• 대한통합의학회지
• /
• 제8권3호
• /
• pp.53-62
• /
• 2020

Purpose :Periodontal ligament stem cells maintain tissue homeostasis in periodontal ligament. The purpose of this study was to determine the characteristics of periodontal ligament stem cells isolated from premolar teeth and observe protective effects against oxidative damage caused by Triethylene glycol dimethacrylate (TEGDMA) following treatment with N-acetylsysteine amide (NACA) drug known as enzymatic antioxidants. Methods : Primary periodontal ligament stem cell (PDSC) culture was performed from simply extracted human premolar of orthodontic patients. The characteristics of the primary cultured PDSCs was analyzed using the FACS system. PDSCs was incubated with TEGDMA and NACA. The cell proliferation and survival was determined using WST-1 assay. Collected data were analyzed using SPSS Window 20. Results : Primary cultured PDSCs grow on the floor and develop rapidly in a cluster form from up to 14 days. The morphology of PDSCs showed the spindle-shaped cells and grew directionally. FACS analysis, In addition, positive expression of visible cells were observed in mesenchymal stem cell biomarkers. PDLSCs cell viability was significantly decreased at high concentration in both 3 and 6 hours after TEGDMA treatment. We observed a decrease in the number of cells as well as a morphological change of PDLSCs. Antioxidative effect was notable since the death of PDLSC death was significantly inhibited compared to the control group at 24 and 48 hours after NACA treatment. Conclusion : Therefore, based on the results of this study, further research should be encouraged considering the development of clinical treatment methods using various antioxidants as well as regenerative engineering techniques utilizing periodontal ligament stem cells.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권1호
• /
• pp.35-42
• /
• 2015

In cardiovascular disorders, understanding of endothelial cell (EC) function is essential to elucidate the disease mechanism. Although the mouse model has many advantages for in vivo and in vitro research, efficient procedures for the isolation and propagation of primary mouse EC have been problematic. We describe a high yield process for isolation and in vitro culture of primary EC from mouse arteries (aorta, braches of superior mesenteric artery, and cerebral arteries from the circle of Willis). Mouse arteries were carefully dissected without damage under a light microscope, and small pieces of the vessels were transferred on/in a Matrigel matrix enriched with endothelial growth supplement. Primary cells that proliferated in Matrigel were propagated in advanced DMEM with fetal calf serum or platelet-derived serum, EC growth supplement, and heparin. To improve the purity of the cell culture, we applied shearing stress and anti-fibroblast antibody. EC were characterized by a monolayer cobble stone appearance, positive staining with acetylated low density lipoprotein labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate, RT-PCR using primers for von-Willebrand factor, and determination of the protein level endothelial nitric oxide synthase. Our simple, efficient method would facilitate in vitro functional investigations of EC from mouse vessels.

• Nuclear Engineering and Technology
• /
• 제47권7호
• /
• pp.895-906
• /
• 2015

Background: Mitigation of primary water stress corrosion cracking (PWSCC) is a significant issue in the nuclear industry. Advanced nickel-based alloys with lower susceptibility have been adopted, although they do not seem to be entirely immune from PWSCC during normal operation. With regard to structural integrity assessments of the relevant components, an accurate evaluation of crack growth rate (CGR) is important. Methods: For the present study, the extended finite element method was adopted from among diverse meshless methods because of its advantages in arbitrary crack analysis. A user-subroutine based on the strain rate damage model was developed and incorporated into the crack growth evaluation. Results: The proposed method was verified by using the well-known Alloy 600 material with a reference CGR curve. The analyzed CGR curve of the alternative Alloy 690 material was then newly estimated by applying the proven method over a practical range of stress intensity factors. Conclusion: Reliable CGR curves were obtained without complex environmental facilities or a high degree of experimental effort. The proposed method may be used to assess the PWSCC resistance of nuclear components subjected to high residual stresses such as those resulting from dissimilar metal welding parts.

• 대한두개안면성형외과학회지
• /
• 제25권1호
• /
• pp.1-10
• /
• 2024

The facial nerve stimulates the muscles of facial expression and the parasympathetic nerves of the face. Consequently, facial nerve paralysis can lead to facial asymmetry, deformation, and functional impairment. Facial nerve palsy is most commonly idiopathic, as with Bell palsy, but it can also result from a tumor or trauma. In this article, we discuss traumatic facial nerve injury. To identify the cause of the injury, it is important to first determine its location. The location and extent of the damage inform the treatment method, with options including primary repair, nerve graft, cross-face nerve graft, nerve crossover, and muscle transfer. Intracranial proximal facial nerve injuries present a challenge to surgical approaches due to the complexity of the temporal bone. Surgical intervention in these cases requires a collaborative approach between neurosurgery and otolaryngology, and nerve repair or grafting is difficult. This article describes the treatment of peripheral facial nerve injury. Primary repair generally offers the best prognosis. If primary repair is not feasible within 6 months of injury, nerve grafting should be attempted, and if more than 12 months have elapsed, functional muscle transfer should be performed. If the affected nerve cannot be utilized at that time, the contralateral facial nerve, ipsilateral masseter nerve, or hypoglossal nerve can serve as the donor nerve. Other accompanying symptoms, such as lagophthalmos or midface ptosis, must also be considered for the successful treatment of facial nerve injury.

