• 제목/요약/키워드: primary phase

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한국재래산양(韓國在來山羊)의 정자발생(精子發生)에 관한 연구(硏究) (Studies on Spermatogenesis in Korean Native Goat)

  • 이재홍;이방환;이성호
    • 대한수의학회지
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    • 제25권2호
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    • pp.91-101
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    • 1985
  • This study was conducted in order to observe the changes in cellular associations of seminiferous tubules from 8 to 20 weeks of age and to obtain the cycle and relative duration of the seminiferous epithelia from 24 to 32 weeks of age. Twenty-eight Korean native male goats were used in the experiment and divided into 7 groups, consisting of 4 goats each, with four weeks intervals from 8 to 32 weeks of age. The results were summarized as follows; 1. Gonocytes were seen at 8 weeks of age, however they were not observed as from 12 weeks. Both type A-spermatogonia and type B-spermatogonia occurred from 8 weeks, while primary spermatocytes were found from 12 weeks. Secondary spermatocytes and spermatids appeared from 16 weeks, and increased in numbers sequentially until 32 weeks of age. Spermatozoa were observed at first at 20 weeks of age. 2. Type A-spermatogonia appeared approximately twice as many at stage 2 as compared to stage 1, while the same numbers of cells were seen in both stages 1 and 8, showing the least number among 8 stages of the cycle of the seminiferous epithelia. The type B-spermatogonia were found during the stage 5 to 8, not to be detactable during stage 1 to 4. The number of primary spermatocytes of the leptotene phase increased markedly during stage 1 to 4, and decreased afterwards. The primary spermatocytes of the pachytene phase were shown the least in number at stage 4. The secondary spermatocytes could be seen only at stage 4 and the largest number of spermatids was seen at the stage 4 among 8 stages. 3. The relative frequencies of each stage among stages 1 to 8 of the cycle of the seminiferous epithelia were 27.5, 17.5, 12.8, 5.8, 8.9, 8.3, 12.0 and 7.2% respectively. 4. Some of the nuclei of Sertoli cells transformed from the "parallel" type to the "perpendicular" type. This evolution took place from stage 1 to 6, when the number of "perpendicular" type nuclei reached a peak and the number was decreased in the rest of the stages. Thus, establishment of spermatogenesis in Korean native goats was completed at the age of 20 weeks.

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리모델링시 학교시설의 친환경건축물 인증요소 비교분석 연구 (A Study on the Comparative Analysis of the Green Building Certification Criteria for Schools in remodeling)

  • 양원석;주범
    • 한국실내디자인학회논문집
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    • 제20권1호
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    • pp.182-189
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    • 2011
  • This study is purposed on applying to certifying environmental-friendly buildings about remodeling schools as an issue recently, keeping up a worldwide resource conservation movement. Application standard is directed, comparing with systems certifying green-schools in domestic and foreign policies. The following is the primary result of this study. First, on developing systems for certifying environmental-friendly buildings about remodeling schools, characteristics like ‘the phase of certifying essential lists and the diversity of certifying lists’, ‘developing departmentally into new-building, reconstruction, remodeling, existing-building in basic data’, ‘development of certifying existing-buildings as well as new-building’ in foreign systems should be developed appropriately for Korea. Next, not only reuse of the existing facilities but better assessment through diversification of classified contents in domestic system are carried out in comparing remodeling lists. Especially the primary list that is found out commonly limited lists certifying remodeling should be developed appropriately. Impossible lists getting certification should be applied to map out a concrete standard. Lastly, the range of certification should be diversified and developed sustainable like other systems for better assessment. Adding various lists suiting for Korea and departmentalizing score of phases and items in particular, for avoiding a needless collision between certification lists, absence of them. Above of all, basic direction about certifying environmental-friendly buildings about remodeling schools should be developed the only individual instrument, not for gaining it simply as a part of the environmental policy in Korea. This study has significance on vitalization of certifying environmental-friendly buildings, which performing primary environmental-friendly education facilities, based on its advance.

침상구조의 루틸상 TiO2 초미분체를 이용한 광촉매 반응에 대한 연구 (Study on Photocatalytic Reaction Using Acicular TiO2 Rutile Powder)

  • 황두선;구숙경;김광수;민형섭;이은구;김선재
    • 한국재료학회지
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    • 제12권8호
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    • pp.641-649
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    • 2002
  • The redox properties of a homogeneously-precipitated $TiO_2$ rutile powder with a BET surface area of ~$200 m^2$/g, consisting of an acicular primary particle, were characterized using photocatalytic reaction in aqueous 4-chlorophenol, Cu-EDTA and Pb-EDTA solutions under ultraviolet irradiation, compared to those of commercial P-25 X$200 m_2$ powder with a spherical primary particle as well as home-made anatase $TiO_2$ powder with ~$200 m^2$/g BET surface area. Here, the anatase powder also includes mainly the primary particles very similar to the acicular shapes of the rutile $TiO_2$ powder. The rutile powder showed the fastest decomposition rate and the largest amount in the photoredor, compared with the anatase or P-25 powder, while the anatase powder unexpectedly showed the slowest rate and the smallest amount in the same experiments regardless of almost the same surface area. From results, the excellent photoredox abilities of this rutile powder appears to be due to specific powder preparation method, like a homogeneous precipitation leading to direct crystallization from the solution, regardless of their crystalline structures even when having the similar particle shape and surface area.

