• Journal of Environmental Health Sciences
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• v.48 no.1
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• pp.36-43
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• 2022

Background: Preservatives are used to prevent product deterioration in modeling clay. Parabens, a representative preservative, have been found to be endocrine disruptors and cause skin irritation and allergic reactions. Isothiazolinone preservatives can be irritating to the skin, respiratory tract, and eyes. Thorough investigation and regulation of clay are necessary because clay is marketed to children, who are more sensitive to the toxic effect of chemicals. Objectives: In this study, the presence of 16 preservatives was analyzed in modeling clay and the results were compared with current standards. Methods: A total of 200 samples were collected from 28 children's clay products sold in South Korea (13 from Korea and 15 imported from overseas). Twelve preservatives, such as parabens, were analyzed using high-performance liquid chromatography (HPLC). Isothiazolinone preservatives (chloromethylisothiazolinone; CMIT, methylisothiazolinone; MIT, octylisothiazolinone; OIT, and benzisothiazolinone; BIT) were analyzed using ultra performance liquid chromatography-tandem mass spectrometery (UPLC-MS/MS). Results: Dehydroacetic acid (DHA) was detected the most in the clays at 51.50% (103 cases) detection; 38 cases (median 190.42 ㎍/g) in Korean products and 65 cases (median 169.62 ㎍/g) in Chinese products. CMIT, which is prohibited in Korea, was detected in 14 (median 16.28 ㎍/g) Chinese products. OIT, which has a chemical structure similar to CMIT was found in 28 (median 68.38 ㎍/g) samples in Korean products. Conclusions: The use of CMIT and MIT in children's products is prohibited in Korea and the European Union (EU). The detection of CMIT in Chinese clay products suggests that management is necessary for imported products. It is necessary to review the safety and regulatory status for OIT because OIT was used as a substitute for CMIT and MIT in Korean products.

• Journal of People, Plants, and Environment
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• v.22 no.6
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• pp.583-591
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• 2019

This study was conducted to examine the effects of wet storage solution, transport period and temperature on vase life and quality of cut flowers in standard chrysanthemum 'Jinba'. Immediately after transport, the fresh weight and flower diameter of cut flowers did not show a difference according to wet storage solutions regardless of the transport period, but as the transport period increased, the fresh weight and flower diameter increased. The flower bud stage at harvest was maintained due to the small changes in flower diameter, and the freshness of leaves was better when transported at 5℃ than at 25℃. When transported at 25℃, the longer the transport period, the lower the quality of cut flowers as some petals opened up and showed early flowering after transport. In preservative solutions, quality of cut flowers transported at 25℃ was lower than that at 5℃ due to fresh weight and diameter according to the longer transport period. The vase life of cut flowers was 1.0 day, 0.8 day, and 7.3 days longer when transported for 3, 5, and 7 days respectively at 5℃ than at 25℃. The quality of cut flowers was better due to increase in fresh weight and flower diameter, as well as vase life in wet storage solutions of ClO₂ and Chrysal OVB than in tap water, regardless of transport period and temperature. There was no difference in fresh weight and vase life between ClO₂ and Chrysal OVB, but flower diameter was greater in ClO₂ than in Chrysal OVB. Therefore, for long-term transport of cut standard chrysanthemum 'Jinba', wet storage transport in ClO₂ at 5℃ was found effective in maintaining the quality and vase life of cut flowers.

• Korean Journal of Ecology and Environment
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• v.56 no.4
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• pp.303-319
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• 2023

Pitfall traps that use ethylene glycol as a preservative solution are commonly used in arthropod research. However, a recent surge in cases involving damage to these traps by roe deer or wild boars owing to the sweet taste of ethylene glycol has prompted the addition of quinone sulfate, a substance with a pungent taste, to deter such wildlife interference. This study aimed to assess the effects of quinone sulfate on arthropods collected from pitfall traps containing ethylene glycol. We strategically positioned 50 traps using ethylene glycol alone and 50 traps containing a small amount of quinone sulfate mixed with ethylene glycol in a grid pattern for systematic sampling at the Gwangneung Forest long-term ecological research (LTER) site. Traps were collected 10 days later. The results revealed a notable effect on ants when quinone sulfate was introduced. Specifically, it decreased the number of ants. In a species-specific analysis of ants, only Nylanderia flavipes showed a significant decline in response to quinone sulfate, whereas other ant species remained unaffected. Additionally, among the arthropod samples obtained in this survey, we identified species or morpho-species of spiders, beetles, and ants and assessed species diversity. Consequently, the utilization of quinone sulfate should be undertaken judiciously, taking into account the specific species composition and environmental characteristics of the monitoring site. Our study also highlighted the significant response of various arthropod groups to variations in leaf litter depth, underscoring the crucial role of the leaf litter layer in providing sustenance and shelter for ground-foraging arthropods. Furthermore, we have compiled comprehensive species lists of both spiders and ants in Gwangneung forest by amalgamating data from this investigation with findings from previous studies.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.59-59
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• 2020

