• 대한의생명과학회지
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• 제23권3호
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• pp.251-260
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• 2017

Platelet activation is essential at the sites of vascular injury, which leads to hemostasis through adhesion, aggregation, and secretion process. However, potent and continuous platelet activation may be an important reason of circulatory disorders. Therefore, proper regulation of platelet activation may be an effective treatment for vascular diseases. In this research, inhibitory effects of cordycepin (3'-deoxyadenosine) on platelet activation were determined. As the results, cordycepin increased cAMP and cGMP, which are intracellular $Ca^{2+}$-antagonists. In addition, cordycepin reduced collagen-elevated $[Ca^{2+}]_i$ mobilization, which was increased by a cAMP-dependent protein kinase (PKA) inhibitor (Rp-8-Br-cAMPS), but not a cGMP-protein kinase (PKG) inhibitor (Rp-8-Br-cGMPS). Furthermore, cordycepin increased $IP_3RI$ ($Ser^{1756}$) phosphorylation, indicating inhibition of $IP_3$-mediated $Ca^{2+}$ release from internal store via the $IP_3RI$, which was strongly inhibited by Rp-8-Br-cAMPS, but was not so much inhibited by Rp-8-Br-cGMPS. These results suggest that the reduction of $[Ca^{2+}]_i$ mobilization is caused by the cAMP/A-kinase-dependent $IP_3RI$ ($Ser^{1756}$) phosphorylation. In addition, cordycepin increased the phosphorylation of VASP ($Ser^{157}$) known as PKA substrate, but not VASP ($Ser^{239}$) known as PKG substrate. Cordycepin-induced VASP ($Ser^{157}$) phosphorylation was inhibited by Rp-8-Br-cAMPS, but was not inhibited by Rp-8-Br-cGMPS, and cordycepin inhibited collagen-induced fibrinogen binding to ${\alpha}IIb/{\beta}_3$, which was increased by Rp-8-Br-cAMPS, but was not inhibited by Rp-8-Br-cGMPS. These results suggest that the inhibition of ${\alpha}IIb/{\beta}_3$ activation is caused by the cAMP/A-kinase-dependent VASP ($Ser^{157}$) phosphorylation. In conclusion, these results demonstrate that inhibitory effects of cordycepin on platelet activation were due to inhibition of $[Ca^{2+}]_i$ mobilization through cAMP-dependent $IP_3RI$ ($Ser^{1756}$) phosphorylation and suppression of ${\alpha}IIb/{\beta}_3$ activation through cAMP-dependent VASP ($Ser^{157}$) phosphorylation. These results strongly indicated that cordycepin might have therapeutic or preventive potential for platelet activation-mediated disorders including thrombosis, atherosclerosis, myocardial infarction, or cardiovascular disease.

• 생명과학회지
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• 제18권9호
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• pp.1207-1211
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• 2008

Pro-inflammatory cytokine인 종양괴사인자-$\alpha$ (TNF-$\alpha$)는 B형 간염바이러스(HBV) 감염에 대한 면역반응의 중요한 매개인자이다. TNF-$\alpha$의 생산은 전사수준에서 주로 조절되며, TNF-$\alpha$ promoter 다형성은 TNF-$\alpha$ 유전자 발현을 변화시키기 때문에, TNF-$\alpha$ promoter 다형성이 만성 HBV 감염의 병태생리에 영향을 미칠 가능성이 높다. 그러므로, 본 연구에서는 TNF-$\alpha$ promoter 다형성과 만성 HBV 감염의 연관성을 조사하였다. 본 연구는 만성 HBV 감염환자 181명, HBV 감염이 자연적으로 치유된 201명, 그리고 건강한 대조군 170명을 대상으로 하였다. TNF-$\alpha$ promoter의 -308G/A와 -238G/A 다형성은 PCR-RFLP법을 통하여 분석하였다. 분석결과, 환자군에서 -308과 -238 유전자형의 분포는 자연치유군 및 건강대조군에서의 분포와 통계적인 차이를 보이지 않았다(p>0.05). 또한, 그룹 간에 allele frequency도 통계적 차이가 없었다(p>0.05). 따라서, 본 연구의 결과는 한국인에서 TNF-$\alpha$ -308과 -238 promoter 다형성은 만성 B형 간염 바이러스 감염의 발생과 연관성이 없음을 시사한다.

