• Toxicological Research
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• 제23권4호
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• pp.301-309
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• 2007

Paecilomyces tenuipes (PT), one of the Ascomycetes family, has been used for medicinal purposes due to its broad pharmacological activities. The present study was undertaken to investigate the hepatoprotective effects of PT water extracts against $CCl_4$-induced hepatotoxicity in primary cultures of adult rat hepatocytes. When the extract of PT was directly added into the culture medium at 1, 2, and 5 mg/ml, the extracts not only reduce the $CCl_4$-induced elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase, and lipid peroxide, but also protect cultured hepatocytes from $CCl_4$-induced reduction of reduced glutathione, glutathione reductase, glutathione-S-transferase, glutathione peroxidase, catalase and superoxide dismutase. In addition, the effects of PT water extracts on cytochrome P450 enzymes were relatively marginal, indicating that the hepatoprotective effects of PT extract against $CCl_4$-induced toxicity might not be due to the inhibition of $CCl_4$ activation. In conclusion, the PT extracts were effective in protecting against $CCl_4$ induced hepatotoxicity in hepatocyte cultures, at least in part, by scavenging free radicals, and by modulating enzyme systems involved in cellular oxidative stress.

• 한국양식학회지
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• 제13권1호
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• pp.79-85
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• 2000

자성 및 웅성스테로이드 호르몬들이 Vg 유전자발현에 영향을 미치는지를 미성숙 무지개송어의 배양간세포 막간을 이용하여 조사하였다. 이미 보고된 송어의 Vg gene의 염기배열을 참고로 Vg cDNA 단편(600 bp)을 증폭시킬 수 있는 primer들을 작성하였다. 이들 primer를 이용하여 증폭된 PCR 산물의 염기배열을 결정하여 송어의 Vg cDNA임을 확인하였고, RT-PCR법을 이용하여 배양간세포 그리고 E$_2$ 및 MT 처리된 송어의 간으로부터 Vg mRNA의 전사량 변화를 조사하였다. 호르몬 처리된 간세포 및 송어의 간에서 추출한 total RNA를 이용하여 RT-PCR법으로 분석한 결과 in vivo, in vitro 실험 모두에서 E$_2$ 또는 MT처리된 간세포 및 송어의 간으로부터 Vg mRNA와 Vg 단백질합성이 유도되었고, 이들의 증가 경향은 처리된 호르몬 농도 및 시간에 의존하고 있음이 밝혀졌다. 또한 progesterone, androsterone 그리고 testosterone 등의 웅성호르몬들도 Vg mRNA의 전사를 유도하고 있다는 것이 시사되었다. 이와 같은 결과로부터E$_2$ 뿐 아니라 웅성스테로이드들도 Vg mRNA의 발현을 유도하고 있음이 송어의 in vivo 또는 in vitro 실험에 의해서 확인되었다.

• Fisheries and Aquatic Sciences
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• 제4권4호
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• pp.180-185
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• 2001

Al and Cd-induced inhibition of vitellogenin (VTG) production was examined at the estrogen receptor (ER) level in rainbow trout Oncorhynchus mykiss hepatocytes. The binding of $[^3H]$ $estradiol-17\beta\;(E_2)$ to hepatocytes reached a plateau 3 days after addition of $E_2\;(2\times\;10^{-6} M)$to the medium. The binding activity was linearly reduced with the increased concentrations $(-10^{-5}\;M)$ of 4-hydroxy-tamoxifen (4-OHT) and specific binding linearly increased with the increased doses of $[^3H]\;E_2$, indicating that the radioligand bound to ER. Al $(-10^{-4}\;M)$and Cd $(10^{-6}\;M)$ as well as 4-OHT $(10^{-6}\;M)$ significantly reduced the $[^3H]\;E_2$-binding activity by $30­40\%$, while they completely inhibited VTG production. Al and Cd had no effect on $E_2-human$ $ER\alpha$ binding activity at any concentrations used $(-10^5\;nM\;each)$. These results suggested that Al and Cd inhibited VTG production in part by interfereing with the ER level. Inhibitory effects of these metals on the $E_z-dependent$ upregulation of ER activity are also discussed.

