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Characterization of culturable yeast species associating with whole crop corn and total mixed ration silage

  • Wang, Huili;Hao, Wei;Ning, Tingting;Zheng, Mingli;Xu, Chuncheng
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.2
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    • pp.198-207
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    • 2018
  • Objective: This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods: Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results: In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion: The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic deterioration of TMR silages.

Fauna of Arthropods of Medical Importance in Chindo Island , Korea (전남 진도의 위생 절지동물상)

  • han-II Ree
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.1
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    • pp.87-100
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    • 1995
  • Fauna of medically important arthropods in Chindo Island was studied in July 1994. Among eight speices of mosquitoes (Diptera : Culicidae), Culex tritenirohyunchus was predomiant (46.5% of the total0, and Anopheles sinensis the next. Seven specimens of the unidentified Culex sp. which seemed to be new were also collected. Total 23 species of non-biting midges(Diptera : Chiornomidae) were found in Chindo Is. Of them , Chironomus kiiensis was the predominant species (67.3%) , and Ch.flaviplumus the next (15.6%). Three species, Cladopelma viridula, Dicrotendipes septemmaculatus and Harnischia urtilamellata are reported for the first time in Korea, and they are fully re-described with illustrations. Among five species of biting midges (Diptera : Ceraopogenidae) collected, Culicoides punctatus was predominant (88.7%). A species of Foreipomia was also collected, and this genus is not recorded in Korean fauna. Only two species of domiciliary cockroaches, Periplaneta japonica and P.americana were collected in small numbers and Blatella germanica which is known as the most predominant speices in Korea was not found. The predominant speices of field rodents was Apodemus agrarius and thier ectoparasites were Leptotrombidium orientale (Acarina : Tromicibulidae), Lxodes nipponensis (Acarina : Ixodidae) ; Laelaps jettmani and Eulaelaps stabularis (Acarina : Laelapidae).

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A study of L1 and L2 influences on the speech of Korean-English bilinguals: With special reference to VOT and F0 (한국어-영어 이중 언어 화자들의 L1과 L2 영향에 관한 연구: VOT와 F0와 관련해서)

  • Kim, Mi-Ryoung
    • Phonetics and Speech Sciences
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    • v.7 no.3
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    • pp.13-26
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    • 2015
  • Speech production studies have suggested that bilinguals who are L2-dominant are the most likely to suppress the influence of the first language (L1) on the second language (L2). The voice onset times (VOTs) and fundamental frequencies (f0s) of monolingual and bilingual speakers of English and Korean were examined to address the question whether cross language influences occur particularly in L2 predominant bilinguals and to compare their outcomes with those of L2 proficient bilinguals and monolinguals. A total of 28 speakers participated in this experiment and they produced English and Korean stops in the carrier sentence. In English, for voiceless aspirated and unaspirated stops, L2 predominant bilingual speakers produced VOTs that were significantly shorter than those of monolingual English speakers. The outcome was analogous in Korean speech. For aspirated and lax stops, they produced shorter Korean VOTs than monolingual speakers. The results of f0s were slightly different from those of VOTs. In English, L2 predominant bilinguals produced f0s that were not significantly different from those of monolingual English speakers. In Korean, however, they produced f0s that were significantly different from those of monolingual Korean speakers. Taken VOT and f0 into consideration together, the overall results suggest that, although they tend to show a corresponding pattern of monolinguals, L2 predominant bilinguals had cross language phonetic influences between L1 and L2, similar to L2 proficient bilinguals. Between the two acoustic cues, f0 seemed to be a more reliable cue than VOT to examine the influences.

The clinical study on 44 cases of patient with Thoracolumbar Compression Fracture (흉(胸)·요추(腰椎) 압박골절(壓迫骨折) 환자(患者)에 대한 임상적(臨床的) 고찰(考察))

