• 한국임상수의학회지
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• 제18권4호
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• pp.380-389
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• 2001

Non-human primates have been increasing in demand as important experimental animals and companion animals, domestically and internationally. The number of non-human primates for these purposes will be much enhanced in the near future. Despite this trend, basic physiological data are scarcely available in these animal species, leading to the difficulty to diagnose diseases when necessary, due to the absence of reference values. Particularly, there is not any report on the total activity of LDH of non-human primates, let alone LDH isoenzyme patterns, in Korea. LDH isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. In this study, total activities and isoenzyme patterns of LDH were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. There were widespread different values of serum total LDH among the non-human primate species experimented in this study. Serum LDH values of common marmosets and crab-eating macaques were 597.5$\pm$243.1 IU/l and 605.3$\pm$312.6 IU/l, respectively, whereas those of Japanese macaque showed 1,209$\pm$473.8 IU/l. Five isoenzyme fractions of LDH were observed in all experimented non-human primates but their ranks and proportions represented different patterns one another. In common marmosets, the percent of fraction for serum LDH1, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ was 13.7$\pm$6.4%, 23.3$\pm$3.6%, 29.2$\pm$5.0%, 9.4$\pm$1.4% and 24.4$\pm$7.5%, respectively. The rank of LDH isoenzymes was LDH$_3$>LDH$_{5}$>LDH$_2$>LDH$_1$>LDH$_4$, in the descending order. For crab-eating macaques, the fraction of serum LDH$_1$, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ occupied 19.5$\pm$12.7%, 25.3$\pm$9.3%, 23.8$\pm$8.1%, 10.2$\pm$2.8% and 21.3$\pm$14.2%, respectively. The order of LDH isoenzymes was LDH$_2$>LDH$_3$>LDH$_{5}$>LDH$_1$>LDH$_4$, from top to down. On the while, in Japanese macaques, the fraction of serum LDH$_1$ to LDH$_{5}$ showed 23.4$\pm$11.8%, 30.5$\pm$4.1%, 17.4$\pm$3.9%, 11.3$\pm$3.7% and 13.8$\pm$5.6%, respectively. The decreasing order indicated LDH$_2$>LDH$_1$>LDH$_3$>LDH$_{5}$>LDH$_4$. In conclusion, values such as LDH and LDH isoenzyme patterns of investigated for the first time from non-human primates reaared in Korea, could be reference values for the optimal diagnosis and therapy of diseases of the corresponding animal species. Other parameters of hematology and blood biochemistry are urgently needed to study for the benefit of our intimate non-human primates.an primates.

• 한국임상수의학회지
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• 제18권4호
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• pp.390-396
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• 2001

CKP isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. There is not any report on the total activity of CPK of non-human primates, let alone CPK isoenzyme patterns, in Korea. In this study, total activities and isoenzyme patterns of CPK were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. We observed remarkable different values of serum total CPK from the primates used in this experiment. Serum CPK activities of Japanese macaques and crab-eating macaques were 275.8$\pm$158.1 IU/l and 396.7$\pm$697.4 IU/l, respectively, whereas those of common marmosets showed much higher value of 618.8$\pm$1,117.6 IU/l. In all common marmosets and crab-eating macaques, only CPK$_3$ ws observed. In five out of eight Japanese macaques, CPK$_3$ was the sole fraction but two animals showed CPK$_1$ and CPK$_3$ isoenzymes, and the remaining one had CPK$_2$ and CPK$_3$ fractions. There were some discrepancies in the pattern and ratio of isoenzyme fractions in Japanese macaques. In conclusion, values such as CPK and CPK isoenzyme patterns of investigated for the first time form non-human primates reared in Korea, could be reference values for the optimal diagnosis and therapy diseases of the corresponding animal species.

• 생명과학회지
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• 제12권6호
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• pp.676-681
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• 2002

긴팔원숭이는 영장목, 유인원 아목, 좁은코원숭이 상과, 사람과에 속하는 영장류로써 소형 영장류라고 불린다. 이들은 동남 아시아 일대의 열대 우림에서 종 분화에 성공한 영장류로써 현재 4속 12종이 동정 되어 있지만 이들 중 10종이 멸종 위기에 있다. 다양한 영장류 중에 가장 높은 멸종 위기에 있으면서도 아직도 정확하게 많은 종 또는 아종의 동정이 되지 않고 있는 실정이며 많은 연구가 필요한 종이다. 그러므로 정확한 지리학적 분포와 종의 동정을 통해 이들 긴팔원숭이의 계통 관계를 파악 하는 것이 긴팔원숭이의 종분화의 이해 및 보존을 위해 무엇 보다 중요하다고 사료된다.

