• Parasites, Hosts and Diseases
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• v.50 no.1
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• pp.83-87
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• 2012

To determine the effects of kimchi extracts at different temperatures on larval development, $Ascaris$ $suum$ eggs were mixed with soluble part of 7 different brands of commercially available kimchi and preserved at either $5^{\circ}C$ or $25^{\circ}C$ for up to 60 days. $A.$ $suum$ eggs incubated at $25^{\circ}C$ showed marked differences in larval development between kimchi extract and control group. While all eggs in the control group completed embryonation by day 21, only 30% of the eggs in the kimchi extract group became embryonated by day 36 and about 25% never became larvated even at day 60. At $5^{\circ}C$, however, none of the eggs showed larval development regardless of the incubation period or type of mixture group. To determine the survival rate of $A.$ $suum$ eggs that showed no embryonation after being preserved at $5^{\circ}C$, eggs preserved in kimchi extracts for 14, 28, and 60 at $5^{\circ}C$ were re-incubated at $25^{\circ}C$ for 3 weeks in distilled water. While all eggs in the control group became larvated, eggs in the kimchi extract group showed differences in their embryonation rates by the incubation period; 87.4 % and 41.7% of the eggs became embryonated after being refrigerated for 14 days and 28 days, respectively. When refrigerated for 60 days, however, no eggs mixed in kimchi extract showed larval development. Our results indicate that embryogenesis of $A.$ $suum$ eggs in kimchi extract was affected by duration of refrigeration, and that all eggs stopped larval development completely in kimchi kept at $5^{\circ}C$ for up to 60 days.

• Journal of the Korean earth science society
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• v.31 no.1
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• pp.8-17
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• 2010

Geology and stromatolite fossil localities of Socheong Island are reviewed on the basis of previous studies and recent field survey. A new fossil locality of stromatolites which are very well preserved is recognized in Bunam area, northeast of Socheong Island. An outcrop composed of sandstone and shale alternations which exhibit well-developed laminations and folds is also found in the east of the Socheong harbor, and it is regarded as one of the symbolic features of the geology in Socheong Island. Stromatolite fossil localities of Socheong Island contains diverse and well-preserved Late Proterozoic stromatolites which are correlated with those from the Mukchon and Myoraksan series of the Sangwon System and diverse sedimentary structures such as ripple marks, desiccation cracks, and raindrop imprints. Stromatolites of Socheong Island, the oldest fossil Natural Monument of Korea, should be preserved at the national level, and continued intensive research must be conducted.

• Journal of the Korean Wood Science and Technology
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• v.46 no.6
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• pp.748-755
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• 2018

Timber from fast-growing tree species is susceptible to by biodeterioration attack, particularly subterranean termites. Impregnation with methyl methacrylate (MMA) potentially increases wood resistance to subterranean termite attack. Four wood species, namely sengon (Falcataria moluccana), jabon (Anthocephalus cadamba), mangium (Acacia mangium), and pine (Pinus merkusii), were impregnated with MMA, and samples of untreated and imidacloprid-preserved wood were prepared for comparison purposes. Small stakes, sized 0.8 cm by 2 cm in cross section by 20 cm in the longitudinal direction, were inserted into the ground for 3 months, and the weight loss of each specimen was determined at the end of the test period. A factorial $4{\times}3$ completely randomized design was used for data analysis; the first factor was wood species, and the second factor was treatment. The results showed that MMA polymer loadings were 27.88%, 24.91%, 14.14%, and 17.81% for sengon, jabon, mangium, and pine, respectively, and amounts of imidacloprid retention were $7.56kg/m^3$, $5.98kg/m^3$, $5.34kg/m^3$, and $9.53kg/m^3$, respectively. According to an analysis of variance, wood species, treatment, and interaction of both factors significantly affected the weight loss of wood specimens. Mangium had the smallest weight loss, followed by pine, sengon, and jabon. MMA impregnation into the wood increased the resistance of wood samples to subterranean termite attack during in-ground testing, but the resistance level was lower than that of imidacloprid-preserved wood. Except for mangium wood, the MMA treatment did not significantly affect resistance.