  • Lim, Jeoung-Eun;Kim, Kee-Hyun;Hwang, Hyeon-Seo
    • Journal of Acupuncture Research
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    • v.17 no.2
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    • pp.41-51
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    • 2000
  • Clinical observation was made on 44 cases of stable thoracolumbar compression fracture that were confirmed through simple x-ray and neurological examination. They were hospitalized and treated by acupuncture and moxibustion, bed rest, herb medicine and physical therapy. If necessary, patient was given an enema. The results obtained are as follows. 1. The patient distribution ratio, in regard to sex, was shown to be 1: 13.7 for males to females. In regard to age, it was shown that people in their 60's was the most predominant case, followed by people in their 70's, 80's, 50's and 40's, respectively. 2. In regard to contributing factors, it was observed that accidental falls were most frequent, followed by reasons unknown, repetitive lifting of heavy objects, overlaboring and bruise in that order. 3. In regard to duration of illness before treatment, it was found that treatment within 1 week was most predominant, followed by over 4 weeks, and 1-2 weeks, respectively. 4. With regard to the duration of hospitalization, hospitalization within 2 weeks was found to be most predominant, followed by 2-4 weeks. 5. In regard to the level of the affected vertebral body; The affected vertebral bodies distributed between T3 to L5 except for T7; T12 was found to be most predominant, followed in turn by L1 and L5. 6. In regard to the number of affected vertebral bodies, 2 was the most frequent followed by 1 and 3, in that order. The average of the number of affected vertebral bodies was observed to be 2.8 and single vertebral body compression fracture was shown to have no clinical and statistical difference as compared with multiple vertebral body compression fractures. 7. In regard to the grade of the seriousness of symptoms, it was found that Grade IV was most predominant, followed by Grade III. 8. With regard to signs at the first medical examination, low back pain was seen in the highest number, and followed in order by gait disturbance, flank pain, flexion-extension disturbance, disturbance of rotation to right or left, and bowel dysfunction. 9. Concerning the effect of treatment, good results were most predominantly seen, and 95.5% of total patients showed fair results. 10. The duration of admission treatment due to the grade of clinical symptoms was as follows; In the case of the Grade IV, it was observed that within 2 weeks was most predominant and for Grade III was 2-4 weeks. It was also found that the grade of clinical symptoms was not in proportion to the duration of admission treatment. 11. In regard to the result of treatment due to the grade of clinical symptoms; It was found that in the case of Grade IV, within 2 weeks was most predominant, and for Grade III, it was found to be 2-4 weeks. 12. Intestinal obstruction was shown in 50% of total patients; In the case of duration of constipation, more than 1 week was found to be most predominant, followed by 2 and 4 days respectively. 13. With regard to the treatment of intestinal obstruction, using acupuncture and moxibustion, herb - medicine and enema together were found to be most effective. 14. Intestinal obstruction was mostly seen in the case of Grade IV.

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Identification of Predominant Bacteria and Fungi in the Industry Treating Soluble Metal Working Fluids (금속가공유 취급 업종에서 우점하는 세균 및 진균의 정성평가)

  • Park, Hae Dong;Park, Dongjin;Park, Hyunhee
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.24 no.4
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    • pp.416-424
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    • 2014
  • Objectives: The objective of this study is to analyze the predominant microorganisms in the industry treating MWFs(Metal working fluids). Methods: The bacteria and fungi were collected by agar plate impaction and bulk MWFs in storage tank at 54 sites in 9 shops in South Korea. The dominant bacteria and fungi isolated from agar media were identified by fatty acid analysis and morphological analysis, respectively. Results: Totally 111 dominant bacteria were identified in the process, outdoor, and bulk MWFs. The predominant bacterial genus was Micrococcus and Bacillus in the process and outdoor, Pseudomonas in bulk MWF. Among the identified 119 strains of fungi, Cladosporium and Penicillium genus were dominated. The ratios of bacteria designated biosafety level 2 and 1 were 30% and 21%, respectively. Conclusions: This study has investigated the dominant microorganisms in soluble MWF using industry. And it was useful that the qualitative evaluation method along with quantitative analysis for better understanding of the biological factors in the work environment.

Human Papillomavirus Genotypes Associated with Mucopurulent Cervicitis and Cervical Cancer in Hangzhou, China

  • Shen, Xing-Hang;Liu, Shu-Hua
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3603-3606
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    • 2013
  • Background: To investigate the infection status and predominant genotype distribution of human papillomavirus (HPV) infection among Chinese patients with mucopurulent cervicitis (MPC) or cervical cancer (CC) in Hangzhou. Methods: Initially, 217 cases of healthy cervix controls (n=50), acute MPC (n=89), and CC (n=78) were included; samples were collected between January 1, 2010, and January 30, 2013. Cervical specimens were screened for HPV using a nested polymerase chain reaction assay and DNA sequencing. Results: Overall prevalence of HPV infection was 16.7% in the control group, 51.9% in the MPC group, and 84.4% in the CC group. The predominant genotype detected in all 3 groups was the oncogenic variant HPV 16 (55.8%, 17.3%, and 6.3% in the CC, MPC and control specimens, respectively), HPV58 was the second most predominant HPV type in CC (9.1%), MPC (8.6%), and control group (4.2%). Most or all of the genotypes were oncogenic in the three groups. Conclusions: Infection with HPV was found to be prevalent among Chinese women with MPC or CC and oncogenic variants were in the majority. Therefore, peoples who suffered MPC with HPV DNA positive should be regularly followed-up, for prevention and early treatment of cervical cancer.

