• BMB Reports
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• v.50 no.9
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• pp.435-436
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• 2017

Primed human pluripotent stem cells (hPSCs) are highly dependent on glycolysis rather than oxidative phosphorylation, which is similar to the metabolic switch that occurs in cancer cells. However, the molecular mechanisms that underlie this metabolic reprogramming in hPSCs and its relevance to pluripotency remain unclear. Cha et al. (2017) recently revealed that downregulation of SIRT2 by miR-200c enhances acetylation of glycolytic enzymes and glycolysis, which in turn facilitates cellular reprogramming, suggesting that SIRT2 is a key enzyme linking the metabolic switch and pluripotency in hPSCs.

• Annual Conference on Human and Language Technology
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• 2005.10a
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• pp.57-62
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• 2005

한국어 명사 어절의 시간 경과에 따른 처리 양상을 확인해 보기 위하여 점화어절판단과제(primed eojeol decision task)를 이용한 실험을 실시하였다. 점화어 제시 방식은 차폐점화, SOA 100ms, SOA 300ms 조건으로 하였으며, 점화어-표적어의 관계는 어절일치, 어간일치, 통제조건으로 조작하였다. 그 결과 어절일치조건과 어간일치조건에서 촉진효과가 있었으며, SOA가 증가함에 따라 어절일치조건과 어간일치조건에서의 촉진효과도 함께 증가하는 양상을 확인하였다. 점화어의 제시 방식과 점화어-표적어의 관계간의 상호작용도 유의미하였다. 이러한 결과를 바탕으로 심성어휘집에서의 어절 표상의 실재성과 어절 속성과 어간 속성이 어절 처리 과정에 관여하는 방식에 관하여 논의하였다.

• Korean Journal of Animal Reproduction
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• v.17 no.3
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• pp.249-255
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• 1993

Canine follicular oocytes were used to establish a reliable system for maturation and fertilization in vitro. Ovaries were obtained from either slaughter house or hormone-primed bitches of mixed breeds. The oocytes were recovered by mincing the ovaries in M2＋BSA. Good quality of oocyte-cumulus complexes (OCCs) were selected and cultured in TCM 199 containing 15% fetal calf serum(FCS) for 24~56 h in an atmosphere of 5% CO2 at 39$^{\circ}C$. Maturation rate of follicular oocytes was >87% showing metaphase I. Unlike other domestic animals the cumulus expansion did not occur fully in canine OCCs although minimum expansion was found between the cumulus cells and corona radiata cells, the clear nuclear morphology was presented for the first time by rapid staining. The IVM system used in this study may be useful to obtain fully maturated metaphase I oocyte in dog.

• IMMUNE NETWORK
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• v.15 no.1
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• pp.27-36
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• 2015

In the present study, we investigated the protection conferred by a live attenuated Salmonella enterica serovar Typhimurium (ST) strain against Salmonella Typhimurium, Salmonella Gallinarum (SG), and Salmonella Enteritidis (SE) infection in layer chickens. Birds were orally primed with the attenuated ST strain at 7 days of age and then boosted at 4 weeks post prime immunization (PPI). Sequential monitoring of plasma IgG and mucosal secretory IgA (sIgA) levels revealed that inoculation with ST induced a significant antibody response to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3 Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, and SG strain challenge. Upon virulent SG challenge, the immunized group showed significantly reduced mortality compared to the non-immunized group. The reduced persistence of the virulent ST and SE challenge strains in the liver, spleen, and cecal tissues of the immunized group suggests that immunization with the attenuated ST strain may not only protect against ST infection but can also confer cross protection against SE and SG infection.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.333-337
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• 1998

This study was carried out to get infomation on the nitric oxide production ability and its formation mechanisms of polysaccharides extracted from Ganoderma lucidum(PSG) by using murine macrophage cell line. The cultured mycelial cells of Ganoderma lucidum were extracted by alkali, and than neutralized by acid. The extract were passed through the column of DEAE cellulose for more purification. The neutral fraction was concentrated and precipitated with 95% ethanol. The precipitate was lyophilized and PSG was obtained. The immunomodulating effects of PSG on macrophage were performed by using murine macrophage cell line ATCC TIB 71 cells with PSG 0.5mg. PSG alone could not induce the production of nitrite, but it had a significant potential effect on nitrite secretion when the cells were primed and triggered with BCG and Interferon(IFN)-${\gamma}$. Also it was prominent by using calcium channel blocker(verapamil) and adenylate cyclase activator(forskolin).