수국 '베레나 그린'의 줄기하단의 엽을 제거하고 5엽 상태에서 수돗물과 0.1% 크리잘 RVB에 각각 담가 4℃에서 24시간 물올림을 하고 물올림을 하고, 줄기를 60~70cm 길이로 끝을 대각선으로 절단하여 물올림용액을 채운 물 대롱을 끼운 다음 꽃을 보호하기 위한 비닐 슬리브를 씌워 유통용 종이상자에 담아 5±1℃ 냉장차로 국립원예특작과학원으로 운반하였으며, 절화수명 및 관련 특성분석을 위해 평가실로 옮기기 전까지 유통용 종이상자에 담긴 상태로 저온저장고(5±1℃)에 보관하였다. 보존용액에 따른 절화특성 조사를 위해 절화를 평가실에 전시하기 전에 절화의 줄기길이를 40cm로 맞춰 절단한 뒤에 보존용액을 800 mL 채운 플라스틱 화병에 꽃아 절화 화관끼리 서로 닿지 않도록 배치하였으며 22±2℃ 실내에서 절화수명을 조사하였다. 보존용액으로 4% 차아염소산나트륨 용액, 1% sucrose + 250 mg/L 8-hydroxquinoline + 100 mg/L citric acid로 이루어진 용액(HQ) 그리고 시중에서 수국 절화 보존제로 판매되는 크리잘 프로 II, III와 크리잘 clear 0.5% 용액을 사용하였다. 수돗물에 물올림하였을 때 보존용액인 0.5% 크리잘 프로 II에서는 33.8일, 0.5% 크리잘 프로 III에서는 27.7~33.5일, 크리잘 클리어는 33.7일, 차아염소산나트륨과 수돗물은 각각 26.2, 28.8일이었고, HQ 용액은 49.0일로 크리잘보다 15일, 수돗물보다 20일 절화수명이 연장되었다. 수국 '베레나 그린'의 보존용액으로 1% sucrose + 250 mg/L 8-hydroxquinoline + 100 mg/L citric acid를 사용하는 것이 관상기간을 연장하는 데 효과적이었다.

• Journal of the Korean Wood Science and Technology
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• v.52 no.2
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• pp.134-144
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• 2024

This study aimed to analyze the anti-termite properties of tamanu (Calophyllum inophyllum L.) stem bark extracts against subterranean termites, specifically, Coptotermes curvignathus. The bark powder of C. inophyllum was extracted using different solvents, such as n-hexane, ethyl acetate, and methanol, using the maceration method. Anti-termite testing was performed using two paper disc methods: no- and two-choice tests. Whatman test paper was dripped with the extract solutions at concentrations of 4%, 6%, 8%, and 10% (w/v). Subsequently, the treated paper disc was placed into an acrylic tube, and the subterranean termite was added. The parameters utilized in the test included termite mortality and the weight loss of the test paper. The results revealed that the total extract yield of C. inophyllum stem bark was 30.24%. Furthermore, the extractive substances from C. inophyllum bark exhibited anti-termite activity. The most favorable outcomes were obtained with the n-hexane and ethyl acetate extracts at a concentration of 10%. The termite mortality and weight loss of the test paper were respectively 66% and 5.67% for the n-hexane extract and 66.67% and 6.19% for the ethyl acetate extract. In addition, the n-hexane extract contained friedelan-3-one, while the ethyl acetate extract contained 1,2-benzene dicarboxylic acid, dinonyl ester, and friedelan-3-one. The results suggested that these compounds are responsible for the observed anti-termite activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.12
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• pp.1640-1646
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• 2008