• Clinics in Shoulder and Elbow
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• 제18권1호
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• pp.52-58
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• 2015

Animal shoulder models are important in the investigation of the natural history of various pathologic conditions and for evaluation of the effectiveness of different treatments and biomaterials. The characteristics of animal shoulder models that may be used for rotator cuff related experiments are different with regard to the anatomy, behavioral pattern, advantages and disadvantages in application to each other. The lower primates and all the non-primate species, except the tree kangaroo, were neither functional overhead nor had a true rotator cuff. Utilizing more advanced primates, or perhaps even the tree kangaroo would be ideal and the most relevant to man as they possess a true rotator cuff. However, ethical concerns, costs, and many limitations in obtaining primates generally preclude the use of these animals for such research. Finally we should consider the differences in comparative anatomy and behavioral pattern of each animal model during performance or interpretation of rotator cuff experiments.

• 한국임상수의학회지
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• 제25권4호
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• pp.314-316
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• 2008

급성 위확장은 실험시설에서 사육되고 있는 Macaca 원숭이에서 가장 흔하게 발생하는 응급상황 중에 하나이다. 이 보고서는 사육 원숭이에서 발생한 몇몇 증례를 소개하고자 한다. 급성 위확장증을 보인 원숭이들에는 실험을 위해 원숭이 보정의자에 앉힌 경우나 마취를 한 경우, 그리고 아무런 처치도 하지 않은 경우 등이 있었다. 이환된 동물들은 심한 복부팽만, 탈수, 청색증과 호흡곤란 등을 동반한 혼수 상태를 보였다. 한 증례는 전신장애로 인해 상태가 악화되고 폐사하여 부검을 실시하였다. 나머지 두 증례는 위관과 수액요법으로 응급처치를 한 결과 병증에서 회복되었다. 부검결과, 위의 대부분은 위내 가스와 물 그리고 섭취물로 채워져 있었다. 이 보고서는 영장류에서 급성위확장증의 발생과 관리에 대한 특별한 강조와 더불어 본 질환이 비 특이적인 원인에 의해 발생할 수 도 있음을 시사한다.

• Molecules and Cells
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• 제26권1호
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• pp.53-60
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• 2008

We characterized the human endogenous retrovirus (HERV-W) family in humans and primates. In silico expression data indicated that 22 complete HERV-W families from human chromosomes 1-3, 5-8, 10-12, 15, 19, and X are randomly expressed in various tissues. Quantitative real-time RT-PCR analysis of the HERV-W env gene derived from human chromosome 7q21.2 indicated predominant expression in the human placenta. Several copies of repeat sequences (SINE, LINE, LTR, simple repeat) were detected within the complete or processed pseudo HERV-W of the human, chimpanzee, and rhesus monkey. Compared to other regions (5'LTR, Gag, Gag-Pol, Env, 3'LTR), the repeat family has been mainly integrated into the region spanning the 5'LTRs of Gag (1398 bp) and Pol (3242 bp). FISH detected the HERV-W probe (fosWE1) derived from a gorilla fosmid library in the metaphase chromosomes of all primates (five hominoids, three Old World monkeys, two New World monkeys, and one prosimian), but not in Tupaia. This finding was supported by molecular clock and phylogeny data using the divergence values of the complete HERV-W LTR elements. The data suggested that the HERV-W family was integrated into the primate genome approximately 63 million years (Myr) ago, and evolved independently during the course of primate radiation.

• Journal of Life Science
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• 제10권1호
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• pp.37-39
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• 2000

Testis-specific protein Y(TSPY) is thought to play an important role during spermatogenesis in primates. The Arg and Lys-rich region of TSPY was implicated as a potential DNA binding site in the human and Japanese monkey. In the study, we investigated this further through looking at the putative amino acid sequences of Arg and Lys-rich region of TSPY gene from seven species of gibbons, five species of Old World monkeys and five species of New World monkeys. A comparison with those of the human and Japanese monkey revealed that the Arg and Lys-rich motif was hightly conserved in various primates. This finding suggests that a possible role for the Arg and Lys-rich motif in primate TSPY is DNA recognition. The functional implications for TSPY are discussed in the light of this and previous findings.

