• BMB Reports
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• 제39권6호
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• pp.671-676
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• 2006

The holoenzyme of protein phosphatase (PP) from tulip petals was purified by using hydrophobic interaction, anion exchange and microcystin affinity chromatography to analyze activity towards p-nitrophenyl phosphate (p-NPP). The catalytic subunit of PP was released from its endogenous regulatory subunits by ethanol precipitation and further purified. Both preparations were characterized by immunological and biochemical approaches to be PP2A. On SDS-PAGE, the final purified holoenzyme preparation showed three protein bands estimated at 38, 65, and 75 kDa while the free catalytic subunit preparation showed only the 38 kDa protein. In both preparations, the 38 kDa protein was identified immunologically as the catalytic subunit of PP2A by using a monoclonal antibody against the PP2A catalytic subunit. The final 623- and 748-fold purified holoenzyme and the free catalytic preparations, respectively, exhibited high sensitivity to inhibition by 1 nM okadaic acid when activity was measured with p-NPP. The holoenzyme displayed higher stimulation in the presence of ammonium sulfate than the free catalytic subunit did by protamine, thereby suggesting different enzymatic behaviors.

• Mass Spectrometry Letters
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• 제11권1호
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• pp.10-14
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• 2020

Riboflavin is a water-soluble vitamin, which serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide. This study aimed to develop a simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the quantification of riboflavin in the Beagle dog plasma. This method utilized simple protein precipitation with acetonitrile and ¹³C₄, ¹⁵N₂-riboflavin was used as an internal standard (IS). For chromatographic separation, a hydrophilic interaction liquid chromatography (HILIC) column was used with gradient elution. The mobile phase consisted of 0.1% (v/v) aqueous formic acid with 10 mM ammonium formate and acetonitrile with 0.1% (v/v) formic acid. Since riboflavin is an endogenous compound, 4% bovine serum albumin in phosphate buffered saline was used as a surrogate matrix to prepare the calibration curve. The quantification limit for riboflavin in the Beagle dog plasma was 5 ng/mL. The method was fully validated for its specificity, sensitivity, accuracy and precision, recovery, and stability according to the US FDA guidance. The developed LC-MS/MS method may be useful for the in vivo pharmacokinetic studies of riboflavin.

• The Korean Journal of Ceramics
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• 제5권2호
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• pp.125-130
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• 1999

After $SnO_2$ fine powder by precipitation method, Ca as crystallization inhibitor and Pd as catalyst were added to $SnO_2$ raw material by various methods. Thick film device was fabricated on the alumina substrate by mixing ethylene glycol and such mixed powders. The sensing characteristics of the device for methane gas were investigated. The most excellent gas sensing property was shown by the thick film device fabricated by Method 3 in which Ca and Pd doped $SnO_2$ powder is prepared by mixing $SnO_2$ powder, 0.1 wt% Ca acetate and 1 wt% $PdCl_2$ in deionized water and by calcining the mixture, after $Sn(OH)_4$ is dried at $110^{\circ}C$ for 36h. The sensitivity of the sensor fabricated with $SnO_2$-0.1 wt%Ca acetate-1wt%$PdCl_2$ powder heat-treated at $700^{\circ}C$ for 1h was about 86% for 5,000 ppm methane in air at $350^{\circ}C$ of the operating temperature. Response time and recovery were also excellent.

• Journal of Electrical Engineering and Technology
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• 제13권4호
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• pp.1697-1704
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• 2018

As a key component of lighting location system (LLS) for lightning warning, the atmospheric electric field measuring is required to have high accuracy. The Conventional methods of the existent electric field measurement meter can only detect the vertical component of the atmospheric electric field, which cannot acquire the realistic electric field in the thunderstorm. This paper proposed a three dimensional (3D) electric field system for atmospheric electric field measurement, which is capable of three orthogonal directions in X, Y, Z, measuring. By analyzing the relationship between the electric field and the relative permittivity of ground surface, the permittivity is calculated, and an efficiency 3D measurement model is derived. On this basis, a three-dimensional electric field sensor and a permittivity sensor are adopted to detect the spatial electric field. Moreover, the elevation and azimuth of the detected target are calculated, which reveal the location information of the target. Experimental results show that the proposed 3D electric field meter has satisfactory sensitivity to the three components of electric field. Additionally, several observation results in the fair and thunderstorm weather have been presented.