• Journal of Embryo Transfer
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• v.28 no.2
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• pp.113-119
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• 2013

The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature ($4{\sim}5^{\circ}C$) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=-0.997; P<0.05) and % HYP motile spermatozoa (r=-0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn't remain useable after certain period of preservation with respect to its motility and morphology.

• Ocean and Polar Research
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• v.36 no.4
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• pp.423-435
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• 2014

Verifying the similarity of environmental characteristics between an artificial impact site and a preserved or reference site is necessary to quantitatively and qualitatively evaluate the environmental impact of mining activity. Although an impact site (BIS station) and a preserved site (called KOMO station) that have been selected in the Korea manganese nodule contract area may share similar environmental characteristics, similarities in terms of the water column environment between both sites has not been investigated. In this study, we compared the chemical properties of the water columns and sinking particle fluxes between BIS and KOMO stations through two observations (August 2011 and September 2012). Additionally, we observed particle fluxes at the KOMO station for five years (July 2003~July 2008) to understand long-term natural variability. Vertical distributions of water column properties such as dissolved oxygen, inorganic nutrients (N, P, Si), total organic carbon below surface layer (within the depth range of 200 m) were not considerably different between the two sites. Especially, values of water column parameters in the abyssopelagic zone from 4000 m to bottom layer (~5000 m) were very similar between the BIS and KOMO sites. Sinking particle fluxes from the two sites also showed similar seasonality. However, natural variation of particle flux at the KOMO site varied from 3.5 to $129.9mg\;m^{-2}day^{-1}$, with a distinct temporal variation originating from ENSO events (almost forty times higher than a minimum value). These results could provide valuable information to more exactly evaluate the environmental impact of mining activity on water columns.

• Culinary science and hospitality research
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• v.23 no.5
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• pp.25-33
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• 2017

This study was to investigate the effects of enzyme on quality characteristics and antioxidant activities of wild peach (Prunus persica L.) juice. pH levels and S.S (soluble solid) values in all samples ranged from 3.86 to 4.13 and from 48.0 to $55.0^{\circ}Brix$, respectively. The TA (total acidity) values of control (not treatment enzyme) were higher than those of the others. The highest 'L', 'a' and 'b' values were observed on PWP (preserved wild peach (Prunus persica L.) juice of cellulase/pectinase (1:1, w/w) sample. Glucose (26.65 g/100 g) and fructose (17.42 g/100 g) in PWP product were determined, however sucrose and maltose were not detected. The predominating organic acid components analyzed in PWP sample were tartaric (32.36 g/100 g) and lactc acids (209.34 g/100 g), whereas citric acid, acetic acid and malic acid were not detected. Higher scores for taste, flavor, color and overall acceptance were found for PWP products compared to other samples. The total phenolic content (13.31 mg GAE/mg dry weight) analyzed using Folin-Ciocalteu's reagent, of PWP sample was higher than those of the others and the total flavonoid concentrations were also 10.95 mg CE/mg dry weight. The DPPH radical scavenging and ABTS radical scavenging activities in all samples ranged from 55.16 to 74.29% and from 39.59 to 82.79%, respectively. The antioxidant activities were affected by addition of enzyme. These results indicate that the use of the mixture of cellulase and pectinase could increase the quality, and antioxidant potentials of sugar preserved wild peach (Prunus persica L.) juice by enzymatic treatment.

