• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.452-462
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• 2021

Pretilachlor (PRE) is a common acetanilide herbicide used worldwide. However, its effects on aquatic organisms, particularly marine photosynthetic life, are not sufficiently known. Herein, we evaluated the toxic effects of PRE by physiological and molecular parameters in the photosynthetic dinoflagellate Prorocentrum minimum. The cell density, pigment content, and photosynthetic parameters (F_v/F_m and PI_ABS) were considerably decreased with increased PRE exposure time and doses. In addition, photosynthesis-related genes, PmpsbA, PmpsaA, and PmatpB, were significantly upregulated when exposed to 1.0 mg L^-1 of PRE for 24 h (p<0.001). In 72 h treatment, the relative gene expression was significantly increased (0.1 and 0.5 mg L^-1; p<0.01). In contrast, PmrbcL was decreased or little changed compared to the controls. Reactive oxygen species (ROS) increased after 24 h exposure (p<0.001). However, the transcriptional fold-changes in glutathione S-transferase (GST) were significantly increased (0.5 and 1.0 mg L^-1; p<0.001) at 72 h. These findings suggested that the PmGST might be involved in PRE detoxification in P. minimum. In addition, PRE may affect the photosystem function in phytoplankton similar to other acetanilides, causing severe damage or cell death.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.286-291
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• 2003

Monochoria vaginalis is one of the most troublesome resistant weeds in Korean rice culture. Thus, the objectives of this study were to evaluate the response of M. vaginalis resistant to sulfonylurea(SU) herbicides and to determine alternative herbicides for the control of resistant M. vaginalis in direct seeded and transplanted rice culture in Korea. In greenhouse studies, the resistant biotype was 31-, 38-, 3172-, and 7-fold more resistant to ben-sulfuron-methyl, cyclosulfamuron, imazosulfuron, and pyrazosulfuron-ethyl, respectively, than the susceptible biotype, indicating cross-resistance to the SU herbicides used in this study. Non-SU herbicides, butachlor, carfentrazone-ethyl, mefenacet, pretilachlor, pyrazolate, and thiobencarb, several SU herbicide-based mixtures, ethoxysulfuron plus fentrazamide, pyrazosulfuron-ethyl plus pyrazolate plus simetryn, and non-SU herbicide-based mixtures, pyrazolate plus butachlor, pyrazolate plus pretilachlor, simetryn plus molinate, carfentrazone-ethyl plus butachlor, and carfentrazone-ethyl plus thiobencarb can be used to control both the resistant and susceptible biotypes of M. vaginalis when applied before the second leaf stage. In the field experiment, the resistant biotype of M. vaginalis that survived from the paddy fields treated with a SU herbicide-based mixture could effectively be controlled by using mixtures of bentazone plus MCPA, bentazone plus mecoprop-P, and bentazone plus 2,4-D when applied at 2 or 4 main leaves. Our results suggest that the SU-resistant M. vaginalis had not developed multiple resistances to herbicides with different modes of action. In particular, bentazone plus MCPA and bentazone plus mecoprop-P were effective control measures after failure to control resistant M. vaginalis in Korean rice culture.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.300-307
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• 2010

Greenhouse experiment was conducted to evaluate tolerance of six rice cultivars, three Indica${\times}$Japonica rice (long grain, cv. Dasanbyeo, Segejinmi and Hanareumbyeo) and three Japonica rice (short grain, Nampyung, Ilpumbyeo and Junamjosaeng) cultivars, to mesotrione+pretilachlor (MP) and bensulfuron-methyl+mesotrione+pretilachlor+pyriftalid (BMPP) in transplanting rice. Two herbicides were applied at 90 g and 180 g a.i. $ha^{-1}$ to three to four leaf stage rice at 5 and 15 days after transplanting, respectively. Related study was also conducted to compare $GR_{50}$ for Indica${\times}$Japonica and Japonica rice cultivars at different MP rates. Response to two herbicides varied with respect to rice cultivars and herbicide rates. All Indica${\times}$Japonica rice cultivars exhibited susceptible to both MP and BMPP as reflected by increased visual injury, shorter plant height and higher plant dry matter reduction when compared with nontreated rice cutlivars. The degree of foliar chlorosis by MP at 90 g a.i. $ha^{-1}$ was 5~6 at 7 days after treatment(DAT) but it was decreased to 3~6 at 14DAT. The degree of leaf chlorosis treated with 180 g a.i. $ha^{-1}$ MP was 6~7 at 7DAT and it was also decreased to 3~8 at 14 DAT. The plant hight of Indica${\times}$Japonica rices was inhibited by 18~43% at application of 90 g a.i. $ha^{-1}$ MP and 30~50% at 180 g a.i. $ha^{-1}$ MP. The dry matter reduction was greater than that of plant height inhibition, showing 46~73% at 90 g a.i. $ha^{-1}$ MP and 65~82% at 180 g a.i. $ha^{-1}$ MP. Similar leaf chlorosis injury and growth inhibition of rice cultivars was observed in the BMPP treatment. The injury and growth inhibition by MP and BMPP increased with increase in herbicide rate from 90 g a.i. $ha^{-1}$ to 180 g a.i. $ha^{-1}$. However, most of the Japonica cultivars are tolerant to MP and BMPP at both rates. There was no visible leaf chlorosis but plant height and dry matter production were slightly reduced at 180 g a.i. $ha^{-1}$. Based on $GR_{50}$ value determined in reduction of shoot dry weight for MP, the Indica${\times}$Japonica rice showed 12.9 fold lower compared with the Japonica rice. The result indicates that rice cultivars vary in tolerance to herbicides of MP and BMPP and Indica${\times}$Japonica rices were more susceptible than the Japonica rices to the MP and BMPP.

