• Title/Summary/Keyword: primary phase

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Studies on Spermatogenesis in Korean Native Goat (한국재래산양(韓國在來山羊)의 정자발생(精子發生)에 관한 연구(硏究))

  • Lee, Jae-hong;Lee, Bang-whan;Lee, Seong-ho
    • Korean Journal of Veterinary Research
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    • v.25 no.2
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    • pp.91-101
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    • 1985
  • This study was conducted in order to observe the changes in cellular associations of seminiferous tubules from 8 to 20 weeks of age and to obtain the cycle and relative duration of the seminiferous epithelia from 24 to 32 weeks of age. Twenty-eight Korean native male goats were used in the experiment and divided into 7 groups, consisting of 4 goats each, with four weeks intervals from 8 to 32 weeks of age. The results were summarized as follows; 1. Gonocytes were seen at 8 weeks of age, however they were not observed as from 12 weeks. Both type A-spermatogonia and type B-spermatogonia occurred from 8 weeks, while primary spermatocytes were found from 12 weeks. Secondary spermatocytes and spermatids appeared from 16 weeks, and increased in numbers sequentially until 32 weeks of age. Spermatozoa were observed at first at 20 weeks of age. 2. Type A-spermatogonia appeared approximately twice as many at stage 2 as compared to stage 1, while the same numbers of cells were seen in both stages 1 and 8, showing the least number among 8 stages of the cycle of the seminiferous epithelia. The type B-spermatogonia were found during the stage 5 to 8, not to be detactable during stage 1 to 4. The number of primary spermatocytes of the leptotene phase increased markedly during stage 1 to 4, and decreased afterwards. The primary spermatocytes of the pachytene phase were shown the least in number at stage 4. The secondary spermatocytes could be seen only at stage 4 and the largest number of spermatids was seen at the stage 4 among 8 stages. 3. The relative frequencies of each stage among stages 1 to 8 of the cycle of the seminiferous epithelia were 27.5, 17.5, 12.8, 5.8, 8.9, 8.3, 12.0 and 7.2% respectively. 4. Some of the nuclei of Sertoli cells transformed from the "parallel" type to the "perpendicular" type. This evolution took place from stage 1 to 6, when the number of "perpendicular" type nuclei reached a peak and the number was decreased in the rest of the stages. Thus, establishment of spermatogenesis in Korean native goats was completed at the age of 20 weeks.

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A Study on the Comparative Analysis of the Green Building Certification Criteria for Schools in remodeling (리모델링시 학교시설의 친환경건축물 인증요소 비교분석 연구)

  • Yang, Won-Seok;Chu, Beom
    • Korean Institute of Interior Design Journal
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    • v.20 no.1
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    • pp.182-189
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    • 2011
  • This study is purposed on applying to certifying environmental-friendly buildings about remodeling schools as an issue recently, keeping up a worldwide resource conservation movement. Application standard is directed, comparing with systems certifying green-schools in domestic and foreign policies. The following is the primary result of this study. First, on developing systems for certifying environmental-friendly buildings about remodeling schools, characteristics like ‘the phase of certifying essential lists and the diversity of certifying lists’, ‘developing departmentally into new-building, reconstruction, remodeling, existing-building in basic data’, ‘development of certifying existing-buildings as well as new-building’ in foreign systems should be developed appropriately for Korea. Next, not only reuse of the existing facilities but better assessment through diversification of classified contents in domestic system are carried out in comparing remodeling lists. Especially the primary list that is found out commonly limited lists certifying remodeling should be developed appropriately. Impossible lists getting certification should be applied to map out a concrete standard. Lastly, the range of certification should be diversified and developed sustainable like other systems for better assessment. Adding various lists suiting for Korea and departmentalizing score of phases and items in particular, for avoiding a needless collision between certification lists, absence of them. Above of all, basic direction about certifying environmental-friendly buildings about remodeling schools should be developed the only individual instrument, not for gaining it simply as a part of the environmental policy in Korea. This study has significance on vitalization of certifying environmental-friendly buildings, which performing primary environmental-friendly education facilities, based on its advance.

Study on Photocatalytic Reaction Using Acicular TiO2 Rutile Powder (침상구조의 루틸상 TiO2 초미분체를 이용한 광촉매 반응에 대한 연구)

  • Hwang, Doo-Sun;Ku, Suk-Kyeong;Kim, Kwang-Su;Min, Hyung-Seob;Lee, Eun-Gu;Kim, Sun-Jae
    • Korean Journal of Materials Research
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    • v.12 no.8
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    • pp.641-649
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    • 2002
  • The redox properties of a homogeneously-precipitated $TiO_2$ rutile powder with a BET surface area of ~$200 m^2$/g, consisting of an acicular primary particle, were characterized using photocatalytic reaction in aqueous 4-chlorophenol, Cu-EDTA and Pb-EDTA solutions under ultraviolet irradiation, compared to those of commercial P-25 X$200 m_2$ powder with a spherical primary particle as well as home-made anatase $TiO_2$ powder with ~$200 m^2$/g BET surface area. Here, the anatase powder also includes mainly the primary particles very similar to the acicular shapes of the rutile $TiO_2$ powder. The rutile powder showed the fastest decomposition rate and the largest amount in the photoredor, compared with the anatase or P-25 powder, while the anatase powder unexpectedly showed the slowest rate and the smallest amount in the same experiments regardless of almost the same surface area. From results, the excellent photoredox abilities of this rutile powder appears to be due to specific powder preparation method, like a homogeneous precipitation leading to direct crystallization from the solution, regardless of their crystalline structures even when having the similar particle shape and surface area.

