $10{\mu}g$ of fibrils) in absence or presence of 0.2% Carboxymethyl Cellulose (nBCMC). Furthermore, mice were instilled intratracheally with nBC or nBCMC at different concentrations and at different time-points and analyzed up to 6 months after treatments. Microcrystaline $Avicel-plus^{(R)}$ CM 2159, a plant-derived cellulose, was used for comparison. Markers of cellular damage (lactate dehydrogenase release and total protein) and oxidative stress (hydrogen peroxidase, reduced glutathione, lipid peroxidation and glutathione peroxidase activity) as well presence of inflammatory cells were evaluated in brochoalveolar lavage (BAL) fluids. Histological analysis of lungs, heart and liver tissues was also performed. BAL analysis showed that exposure to nBCMC or CMC did not induce major alterations in the assessed markers of cell damage, oxidative stress or inflammatory cell numbers in BAL fluid over time, even following cumulative treatments. $Avicel-plus^{(R)}$ CM 2159 significantly increased LDH release, detected 3 months after 4 weekly administrations. However, histological results revealed a chronic inflammatory response and tissue alterations, being hypertrophy of pulmonary arteries (observed 3 months after nBCMC treatment) of particular concern. These histological alterations remained after 6 months in animals treated with nBC, possibly due to foreign body reaction and the organism's inability to remove the fibers. Overall, despite being a safe and biocompatible biomaterial, BC-derived nanofibrils inhalation may lead to lung pathology and pose significant health risks.