• 제목/요약/키워드: primary isolates

검색결과 80건 처리시간 0.027초

초회다제내성 결핵의 위험요인 (Risk Factors for Primary Multidrug Resistant Tuberculosis)

  • 민진홍;박기호;황수희;김진희
    • Tuberculosis and Respiratory Diseases
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    • 제59권6호
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    • pp.600-605
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    • 2005
  • 목 적 : 결핵약으로 치료 받은 적이 없으면서 최소한 아이소니아지드와 리팜핀에 동시에 내성이 있는 결핵균에 감염된 결핵을 초회다제내성 결핵이라 하며 이는 다제내성결핵균에 감염되어 발병한다. 근래에 초회내성결핵은 결핵관리 프로그램의 수행에 있어 중요한 지표로 사용되고 있다. 저자들은 초회다제내성 결핵의 위험요인을 알아내기 위해 환자대조군 연구를 시행하였다. 방 법 : 2001년 1월 1일부터 2003년 6월 30일 동안 국립마산병원에 입원한 29명의 초회다제내성결핵 환자들을 대상으로 환자군을 설정하였고, 대조군은 같은 기간 동안 본원에 입원한 모든 약제에 감성인 결핵환자들을 대상으로 하였다. 초회다제내성 결핵에 대한 의심되는 위험요인들의 교차비를 계산하였다. 결 과 : 다변량로지스틱회귀분석 결과 당뇨병이 초회다제내성 결핵과 통계적으로 유의한 연관성이 있었다(교차비 2.68; 95% 신뢰구간 1.05-6.86). 결 론 : 초회다제내성 결핵의 위험인자로서 당뇨병을 의심할 수 있었으며 향후 추구 연구가 요구된다.

영남지방 돼지의 Bordetella bronchiseptica 감염상황 및 분리균의 생화학적 특성 (Incidence of Bordetella bronchiseptica Infection in Youngnam Swine Herds and the Biochemical Properties of the Organisms Recovered from Pigs with Atrophic Rhinitis)

  • 장희경;김봉환
    • 대한수의학회지
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    • 제28권1호
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    • pp.75-81
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    • 1988
  • The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period from August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) glowing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.

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조선왕조 로부(어가행렬) 의례에 관한 연구 - 형식구조의 파악을 중심으로 - (A Study on the Royal Procession Ceremonies in the Yi Dynasty - Focusing on the Analysis of the Systems -)

  • 백영자
    • 한국의류학회지
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    • 제13권2호
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    • pp.166-179
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    • 1989
  • This study was undertaken to discuss the frameworks of the gorgeous and magnificent royal procession ceremonies in the Yi Dynasty, thereby exploring the classification systems of 'three stages and five segments' on the basis of cultural anthropology and Korean traditional shamanism of ancestral sacrifices. The ceremonies were classified into three phases of (1) cleanup stage, (2) primary objective attendance stage, and (3) completing stage. Among these phases, p. :mary objective attendance stage was further distributed into five segments of (1) road guide, (2) dressing, (3) royal procession, (4) attendants' procession, and (5) back row procession. The isolates and functions of the royal procession ceremonies in the Yi Dynasty were studied through the application of Levi Strauss's structural methodology.

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LAS(Linear Alkybenzene Sulfonate)의 Plasmid에 의한 분해 (Degradation of MS(Linear Alkylbenzene Sulfonate) by Plasmid)

  • 차전옥;유진삼;백형석
    • 한국미생물·생명공학회지
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    • 제22권2호
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    • pp.158-163
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    • 1994
  • Microorganisms capale of utilizing linear alkylbenzene sulfonates(LAS) as sole carbon source were isolated from industrial effluent by using LAS agar plates. The isolated strains were identified as Salmonella sp(BC-2) and Escherichia sp.(BC-3) from the results of morphological, cultural and biochemical tests. The optimal condition for the growth and biodegradation of LAS was the initial pH 7.0 and LAS concentration 0.1%. The isolated BC-2 and BC-3 strains harbored plasmid and LAS-degrading activity was lost when the plasmids were cured by mitomycin C. The plasmids were transformed into E. coli and transformants have the LAS-degrading activity. Isolated strains were examined for primary biodegradation rate of LAS in the medium by methylene blueactive substance(MBAS) method. Of these isolates, BC-2 and BC-3 strains degradated LAS upto 60% and high resistant to CdCl$_{2}$ and HgCl$_{2}$. Isolated strains were sensitive to chloramphenicol, kanamycin, rifampicin, streptomycin and tetracycline but resistant to ampicillin and lincomycin.] Its minimal inhibitory concentration(MIC) for ampicillin was more than 1500 $\mu $g/ml.

