Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Comparison of Substance Change and Antibacterial Activity Before and After Fermentation Using Resource Plants for The Development of Natural Preservatives

천연방부제 개발을 위한 자원식물을 활용한 발효 전·후 물질 변화와 항균활성 비교

  • Seo A Jung (Sooy-K Bio Lab, Research and Development) ;
  • Youn Ok Jung (Sooy-K Bio Lab, Research and Development) ;
  • Ga Hyeon Song (Sooy-K Bio Lab, Research and Development) ;
  • No Bok Park (Department of Floriculture, Korea National College of Agriculture and Fisheries)
  • 정서아 (수이케이 바이오랩 연구소) ;
  • 정연옥 (수이케이 바이오랩 연구소) ;
  • 송가현 (수이케이 바이오랩 연구소) ;
  • 박노복 (국립한국농수산대학교 원예학부)
  • Received : 2023.02.23
  • Accepted : 2023.03.24
  • Published : 2023.03.30
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Abstract

Chemical preservatives have a good effect on antibacterial activity, but many side effects on the human body have been reported. Recently, the development of natural preservatives that are harmless to the human body and have preservative functions and self-efficacy is active. In addition, in order to increase the absorption rate of natural products by the human body, the method of fermentation using strains is also increasing. Therefore, this study selected varieties that are harmless to the human body and have good antibacterial activity. 1. The yield of origin, thickness and solvent was investigated. Scutellaria baicalensis Georgi was made in China and received a yield of 21.88% from 50% ethyl alcohol extract. Salvia miltiorrhiza Bunge was made in Korea and received a yield of 25.62% from 50% ethyl alcohol extract. Dryopteris crassirhizoma Nakai was made in China and received a yield of 6.50% from 70% ethyl alcohol extract. 2. The solid fermentation with the S. baicalensis and S. miltiorrhiza with B. Subtilis yield gained 24.40%, 39.30%, and D. crassirhizoma obtained 11.10% yield when fermented with L. casei. 3. After the liquid fermentation, a clear zone of 9mm was identified for the S. aureus strain in the S. baicalensis, and the antibacterial activity was not confirmed in S. miltiorrhiza and D. crassirhizoma. 4. When the S. baicalensis was fermented with L. Casei, it showed high antibacterial activity in C. albicans and S. aureus. S. miltiorrhiza showed antibacterial activity in S. aureus when it was solid with S. cerevisiae. When the spectators were solid with L. casei and S. cerevisiae, antibacterial activity was high in E. coli and S. aureus. Overall, the antibacterial activity after fermentation was much higher than when fermented. 5. The change in active ingredients was baicalin 101.57, baicalein 28.26, and wogonin 5.33mg/g in the S. baicalensis that did not ferment solid. When solid fermentation with S. cerevisiae, the content of baicalinin with baicalin 94.31, baicalein 30.41, and wogonin 3.57mg/g was found to have increased. S. miltiorrhiza that was not fermented, salvianolic acid A was 1.82mg/g, and when fermented with S. cerevisiae, it increased to 5.70mg/g. The active ingredients of the spectators were flavaspidic acid AP, flavaspidic acid PB, flavaspidic acid AB, and flavaspidic acid BB.