• 경영정보학연구
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• 제14권2호
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• pp.1-19
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• 2012

최근 의료서비스 추세는 소비자의 교육과 생활수준의 향상으로 인해 의료기관이 추구하는 일방적인 형태의 의료서비스에서 벗어나 보다 차별화되고 지속적으로 자신의 건강관리가 가능한 능동형 의료서비스 형태로 변화하고 있다. 따라서 스마트 텔레메디슨 시스템은 기존의 단순한 혈압, 혈당, 심장박동, 체온 측정기 등에 블루투스와 이동통신 모듈의 결합을 통해서 의료서비스를 제공하며, 환자가 인식하지 못하는 사이에 환자의 건강 상태 패턴을 분석하여 예방적인 건강관리가 가능한 능동형 유비쿼터스 의료서비스를 제공하게 한다. 즉 온라인과 오프라인의 의료서비스 환경이 상호 연결되어 시간과 장소에 구애 받지 않고 스마트폰과 모바일 의료검진 디바이스를 통해서 양방향으로 의료서비스를 제공한다. 특히 정보통신 기술의 발전과 더불어 새롭게 등장하는 스마트 텔레메디슨 서비스 사업자를 통해 환자들이 효과적인 의료 검진을 위해 장시간 이동하고 대기하는 시간과 비용을 절감하고 환자의 종적 건강 데이터관리를 통해서 환자 스스로가 건강관리를 하게 한다. 또한 개인화된 맞춤형 예방 건강관리 의료서비스를 이용하게 함으로써 다양한 형태의 의료서비스 비즈니스 상거래를 가능하게 하고 있다. 본 연구는 예방의학적인 스마트 텔레메디슨 시스템을 정의하고 이를 기반으로 한 비즈니스 모델 설계와 분석 및 평가모델을 제시하고자 한다.

• 한국산업보건학회지
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• 제17권3호
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• pp.192-202
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• 2007

Objectives: The objectives of this research are to examine the missions, organization, and programs of the environmental, health and safety(EHS) offices of the major research universities in the U.S.A., particularly the case of Massachusetts Institute of Technology(MIT) EHS Office, and to suggest ways to address the EHS issues of the universities and research institutes in South Korea. Methods: The top 30 research universities in the U.S.A. were selected by the total amounts of research funds they annually received. The web sites of the 30 universities were searched to identify the names of the departments that address environment, health, and/or safety related issues, the missions of those departments, the major programs of those departments, and the number of staff in those departments. Also, a case study was conducted for the Environment Health and Safety Management System and the Environment, Health and Safety Office of the MIT, using literature and web searches and a meeting with the Director of the MIT Environment, Health and Safety Office. Results: All the top 30 research universities in the U.S.A. had designated departments that dealt with EHS issues. Most of them were by the name of or similar to environmental, health and safety. The mission statements of those departments were mostly about improving the safety and health of their community members, improving the environment, and complying to EHS regulations. Most of those departments had an environmental management program, industrial hygiene program, radiation protection program, safety program, and biosafety program as their major programs. The components of the environment, health and safety management system(EHS-MS) of the MIT were examined in detail. In contrast, not many universities in South Korea had designated departments that dealt specifically with EHS issues. Also, the number of fulltime staff for EHS was only 1-2 in most of the Korean universities and their work duties included only general safety, while neglecting other health/environment related issues. Conclusions: Well organized and functioning environmental, health and safety offices were present in all of the top 30 research universities in the U.S.A., whereas similar organizations of the universities in South Korea were virtually non-existent and/or had very limited EHS activities. Therefore, in order to reduce potential risks of accidents and health problems in the Korean universities and research institutions, well established and functioning EHS-MSs and EHS offices are warranted. The case of the EHS-MS and EHS Office in the MIT demonstrate a successful case to follow.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.481-489
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• 2017

Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015. Cattle that grazed in mountainous areas showed a greater prevalence of tick-borne infections with an increased Theileria orientalis infection rate (54.7%) compared to that in non-grazing cattle (16.3%) (P<0.001). Accordingly, the red blood cell (RBC) count and hematocrit (HCT) values of grazing cattle were significantly lower than those of non-grazing cattle throughout the season (P<0.05). Moreover, RBC, hemoglobin (Hb), and HCT of T. orientalis-positive group were significantly lower than those of T. orientalis-negative group (P<0.05). T. orientalis is a widespread tick-borne pathogen in Korea. Grazing of cattle in mountainous areas is closely associated with an increase in T. orientalis infection (RR=3.4, P<0.001), and with consequent decreases in RBC count and HCT. Thus, these findings suggest that the Hanwoo cattle in mountainous areas of Korea are at a high risk of infection by T. orientalis, which can lead to hematological alterations. This study highlights the necessity of preventive strategies that target T. orientalis infection.