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Algal genomics perspective: the pangenome concept beyond traditional molecular phylogeny and taxonomy

  • Lee, JunMo
    • Journal of Species Research
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    • 제10권2호
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    • pp.142-153
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    • 2021
  • Algal genomics approaches provide a massive number of genome/transcriptome sequences and reveal the evolutionary history vis-à-vis primary and serial endosymbiosis events that contributed to the biodiversity of photosynthetic eukaryotes in the eukaryote tree of life. In particular, phylogenomic methods using several hundred or thousands of genes have provided new insights into algal taxonomy and systematics. Using this method, many novel insights into algal species diversity and systematics occurred, leading to taxonomic revisions. In addition, horizontal gene transfers (HGTs) of functional genes have been identified in algal genomes that played essential roles in environmental adaptation and genomic diversification. Finally, algal genomics data can be used to address the pangenome, including core genes shared among all isolates and partially shared strain-specific genes. However, some aspects of the pangenome concept (genome variability of intraspecies level) conflict with population genomics concepts, and the issue is closely related to defining species boundaries using genome variability. This review suggests a desirable future direction to merge algal pangenomics and population genomics beyond traditional molecular phylogeny and taxonomy.

Molecular epidemiology of Toxoplasma gondii in cattle in Korea

  • Kyoo-Tae Kim;Min-Goo Seo
    • Parasites, Hosts and Diseases
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    • 제61권2호
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    • pp.210-215
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    • 2023
  • Toxoplasmosis is a major public health concern, with raw or undercooked meat being the primary source of human infection. Knowledge regarding the molecular epidemiology of Toxoplasma gondii in cattle destined for human consumption in Korea is lacking. The present study aimed to genetically characterize the infectious strains of T. gondii. Overall, 455 cattle blood samples from 84 farms in the Gyeongnam-do (Province) were randomly collected in 2017. Nested PCR analysis revealed that only 3 (0.7%) samples were infected with T. gondii. The B1 gene sequence of T. gondii was observed to be similar (97.3-99.6%) to that of other T. gondii isolates. This is the first study to perform the molecular detection of T. gondii in cattle in Korea. Although the prevalence of infection was low, our findings suggest that cattle present a potential public health issue. It may be crucial to recognize the importance of T. gondii infection in cattle meat.

Reaction of Five Non-cereal Grasses to Five Races and Two Host Selective Toxins of Pyrenophora tritici-repentis

  • Ali, Shaukat;Langham, M.A.C.
    • The Plant Pathology Journal
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    • 제31권3호
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    • pp.245-251
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    • 2015
  • Alternative hosts increase the difficulty of disease management in crops because these alternate hosts provide additional sources of primary inoculum or refuges for diversity in the pathogen gene pool. Agropyron cristatum (crested wheatgrass), Bromus inermis (smooth bromegrass), Pascopyrum smithii (western wheatgrass), Stipa viridula (green needlegrass), and Thinopyrum intermedium (intermediate wheatgrass), commonly identified in range, prairie, verge, and soil reclamation habitats, serve as additional hosts for Pyrenophora tritici-repentis, the cause of tan spot in wheat (Triticum aestivum L.). A. cristatum (five lines), B. inermis (seven lines), P. smithii (four lines), S. viridula (two lines), and T. intermedium (six lines) were tested for their reactions to 30 representative P. tritici-repentis isolates from races 1-5. Plants were grown until the two-three-leaf stage in a greenhouse, inoculated individually with the 30 isolates, held at high humidity for 24 h, and rated after 7 days. All lines developed lesion types 1-2 (resistant) based on a 1-5 rating scale. Also, leaves from an additional plant set were infiltrated with two host selective toxins, Ptr ToxA as a pure preparation and Ptr ToxB as a dilute crude culture filtrate. All lines were insensitive to the toxins. Results indicate that these grass hosts have a limited or nonsignificant role in tan spot epidemiology on wheat in the northern Great Plains. Additionally, the resistant reactions demonstrated by the grass species in this research indicate the presence of resistance genes that can be valuable to wheat breeding programs for improving wheat resistance to P. tritici-repentis.