• Korean Journal of Environmental Agriculture
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• v.9 no.2
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• pp.83-95
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• 1990

This study was conducted to estimate influences of pesticides such as carbofuran[2,3-dihydro-2,2-dimethylbenzofuran-7-yl methyl carbamate] as an insecticide, and pyrazolate [4-(2,4-dichlorobenzolyl)-1,3-dimethyl-5-pyrazolyl-1,3-dimethyl-5-pyrazolyl-p-toluensulfonate], pyrazolate+pretilachlor [2-chlor-2,6-diethyl-N-(n-propoxyethyl) acetanilied] as herbicides on change in numbers of soil microorganisms and pH in planted and unplanted flooded rice paddy soils. The results of weekly investigated change of pH and populations of total bacteria, gram negative bacteria, anaerobic bacteria and fungi after treatments of pesticides were as follows : The change of pH in rice-planted soil gradually decreased in a matter of weeks after treatment with pesticide and the pH increased again from the sixth week, but no change of pH could be observed in nonplanted soil. The total numer of bacteria in the treated plots were slightly less than in the control plot, and the numbers decreased with increasing application rates of pesticides. But the microbial population increased in a matter of days after treatment with pesticide. Number of the gram negative bacteria until the sixth week after treatment of pesticide were fewer than control. The number in the carbofuran-treated plot decreased after a weeks after treatment, but numbers in plots treated with pyrazolate and pyrazolate+pretilachlor increased. The number of anaerobic bacteria in the treated plots were few by comparison with the untreated control, but the number increased after a weeks after treatment with pesticides. The populations of fungi in the carbofuran-treated plot were similar by comparison with the untreated control. The populations in the plots treated with pyrazolate and pyrazolate+pretilachlor decreased in 4 to 5 weeks with increase of application rate, but afterwards increased.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.63-72
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• 1985

To establish the method of integrated weed management in rice nurserybed, phytotoxic factors of herbicide and antidote efficacy were evaluated at the Yeongnam Crop Experiment Station in 1982 and 1984. Seven hundred eighty four research items were carried out as weed control research since 1961. While 65% of these were belonged to rice research, only 6% was attributed to nurserybed among rice research. More herbicidal phytotoxicity exhibited when seedbed was pressed just after seeding than sand covered or uncovered seedbed and also this phytotoxic symptom enhanced by using intact seed compared to pregerminated seed. Rinsing practice of seedbed reduced the phytotoxic effect of butachlor and this effect was more pronounced with the number of rinsing operation increase and at the pressed plot. However, herbicidal efficacy was not significantly decreased by rinsing operation. Growth of rice seedling hardly affected where the herbicide was absorbed through root only compared to absorption from both of root and shoot for pyrazolate, butachlor and thiobencarb. Herbicide antidote `CGA 123'407' completely protected from the phytotoxic effect of pretilachlor without arising any adversal effect in weed control. However, without antidote, pretilachlor showed the most severe phytotoxic symptom among used herbicides.

• Korean Journal of Environmental Agriculture
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• v.8 no.1
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• pp.7-16
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• 1989

Application of steam distillation on the analysis of pesticide residue in soil, water and crops was studied using the nitrogen distillation apparatus. Most pesticides which were extracted by organic solvents could be analyzed by the steam distillation method. For instance, distillations of PCNB, ${\gamma}-BHC$, ${\alpha}-or$ ${\beta}-endosulfan$, IBP, diazinon, fenthion, fenitrothion, alachlor, butachlor, pretilachlor, metolachlor, pendimethalin, benthiocarb and molinate were possible, but not simazine, atrazine and nitrofen. The optimum volume of distillate for a sufficient extraction of pesticide varied according to kind of pesticide. In general, the volume needed was little for carbamate, but much more for organochlorine. When the definite amount of distillate was obtained and then the condenser was washed by acetone, the optimum volume of distillate was less. Using the steam distillation method, the amount of organic solvent needed for one extraction of pesticide from soil, water and vegetable was less than the conventional solvent extraction method, and the analytical procedure became simpler. The process of concentration and clean up was mostly unnecessary, although a ghost peak was apparent in the gas chromatogram according to the kind of materials distilled.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.60-67
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• 1991