AN EXPERIMENTAL STUDY ON THE CHANGE OF THE RADIOSENSITIVITY OF SEVERAL TUMOR CELL LINES AND PRIMARY CULTURED GINGIVAL FIBROBLAST (수종의 암세포주와 치은섬유아세포에서 방사선의 양과 분할조사에 따른 세포활성도와 독성의 변화에 관한 연구)

  • Lee Sam-Sun;You Dong-Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.27 no.1
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    • pp.107-122
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    • 1997
  • Radiation sensitivity data was generated for two human cancer cell lines(KB, RPMI 2650) and human primary gingival fibroblast was tested three times using a viable cell number counting with a hemocytometer, MTT(3-[4,5-Dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide) assay, and LDH(Lactate dehydrogenase) assay. Single irradiation of 2, 4, 6, 10, 15, 20Gy were applied to the tumor cell lines and the primary cultured gingival fibroblast The two fractions of 4Gy and 10Gy were seperated with a 4 hour time interval. The irradiation was done with 241.5cGy/min dose rate using /sup 137/Cs MK cell irradiator at room temperature. The obtained results were as followed : 1. There was significantly different viable cell numbers as the amount of radiation dose on the tested cells were cell number counted with a hemocytometer. In fractions, there were more viable cells remaining. 2. Phase-contrast microscopically, radiation-induced morphologic changes were pronounced on the tumor cells, however, almost no differences on the gingival fibroblast. 3. There was significantly different absorbance at 2Gy on RPMI 2600, 4Gy on KB and GF in MTT assay. In fractions, the absorbance was significantly higher on KB. 4. The level of extracellular LDH activity in the experimental group was significantly higher in the 2-4Gy than the control group. 5. The total level of extracellular and intracellular LDH activity was decreased as increased amounts of radiation dose was applied.

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An Automatic Repeating Protocol in Cooperative Spectrum Sharing (협력적 스펙트럼 공유의 자동 반복 프로토콜)

  • Kong, Hyung-Yun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.6
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    • pp.101-108
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    • 2013
  • In this paper, we propose a method in which the negative acknowledge (NACK) message is used as command for cooperation and spectrum sharing. This allows for an automatic request for cooperation and sharing when the direct link of the primary user is in outage, and also allows for saving the number of control messages in cooperation-spectrum sharing based paradigm. In the sharing phase, the selected relay shares a power fraction of $1-{\alpha}$ for secondary transmitted signal while the remaining of ${\alpha}$ is for primary retransmitted signal. In the case of no relay collected, primary transmitter uses NACK as a command to retransmit the signal with fully power fraction (${\alpha}=1$). Both systems are assumed to employ BPSK signals. In this scheme, we propose the joint optimal decoding in the secondary user. The frame error rate (FER) performance at both systems is then analyzed. The theoretical and simulation results validate the analysis and confirm the efficiency of the protocol.

Characteristics of the HVOF_sprayed $\textrm{Cr}_{3}\textrm{C}_{2}$-NiCr Coationg Layer (HVOF 용사된 $\textrm{Cr}_{3}\textrm{C}_{2}$-NiCr 용사층의 특성)

  • Kim, Byeong-Hui;Seo, Dong-Su
    • Korean Journal of Materials Research
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    • v.8 no.9
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    • pp.849-855
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    • 1998
  • The purpose of this study was performed to compare to the characteristics (microstructure, phase change and hardness, erosion rate) of HVOF sprayed coatings with 20wt% NiCr claded and 7wt%NiCr mixed $\textrm{Cr}_{3}\textrm{C}_{2}$ powder. In the case of the 20wt% NiCr claded $\textrm{Cr}_{3}\textrm{C}_{2}$ powder, microstructural feature showed that the primary $\textrm{Cr}_{3}\textrm{C}_{2}$ was remained in the coating but was barely remained in the mixed $\textrm{Cr}_{3}\textrm{C}_{2}$ coating. As a results of XRD analysis, both 20wt%NiCr claded and 7wt% NiCr mixed $\textrm{Cr}_{3}\textrm{C}_{2}$ powder was decomposed during spraying but the degree of decomposition of the 20wt%NiCr claded was lower than 7wt%NiCr mixed $\textrm{Cr}_{3}\textrm{C}_{2}$ powder. After spraying the mixed powder for microhardness was higher than claded $\textrm{Cr}_{3}\textrm{C}_{2}$ powder and which was increased up to $\textrm{Hv}_{300}$= 1665 after heat treatment to $1000^{\circ}C$. however. 20wt%NiCr claded $\textrm{Cr}_{3}\textrm{C}_{2}$ became to decrease at $600^{\circ}C$ which was the maximum.

