• KSBB Journal
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• 제21권5호
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• pp.341-346
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• 2006

본 연구에서는 sonificator를 장착하여 세포막을 파쇄하고 현장에서 형광을 이용하여 조작이 간편하고 단시간에 DNA를 측정할 수 있는 자동화된 형광기를 개발하기 위하여 그람 음성균인 Escherchia coli를 대상으로 최적의 세포 파쇄조건을 확립하고자 하였다. Incubation time은 형광량에 크게 영향을 주지 못하는 것으로 나타났으며, 가열처리 방법은 현장에서 세포를 파괴하는 방법으로는 파쇄효과가 미약하고 적합하지 않은 것으로 나타났다. Sonificator probe 직경에 따라 세포의 파쇄 효과가 큰 차이를 보였으며 13 mm probe로 20초 동안 sonification시키는 것이 가장 효율적인 것으로 나타났다. 시료에 잠긴 Sonificator probe tip 깊이에 따라서도 세포의 파쇄 효과가 크게 나타났는데 시료에 잠긴 probe tip의 깊이가 깊을수록 큰 파쇄 효과를 발휘하였다. 선정된 최적의 파쇄 조건에서 $5{\times}10^5CFU/m{\ell}$의 Escherchia coli를 탐지 가능한 것으로 나타났다.

• International Journal of Safety
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• 제7권2호
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• pp.1-6
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• 2008

In this paper, the effects of the position and the angle of the potential probes on the measurements of the ground resistance using the fall-of-potential method are described and the testing techniques for minimizing the measuring errors are proposed. The fall-of-potential method is theoretically based on the potential and current measuring principle and the measuring error is primarily caused by the position and angle of auxiliary probes. In order to analyze the relative error in the measured value of the ground resistance due to the position of the potential probe, the ground resistance was measured for the case in which the distance of the current probe was fixed at 50[m] and the distance of the potential probe was located from 10[m] to 50[m]. Also, the potential probe was located in turn at $30[^{\circ}]$, $45[^{\circ}]$, $60[^{\circ}]$, $90[^{\circ}]$, and $180[^{\circ}]$. As a consequence, relative error decreased with increasing distance of the potential probe and decreasing angle between the current probe and potential probe. The results could help to determine the position of the potential probe during the ground resistance measurement.

• Physical Therapy Rehabilitation Science
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• 제8권4호
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• pp.194-201
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• 2019

Objective: This study aimed to investigate the reliability and validity of muscle thickness (MT) and pennation angle (PA) measurements of the ankle muscle, including the tibialis anterior (TA) and the medial gastrocnemius (MGCM), using a hands-free fixed probe and to compare it with the conventional linear probe. Design: Observational inter-rater reliability study. Methods: Thirty-three healthy subjects (20 male, 13 female) were included. In all subjects, ultrasound images were acquired from the TA and MGCM using a hands-free fixed probe and a conventional linear probe in random sequence by two examiners at two time-points within a 7-day interval. MT and PA were calculated on the taken images. Intra-class correlation coefficients (ICC), 95% confidence intervals, standard error of measurement and the Pearson's correlation coefficient were used to estimate reliability and validity. And also, Bland-Altman plots were generated for a visual representation of MT and PA at the TA and MGCM. Results: The ICC for all intra-rater reliability was 0.943 to 0.995 and that for all inter-rater reliability was 0.928 to 0.993, indicating excellent reliability. A significantly high correlation was observed between MT and PA at the TA and MGCM with use of the hands-free fixed probe and the conventional linear probe (r>0.938; p<0.001). Conclusions: The hands-free fixed probe provided excellent images for measurement of the MT and PA of the TA and MGCM and is a useful device for making clinical measurements of muscle structure without grasping of the probe.

• 센서학회지
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• 제25권5호
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• pp.344-348
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• 2016

We developed a 2-channel fiber-optic temperature sensor (FOTS) using a temperature sensing probe, a fiber-optic coupler, transmitting optical fiber, and an optical time domain reflectometer (OTDR). The temperature sensing probe is divided into a sensing probe and a reference probe for accurate thermometry. A sensing probe is composed of a silicon oil, a FC terminator, a brass pipe, and a singlemode optical fiber and the structure of a reference probe is identical with that of the sensing probe excluding a silicon oil. In this study, we measured the modified optical powers of the light signals reflected from the temperature sensing probe placed inside of the water with a thermal variation from 5 to $70^{\circ}C$. Although the optical power of the reference probe was constant regardless of the temperature change, the optical power of the sensing probe decreased linearly as the temperature increased. As experimental results, the FOTS using a subtraction method showed a small difference (i.e., hysteresis) in its response due to heating and cooling. The reversibility and reproducibility of the FOTS were also evaluated.