Identification and Distribution of predominant tactic Acid Bacteria in Kimchi, a Korean Traditional Fermented Food

  • Kim, Tae-Woon;Lee, Ji-Yeon;Jung, Sang-Hoon;Kim, Young-Mok;Jo, Jae-Sun;Chung, Dae-Kyun;Lee, Hyong-Joo;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.635-642
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    • 2002
  • To effectively investigate the identification and distribution of the lactic acid bacteria in Kimchi, polyphasic methods, including a PCR, SDS-PAGE of the whole-cell proteins, and 16S rRNA gene sequence analysis, were used. In various types of Kimchi fermented at 20$\^{C}$, the isolate KHU-31 was found to be the predominant lactic acid bacteria. This isolate was identified as Lactobacillus sake KHU-31, based on SDS-PAGE of the whole-cell proteins and a 165 rRNA gene sequence analysis, which provided accurate and specific results. Accordingly, the approach used in the current study demonstrated that Lactobacillus sake KHU-31, together with Leuconostoc mesenteroides, were the most predominant lactic acid bacteria in all types of Kimchi in the middle stage of fermentation at 20$\^{C}$.

Effect of Nutrient Composition and Air Regulation on Bulking in the Activated Sludge Process (활성오니공법에서 영양염류 조성비와 공기조절이 팽화발생에 미치는 영향)

  • 이장훈;권혁구;강병곤;정준오
    • Journal of Environmental Health Sciences
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    • v.30 no.1
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    • pp.7-14
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    • 2004
  • The change in filamentous bacteria appearance was observed by adjusting factors such as organic and nurient concentration of feed wastewater and the aeration rate in the reactor. In case that COD:N:P and DO were 100:10:1 and 6.1 m1/1 respectively, the mean SVI was 122 ml/g and the filaments were developed normally in flocs. For the low DO con- dition, however, the SVI averaged 186 ml/g and the appearance of outgrowing filaments were more frequent. When the high organic was supplied into the reactor, the average SVI was 274 ml/g and the distinct filamentous bulking was observed. Meanwhile when COD:N:P was maintained at 100: 1 :0.5, the SVI was as low as 87 ml/g and the appearance of filaments were minimal and the size of flocs was small comparing other experimental conditions. For normal, low aeration and high organic concentration, predominant filaments in the early stage of experiments were commonly Type 021N,S. natans which were usually found in low DO condition. However, Type 041, Type 1851, Type 0961 became predominant as experiments extended. Meanwhile, in low nutrient condition, Type 0675, Type 1851, and Type 0961 were observed. The filamentous bacteria appearance for SVI< 150(ml/g), Type 0041, Type 0961 (usually low organic in feed wastewater) were predominantly observed and SVI > 150(ml/g), S. natans and Type 021N(usually low DO in aeration basin) was predominant.

An immunohistochemical study of endocrine cells in the alimentary tract of the snake, Rhabdophis tigrinus tigrinus

  • Lee, Jae-hyun;Ku, Sae-kwang;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.39 no.4
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    • pp.689-697
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    • 1999
  • The regional distribution and relative frequency of endocrine cells in the alimentary tract of the snake, Rhabdophis tigrinus tigrinus, were investigated by immunohistochemical method using 7 antisera. Chromogranin (Cg)-, glucagon-, somatostatin-, gastrin/cholecystokinin (Gas/CCK)-, serotonin-, bovine pancreatic polypeptide (BPP)-immunoreactive cells were identified in this study. Cg-immunoreactive cells were detected throughout the alimentary tract including the esophagus, with predominant frequency in the pylorus. Numerous immunoreactive cells were observed from the esophagus to the pylorus but a few cells were detected in the large intestine. Glucagon-immunoreactive cells were observed from the proximal portions to the distal portions of the small intestine. They were increased to the middle portions but thereafter decreased, and no cells were found in the terminal portions. Somatostatin-immunoreactive cells were restricted to the small intestine and these cells were decreased toward to distal portions of the small intestine. Gas/CCK-immunoreactive cells were detected in the pylorus and small intestine. They were most predominant in the pylorus and the proximal portions of the small intestine but thereafter decreased toward to the distal regions. Serotonin-immunoreactive cells were observed throughout the alimentary tract. They were most predominant in the pylorus and proximal portions of the small intestine but a few cells were observed in the large intestine. BPP-immunoreactive cells were restricted to the distal portions of the small intestine with rare frequency. No bombesin-immunoreactive cells were found in this study.

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Effect of Casing Layer on Growth Promotion of the Edible Mushroom Pleurotus ostreatus

  • Cho, Young-Sub;Weon, Hang-Yeon;Joh, Jung-Ho;Lim, Jong-Hyun;Kim, Kyung-Yun;Son, Eun-Suk;Lee, Chang-Soo;Cho, Bong-Gum
    • Mycobiology
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    • v.36 no.1
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    • pp.40-44
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    • 2008
  • Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.