수종의 암세포주와 치은섬유아세포에서 방사선의 양과 분할조사에 따른 세포활성도와 독성의 변화에 관한 연구 (AN EXPERIMENTAL STUDY ON THE CHANGE OF THE RADIOSENSITIVITY OF SEVERAL TUMOR CELL LINES AND PRIMARY CULTURED GINGIVAL FIBROBLAST)

  • 이삼선;유동수
    • 치과방사선
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    • 제27권1호
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    • pp.107-122
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    • 1997
  • Radiation sensitivity data was generated for two human cancer cell lines(KB, RPMI 2650) and human primary gingival fibroblast was tested three times using a viable cell number counting with a hemocytometer, MTT(3-[4,5-Dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide) assay, and LDH(Lactate dehydrogenase) assay. Single irradiation of 2, 4, 6, 10, 15, 20Gy were applied to the tumor cell lines and the primary cultured gingival fibroblast The two fractions of 4Gy and 10Gy were seperated with a 4 hour time interval. The irradiation was done with 241.5cGy/min dose rate using /sup 137/Cs MK cell irradiator at room temperature. The obtained results were as followed : 1. There was significantly different viable cell numbers as the amount of radiation dose on the tested cells were cell number counted with a hemocytometer. In fractions, there were more viable cells remaining. 2. Phase-contrast microscopically, radiation-induced morphologic changes were pronounced on the tumor cells, however, almost no differences on the gingival fibroblast. 3. There was significantly different absorbance at 2Gy on RPMI 2600, 4Gy on KB and GF in MTT assay. In fractions, the absorbance was significantly higher on KB. 4. The level of extracellular LDH activity in the experimental group was significantly higher in the 2-4Gy than the control group. 5. The total level of extracellular and intracellular LDH activity was decreased as increased amounts of radiation dose was applied.

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협력적 스펙트럼 공유의 자동 반복 프로토콜 (An Automatic Repeating Protocol in Cooperative Spectrum Sharing)

  • 공형윤
    • 한국인터넷방송통신학회논문지
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    • 제13권6호
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    • pp.101-108
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    • 2013
  • 본 논문에서는, NACK 메시지가 스펙트럼 공유와 협력을 위한 명령으로 사용되어지는 방법을 제안하였다. 1차 사용자의 직접 연결이 중단될 때, 협력과 공유의 자동 반복을 허가하고, 패러다임 기반의 협력 스펙트럼 공유의 조정 메시지의 수를 절약하기 위해 허가한다. 공유 시, ${\alpha}$의 남은 전력 부분이 1차 재전송 신호인 동안, 2차 전송된 신호를 위한 $1-{\alpha}$ 전력 부분은 중계기 공유로 선택된다. 중계기를 사용하지 않는 경우, 1차 송신단은 전체 전력($1-{\alpha}$)를 사용하여 신호를 재전송 하기 위해 NACK를 사용한다. 두 시스템은 BPSK 신호를 적용한 것으로 가정한다. 이 기법에서, 2차 사용자는 공동 최적화 복호를 하는 것으로 제안한다. 프레임 오류율(FER) 성능은 양 시스템에서 분석된다. 이론과 시뮬레이션 결과는 본 프로토콜의 유효성을 분석하고 효율적임을 확인하였다.

HVOF 용사된 $\textrm{Cr}_{3}\textrm{C}_{2}$-NiCr 용사층의 특성 (Characteristics of the HVOF_sprayed $\textrm{Cr}_{3}\textrm{C}_{2}$-NiCr Coationg Layer)

  • 김병희;서동수
    • 한국재료학회지
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    • 제8권9호
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    • pp.849-855
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    • 1998
  • 20wt%NiCr이 크래드된 크롬카바이드 분말과 7wt%NiCr이 기계적으로 혼합된 크롬카바이드 분말을 이용하여 HVOF 용사된 용사층의 특성(미세조직, 결정상, 경도값 그리고 erosion rate)을 비교하였다. 용사상태의 미세조직강의 특성은 크래드분말의 경우에 primary $\textrm{Cr}_{3}\textrm{C}_{2}$상이 용사층에는 남아 있었으나 혼합분말의 경우에는 primary $\textrm{Cr}_{3}\textrm{C}_{2}$ 상은 용사층에 거의존재하지 않았다. 또한 XRD 분석결과 두 분말 모두 용사과정에서 크롬카바이드의 분해는 일어났으나 분해율은 크래드분말의 경우가 혼합분말보다 낮았다. 용사상태에서 경도값은 혼합분말의 경우가 높았으며 $1000^{\circ}C$까지 열처리 후 혼합분말의 경도값은 1665까지 증가하였으나 크래드분말은 $600^{\circ}C$를 정점으로 감소하는 경향을 보였다.