• 한국가축번식학회지
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• 제20권3호
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• pp.259-269
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• 1996

To establish whether or not androgens is responsible for the induction of vitellogenin(Vg) synthesis and secretion, primary hepatocytes prepared from immature eels were used. The results are follows: 1. Eel hepatocytes were prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in medium for 10 days with minimal cell loss. 2. Estradiol-17$\beta$(E2) alone was insufficient to induce Vg synthesis. The combination of E2 with methyltestosterone(MT) markedly stimulated Vg synthesis. High vg production occurred in MT concentration from 10-6~10-5M in the presence of E2 (10-6M). Testosterone and androsterone were also effective, but progesterone was not effective in inducing Vg synthesis. Neither MT alone nor testosterone and androsterone alone had any effect on Vg synthesis. 3. E2-primed hepatocytes showed Vg synthesis in both media with and without hormones 1 day after culture. In the cultures with the vehicle, MT, or progesterone, the rate of synthesis seemed to decrease with time. But the combination of E2 and MT showed an intense increase in Vg synthesis. Hepatocytes isolated from E2-primed eels also required androgens for continuating of Vg synthesis. 4. These results demonstrate that androgens act together with E2 in synthesis and secretion of eel Vg.

• 한국식품영양과학회지
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• 제33권8호
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• pp.1246-1251
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• 2004

본 실험은 사염화탄소 독성에 대한 몇 가지의 식물소재들- 솔잎, 콩나물, 인진쑥, 표고버섯 - 의 간보호 효과를 조사하기 위하여 수행되었다. 우선적으로 일차 간세포 배양실험에서 식물추출물(열수 또는 에탄올)을 첨가(20 mg/mL 배지)하고 2시간 후에 사염화탄소를 처리(50$\mu$L)한 결과, 배지내의 AST와 ALT 효소활성은 다음과 같았다. 솔잎은 에탄올 및 열수 추출물(P<0.05)에서, 표고버섯은 열수 추출물(p<0.01∼0.05)에서, 그리고 콩나물과 인진쑥은 추출방법에 따라 유의성 있는(p<0.01) 감소를 보였다. 또한, 3주간의 식물 추출물을 첨가한 사료를 사전급여하고 사염화탄소를 2일간 복강주사(1 mg/kg 체중)한 동물실험에서 혈청내의 AST 및 ALT 활성은 간세포 배양의 결과와 유사하게 솔잎과 콩나물에서 낮은 활성효과(p<0.01)를 보였다. 정상군에 대한 사염화탄소의 투여는 지질과산화물과 cytochrome P-450의 함량, 그리고 xanthine oxidase(XOD) 활성을 크게 증가시켰다. 그러나 식물소재 중에서 솔잎, 콩나물, 인진쑥 등을 추출물을 첨가한 실험군에 대한 사염화탄소 처리는 일반적으로 지질과 산화와 XOD 활성증가를 억제하는 것(p<0.01)으로 나타난 반면에, cytochrome P-450의 함량 증가는 솔잎과 인진쑥의 첨가군에서 관찰되었다. 따라서 본 실험에서 cytochrome P-430의 함량증가에 대한 식물 추출물들의 효과는 확실하지 않았지만, 솔잎과 콩나물의 추출물은 항산화관련 실험에서 대부분 탁월한 효과를 나타내었다.

• 생약학회지
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• 제37권4호
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• pp.294-301
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• 2006

The aim of this study was to investigate the protective activity of gomisin A and gomisin N, bioactive lignan components isolated from Schizandae Fructus, on hepatocyte injury induced by carbon tetrachloride($CCl_4$, 10 mM), t-butyl hydroperoxide(TBH, 0.5 mM), and D-galactosamine(GalN, 30 mM). Primary cultures of rat hepatocyte(18 h culture) were treated with $CCl_4$, TBH or GalN and various concentrations(0.1, 1, 10, $100{\mu}M$) of gomisin A or gomisin N. $CCl_4$ significantly increased the levels of lactate dehydrogenase(LDH), alanine aminotransferase(ALT), and aspartate aminotransferase(AST). These increases were inhibited by gomisin N. TBH significantly increased the level of AST; an increase that was inhibited by gomisin N. GalN markedly increased the levels of LDH and ALT, and these increases was significantly inhibited by both gomisin A and gomisin N. These results suggest that gomisin A and gomisin N have the hepatoprotective activity.