• Archives of Plastic Surgery
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• v.35 no.6
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• pp.716-722
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• 2008

Purpose: Split or full thickness skin graft is generally used to reconstruct the palmar skin and soft tissue defect after release of burn scar flexion contracture of hand. As a way to overcome and improve aesthetic and functional problems, the authors used the preserved superficial fat skin(PSFS) composite graft for correction of burn scar contracture of hand. Methods: From December of 2001 to July of 2007, thirty patients with burn scar contracture of hand were corrected. The palmar skin and soft tissue defect after release of burn scar contracture was reconstructed with the PSFS composite graft harvested from medial foot or below lateral and medial malleolus, with a preserved superficial fat layer. To promote take of the PSFS composite graft, a foam and polyurethane film dressing was used to maintain the moisture environment and Kirschner wire was inserted for immobilization. Before and after the surgery, a range of motion was measured by graduator. Using a chromameter, skin color difference between the PSFS composite graft and surrounding normal skin was measured and compared with full thickness skin graft from groin. Results: In all cases, the PSFS composite graft was well taken without necrosis, although the graft was as big as $330mm^2$(mean $150mm^2$). Contracture of hand was completely corrected without recurrence. The PSFS composite graft showed more correlations and harmonies with surrounding normal skin and less pigmentation than full thickness skin graft. Donor site scar was also obscure. Conclusion: The PSFS composite graft should be considered as a useful option for correction of burn scar flexion contracture of hand.

• Reproductive and Developmental Biology
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• v.39 no.1
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• pp.7-11
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• 2015

Preservation of liquid semen is an important factor for breeding management in swine industry. Oxidative stress of spermatozoa during liquid preservation has a detrimental effect on sperm quality and decreases fertility. Objective of this study was to determine the effect of antioxidant, Quercetin, on capability of porcine liquid semen preservation. Freshly collected porcine semen from boars (n=3), having proven fertility was counted, diluted to $3{\times}10^7/mL$ and divided into 5 different semen extenders. Aliquots of diluted semen with different extenders were subjected to measure the pH, motility, viability and sperm DNA structure status on elapse time after preservation for 10 days. For the first 3 days, semen preserved in all 5 different extenders maintained their initial pH and either gradually decreased or increased thereafter, indicating lipid peroxidation has started. Sperm motility (r=0.52, p=0.01) and viability (r=0.55, p=0.03) had positive correlation with semen pH. Sperm motility was maintained well (p<0.05) in especially 2 extenders containing Tris and antioxidant compared to other extenders, suggesting both Tris and antioxidant worked as pH regulator and had beneficial effects on sperm characteristic during preservation. Sperm DNA structure status accessed by sperm chromatin structure assay on elapsed time after preservation, tended to be higher in semen preserved without antioxidant. Taken together, addition of antioxidant to extender prevents the sperm from oxidative stress during storage in mechanism by which antioxidant slows the lipid peroxidation, and thus reduced the reactive oxygen species in preserved porcine semen resulted in maintaining semen pH, sperm motility and viability for 7~10 days.

• The Korean Journal of Mycology
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• v.39 no.2
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• pp.141-144
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• 2011

Short-preservation of basidiomycetes is generally being conducted in slant tubes containing solid medium based on agar. In this study, we investigated the vitality of 28 species and 76 strains preserved on potato dextrose agar (PDA) at $4^{\circ}C$ for 2~7 years. The survival rates of the fungi were 82%, 86%, 94%, 96%, 94%, and 94% for seven, six, five, four, three, and two years old preservation, respectively. The volume of medium in Lentinula edodes showed decrease after 2 years preserved. The pH of preserved medium was 5.42 in 2007 (two years old), but it became nearly neutral as increasing preservation term.

• 한국지구과학회:학술대회논문집
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• 2010.04a
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• pp.107-107
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• 2010

Geology and stromatolite fossil localities of Socheong Island are reviewed on the basis of previous studies and recent field survey. A new fossil locality of stromatolites which are very well preserved is recognized in Bunam area, northeast of Socheong Island. An outcrop composed of sandstone and shale alternations which exhibit well-developed laminations and folds is also found in the east of the Socheong harbor, and it is regarded as one of the symbolic features of the geology in Socheong Island. Stromatolite fossil localities of Socheong Island contains diverse and well-preserved Late Proterozoic stromatolites which are correlated with those from the Mukchon and Myoraksan series of the Sangwon System and diverse sedimentary structures such as ripple marks, desiccation cracks, and raindrop imprints. Stromatolites of Socheong Island, the oldest fossil Natural Monument of Korea, should be preserved at the national level, and continued intensive research must be conducted.