• 제목/요약/키워드: probe

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사람 Y 염색체 특이 DNA Probe의 개발과 이를 이용한 FISH 기술의 임상적 적용 (Identification of a Human Y Chromosome Specific DNA Probe and Their Clinical Application by Fluorescence in situ Hybridization Techniques)

  • 류란숙;이상찬;이채식;김종흥;류은경;손시환
    • Clinical and Experimental Reproductive Medicine
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    • 제27권4호
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    • pp.405-415
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    • 2000
  • Objective: The present study was undertaken to synthesize a human Y chromosome specific probe and to confirm the usefulness of the probe for fluorescence in situ hybridization (FISH) in various types of human cells. Methods: An approximately 400 bp DNA fragment of the DYZ1 sequences was synthesized by PCR using digoxigenin labeled dUTP (dig-PCR). The fidelity of probe was tested by FISH for cultured and uncultured human lymphocytes, amniocytes, chorionic villus cells, embryos, sperms, and germ cells of seminiferous tubule. Results: The human Y chromosome specific probe hybridized specifically to Y chromosome of the cells that had been tested. This probe assigned to the Yq12 region where the DYZ1 repetitive sequence is concentrated. Conclusion: We have identified a human Y chromosome specific probe that hybridized specifically to the Y chromosome by FISH for various types of uncultured as well as cultured cells. Therefore FISH technique using human Y chromosome specific probe should be useful for clinical application as a diagnostic tool for the detection of human Y chromosome.

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Flexible ECA Probe를 이용한 평판 및 용접부 검사 (Inspection of Welded Zone and Flat Plate Using Flexible ECA Probe)

  • 이창준;이규성;신충호;이경준;장윤영
    • 비파괴검사학회지
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    • 제36권4호
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    • pp.288-294
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    • 2016
  • Flexible ECT (eddy current array) probe를 사용하여 판재 및 용접부에 존재하는 notch 결함을 MS-5800E와 OmniScan MX 장비로 검출능력을 비교하고, 주파수와 lift-off를 변수로 신호의 특성을 비교하고자 하였다. 실험 결과, 500, 1000, 1500 kHz를 사용하였을 때 notch 깊이가 증가할수록 신호의 진폭이 증가하는 것으로 나타났고, lift-off 변화에 따른 신호의 진폭은 선형적으로 감소하였다. 또한 용접부 결함은 probe와 시험체의 접촉면에 밀접한 관계가 있다. Probe와 시험체의 접촉면이 양호한 경우 검출감도가 우수하고 그렇지 않은 경우에는 검출감도가 떨어지는 것을 알 수 있었다.

결측 택시 Probe 통행속도 예측기법 개발에 관한 연구 (A Study on the Development of a Technique to Predict Missing Travel Speed Collected by Taxi Probe)

  • 윤병조
    • 대한토목학회논문집
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    • 제31권1D호
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    • pp.43-50
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    • 2011
  • 택시 프로브(Probe)를 이용한 구간통행속도 모니터링체계는 지능형교통체계(ITS)의 핵심적인 하부시스템 중 하나이다. 택시 프로브기법을 통해 수집되는 구간통행속도는 도시가로망의 교통상태 모니터링과 통행시간 정보제공에 널리 활용되고 있다. 그러나 택시 Probe기법은 표본수가 적고 교통혼잡으로 인하여 구간통행시간이 자료수집 주기보다 큰 경우, 실시간으로 자료가 수집되지 않는 누락상태가 발생하게 된다. 이러한 누락상태는 단일시간대에서 다중시간대에 걸쳐 발생하게 되며, 기존의 단일시간대 예측기법으로는 다중시간대의 상태를 예측하지 못하는 단점이 있다. 따라서 다중시간대 누락상태에서 실시간 구간통행속도를 예측하기위한 기법이 요구된다. 본 연구에서는 기존의 단일시간대 예측기법의 한계를 극복하면서 단일 및 다중시간대 통행속도를 예측하기위한 기법을 개발하였다. 개발된 모형은 비모수회귀(NPR)을 기반으로 개발되었으며, 다중시간대 예측에도 불구하고 기존의 단일시간대 예측기법보다 우수한 정확도를 보였다.

랑뮤어 탐침에 의해 변형된 열플라즈마 특성에 관한 해석적 연구 (Numerical Study on the Characteristics of Thermal Plasmas Disturbed by Inserting a Langmuir Probe)

  • 이종철;김윤제
    • 한국진공학회지
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    • 제17권3호
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    • pp.189-194
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    • 2008
  • 플라즈마 진단법 중 손쉽고 구동이 용이한 장점으로 인해 범용 플라즈마 진단 장치로 사용되는 랑뮤어 (Langmuir) 탐침법은 탐침이 직접 플라즈마 내에 삽입됨으로써 플라즈마를 간섭하기 때문에 플라즈마 고유의 특성을 변형시키는 약점이 있다. 본 연구에서는 대기압 아르곤 자유 연소 아크 (free-burning arc)에 삽입된 랑뮤어 탐침에 의한 교란 영향을 고찰하기 위하여 탐침 유무에 따른 열플라즈마 상태를 수치계산 하였고, 온도장과 속도장이 왜곡된 정도를 비교 분석하였다. 열플라즈마를 모델링하기 위하여 열유동장과 전자기장에 관한 두 종류의 지배방정식을 상용 CFD 프로그램과 자체 제작한 서브루틴을 이용하여 연계 계산하였다. 삽입된 탐침에 의해 열플라즈마 온도장은 탐침의 앞뒤에서 모두 플라즈마 축의 수직 방향으로 큰 변화를 나타내는 열적 교란이 발생되었다. 속도장에서는 탐침 선단의 정체 영역과 후단 후류 영역에서 유동 교란이 발생되었으며, 삽입 된 탐침의 영향이 국부적인 것이 아닌 플라즈마 유동장 대부분 영역에 영향을 미침을 확인할 수 있었다. 따라서 탐침법의 신뢰성을 높이기 위해서는 탐침 삽입에 의한 플라즈마의 열유체역학적 교란을 고려해야 한다.