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Effect of Glial-neuronal Cell Co-culture on GFAP Expression of Astrocytes (신경세포가 별아교세포의 아교섬유성 산단백질 표현에 미치는 영향)

  • Bae Hyung-Mi;Park Jung-Sun;Yeon Dong-Soo
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.3
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    • pp.285-296
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    • 1997
  • Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

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The study of sewage sludge moisture content and composition analysis for sewage sludge dryer (하수 슬러지 건조장치 운전시 하수슬러지 함수율 및 조성 분석연구)

  • Lee, Keon Joo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.22 no.3
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    • pp.53-59
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    • 2014
  • In this study, the sewage sludge dryer is installed before and after dehydration of the sludge and aqueous phase composition, the heavy metal content, and measurement and analysis were investigated. The removal efficiency of water content of sewage sludge was about 95.7% in the sewage sludge dryer. The removal efficiency of water content for primary dryer was designed for 35% of primary drying, secondary drying to remove the water content to 10%, but as the measurement revealed that 20.8% of primary drying, the second dryer 3.3% a better effect to the actual operation respectively. Before the installation of the sewage sludge dryer, the content of heavy metal was as follows, Cu:352~614 ppm, Hg: 1.3~1.44ppm, Cd : 1.1~1.86ppm, Pb : 17.25~ 28.93 ppm, As : 1ppm. And after the installation of the sewage sludge dryer, the content of heavy metal changed to as follows, Cu : 340~350 ppm, Hg : 0 ppm, Cd : 0~0.021 ppm, Pb : 0 ppm, As : 0~0.043 ppm which is Also below the legal limits. Also, the sewage sludge dryer produce 1/4 of the sewage sludge into dried sludge.

Primary Hepatic Lymphoma: MR Imaging and Pathologic Correlation (일차성 간림프종: 자기공명영상과 병리소견의 연관)

  • Kim, Han-Na;Shin, Yu-Ri;Rha, Sung-Eun;Jung, Eun-Sun;Oh, Soon-Nam;Choi, Joon-Il;Jung, Seung-Eun;Lee, Young-Joon
    • Investigative Magnetic Resonance Imaging
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    • v.14 no.2
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    • pp.151-155
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    • 2010
  • Primary hepatic lymphoma is extremely rare, representing less than 1% of all extranodal lymphomas. We report MR imaging features and pathologic correlation of a case of primary hepatic lymphoma. MR images showed a large lobulated mass with gradual contrast enhancement, resembling intrahepatic cholangiocarcinoma. However, both hepatobiliary phase image obtained 20 minutes after injection of hepatocyte specific contrast agent and diffusion-weighted image demonstrated characteristic three layered pattern representing viable lymphoma in the outer layer, tumor necrosis in the middle layer and necrotic hepatic parenchyma in the center.

Characteristic of Size-Resolved Water-Soluble Organic Carbon in Atmospheric Aerosol Particles Observed during Daytime and Nighttime in an Urban Area (도시지역 낮.밤 대기에어로졸의 입경 별 수용성 유기탄소의 특성)

  • Park, Seung Shik;Shin, Dong Myung
    • Particle and aerosol research
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    • v.9 no.1
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    • pp.7-21
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    • 2013
  • Twelve-hour size-resolved atmospheric aerosols were measured to determine size distributions of water-soluble organic carbon(WSOC) during daytime and nighttime, and to investigate sources and formation pathways of WSOC in individual particle size classes. Mass, WSOC, ${NO_3}^-$, $K^+$, and $Cl^-$ at day and night showed mostly bimodal size distributions, peaking at the size range of $0.32-0.55{\mu}m$(condensation mode) and $3.1-6.2{\mu}m$(coarse mode), respectively, with a predominant condensation mode and a minor coarse mode. While ${NH_4}^+$ and ${SO_4}^{2-}$ showed unimodal size distributions which peaked between 0.32 and $0.55{\mu}m$. WSOC was enriched into nuclei mode particles(< $0.1{\mu}m$) based on the WSOC-to-mass and WSOC-to-water soluble species ratios. The sources and formation mechanisms of WSOC were inferred in reference to the size distribution characteristics of inorganic species(${SO_4}^{2-}$, ${NO_3}^-$, $K^+$, $Ca^{2+}$, $Na^+$, and $Cl^-$) and carbon monoxide. Nuclei mode WSOC was likely associated with primary combustion sources during daytime and nighttime. Among significant sources contributing to the condensation mode WSOC were homogeneous gas-phase oxidation of VOCs, primary combustion emissions, and fresh(or slightly aged) biomass burning aerosols. The droplet mode WSOC could be attributed to aqueous oxidation of VOCs in clouds, cloud-processed biomass burning aerosols, and small contributions from primary combustion sources. From the correlations between WSOC and soil-related particles, and between WSOC and sea-salt particles, it is suggested that the coarse mode WSOC during daytime is likely to condense on the soil-related particles($K^+$ and $Ca^{2+}$), while the WSOC in the coarse fraction during nighttime is likely associated with the sea-salt particles($Na^+$).