화학방부제는 항균활성은 좋지만 많은 부작용이 보고되고 있어 최근에는 인체에 해가 없으며 방부 기능과 자체 효능이 있는 천연방부제의 개발이 활발하다. 또한 천연물의 흡수율을 높이기 위한 방법으로 분자량이 커서 인체에 잘 흡수되지 않아 저분자화를 시키기 위한 방법으로 균주를 이용하여 발효하는 것도 늘어나고 있는 추세이다. 이에 천연방부제가 방부 효과는 지속되어야 하고 첨가했을 때 품질에 손상을 주지 않아야 하며, 향, 색과 성분에 영향을 끼치지 않아야 한다는 요건들을 충족시켜야 한다. 따라서 본 연구는 인체에 해가 없으며 항균활성이 좋은 황금, 단삼, 관중을 선정하였다. 식물은 모두 에탄올로 추출하였고, 발효균주로는 B. subtilis, L. casei, S. cerevisiae와 누룩에서 분리한 Asp. oryzae로 액체와 고체발효를 하여 발효 전과 발효 후의 유효성분의 함량을 비교하였다. 항균활성을 알아보기 위해 C. albicans, S. aureus, P. aeruginosa 세균 3종과 진균은 E. coli로 억제력을 탐색하였다. 천연방부제 개발로 사용하기 위해서는 항균활성이 있고 이러한 실험을 수행하여 다음과 같은 결과를 얻었다. 1. 원산지, 굵기와 용매에 따른 수율은 황금은 중국산이며 굵기는 2,800㎛로 50% ethyl alcohol 추출물에서 21.88%, 단삼은 국내산으로 굵기는 2,800㎛이며 50% ethyl alcohol 추출물에서 25.62%, 관중은 중국산으로 굵기는 2,800㎛, 70% ethyl alcohol 추출물에서 6.50%의 수율을 얻었다. 2. L. casei로 1일 액체 발효했을 때 수율은 황금 66.8%, 단삼 84.8%, 관중 73.0%였다. B. subtilis로 고체발효한 황금과 단삼은 24.40%, 39.30%, 관중은 L. casei로 발효 했을 때 11.10%의 수율을 얻었다. 3. 액체발효 후 황금에서 S. aureus 균주에 대하여 9mm 정도의 clear zone이 확인되었고, 단삼과 관중 발효에서는 항균력이 확인되지 않았다. 4. 황금을 L. casei로 고체발효 했을 때 C. albicans와 S. aureus에서 높은 항균활성을 보였다. 단삼은 S. cerevisiae로 고체발효 했을 때 S. aureus에서 항균활성을 보였다. 관중은 L. casei, S. cerevisiae로 고체발효 했을 때 E. coli와 S. aureus에서 항균활성이 높게 나타났다. 발효하지 않았을 때보다 발효 후의 항균활성이 매우 높았다. 5. 유효성분의 변화는 고체발효하지 않은 황금에서는 baicalin 101.57, baicalein 28.26, wogonin 5.33mg/g이었고, S. cerevisiae로 고체발효 했을 때는 baicalin 94.31, baicalein 30.41, wogonin 3.57mg/g로 baicalein의 함량은 증가된 것으로 확인되었다. 발효하지 않은 단삼에서는 salvianolic acid A가 1.82mg/g 이었으며, S. cerevisiae로 고체발효했을 때는 5.70mg/g으로 증가하였다. 관중의 유효성분은 flavaspidic acid AP, flavaspidic acid PB, flavaspidic acid AB, flavaspidic acid BB로 나타났다.

Keywords

Acknowledgement

본 연구는 2022년도 바이오융합소재 상용화 지원사업(R&D) 지원에 의하여 이루어진 것임. 이에 감사드린다.