• Mass Spectrometry Letters
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• 제10권2호
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• pp.56-60
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• 2019

Coptidis Rhizoma (CR) has been used widely in traditional medicine to treat common diseases. This study aimed to develop a high-sensitivity liquid chromatography-tandem mass (LC-MS) spectrometry method for the evaluation of the pharmacokinetics of a new natural product that contain CR extract with the main bioactive compound, berberine, at trace concentrations. Human plasma samples were pretreated with methanol by a protein precipitation method. Berberine was analyzed on a Kinetex C18 column ($2.1mm{\times}50mm$, $100{\AA}$, $1.7{\mu}m$) using a mobile phase of 10 mM ammonium formate/0.1% formic acid in water (A) and acetonitrile (B) (50:50, v/v) with a flow rate of 0.25 mL/min. The analyte was detected by using electrospray ionization in positive mode with multiple reaction monitoring (MRM). The method was sensitive, with a lower limit of quantification of 1 pg/mL, which has not been previously obtained. The method was validated (over the range of 1-50 pg/mL) and applied successfully for the pharmacokinetic study of human plasma samples.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.112-117
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• 2021

α-Amanitin and β-amanitin are highly toxic bicyclic octapeptides responsible for the poisoning of poisonous mushrooms such as Amanita, Galerina, and Lepiota by inhibiting RNA polymerase II, DNA transcription, and protein synthesis. A sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using parallel reaction monitoring mode was developed and validated for the simultaneous determination of α- and β-amanitin in mouse plasma to evaluate the toxicokinetics of α- and β-amanitin in mice. Protein precipitation of 5 μL mouse plasma sample with methanol as sample clean-up procedure and use of negative electrospray ionization resulted in better sensitivity and less matrix effect. The calibration curves for α- and β-amanitin in mouse plasma were linear over the range of 0.5-500 ng/mL. The intra- and inter-day coefficient of variations and accuracies for α- and β-amanitin at four quality control concentrations were 3.1-14.6% and 92.5-115.0%, respectively. The present method was successfully applied to the toxicokinetic study of α- and β-amanitin after an oral administration of α- and β-amanitin at 1.5 mg/kg dose to male ICR mice.

• 대기
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• 제32권4호
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• pp.263-276
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• 2022

The observation error of satellite radiation data that assimilated into the Korean Integrated Model (KIM) was diagnosed by applying the Hollingsworth and Lönnberg and Desrozier techniques commonly used. The magnitude and correlation of the observation error, and the degree of contribution for the satellite radiance data were calculated. The observation errors of the similar device, such as Advanced Technology Microwave Sounder (ATMS) and Advanced Microwave Sounding Unit-A shows different characteristics. The model resolution accounts for only 1% of the observation error, and seasonal variation is not significant factor, either. The observation error used in the KIM is amplified by 3-8 times compared to the diagnosed value or standard deviation of first-guess departures. The new inflation value was calculated based on the correlation between channels and the ratio of background error and observation error. As a result of performing the model sensitivity evaluation by applying the newly inflated observation error of ATMS, the error of temperature and water vapor analysis field were decreased. And temperature and water vapor forecast field have been significantly improved, so the accuracy of precipitation prediction has also been increased by 1.7% on average in Asia especially.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.147-147
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• 2022

In January 2021 heavy flood affected South Kalimantan with causing many casualties. The heavy rainfall is predicted to be generated due to the ENSO (El Nino-Southern Oscillation). The weak La-Nina mode appeared to generate more convective cloud above the warmed ocean and result in extreme rainfall with high anomaly compared to past historical rainfall event. Subsequently, the antecedent soil moisture distribution showed to have an important role in generating the flood response. Saturated flow and infiltration excess mainly contributed to the runoff generation due to the high moisture capacity. The hydro-meteorological processes in this event were deeply analyzed using the coupled atmospheric model of Weather Research and Forecasting (WRF) and the hydrological model extension (WRF-Hydro). The sensitivity analysis of the flood response to the SST anomaly and the soil moisture capacity also compared. Result showed that although SST and soil moisture are the main contributors, soil moisture have more significant contribution to the runoff generation despite of anomaly rainfall occurred. Model performance was validated using the Global Precipitation Measurement (GPM) and Soil Moisture Operational Products System (SMOPS) and performed reasonably well. The model was able to capture the hydro-meteorological process of atmosphere and hydrological feedbacks in the extreme weather event.

