Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effect of Fish Serum on the Primary Monolayer Culture of Catfish (Silurus asotus) Hepatocytes

어류혈청이 메기(Silurus asotus) 간세포의 단층배양에 미치는 영향

  • Kwon, Hyuk-Chu (Department of Applied Biological Sciences, Sunmoon University) ;
  • Choi, Seong-Hee (Department of Applied Biological Sciences, Sunmoon University) ;
  • Kim, Eun-Hee (Department of Applied Biological Sciences, Sunmoon University) ;
  • Han, Deug-Woo (Department of Applied Biological Sciences, Sunmoon University) ;
  • Kwon, Joon-Yeong (Department of Applied Biological Sciences, Sunmoon University)
  • 권혁추 (선문대학교 응용생물과학부) ;
  • 최성희 (선문대학교 응용생물과학부) ;
  • 김은희 (선문대학교 응용생물과학부) ;
  • 한덕우 (선문대학교 응용생물과학부) ;
  • 권준영 (선문대학교 응용생물과학부)
  • Published : 2006.02.01
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Abstract

Effects of sera from several fish species and insulin on the development of cultured Silurus asotus hepatocytes were investigated. Hepatocytes with high viability (95%) were obtained from the livers of male catfish by two step collagenase perfusion. Isolated hepatocytes, initially showed a typical round-shape, firmly attached to the culture dish within 24 h. In the presence of catfish serum, hepatocytes attached each other, spread well on the dish and developed into monolayer after 3-4 days of incubation. Cells within the established monolayer became polygonal in shape and their nuclei and boundaries being clearly visible under the microscope. In contrast, when incubated in FBS-supplemented or serum-free medium, cells managed to form small clusters, each made of 2-10 cells. Cells in FBS-supplemented medium further developed into larger clusters. However, these clusters failed to develope into monolayer. In addition, when insulin was deprived from culture medium, formation of monolayer also failed. From these data, it can be concluded that the presence of both catfish serum and insulin is necessary for the formation of monolayer of catfish hepatocytes and the functional role of fish serum may differ from that of insulin and can not be displaced by FBS-supplementation.

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References

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