In the current food sanitation regulation, food additives are under controlled by the Food Code. The naturally derived preservatives such as benzoic acid and propionic acid can be naturally carried over or produced as metabolites during manufacturing process such as fermentation. To monitor naturally formed benzoic acid and propionic acid levels, a total of 145 samples were classified into berries (prune, cranberry), functional foods (propolis liquid, ginseng product), vinegars (vinegar-based drink, vinegar beverage, vinegar), and salted and pickled products (olive, pickled cucumber, salted/pickled product) and analyzed by HPLC-PDA and GC-FID. From the results, benzoic acid and propionic acid were each detected and identified in 144 samples and 64 samples respectively. The amount of benzoic acid ranged from $4.1{\sim}478.4\;ppm$ in cranberry, from $49.7{\sim}491$ in propolis liquid, and from $2.5{\sim}10.2\;ppm$ in ginseng, and other tested samples contained very small quantity. Also, the amount of propionic acid ranged from $179.8{\sim}951.9\;ppm$ (av. 553.6 ppm) in vinegar (persimmon vinegar 100%), which was the highest level among fermented foods, from $13.7{\sim}247.0$ ppm in propolis liquid, from $2.0{\sim}180.7\;ppm$ in vinegar-based drink, and from $1.6{\sim}76.6\;ppm$ in olive. Vinegar beverage and pickled cucumber each showed 24 and 18 ppm of propionic acid; in contrast, propionic acid was not detected in prune, cranberry, ginseng, and picked/salted products.

• Journal of Food Hygiene and Safety
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• v.25 no.3
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• pp.232-237
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• 2010

This study was carried out to evaluate the safety of circulated oriental medicines and their related preparations in Gyeonggi-do. Total 366 samples (165 species) were analyzed about heavy metals, residual contents of sulfur dioxide ($SO_2$), and 68 samples were analyzed about preservatives. 17 samples (13 species, 4.6%) were exceeded the legal limit of heavy metal. The concentrations over the legal limit for Pb, Cd, As and Hg were 6.1~19.2 mg/kg, 0.4~0.7 mg/kg, 6.9 mg/kg and 0.7 mg/kg, respectively. In particular, the exceeding ratio (5.9%) of the legal limit of heavy metals in foreign products was 3.3 times more than domestic products (1.8%). 13 samples (10 species, 3.6%) exceeded the limit of residual sulfur dioxide and the concentration ranges were 105 to 428 mg/kg in domestic products, on the other hand foreign products were from 114 to 2,468 mg/kg. The mean concentration over the limit of residual sulfur dioxide of foreign products (804 mg/kg) was 2.4 times more than domestic products (338 mg/kg). In studying of the preservatives in oriental preparation, the contents of dehydroacetic acid (48.9~64.1%) in 3 samples of labeled solutions were under the labeled preservative contents and the contents of benzoic acid (139.9%) in 1 sample of labeled pill product was exceeded the labeled preservative contents. The numbers of the detected preservatives in unlabeled solutions, pills and granules were 4, 11 and 7, respectively.

• Microbiology and Biotechnology Letters
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• v.42 no.4
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• pp.361-366
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• 2014

In this study, Glycyrrhiza uralensis and Glycyrrhiza glabra extracts, with their countries of origin as Korea (Jecheon), Uzbekistan and China, were prepared under various extraction conditions. There were 8 extraction conditions which the licorice were subjected to, and all conditions had different extraction solvents, temperatures and times. Antimicrobial activity on skin flora was evaluated comparatively by a disc diffusion assay, broth macrodilution assay, and kill time curve assay. Based on the antimicrobial activity of their extract confirmed by disc diffusion assay, we established optimal extraction conditions. The Korean licorice extract (85% ethanol, $40^{\circ}C$, 12 h) showed the best activity amongst the samples examined. In particular, its antimicrobial activity against Propionibacterium acnes was the highest. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the licorice extracts revealed that the Korean licorice ($156{\mu}g/ml$ and $1,250{\mu}g/ml$) had better antimicrobial activity than that of the Uzbekistani licorice ($625{\mu}g/ml$ and $2,500{\mu}g/ml$) and the Chinese licorice ($625{\mu}g/ml$ and $5,000{\mu}g/ml$). Taken together, it was shown that Korean licorice extracted in group F (85% ethanol, $40^{\circ}C$, 12 h) had the highest antimicrobial activity amongst the licorices from the other countries of origin. These results also suggest that the optimal extraction conditions are 85% ethanol, $40^{\circ}C$, 12 h, and that licorice has a potential application as a natural preservative in cosmetics products, thereby replacing synthetic preservatives.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.1
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• pp.20-35
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• 2023