• 대한수의학회지
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• 제19권1호
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• pp.1-8
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• 1979

Detailed human gross anatomic structures have been characterized. No similar data are available in nonhuman primate species in spite of close phylogenic similarity found between man and nonhuman primates. The ever increasing incidence of lung cancer and air pollution related respiratory ailments found in man emphasizes the need for an ideal animal model for studying pathogenesis of these various human pulmonary diseases. Thus, detailed investigation of pulmonary structures found in various species of nonhuman primates is warranted. For determining primate gross pulmonary anatomic structure, published works concerning the number of tracheal cartilage, angle of tracheal bifurcation, caliber of trachea, lung lobe and bifurcation position of trachea recorded for several species of nonhuman pimates, were reviewed. Limited information is available concerning the number of tracheal cartilage, width of tracheal cartilage, angle of bronchus, caliber of trachea and bronchus, and the bifurcation position of the trachea including the length of bronchus on nonhuman primates. Since scanty data have been gathered with no specific reference to their age, sex and body weight, they have no comparative values.

• 한국임상수의학회지
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• 제19권2호
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• pp.132-138
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• 2002

Non-human primates are widely used for experimental animal and raised as companion animal in Korea. To establish the electrocadiogram (ECG) of non-human primates that are domestically raised, the author measured bipolar limb leads and augmented unipolar limb leads, after tiletamine/zolazepam (TZ) injection as sedative agents. The amplitudes of P,Q, R,S and T wave and duration time of P wave, QRS complex, PR and QT interval in each lead of ECG were evaluated in 7 non-human primates at 15 minutes after TZ injection, respectively. The amplitude of P wave in I, II,III, aVR, aVL and aVF leads revealed 0.06$\pm$ 0.05,0.14$\pm$ 0.05, 0.1 $\pm$ 0.05,-0.11 $\pm$ 0.06,-0.04$\pm$ 0.04 and 0.12$\pm$ 0.05 mV respectively. The amplitude of Q wave revealed -0.16$\pm$ 0.15, -0.23$\pm$ 0.18, -0.17$\pm$ 0.13, 0.16$\pm$0.13, 0.04$\pm$ 0.09 and -0.2$\pm$0.13 mV, respectively. The amplitude of R wave revealed 0.56$\pm$0.56, 1.24$\pm$ 0.67, 0.92$\pm$0.33, -0.37$\pm$ 1.14, -0.22$\pm$ 0.47 and 1.12 $\pm$ 0.47 mV, respectively. The amplitude of S wave revealed -0.02$\pm$ 0.08, -0.04$\pm$0.06, -0.06$\pm$0.04, 0.02$\pm$0.04, 0.04$\pm$0.09 and -0.04 $\pm$ 0.06 mV, respectively. The amplitude of T wave revealed -0.01 $\pm$ 0.15,-0.02$\pm$ 0.13, 0.01 $\pm$ 0.08, 0.02$\pm$ 0.12, 0.01 $\pm$ 0.11 and -0.03$\pm$ 0.09 mV, respectively. The duration time of P wave revealed 0.05 $\pm$ 0.01, 0.04$\pm$ 0.01, 0.05$\pm$ 0.02, 0.05 $\pm$ 0.02, 0.04$\pm$ 0.01 and 0.04$\pm$ 0.01 sec, respectively. The duration time of QRS complex revealed 0.05 $\pm$ 0.02,0.05$\pm$ 0.01, 0.05 $\pm$ 0.01, 0.04$\pm$ 0.01, 0.05$\pm$ 0.01 and 0.05 $\pm$ 0.01 sec, respectively. The duration time of PR interval revealed 0.08$\pm$ 0.01, 0.07$\pm$0.01,0.08$\pm$ 0.03, 0.08$\pm$0.01, 0.08$\pm$ 0.01 and 0.08$\pm$0.01 sec, respectively. The duration time of QT interval revealed 0.23$\pm$ 0.06, 0.22$\pm$ 0.05, 0.23 $\pm$ 0.06, 0.23$\pm$ 0.06, 0.24$\pm$ 0.05 and 0.22$\pm$ 0.02 sec, respectively. No significant changes were observed in e amplitude of P and T waves. The amplitude of QRS complex in ketamine group was higher than that of TZ group. However, no significant changes were observed in both intra-group and inter-group. There were no significant changes in the duration time of P wave, QRS complex and PR interval obtained from both groups. Also, the duration time of QT interval in TZ group was significantly longer at 30 min.(P< 0.05) an that of 10 minutes after injection. However, significant difference was not found between two groups. The mean cardiac electric axis in ketamine group tended to decrease until 30 min. after injection and then gradually increase. However, mean cardiac electric axis of TZ group was increased until 30 min. after injection and then decreased. But significant differences were not observed between groups. These results suggest that the ECG pattern after TZ injection to non-human primates reared in korea was established. It was also considered that the injection of ketamine and TZ didn't significantly affect on ECG pattern of the non-human primates.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 1996년도 추계 학술대회 및 정기총회
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• pp.65-66
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• 1996