• Asian Journal of Business Environment
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• 제14권3호
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• pp.1-11
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• 2024

Purpose: This study analyzes the impact of weather conditions, holidays, and sporting events on beer sales, providing insights for market strategy and inventory management in the beer industry. Research design, data and methodology: Beer types were classified into Lagers and Ales, with further subcategories. The study utilized weekly retail sales data from January 2018 to August 2020, provided by Nielsen Korea. An ARMAX model was employed for time-series analysis. Results: The analysis revealed that increasing temperatures positively influence sales of Pilsners and Pale Lagers. Conversely, higher precipitation levels negatively affect overall Lager sales. Among Ales, only Stout sales showed a significant decrease with increased rainfall. Sunshine duration did not significantly impact sales for any beer type. Humidity generally had little effect on beer sales, with the exception of Amber Lagers, which showed sensitivity to humidity changes. Holidays and sporting events were found to significantly boost sales across most beer types, although the specific impacts varied by beer category. Conclusions: This study offers a detailed analysis of how weather conditions and specific events influence different beer type sales. The findings provide valuable insights for breweries, beer processors, and retailers to optimize their market strategies and inventory management based on weather forecasts and seasonal events. By understanding the consumption patterns of each beer type in relation to environmental factors, businesses can better anticipate demand fluctuations and tailor their operations accordingly.

• 대한지리학회지
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• 제43권5호
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• pp.681-700
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• 2008

본 연구에서는 우리나라 61개 지점 기온 및 강수 자료에서 산출한 31개의 극한 기후 지수를 바탕으로 최근 35년 동안(1973-2007)의 계절 평균 값의 변화에 따른 우리나라 극한 기온 및 강수 사상의 시 공간적 변화 패턴을 조사하였다. 연구기간 동안 우리나라 평균적으로 기상학적 겨울철(12-2월) 일 최고기온(일 최저기온)은 $+0.60^{\circ}C$/10년($+0.54^{\circ}C$/10년)의 비율로 상승한 반면, 여름철(6-8월) 극한 기온 평균은 통계적으로 유의미한 변화를 나타내지 않았다. 기온 극한 사상의 경우에는 연구기간 동안 상 하위 10번째 퍼센타일 값을 기준으로 한 우리나라 평균 한랭한 밤과 낮의 발생빈도가 각각 -9.2 일/10년과 -3.3 일/10년의 감소추세를 보인 반면, 온난한 밤과 낮의 발생빈도는 각각 +4.9 일/10년과 +6.8 일/10년의 증가추세를 보였다. 이와 같은 극한 기온 사상의 변화는 계절적으로 겨울철에 더 크게 나타났지만, 오히려 일 최저 또는 일 최고 평균 상승에 따른 여름철 무더운 밤(+8.0 일/$^{\circ}C$)과 낮(16.6 일/$^{\circ}C$)의 증가율은 겨울철 추운 밤(-5.2 일/$^{\circ}C$)과 낮(-4.3 일/$^{\circ}C$)의 감소율보다 더 높게 나타났다. 이러한 패턴은 우리나라 기온 극한 사상의 변화가 주야간 그리고 계절별로 비대칭적으로 나타나고 있다는 것을 가리킨다. 한편 우리나라 평균적인 연 총 강수량은 연구기간 동안 85.5mm/10년의 비율로 증가추세를 보였는데, 특히 $7{\sim}8$월 강수량이 통계적으로 유의미하게 증가하여 여름 장마 이후의 이중 극대점의 강수분포가 다중 극대점 구조로 전환되었다. 이러한 여름 강수패턴의 변화는 주로 40mm 이상의 여름철 극한 강수일이 증가하면서 나타났고, 공간적으로 통계적으로 유의미한 극한 강수일의 증가 추세는 태백산맥 주변 지역을 중심으로 나타났다.