References

  1. 국가생물종지식정보시스템 www.nature.go.kr/
  2. 김동신. 1998. 식품발효미생물학. 서울:유한문화사.
  3. 도동선. 1993. 충치원생 세균에 대한 관중의 항균활성성분, 충남대학교 대학원, 석사학위.
  4. 이호. 2009. 발효 황금 추출물의 항균작용 및 적용, 충남대학교 대학원, 박사학위.
  5. 이효정. 2008. Methicillin 에 저항력이 있는 Staphylococcus aureus에 대한 관중의 항균활성. 원광대학교대학원 석사학위.
  6. 장소용. 2020. 혈관성 치매 설치류 모델에서 산화질소대사체 함유 마늘발효액, 발효황금 및 홍경천 혼합물이 신경 가소성에 미치는 영향, 원광대학교 대학원 박사학위.
  7. 정연옥, 김용문, 정재한. 2019. 야생 약초 도감, 푸른행복.
  8. Bao-Qing Wang. 2010. Salvia miltiorrhiza: Chemical and pharmacological review of a medicinal plant, Journal of Medicinal Plants Research, vol. 4(25); 2813-2820.
  9. Choi EK, Lee JH, Kim YC, Woo HJ, 2008. Inhibitory Effect of Salvia Miltiorrhiza on Fibrogenesis in Rat Hepatic Stellate Cells, Korean J. Orient. Int. Med., vol.29(2) : 299-310.
  10. Choi HJ, Lee JH, Yun MY, and Lee JS, 2015. Anti-Inflammatory and Whitening Effect of the Lyophilized Powder of Oriental Plant Extracts Fermented with Streptococcus thermophiles, J. Soc. Cosmet. Sci. Korea, vol. 41(2); 159-164. https://doi.org/10.15230/SCSK.2015.41.2.159
  11. Choi WS, Kwon HS, No RH, Choi GP, and Lee HY, 2013. Enhancement of Anti-inflammatory Activities of Fermented Scutellaria baicalensis Extracts using Lactobacillus rhamnosus, J. Soc. Cosmet. Scientists Korea, vol. 39(4); 303-311. https://doi.org/10.15230/SCSK.2013.39.4.303
  12. Earnest Oghenesuvwe Erhirhie, Chika Ndubuisi Emeghebo, Emmanuel Emeka Ilodigwe, Daniel Lotanna Ajaghaku, Blessing Ogechukwu Umeokoli, Peter Maduabuchi Eze, Kenneth Gerald Ngwoke, and Festus Basden Gerald Chiedu Okoye, 2019. Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity Avicenna J Phytomed, vol. 9(4): 396-409.
  13. Han WS, 2004. Isolation of Antimicrobial Compounds from Salvia miltiorrhiza Bunge, Korean J. Medicinal Crop Sci. vol.12(3) : 179-182.
  14. Hwang SH. and Park, C. H., 2009. Preservation of Cosmetics by Ethanol Extract of Scutellaria baicalensis GEORGE. KSBB Journal, vol. 24(4); 347-352.
  15. Im DY, and Lee KL, 2016. Melanin Production Inhibitory Activity of the Dendropanax morbifera Leaf Extract Fermented by Lactobacillus plantarum, Korean J. Pharmacogn., vol. 47(1); 18-23.
  16. Jeon JM, Choi SK, Kim UJ, Jang SJ, Cheon JW, and Lee JS, 2012. Antioxidant and Antiaging Effect of Ginseng Berry Extract Fermented by Lactic Acid Bacteria, J. Soc. Cosmet. Sci. Korea, vol. 37(1); 75-81. https://doi.org/10.15230/SCSK.2011.37.1.075
  17. Jianglin Zhao, Jingfeng Lou, Yan Mou, Peiqin Li, Jianyong Wu and Ligang Zhou, 2011, Diterpenoid Tanshinones and Phenolic Acids from Cultured Hairy Roots of Salvia miltiorrhiza Bunge and Their Antimicrobial Activities, Molecules, vol. 16(3); 2259-2267. https://doi.org/10.3390/molecules16032259
  18. Kang CH, Kim SC, Jeong SC, Han W, Lee SY, Yu SM, Jin HM, and Kim YS, 2016. Physicochemical Characteristics of Fermented Phragmites communis Extract and Its Biological Activity, Kor. J. Pharmacogn., vol. 47(3); 273-279.
  19. Kim HJ, Kim SY, Lee YS and Kim YH, 2014. Determination of Baicalin and Baicalein Contents in Scutellaria baicalensis by NIRS, Korean J. Plant Res. vol. 27(4): 286-292. https://doi.org/10.7732/kjpr.2014.27.4.286
  20. Kim HJ, Lim SW, Choi SW and Yoon CS, 2006. Effect of Methanol Extract of Dryopteris Crassirhizoma in Human Oral Cancer Cells, J. Soc. Cosmet. Scientists Korea vol. 32(3): 201-208.