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신경세포가 별아교세포의 아교섬유성 산단백질 표현에 미치는 영향 (Effect of Glial-neuronal Cell Co-culture on GFAP Expression of Astrocytes)

  • 배형미;박정선;연동수
    • The Korean Journal of Physiology and Pharmacology
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    • 제1권3호
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    • pp.285-296
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    • 1997
  • Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

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하수 슬러지 건조장치 운전시 하수슬러지 함수율 및 조성 분석연구 (The study of sewage sludge moisture content and composition analysis for sewage sludge dryer)

  • 이건주
    • 유기물자원화
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    • 제22권3호
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    • pp.53-59
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    • 2014
  • 본 연구에서는 A 지역의 하수슬러지 건조장치가 설치되기 전과 후 탈수슬러지의 조성 및 성상, 중금속함유량 등을 측정 및 조사 분석을 하였다. 분석결과 건조로 설치전보다 약 95.7%의 제거효율을 보였으며, 설계값으로 1차건조로 35%, 2차건조로 10% 함수율을 제거하지만, 측정해본결과 1차건조로 20.8%, 리본 3.3%로 실제 운전효과가 더 좋은 것으로 나타났다. 중금속 함유량은 건조장치 설치 전 Cu : 352~614ppm, Hg : 1.3~1.44ppm, Cd : 1.1~1.86ppm, Pb : 17.25~ 28.93 ppm, As : 1ppm 이고 설치 후 Cu : 340~350 ppm, Hg : 0 ppm, Cd : 0~0.021 ppm, Pb : 0 ppm, As : 0~0.043 ppm으로 건조장치설치 전과 후 모두 법정 기준치에 초과하지 않음을 확인하였다. 또한 건조장치를 통과한 하수슬러지는 평균적으로 ${\frac{1}{4}}$의 건조슬러지가 발생하였다.

일차성 간림프종: 자기공명영상과 병리소견의 연관 (Primary Hepatic Lymphoma: MR Imaging and Pathologic Correlation)

  • 김한나;신유리;나성은;정은선;오순남;최준일;정승은;이영준
    • Investigative Magnetic Resonance Imaging
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    • 제14권2호
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    • pp.151-155
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    • 2010
  • 일차성 간림프종은 전체 림프절외장기를 침범하는 림프종의 1% 미만에서 나타나는 매우 드문 질환이다. 저자들은 수술로 확진된 일차성 간림프종의 자기공명영상과 병리 소견의 특징을 보고한다. 간세포 특이 조영제를 주입하여 얻은 역동적 조영증강 영상에서 점차적으로 조영증강되어 간내담관암과 유사하게 보였다. 그러나 20분 지연 후 얻은 간담관기 영상과 확산강조영상에서 서로 다른 신호강도를 보이는 세 층의 띠모양의 병변이 특징적으로 보였으며 이는 병리적으로 외층의 생존 종양, 중간층의 괴사된 종양, 및 종양에 둘러싸인 중앙부의 괴사된 간 실질과 일치되는 소견을 보였다.

도시지역 낮.밤 대기에어로졸의 입경 별 수용성 유기탄소의 특성 (Characteristic of Size-Resolved Water-Soluble Organic Carbon in Atmospheric Aerosol Particles Observed during Daytime and Nighttime in an Urban Area)

  • 박승식;신동명
    • 한국입자에어로졸학회지
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    • 제9권1호
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    • pp.7-21
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    • 2013
  • Twelve-hour size-resolved atmospheric aerosols were measured to determine size distributions of water-soluble organic carbon(WSOC) during daytime and nighttime, and to investigate sources and formation pathways of WSOC in individual particle size classes. Mass, WSOC, ${NO_3}^-$, $K^+$, and $Cl^-$ at day and night showed mostly bimodal size distributions, peaking at the size range of $0.32-0.55{\mu}m$(condensation mode) and $3.1-6.2{\mu}m$(coarse mode), respectively, with a predominant condensation mode and a minor coarse mode. While ${NH_4}^+$ and ${SO_4}^{2-}$ showed unimodal size distributions which peaked between 0.32 and $0.55{\mu}m$. WSOC was enriched into nuclei mode particles(< $0.1{\mu}m$) based on the WSOC-to-mass and WSOC-to-water soluble species ratios. The sources and formation mechanisms of WSOC were inferred in reference to the size distribution characteristics of inorganic species(${SO_4}^{2-}$, ${NO_3}^-$, $K^+$, $Ca^{2+}$, $Na^+$, and $Cl^-$) and carbon monoxide. Nuclei mode WSOC was likely associated with primary combustion sources during daytime and nighttime. Among significant sources contributing to the condensation mode WSOC were homogeneous gas-phase oxidation of VOCs, primary combustion emissions, and fresh(or slightly aged) biomass burning aerosols. The droplet mode WSOC could be attributed to aqueous oxidation of VOCs in clouds, cloud-processed biomass burning aerosols, and small contributions from primary combustion sources. From the correlations between WSOC and soil-related particles, and between WSOC and sea-salt particles, it is suggested that the coarse mode WSOC during daytime is likely to condense on the soil-related particles($K^+$ and $Ca^{2+}$), while the WSOC in the coarse fraction during nighttime is likely associated with the sea-salt particles($Na^+$).