• 한국사회복지학
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• 제39권
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• pp.103-127
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• 1999

이 논문은 청소년의 음주수준과 부모의 통제 간의 관계와 학년에 따른 두 변수간의 관계를 밝히는 것을 주목적으로 하고 있다. 청소년의 음주수준은 음주빈도에 따라 금주, 호기심 수준, 실험적 수준, 습관적 수준으로 구분하였으며, 부모의 통제에는 부모의 감독, 자녀의 행동에 대한부모의 규칙 유무, 청소년의 음주에 대한부모의 태도로 측정되었다. 연구결과, 학년 변수의 영향을 고려하지 않았을 때 고등학생의 음주 수준과 부모의 통제와의 관계는 대부분 통계적으로 유의미한 것으로 밝혀졌는데, 부모의 감독수준이 높을수록 그리고 청소년 자녀의 음주를 허용하지 않는 태도를 가지고 있는 부모를 둔 학생일수록 금주하는 비율이 높았다. 학년 변수의 영향을 고려했을 때 고등학생의 음주수준과 부모의 감독수준 간의 관계는 학년이 높아질수록 점차 약화되고 있었으나 통계적으로 유의미하여 선행 연구결과와 일치하는 것으로 나타났다. 고등학생의 행동에 대한 부모의 규칙과 음주수준 간의 관계는 2학년에서 그리고 고등학생 자녀의 음주에 대한 부모의 허용여부와 음주수준 간의 관계는 2학년과 3학년에서 유의미한 것으로 나타났다. 또한 이 연구조사에 참여한 학생들은 저학년에서 고학년으로 올라가면서 부모의 감독수준은 낮아지고, 자신의 행동에 대해 부모가 규칙을 가지고 있지 않다고 응답하는 비율이 증가하고 있으며, 부모가 자신의 음주를 허용하거나 부모의 태도가 불분명한 정도가 높아진다고 응답하였다. 이 논문의 장점 중의 하나는 예방프로그램의 핵심인 이론적 토대를 제공하였다는 점이다. 예방프로그램은 수정이 가능한 요인들에 초점을 두고 요인들의 변화를 유도해야 한다. 부모-자녀 간의 관계 증진을 목표로 하는 개입은 사회복지사들에게 매우 익숙하기 때문에 사회복지사는 청소년 음주 및 약물남용 예방프로그램의 일환으로 부모가 미성년 자녀의 음주에 대한 명확한 메시지를 자녀에게 전달하고 자녀의 행동에 대한 규칙을 세우며 부모와 자녀간의 원활한 의사소통을 증진시킬 수 있는 효과적인 양육방식과 관련된 기술을 습득할 수 있도록 원조해야 할 것이다.

• 한국환경보건학회지
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• 제38권6호
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• pp.541-549
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• 2012

Objectives: This study was undertaken in order to evaluate a potential mechanism involved in gastro-intestinal problems observed in autistic subjects and uses an animal model of autism investigation. Methods: BTBR T+tf/J, a mouse strain with typical socio-behavioral characteristics of autistic subjects and FVB mice with highly social behaviors as the control strain were used. Both genders of mice aged three weeks and six months were used from four separate litters for each strain. Serum was prepared following cardiac puncture, and mesenteric lymph nodes were collected for in vitro stimulation and enumeration of major immune cell proportion. Results: The level of serum IgA was significantly enhanced in six-month-old BTBR mice compared with three-week-old BTBR, which was not observed with the FVB control mice. The serum IgE level was also higher among BTBR mice than among age-sex matched FVB mice, respectively. Considering the ratio of interleukin-4 vs interferon-gamma production from mesenteric lymph node T cells, skewedness toward type-2 reactivities was observed. In addition, the proportion of B cells in mesenteric lymph nodes was significantly higher in BTBR mice than in FVB mice. Conclusion: Upregulation of mucosal immunity related with enhanced type-2 immune reactivity observed in BTBR mice could be involved with the etiology of gastro-intestinal abnormalities in autism.

• Asian Pacific Journal of Cancer Prevention
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• 제15권13호
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• pp.5195-5200
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• 2014