  21. Kim HY, Choi BH, Hwang HJ, Kim H, Lee KM, Hahm DH, Shim IS, Lee HJ, 2004. Effect of the New Diabetic Formula on Streptozotocin-induced Diabetic Rats, Korean J. Oriental Physiology & Pathology in Medicine, vol. 18(5): 1331-1336.
  22. Kwak YS, Kim MJ, Ahn DJ and Lee JC, 2000. Antimicrobial Activity of Dryopteris rhizoma against Some Food Spoilage Microorganisms, J. Fd Hy. safety vol. 15(1): 36-40.
  23. Lee JS, Lee KS, and Song BK, 2001, Experimental studies on the effect of Samul-Tang and Samul-Tang Gagambang aqua-acupuncture, J. Oriental Obstet. Gynecol., vol. 14(1); 1-26.
  24. Marbun, Tabita Dameria, Song JY, Lee KH, Kim SY, Kang JH, Lee SM, Choi YM, Cho SB, Bae GS, Chang MB, Kim EJ, 2016. Analysis of Antibacterial, Antioxidant, and In Vitro Methane Mitigation Activities of Fermented Scutellaria baicalensis Georgi Extract, Korean J. Org. Agric. vol. 24(4): 735-746. https://doi.org/10.11625/KJOA.2016.24.4.735
  25. Moon KO, and Cha JH, 2020. Enhancement of Antioxidant and Antibacterial Activities of Salvia miltiorrhiza Roots Fermented with Aspergillus oryzae, Foods 2020, vol. 9(1); 34.
  26. Na MK, Jang JP, Min BS, Lee SJ, Lee MS, Kim BY, Oh WK, Ahn JS, 2006. Fatty acid synthase inhibitory activity of acylphloroglucinols isolated from Dryopteris crassirhizoma, Bioorganic & Medicinal Chemistry Letters, vol.16(18); 4738-4742. https://doi.org/10.1016/j.bmcl.2006.07.018
  27. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, and Lee HY, 2006. Effect of Ultrasonification Process on Enhancement of Immuno-stimulatory Activity of Ephedra sinica Stapf and Rubus coreanus Miq., Korean Journal of Biotechnology and Bioengineering, vol. 19(2); 113-117.
  28. Rehn, D., Groenewegen and D., Schenkenberg, D., 1980. Cosmetic preservatives. Part 1, Journal of the Society of Cosmetic Chemists, vol. Soc; 253-267..
  29. Shigeru Abe, Yuichi Sato, Shigeharu Inoue, Hiroko Ishibashi, Naruyama, Toshio Takizawa, Haruyuki Oshima, Hideyo Yamaguchi, 2003. "Anti-Candida albicans Activity of essential Oils Including Lemongrass Oil and its Component", Citral. Jpn J. Med. Myeol., vol. 44: 119-124. https://doi.org/10.3314/jjmm.44.285
  30. Shim HN, Seong KM, Moon SJ, Lee SH, Yang JH, Song BK, 2008. The Effect of the Salvia miltiorrhiza on Axon Regeneration Following Central Nervous System Injury, J. Korean Oriental Med., vol.29(2): 47-59.
  31. Soni MG, Carabin IG, Burdock GA, 2005. "Safety assessment of esters of p-hydroxybenzoic acid (parabens)", Food Chem. Toxcol., vol.43: 985-1015. https://doi.org/10.1016/j.fct.2005.01.020
  32. Um JN, Min JW, Joo KS, and Kang HC, 2017. Enhancement of Antioxidant and Whitening Effect of Fermented Extracts of Scutellariae baicalensis, J. Soc. Cosmet. Sci. Korea vol. 43(3); 201-210. https://doi.org/10.15230/SCSK.2017.43.3.201
  33. Yang HJ, Han HS, Lee YJ, 2013. Effect of Fermented Scutellariae Radix Extract on Production of Inflammatory Mediator in LPS-stimulated Mouse Macrophages, Kor. J. Herbology vol. 28(5); 45-52. https://doi.org/10.6116/KJH.2013.28.5.45
  34. Yang YY, Lee GJ, Yoon DH, Yu Tao, Oh JU, Jeong DO, Lee JS, Kim SH, Kim TW, Cho JY, 2013. ERK1 and TBK1-targeted anti-inflammatory activity of an ethanol extract of Dryopteris crassirhizoma, Journal of Ethnopharmacology, vol. 145(2); 499-508. https://doi.org/10.1016/j.jep.2012.11.019