The experiment was conducted to evaluate weeding effect and to inhibit tuberization of Eleocharis kuroguwai authority by single or systematic applications of several herbicides. E. kuroguwai was controlled above 90 percentage by pretilachlor and butachlor+bensulfuron application at 5-10 DAT(Days after transplanting) followed by bentazon at 50 DAT and weeded 88 percentage by mefenacet+bensulfuron application at 15 DAT. Tuberization inhibition rate of E. kuroguwai was higher at the application of butachlor+bensulfuron at 5 DAT followed by bentazon at 50 DAT than any other applications. The weight variation of E. kuroguwai tubers formed at 15-20cm soil stratum was larger than that at 10-15cm. Tuber of E. kuroguwai was hardly formed at top soil stratum (0-5cm) and deep soil (15-20cm) by the systematic application of bentazon at 50 DAT. Grain yield of rice from systematic application with bentazon at 50 DAT and mefenacet+bensulfuron at 10-15 DAT was similar to that of hand weeded plot.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.248-253
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• 2013

This study was conducted to select alternative herbicides to control herbicide-resistant and - troublesome weeds in rice fields, Korea. The resistant Echinochloa oryzicola to ACCase inhibitor was tested by herbicides registered to control Echinochloa oryzicola. And the resistant and troublesome weeds to sulfonylurea (SU) herbicides, ALS inhibitors, were tested by widely using herbicides to control the resistant weeds in Korea. The oxadiazon, pyrazolate, pretilachlor and benzobicyclone+thiobencarb effectively controlled resistant Echinochloa oryzicola to ACCase and ALS inhibitors at 0.5 leaf stage of the, Herbicides containing mefenacet or fentrazamide controlled effectively by the 2 leaf stage. In controlling other SU resistant weeds including Sagittaria trifolia and Ludwigia prostrate, benzobicyclon and mesotrione performed well all tested weeds, and carfentrazone and pytazolate were effective to broadleaf weeds. Sagittaria trifolia and Ludwigia prostrate, which were suspected to be resistance to SU herbicides, were well controlled by carfentrazone and pytazolate.

• Weed & Turfgrass Science
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• v.4 no.3
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• pp.151-158
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• 2015

This study was conducted to establish the effective weed management methods in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, oxadiazon 12% EC, pyrazolate 36% SC, pretilachlor 14% EC and thiobencarb 50% EC were effective until 0.5 leaf stage of herbicide resistant Echinochloa oryzoides. Herbicides registered for use after transplanting, fentrazamide 1% GR and mefenacet 18% SC were effective until 2 leaf stage of herbicide resistant Echinochloa oryzicola and triafamone 0.98% SC was possible to control up to 4 leaf stage. HPPD inhibitors, benzobicyclon, mesotrione and tefuryltrione SC, were simultaneously effective to SU herbicide-resistant Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, benzobicyclon + oxadiargyl EC out of the tested herbicide was most effective in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Its effectiveness rises in proportion to flooding duration. Mazosulfuron GR, a herbicides registered for use after transplanting was most effective without phytotoxicity until 60 days after transplanting in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola.

• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.9-22
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• 2020

This study was designed to use the safety management data for residual pesticides in imported banana based on the investigation of pesticide residue detection of agricultural products with different origins in the Republic of Korea. From the USA, EU, UK, Japan and Korea from 2007 to 2018, the results of banana residue pesticides were summarized into detected pesticides, number of inspections, number of pesticide detection cases, and the amount of detected pesticide residue. A total of 109 pesticides were detected for the pesticide residue and pesticide detection rate was 4.58% in 206,894 cases. The detection rate was ranged within 10.62-24.62% for chlorpyrifos, imazalil, methyl-bromide, azoxystrobin, carbendazim, pretilachlor and thiabendazole. Among them, chlorpyrifos was detected most often followed by imazalil, azoxystrobin, thiabendazole, bifenthrin and carbendazim. According to the results of monitoring data for bananas in EU, Japan, USA, UK and Korea, the kinds of detected pesticides were 85, 57, 23, 18 and 8, respectively. Azoxystrobin, bifenthrin and chloropyrifos were found in monitoring data of all countries. Fourteen and twelve pesticides were detected in bananas from Costa Rica and Ecuador, respectively. Imazalil and thiabendazole were detected in 16 and 11 origins, respectively. Myclobutanil and iprodione were detected in four and two countries, respectively. In bananas from Costa Rica, azoxystrobin and bifenthrin were detected 11.8 and 9.8%, respectively, and the detection rate of azoxystrobin was 19% in bananas from Colombia. Chlorpyrifos was detected 22.7, 13.3 and 10.8% in bananas from Belize, Colombia and Costa Rica respectively. Myclobutanil was detected in bananas from Colombia and Costa Rica with the rate of 17.9 and 10.4%, respectively.