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Hepatoprotective Activities of Gomisin A and Gomisin N  

Heo Jeong-Haing (College of Pharmacy, Sungkyunkwan University)
Park Jin-Gu (College of Pharmacy, Sungkyunkwan University)
Cheon Ho-Jun (College of Pharmacy, Sungkyunkwan University)
Kim Yeong-Shik (College of Pharmacy, Seoul National University)
Kang Sam-Sik (College of Pharmacy, Seoul National University)
Hung Tran Manh (College of Pharmacy, Chungnam National University)
Bae Ki-Hwan (College of Pharmacy, Chungnam National University)
Lee Sun-Mee (College of Pharmacy, Sungkyunkwan University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.4, 2006 , pp. 294-301 More about this Journal
Abstract
The aim of this study was to investigate the protective activity of gomisin A and gomisin N, bioactive lignan components isolated from Schizandae Fructus, on hepatocyte injury induced by carbon tetrachloride($CCl_4$, 10 mM), t-butyl hydroperoxide(TBH, 0.5 mM), and D-galactosamine(GalN, 30 mM). Primary cultures of rat hepatocyte(18 h culture) were treated with $CCl_4$, TBH or GalN and various concentrations(0.1, 1, 10, $100{\mu}M$) of gomisin A or gomisin N. $CCl_4$ significantly increased the levels of lactate dehydrogenase(LDH), alanine aminotransferase(ALT), and aspartate aminotransferase(AST). These increases were inhibited by gomisin N. TBH significantly increased the level of AST; an increase that was inhibited by gomisin N. GalN markedly increased the levels of LDH and ALT, and these increases was significantly inhibited by both gomisin A and gomisin N. These results suggest that gomisin A and gomisin N have the hepatoprotective activity.
Keywords
gomisin A; gomisin N; Schizandrae Fructus; hepatoprotective activity;
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