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.06a
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• pp.144-146
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• 2011

VTS 항해의 특징은 동적복잡계의 특성을 보인다는 점이다. 자율적인 행위자들인 여러 선박이 서로 영향을 끼치며 상호작용하기 때문에 불확실성이 지배하게 된다. 이러한 불확실성으로 인한 위험을 극복하기 위한 방안으로 본 연구에서는 협력항해(co-navigation)이라는 개념을 중심으로 논의를 전개하였다. 본질적으로 협력항해는 수많은 상황판단과 의사결정들의 집합체이기 때문에 우선 개별 선박들의 상황판단이 어떻게 이루어지는가를 연구하는 것으로부터 출발하여야 한다. 따라서 본고에서는 항해에서 있어서의 상황자각이 어떻게 기능하는지 알아보고, 개인적인 상황판단에서 VTS 전체 차원의 최종 의사결정이 이루어지는 전 과정을 6단계로 세분하여 논의를 전개하였다. 이렇게 세분한 각 단계에서 양질의 인지과업이 이루어지도록 돕고, 이때 저지르게 되는 실수를 수정할 수 있도록 여유시간을 확보할 수 있도록 함으로써 궁극적으로 항해위험을 줄일 수 있다는 것을 이번 연구에서 밝히고자 하였다.

• Asia Marketing Journal
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• v.24 no.4
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• pp.161-168
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• 2023

With the increased mobility within nations, relocation has become a frequent and regular occurrence in modern life. However, we know little about how residential mobility affects purchase type. How does residential mobility affect our purchase behavior? This research explores the relationship between residential mobility and preference of purchase type, with psychological processes. The study primed participants with either a high or low mobility mindset to investigate the effect of mobility on consumption style in U.S. and South Korean. The results of the study confirm that the consideration of residential mobility shifts consumers' preferences toward material goods and experiences. In addition, results also indicate that the connection between mobility and purchase type was mediated by autonomy and moderated by cultural factor. Lastly, the theoretical and practical implications are covered.

• BMB Reports
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• v.56 no.2
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• pp.84-89
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• 2023

The implications of nutrient starvation due to aging on the degeneration of the retinal pigment epithelium (RPE) is yet to be fully explored. We examined the involvement of AMPK activation in mitochondrial homeostasis and its relationship with the maintenance of a healthy mitochondrial population and epithelial characteristics of RPE cells under nutrient starvation. Nutrient starvation induced mitochondrial senescence, which led to the accumulation of reactive oxygen species (ROS) in RPE cells. As nutrient starvation persisted, RPE cells underwent pathological epithelial-mesenchymal transition (EMT) via the upregulation of TWIST1, a transcription regulator which is activated by ROS-induced NF-κB signaling. Enhanced activation of AMPK with metformin decelerated mitochondrial senescence and EMT progression through mitochondrial biogenesis, primed by activation of PGC1-α. Thus, by facilitating mitochondrial biogenesis, AMPK protects RPE cells from the loss of epithelial integrity due to the accumulation of ROS in senescent mitochondria under nutrient starvation.

• International Journal of Stem Cells
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• v.16 no.4
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• pp.448-449
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• 2023

• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.191-199
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• 2002

In this study, eight primers were used to detect genetic variability and phylogenetic relationships among the eighteen Salmonella strains by the arbitrary-primed PCR(AP-PCR) techniques. Five strains of Salmonella typhimurium, four strains of S entertidis, three strains of S choleraeuis, three strains of S gallinarum and three strains of S pullorum were typed by AP-PCR. The number of AP-PCR bands detected per each primer varied from 39 to 52, with an average of 43.6. A total of 349 AP-PCR bands were generated and among them, 185 bands(53.0%) were polymorphic. Among the primers, GEN 703 and GEN 708 primer showed a high level of polymorphism with 0.682 and 0.676, respectively. But GEN 603, GEN 604 and GEN 607 primer showed a low level of polymorphism with 0.404, 0.460 and 0.472, respectively. Therefore, the these primers will be the most effective for AP-PCR analysis of Salmonella strains. The level of polymorphism of S typhimurium CU 2001(0.77) was similar to that of S typhimurium CU 2002(0.77) and lower than those of other strains such as S typhimurium CU 2003(0.63), S typhimurium ATCC 14028(0.50) and S typhimurium CU 2004(0.43). The level of polymorphism of S enteritidis ATCC 13076(0.83) was similar to that of S enteritidis CU 2005(0.83) and lower than those of other strains such as S enteritidis CU 2006(0.63) and S enteritidis CU 2007(0.58). The level of polymorphism of S choleraeuis CU 2009(0.67) was similar to that of S choleraeuis CU 2010(0.67) and higher than those of other strains such as S choleraeuis CU 2008(0.53). The level of polymorphism of S gallinarum CU 2011(0.70) was similar to that of S gallinarum CU 2012(0.70) and higher than those of other strains Such as S gallinarum ATCC 9184(0.60). The level of polymorphism of S pullorum CU 2013(0.80) was similar to that of S pullorum CU 2014(0.80) and higher than those of other strains such as S pullorum No 11(0.53). Therefore, the AP-PCR analysis will be used a powerful tool for estimating genetic variation and phylogenetic relationships among Salmonella strains.