Chemical preservatives have a good effect on antibacterial activity, but many side effects on the human body have been reported. Recently, the development of natural preservatives that are harmless to the human body and have preservative functions and self-efficacy is active. In addition, in order to increase the absorption rate of natural products by the human body, the method of fermentation using strains is also increasing. Therefore, this study selected varieties that are harmless to the human body and have good antibacterial activity. 1. The yield of origin, thickness and solvent was investigated. Scutellaria baicalensis Georgi was made in China and received a yield of 21.88% from 50% ethyl alcohol extract. Salvia miltiorrhiza Bunge was made in Korea and received a yield of 25.62% from 50% ethyl alcohol extract. Dryopteris crassirhizoma Nakai was made in China and received a yield of 6.50% from 70% ethyl alcohol extract. 2. The solid fermentation with the S. baicalensis and S. miltiorrhiza with B. Subtilis yield gained 24.40%, 39.30%, and D. crassirhizoma obtained 11.10% yield when fermented with L. casei. 3. After the liquid fermentation, a clear zone of 9mm was identified for the S. aureus strain in the S. baicalensis, and the antibacterial activity was not confirmed in S. miltiorrhiza and D. crassirhizoma. 4. When the S. baicalensis was fermented with L. Casei, it showed high antibacterial activity in C. albicans and S. aureus. S. miltiorrhiza showed antibacterial activity in S. aureus when it was solid with S. cerevisiae. When the spectators were solid with L. casei and S. cerevisiae, antibacterial activity was high in E. coli and S. aureus. Overall, the antibacterial activity after fermentation was much higher than when fermented. 5. The change in active ingredients was baicalin 101.57, baicalein 28.26, and wogonin 5.33mg/g in the S. baicalensis that did not ferment solid. When solid fermentation with S. cerevisiae, the content of baicalinin with baicalin 94.31, baicalein 30.41, and wogonin 3.57mg/g was found to have increased. S. miltiorrhiza that was not fermented, salvianolic acid A was 1.82mg/g, and when fermented with S. cerevisiae, it increased to 5.70mg/g. The active ingredients of the spectators were flavaspidic acid AP, flavaspidic acid PB, flavaspidic acid AB, and flavaspidic acid BB.

• Korean Chemical Engineering Research
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• v.61 no.2
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• pp.302-311
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• 2023

This study was conducted to evaluate the applicability of castor oil (CSO) as a natural wood preservative. CSO was treated into wood blocks prepared with domestic and imported wood species using a vacuum-pressure method, and then treatability, leachability and decay resistance of the CSO-treated wood blocks were examined. Although CSO was penetrated effectively into wood blocks of all wood species, the CSO-treatability was the highest in Western hemlock, followed by Japanese larch (LA), soft maple and Mongolian oak due to the difference of its anatomical structure. Except for LA, the more retained, the more leached during a saline water-immersing process for 48h. The use of ethanol added to reduce the viscosity of CSO affected negatively the treatability and leachability of wood blocks. Decay resistance, which was evaluated by the weight loss of wood blocks exposed against Fomitopsis palustris (FOP) and Trametes versicolor, of the CSO-treated/leached wood blocks was superior to that of control. Especially, most of wood blocks treated with preserving solution composed of only CSO (CSO-2) did not decayed and showed a very low weight loss against FOP. The decay resistance results from CSO retained in wood blocks after leaching. The retention of CSO could identify using the observation of X-ray microscope. Length of wood strips, which were treated with CSO-2 and then immersed in saline water for 2 weeks, hardly changed in all cutting directions. In addition, weight gain and length-swelling rate of the wood strips were extremely low compared to those of control. These results indicate that moisture resistance of the wood strips was improved by the CSO treatment. It is concluded that the treatment of CSO using a vacuum-pressure method provides the decay resistance and dimensional stability of wood, and thus CSO can be used as a natural wood preservative on various indoor and outdoor circumstances.