• 화약ㆍ발파
• /
• 제38권1호
• /
• pp.14-22
• /
• 2020

천공 중 비트의 손상은 천공 효율을 떨어뜨리는 주요 원인이다. 현재 천공 중 비트의 마모와 손상에 대한 관리는 현장 작업자의 기술에 의지하고 있어 이를 예측하고 관리하는 시스템의 구축이 시급하다. 비트의 정확한 손상 시점을 판단하기 위해 천공 중 발생하는 소음 자료를 이용해 웨이블릿 변환 분석을 하였다. 이를 통해 얻은 시간-주파수 스펙트럼을 분석한 결과 천공 후 652.8 sec에 주파수 500 Hz 구간에서 웨이블릿 변환 값이 0.9로 최대치가 되는 이상점을 확인하였다. 또한, 천공 속도 분석을 통해 이상치가 발견된 후 천공 속도가 평균인 32 mm/s 보다 9 mm/s 줄어든 것을 확인하였다. 이러한 결과를 통해 웨이블릿 변환 분석을 이용해 천공 중 드릴 비트의 손상을 진단할 수 있음을 보였다.

• ETRI Journal
• /
• 제31권5호
• /
• pp.604-606
• /
• 2009

The primary dose effects on an insulated gate bipolar transistor (IGBT) irradiated with a $^{60}Co$ gamma-ray source are found in both of the components of the threshold shifting due to oxide charge trapping in the MOS and the reduction of current gain in the bipolar transistor. In this letter, the IGBT macro-model incorporating irradiation is implemented, and the electrical characteristics are analyzed by SPICE simulation and experiments. In addition, the collector current characteristics as a function of gate emitter voltage, VGE, are compared with the model considering the radiation damage of different doses under positive biases.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 2004년도 춘계 학술발표회 제16권1호
• /
• pp.412-415
• /
• 2004

This study was performed to verify the behaviors of fiber Bragg grating (FBG) sensors attached to the containment structure in the nuclear power plant as a part of structural integrity test which demonstrates that the structural response of the non-prototype primary containment structure is within predicted limits plus tolerances when pressurized to $115\%$ of containment design pressure, and that the containment does not sustain any structural damage.

• Toxicological Research
• /
• 제7권2호
• /
• pp.231-238
• /
• 1991

The pesticide and carcinogen ethylene dibromide(EDB) is metabolized both by cytosolic GSH S-transferase and by microsomal mixed function oxygenase. Cytochrome P-450 IIE1 appears to be major enzyme to metabolize EDB.EDB is activated to a mutagen by enzymatic conjugation with glutathione (GSH). Such activation is an exception to the general mode of detoxification via GSH S-transferase action. The primary DNA adduct (>95) is S-[2-(N7-guanyl)ethyl] GSH and a minor adduct is S-[2-(N7-guanyl)ethyl]cysteine, which is excreted in the urine and may serve as a biomarker of damage.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.97-98
• /
• 2001

Alpha-tocopherol (alpha-Toc) is a classical lipophilic antioxidant well known as a scavenger of free radicals in a hydrophobic milieu. The primary function of alpha-Toc is to stabilize cellular and subcellar membrane by preventing peroxidative damage of structural polyunsaturated fatty acids (PUFA). The characteristic aroma of sweet smelt Precoglossun altivelis is known as oxida breakdown products of PUFA ironically. (omitted)

• 한국지진공학회논문집
• /
• 제15권3호
• /
• pp.37-43
• /
• 2011

구조물 내 설치된 파이프시스템은 주로 내부구성원들의 인간생활에 근간이 되는 기반시설로서 현대 도시생활의 생명선과 같은 역할을 한다. 이들 구조물 내 파이프시스템이 만약 지진발생에 의하여 손상될 경우 1차적으로 구조물 내 기능성이 저하되고 구조물 내 많은 정신적, 물질적 피해가 발생할 수 있다. 실제 내진공학에서 지진발생에 따른 비구조재요소의 거동은 크게 중요하게 고려되지 않으나 인적 및 물적 피해와 매우 밀접하게 연관되는 가스 혹은 수계파이프시스템에 대한 비구조적 요소의 거동예측 및 성능평가 연구는 보다 효과적인 구조물 유지관리를 위하여 그 필요성이 크다고 판단된다. 본 연구는 현재 일반적으로 널리 시공되어져 있는 노후 철근콘크리트 빌딩구조물 내 설치된 가스 혹은 수계파이프시스템에 대하여 실제 지진발생이 예측되는 거동을 살펴보고 이들 거동에 따른 성능평가 및 현 설계기준에 대한 검토도 병행하여 수행하고자 한다. 이를 위하여 본 연구에서는 해석적으로 발생 가능한 총 10회의 지진파에 대하여 현재 실제 건물 내 기설치된 수계파이프시스템 모델링 및 해석을 통하여 그 결과를 검증, 평가하였으며 부가적으로 실제 발생 가능한 파괴유형 분석을 통하여 현 설치된 수계파이프시스템의 설계에 대한 적절한 내진보강방법에 대하여도 제안하고자 한다.