양송이 마이코곤병(Mycogone perniciosa Magn.)의 항균성 미생물에 관한 연구 (The Antifungal Microorganisms to Mycogone perniciosa Magn. in Cultivated Mushroom, Agaricus bisporus (Lang) Sing)

  • 전창성;차동열;유창현
    • 한국균학회지
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    • 18권2호
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    • pp.96-101
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    • 1990
  • 양송이 주요병해인 마이코곤병(M. perniciosa)의 생물적방제를 위한 항균성 미생물을 선발하기 위하여 실시한 시험 결과를 요약하면 다음과 같다. 1. 미생물의 분리는 Starch casein, Soybean meal, Oat meal 배지에서 균집락 형성과 균집락의 특성이 다른 배지 보다 빠르며, 다양한 미생물의 분리가 용이하였다. 2. 실내시험에서 사상균 91, 세균 342, 방사선균 556균주의 미생물을 분리 선발한 후 Oat meal 배지에서 대치배양하여 항균력이 높은 세균 12, 방사선균 71균주를 선발하였다. 3.실내에서 항균력이 있는 균주에 대해 실시한 1차 포장시험에서 5개 균주가 수량 및 항균력이 우수하였으며, 확인시험에서는 AJ-117, AJ-136, AK-139의3균주가 뚜렷한 항균력이 있었다.

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First Description of Crown Gall Disease on Ginseng

  • Jeon, Yong-Ho;Park, Hoon;Lee, Byeong-Dae;Yu, Yun-Hyun;Chang, Sung-Pae;Kim, Sang-Gyu;Hwang, In-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • 제24권2호
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    • pp.207-210
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    • 2008
  • In March of 2003, tumors (galls) were observed on ginseng seedling roots in ginseng seedbeds at Yeoju, Gyeonggi province, Korea. Symptoms were spherical or galls with about 0.5-1.0cm in diameter formed on the upper through middle parts of the primary roots. Bacterial isolates obtained from the root galls were Gram-negative, rod-shaped with peritrichous flagella, aerobic, not forming yellow or orange colonies on nutrient glucose agar, yeast extract-dextrose $CaCO_3$ agar and nutrient-broth yeast extract agar, non-fluorescent on King's B agar, and non-spore forming, which were identical to characteristics of the genus Agrobacterium. They were identified as Agrobacterium tumefaciens with 0.732-0.993 similarities in 100% probability by the Biolog analyses. The 16S rRNA gene partial sequences of the six isolates tested (Genbank Accession EF486308-EF486313) were 100% homologous to those of other A. tumefaciens strains (GenBank accession AF501343, AY701900, AY701898, AY701899). The above results confirmed that this bacterium is A. tumefaciens. Pathogenicity of the bacteria was proved by the inoculation test on carrot root discs and tomato seedlings. This is the first description of A. tumefaciens causing root gall in ginseng seedling. The disease occurred locally and sparsely, but considering its appearances in seedbeds suggests that the ginseng root gall may become a threat to ginseng in Korea.

Dactynotus 진딧물의 국내 미기록 곤충병원성 곰팡이, Zoophthora phalloides에 관한 보고 (Zoophthora phalloides Batko (Zygomycetes: Entomophthoraceae), a Fungal Parasite of the Aphid Dactynotus species in Korea)

  • 윤철식;성기호;이승환;윤태유;이정운
    • 한국균학회지
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    • 제26권4호통권87호
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    • pp.413-415
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    • 1998
  • 국내 미기록 곤충병원성 곰팡이, Zoophthora phalloides를 1998년 6월경 수원 농업과학기술원 및 전남진흥원내의 망초에 기생하는 진딧물(Dactynotus sp.)에서 발견하였다. 이에 국내에서 처음으로 Z. phalloides를 보고하며, 형태적 특징을 기재한다. 이 균은 소세지 모양의 1차포자를 형성하는 것이 특징이며, 평균길이가 $28.98\;{\mu}m$이었다. 또한 1차포자에서는 두 종류의 2차 분생자병을 형성하였는데, 발아관처럼 짧고 굵거나 긴 막대모양을 띄었고, 각각의 분생자병 끝에서 2차포자가 만들어졌다.

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