• 조명전기설비학회논문지
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• 제23권2호
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• pp.96-102
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• 2009

본 논문에서는 전위강하법을 이용한 접지저항 측정시 전위보조전극의 위치 및 각도의 영향에 대하여 기술하였으며, 측정시 오차를 최소화하는 기법을 제안하였다. 전위강하법은 이론적으로 전위와 전류의 측정원리에 근간을 두고 있으며 측정오차는 주로 보조전극의 위치와 각도에 기인한다. 전위보조전각의 위치에 의한 접지저항 측정값의 상대오차를 분석하기 위해 전류보조전극의 거리를 50[m]로 고정시키고 전위보조전극의 거리를 10[m]에서 50[m]까지 변화시키며 접지저항을 측정하였고 또한 전위보조전극과 전류보조전극 사이의 각도를 30[$^{\circ}$], 45[$^{\circ}$], 60[$^{\circ}$], 90[$^{\circ}$], 180[$^{\circ}$]로 변화시키며 측정하였다. 결과적으로 전위보조전극의 거리 증가 및 전류보조전극과 전위보조전극 사이의 각도가 감소할수록 상대오차가 작게 나타났다. 본 실험결과는 접지시스템의 접지저항을 측정할 때 전위보조전극의 위치를 결정하는데 활용될 수 있다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.140.1-140.1
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• 2016

We have measured DNA translocation through a nanocapillary functionalized with probe DNA. These DNA-functionalized nanocapillaries selectively facilitate the translocation of target ssDNAs that are complementary to the probe ssDNAs. In addition, translocation of the complementary target ssDNA exhibits two tendencies to translocation speed, such as fast and slow translocation, whereas that of non-complementary target ssDNA yields only one tendency, fast translocation. These observations suggest that the complementary and non-complementary target ssDNAs may be discriminated due to different interaction strengths between target and probe ssDNAs. The temperature dependence measurements of DNA translocation show that slow translocation events are ascribed to the complementary interaction between probe and target ssDNA. This confirms that their dwell time is dependent on the base-pair binding strength. These results demonstrate that mere single-base different target DNA can be selectively detectable by using the probe DNA-functionalized nanocapillaries.

• Journal of Advanced Marine Engineering and Technology
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• 제27권5호
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• pp.624-632
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• 2003

In the gasoline engine industry. there has been a trend towards the development of high performance engines with improved fuel efficiency, reduced weight and smaller sizes. These trends help to solved engine problems related to thermal load and abnormal combustion. In order to investigate these Problems, a thin film-type probe for instantaneously measuring temperatures has been suggested. A method for manufacturing such a probe was established in this study. The instantaneous surface temperature of a constant volume combustion chamber was measured by this probe and the heat flux was obtained through Fourier analysis. A peak instantaneous temperature was obtained after 55∼60 ms from ignition and the temperature increased according to an equivalence ratio and varied differently according to the position of the probe. Total heat loss during combustion period was affected by the equivalence ratio and differed widely in accordance to the position of the probe.

• 한국산업융합학회 논문집
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• 제6권3호
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• pp.193-200
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• 2003

In the production of internal combustion engines, there has been a move towards the development of high performance engines with improved fuel efficiency, lighter weight and smaller sizes. These trends help to answer problems in engines related to thermal load and abnormal combustion. In order to investigate these problems, a thin film-type probe for instantaneously measuring temperatures has been suggested. A method for manufacturing such a probe was established in this study. The instantaneous surface temperature of a constant volume combustion chamber was measured by this probe and the heat flux was obtained through Fourier analysis. In order to thoroughly understand the characteristics of combustion, the authors measured the wall temperature of the combustion chamber and computed heat flux through a cylinder wall while varying the protrusion height of the probe. For achieving the above goals, a instantaneous temperature probe was developed, thereby making possible the analysis of the instantaneous temperature of wall surface and the detection of unsteady heat flux in the constant volume combustion chamber.

• 한국잠사곤충학회지
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• 제37권1호
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• pp.16-26
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• 1995

RFLP법에 의한 누에품종의 계통분류를 목적으로 유용 probe의 선발 실험을 행하여, 얻어진 2개의 probe SP1-13, SP1-28과 외부에서 분양 받은 probe10-42를 사용하여 22개 현 장려 누에품종과 멧누에 대한 DNA 다형성을 조사하였다. 조사결과 이들 probe들 중 SP1-28에서 품종간의 다형성을 나타내는 수종의 band가 검출되었고, SP1-13 에서도 다형성을 보이는 소수의 band가 인정되었으나, 10-42으로부터는 모든 품종에서 monomorphic한 한개의 band만이 검출되었다.

• Journal of Electrical Engineering and Technology
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• 제6권1호
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• pp.119-122
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• 2011

A new high speed AFM probe has been proposed and fabricated. The probe is integrated with PZT actuated cantilever realized in bulk silicon wafer using heavily boron doped silicon as an etch stop layer. The cantilever thickness can be accurately controlled by the boron diffusion process. Thick SCS cantilever and integrated PZT actuator make it possible to be operated at high speed for fast imaging. The resonant frequency of the fabricated probe is 92.9 kHz and the maximum deflection is 5.3 ${\mu}m$ at 3 V. The fabricated probe successfully measured the surface of standard sample in an AFM system at the scan speed of 600${\mu}m$/sec.