Background: The purpose of this study was to analyze our series of liver resections for metastatic colorectal carcinoma (mCRC) to determine prognostic factors affecting survival and to evaluate the potential roles of neoadjuvant or adjuvant chemotherapy. Materials and Methods: Ninety-nine patients who underwent metastasectomy for liver metastases due to colorectal cancer at the Department of Medical Oncology, 9 Eylul University Hospital between 1996 and 2010 were evaluated in this study. The patients were followed through July 2013. Demographic, perioperative, laboratory, radiological and chemotherapy as well as survival data were obtained by retrospective chart review. Results: In 47 (47.5%) patients, liver metastases were unresectable at initial evaluation; the remaining 52 (52.5%) patients exhibited resectable liver metastases. Simultaneous hepatic resection was applied to 52 (35.4%) patients with synchronous metastasis, whereas 5 (64.5%) patients underwent hepatic resection after neoadjuvant chemotherapy. Forty-two patients with metachronous metastasis underwent hepatic resection following neoadjuvant chemotherapy. R0 resection was obtained in 79 (79.8%) patients. A second hepatectomy was performed in 22 (23.2%) patients. Adjuvant chemotherapy was given to 85 (85.9%) patients after metastasectomy. The median disease-free and overall survivals after initial metastasectomy were 12 and 37 months, respectively, the 1-year, 3-year and 5-year disease-free survival (DFS) and overall survival (OS) rates being 46.5%, 24.3% and 17.9%and 92.3%, 59.0% and 39.0%, respectively. On multivariate analysis, the primary tumor site, tumor differentiation, resection margin and DFS were independent factors predicting better overall survival. Conclusions: In selected cases, hepatic metastasectomy for mCRC to the liver can result in long-term survival. Neoadjuvant chemotherapy did not exert a positive effect on DFS or OS. Adjuvant chemotherapy also did not appear to impact DFS and OS.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4339-4345
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• 2015

Occupational exposure to wood dust is one cause of nasopharyngeal cancer (NPC); however, assessing this exposure remains problematic. Therefore, the objective of this study was to develop a semi-quantitative exposure assessment method and then utilize it to evaluate the association between occupational exposure to wood dust and the development of NPC. In addition, variations in risk by histology were examined. A case-control study was conducted with 327 newly diagnosed cases of NPC at the National Cancer Institute and regional cancer centers in Thailand with 1:1 controls matched for age, gender and geographical residence. Occupational information was obtained through personal interviews. The potential probability, frequency and intensity of exposure to wood dust were assessed on a job-by-job basis by experienced experts. Analysis was performed by conditional logistic regression and presented in odds ratio (ORs) estimates and 95% confidence intervals (CI). Overall, a non significant relationship between occupational wood dust exposure and NPC risk for all subjects was observed (ORs=1.61, 95%CI 0.99-2.59); however, the risk became significant when analyses focused on types 2 and 3 of NPC (ORs=1.62, 95%CI 1.03-2.74). The significant association was stronger for those exposed to wood dust for > 10 year (ORs=2.26, 95%CI 1.10-4.63), for those with first-time exposure at age > 25 year (ORs=2.07, 95%CI 1.08-3.94), and for those who had a high cumulative exposure (ORs=2.17, 95%CI 1.03-4.58) when compared with those considered unexposed. In conclusion, wood dust is likely to be associated with an increased risk of type 2 or 3 NPC in the Thai population. The results of this study show that semi-quantitative exposure assessment is suitable for occupational exposure assessment in a case control study and complements the information from self-reporting.

• 한국산학기술학회논문지
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• 제21권10호
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• pp.421-432
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• 2020

본 연구의 목적은 초등학교 고학년 학생의 인터넷 게임 및 스마트폰 이용실태를 파악하고자 하였다. 2018년 10월 6일부터 2019년 9월 30일까지 K 지역 소재 16개 초등학교에 재학 중인 4~6학년 1,618명을 대상으로 자료를 수집하였고, 기술통계와 빈도분석, Chi-square를 이용하여 분석하였다. 연구결과, 인터넷 게임중독 자가진단척도(I-GUESS)는 고위험군이 6.1%로 나타났고, 스마트폰 과의존 척도(S-scale)는 잠재적 위험군 10.1%, 고위험군 0.8%로 나타났다. 인터넷 게임 및 스마트폰 사용과 관련된 신체적 문제는 36.0%가 경험했다고 응답했고, 눈의 피로가 20.4%로 가장 높았다. 정서적 문제는 18.5%에서 경험했다고 응답했으며, 분노가 6.8%로 가장 높았다. 사회적 문제는 21.8%가 경험한 것으로 파악되었고, 부모와의 갈등이 10.3%로 가장 높게 보고되었다. S-scale 점수와 I-GUESS 점수에 따른 주 사용 콘텐츠를 조사한 결과, S-scale 점수와 I-GUESS 점수가 높을수록 중계방송콘텐츠를 가장 많이 사용하는 것으로 나타났다. I-GUESS와 S-Scale 간의 상관관계에서는 I-GUESS와 S-Scale 간의 상관관계에서는 I-GUESS 점수가 고위험군 일수록, S-scale의 고위험군 비율도 높게 나타났으며, S-scale 점수가 고위험군 일수록 I-GUESS 점수의 고위험군 비율도 높은 것으로 나타났다. 향후, 이러한 변수를 고려하여 예방의 관점에서 초등학생을 위한 중재 프로그램 및 부모 교육 컨텐츠 개발이 시급함을 제언코자 한다.