Profile Measurements of Micro-aspheric Surfaces Using an Air-bearing Stylus with a Microprobe

  • Shibuya, Atsushi;Gao, Wei;Yoshikawa, Yasuo;Ju, Bing-Feng;Kiyono, Satoshi
    • International Journal of Precision Engineering and Manufacturing
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    • 제8권2호
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    • pp.26-31
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    • 2007
  • A novel scanning probe measurement system was developed to enable precise profile measurements of microaspheric surfaces. An air-bearing stylus with a microprobe was used to perform the surface profile scanning. The new system worked in a contact mode and had the capability of measuring micro-aspheric surfaces with large tilt angles and complex profiles. Due to limitations resulting from the contact mode, such as possible damage caused by the contact force and lateral resolution restrictions from the curvature of the probe tip, several system improvements were implemented. An air bearing was used to suspend the shaft of the probe to reduce the contact force, enabling fine adjustments of the contact force by changing the air pressure. The movement of the shaft was measured by a linear encoder with a scale attached to the actual shaft to avoid Abbe errors. A $50-{\mu}m-diameter$ glass sphere was bonded to the tip of the probe to improve the lateral resolution of the system. The maximum contact force of the probe was 10 mN. The shaft was capable of holding the probe continuously if the contact force was less than 40 mN, and the resolution of the probe could be as high as 10 nm, The performance of the new scanning probe measurement system was verified by experimental data.

Review paper: Application of the Pulsed Eddy Current Technique to Inspect Pipelines of Nuclear Plants

  • Park, D.G.;Angani, C.S.;Kishore, M.B.;Vertesy, G.;Lee, D.H.
    • Journal of Magnetics
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    • 제18권3호
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    • pp.342-347
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    • 2013
  • Local wall thinning in pipelines affects the structural integrity of industries, such as nuclear power plants (NPPs). In the present study, a development of pulsed eddy current (PEC) technology that detects the wall thinning of pipelines covered with insulation is reviewed. The methods and experimental results, which have two kinds of probe with a single and double core, were compared. For this purpose, the single and double core probes having one and two excitation coils have been devised, and the differential probe with two Hall sensors has been fabricated to measure the wall thinning in insulated pipelines. The test sample is a stainless steel having different thickness, laminated by plastic insulation to simulate the pipelines in NPPs. The excitation coils in the probe is driven by a rectangular current pulse, the difference of two Hall sensors has been measured as a resultant PEC signal. The peak value of the detected signal is used to describe the wall thinning. The double core probe has better performance to detect the wall thinning covered with insulation; the single core probe can detect the wall thinning up to an insulation thickness of 18 mm, whereas the double probe can detect up to 25 mm. The results show that the double core PEC probe has the potential to detect the wall thinning in an insulated pipeline of the NPPs.

Design, analyses, and evaluation of a spiral TDR sensor with high spatial resolution

  • Gao, Quan;Wu, Guangxi;Yu, Xiong
    • Smart Structures and Systems
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    • 제16권4호
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    • pp.683-699
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    • 2015
  • Time Domain Reflectometry (TDR) has been extensively applied for various laboratory and field studies. Numerous different TDR probes are currently available for measuring soil moisture content and detecting interfaces (i.e., due to landslides or structural failure). This paper describes the development of an innovative spiral-shaped TDR probe that features much higher sensitivity and resolution in detecting interfaces than existing ones. Finite element method (FEM) simulations were conducted to assist the optimization of sensor design. The influence of factors such as wire interval spacing and wire diameter on the sensitivity of the spiral TDR probe were analyzed. A spiral TDR probe was fabricated based on the results of computer-assisted design. A laboratory experimental program was implemented to evaluate its performance. The results show that the spiral TDR sensor featured excellent performance in accurately detecting thin water level variations with high resolution, to the thickness as small as 0.06 cm. Compared with conventional straight TDR probe, the spiral TDR probe has 8 times the resolution in detecting the water level changes. It also achieved 3 times the sensitivity of straight TDR probe.

Cutoff Probe를 이용한 자화유도결합 플라즈마의 특성 연구 (A Study on Magnetized Inductively Coupled Plasma Using Cutoff Probe)

  • 손의정;김동현;이호준
    • 전기학회논문지
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    • 제65권10호
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    • pp.1706-1711
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    • 2016
  • Electromagnetic wave simulation was performed to predict characteristics of manufactured cutoff probe at low temperature magnetized plasma medium. Microwave cutoff probe is designed for research the properties of magnetized inductively coupled plasma. It was shown that the cutoff probe method can safely be used for weakly magnetized high density plasma sources. Cutoff probe system with two port network analyzer has been prepared and applied to measure electron density distributions in large area, 13.56MHz driven weakly magnetized inductively coupled plasma source. The results shown that, the plasma frequency confirmed cut-off characteristics in low temperature plasma. Especially, cut-off characteristics was found at upper hybrid resonance frequency in the environment of the magnetic field. In case of a induced weak magnetic field in inductively coupled plasma, plasma density estimated from the cutoff frequency in the same way at unmagnetized plasma due to nearly same plasma frequency and upper hybrid resonance frequency. The plasma density is increased and uniformity is improved by applying a induced weak magnetic field in inductively coupled plasma.