• 생약학회지
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• 제38권2호통권149호
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• pp.139-147
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• 2007

The aim of this study was to investigate the protective activity of curcumin, demethoxycurcumin and bisdemethoxycurcumin, isolated from Curcuma longa Linne, on hepatocyte injury induced by carbon tetrachloride (CCl$_4$,10 mM), t-butyl hydroperoxide (TBH, 0.5 mM) and D-galactosamine (GaIN,30 mM). Primary cultures of rat hepatocyte (18 h culture) were treated with CCl$_4$, TBH or GaIN and various concentrations (0.1, 1, 10 and 100 ${\mu}$M) of curcumin, demethoxycurcumin and bisdemethoxycurcumin CCl$_4$ significantly increased the levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The increases in LDH, ALT and AST levels were inhibited by curcumin. Demethoxycurcumin and bisdemethoxycurcumin decreased the levels of AST. Curcumin inhibited the increases in ALT and AST levels induced by TBH. The increased levels of LDH, ALT and AST induced by TBH were inhibited by bisdemethoxycurcumin. GaIN markedly increased the levels of LDH, ALT and AST. These increases were significantly inhibited by bisdemethoxycurcumin. The increase in AST level was inhibited by curcumin. These results suggest that curcumin and bisdemethoxycurcumin have potent hepatoprotective activities.

• 한국수산과학회지
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• 제39권1호
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• pp.23-26
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• 2006

Effects of sera from several fish species and insulin on the development of cultured Silurus asotus hepatocytes were investigated. Hepatocytes with high viability (95%) were obtained from the livers of male catfish by two step collagenase perfusion. Isolated hepatocytes, initially showed a typical round-shape, firmly attached to the culture dish within 24 h. In the presence of catfish serum, hepatocytes attached each other, spread well on the dish and developed into monolayer after 3-4 days of incubation. Cells within the established monolayer became polygonal in shape and their nuclei and boundaries being clearly visible under the microscope. In contrast, when incubated in FBS-supplemented or serum-free medium, cells managed to form small clusters, each made of 2-10 cells. Cells in FBS-supplemented medium further developed into larger clusters. However, these clusters failed to develope into monolayer. In addition, when insulin was deprived from culture medium, formation of monolayer also failed. From these data, it can be concluded that the presence of both catfish serum and insulin is necessary for the formation of monolayer of catfish hepatocytes and the functional role of fish serum may differ from that of insulin and can not be displaced by FBS-supplementation.

• 한국간담췌외과학회지
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• 제27권4호
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• pp.342-349
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• 2023

Backgrounds/Aims: Liver organoids have emerged as a powerful tool for studying liver biology and disease and for developing new therapies and regenerative medicine approaches. For organoid culture, Matrigel, a type of extracellular matrix, is the most commonly used material. However, Matrigel cannot be used for clinical applications due to the presence of unknown proteins that can cause immune rejection, batch-to-batch variability, and angiogenesis. Methods: To obtain human primary hepatocytes (hPHs), we performed 2 steps collagenase liver perfusion protocol. We treated three small molecules cocktails (A83-01, CHIR99021, and HGF) for reprogramming the hPHs into human chemically derived hepatic progenitors (hCdHs) and used hCdHs to generate liver organoids. Results: In this study, we report the generation of liver organoids in a collagen scaffold using hCdHs. In comparison with adult liver (or primary hepatocyte)-derived organoids with collagen scaffold (hALO_C), hCdH-derived organoids in a collagen scaffold (hCdHO_C) showed a 10-fold increase in organoid generation efficiency with higher expression of liver- or liver progenitor-specific markers. Moreover, we demonstrated that hCdHO_C could differentiate into hepatic organoids (hCdHO_C_DM), indicating the potential of these organoids as a platform for drug screening. Conclusions: Overall, our study highlights the potential of hCdHO_C as a tool for liver research and presents a new approach for generating liver organoids using hCdHs with a collagen scaffold.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.305-306
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• 2003

To treat fulminant hepatic failure (FHF) patients, various extracorporeal bioartificial liver (BAL) systems have been developed. Several requirements should be met for the development of BAL systems: hepatocytes should be cultured in a sufficiently high density; their metabolic functions should be of a sufficiently high level and duration; and the BAL systems module should permit scaling-up and aseptic handling. Several investigators have found that freshly isolated primary hepatocytes can be cultured into three dimensional, tightly packed, freely suspended, multicellular aggregates, or spheroids. These specialized cell structures exhibited enhanced liver specific functions and a prolonged differentiated state compared to cells maintained in a monolayer culture. Cells in spheroids appear to mimic the morphology and ultrastructure of the in vivo liver lobule. The ability of hepatocytes to organize into three-dimensional structures was hypothesized to contribute to their enhanced liver-specific activities. In this study, the ammonia removal rate and urea secretion rate of pig hepatocytes spheroids encapsulated in Ca-alginate bead were determined. A packed-bed bioreactor with encapsulated pig hepatocytes was devised as BAL support system. The efficacy of the system was evaluated in vitro.