• Structural Engineering and Mechanics
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• 제63권1호
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• pp.23-35
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• 2017

A nonlinear static procedure considering work-energy principle and global failure mechanism to estimate base shears of reinforced concrete (RC) frame-type structures is presented. The relative energy equation comprising of elastic vibrational energy, plastic strain energy and seismic input energy is obtained. The input energy is modified with a factor depending on damping ratio and ductility, and the energy that contributes to damage is obtained. The plastic energy is decreased with a factor to consider the reduced hysteretic behavior of RC members. Given the pre-selected failure mechanism, the modified energy balance equality is written using various approximations for modification factors of input energy and plastic energy in scientific literature. External work done by the design lateral forces distributed to story levels in accordance with Turkish Seismic Design Code is calculated considering the target plastic drift. Equating the plastic energy obtained from energy balance to external work done by the equivalent inertia forces considering, a total of 16 energy-based base shears for each frame are derived considering different combinations of modification factors. Ductility related parameters of modification factors are determined from pushover analysis. Relative input energy of multi degree of freedom (MDOF) system is approximated by using the modal-energy-decomposition approach. Energy-based design base shears are compared with those obtained from nonlinear time history (NLTH) analysis using recorded accelerograms. It is found that some of the energy-based base shears are in reasonable agreement with the mean base shear obtained from NLTH analysis.

• 한국방재학회 논문집
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• 제4권2호
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• pp.35-45
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• 2004

최근 항공기, 자동차 등에 사용되어왔던 복합재료가 그 사용 영역을 넓혀 교량 상판, 상하수도용 관로 등에 적용되고 있다. 이러한 복합재료의 주요 장점은 고강도, 경량성, 그리고 뛰어난 내부식성으로 기존의 재료에 비해 많은 장점을 갖고 있으나, 이러한 재료의 정확한 특성 파악 및 이를 사용한 구족물의 거동의 정확한 분석의 어려움으로 복합재료의 적용이 활발히 이루어지지 못하고 있는 실정이며, 이러한 요인이 복합재료를 토목구조물에 적용하는데 가장 큰 제한요소로 작용해 왔다. 본 연구에서는 복합재료 구조물의 해석을 위한 사전 연구로서, 현재 국내에서 사용되고 있는 GFRP 관로에 대한 재료특성 실험을 수행하여, 각각의 구성 적층재료별 재료 특성 및 전체 적층판에 대한 재료특성을 제공한다.

• 한국산학기술학회논문지
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• 제13권3호
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• pp.1396-1404
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• 2012

복합말뚝은 서로 다른 재질을 사용하여 말뚝을 구성하는 말뚝으로 상부는 강관말뚝 하부는 콘크리트 말뚝으로 구성된 말뚝을 선 굴착 공법으로 시공하였다. 말뚝에는 7쌍의 변형률계와 경사계를 설치하여 말뚝의 수평재하시험 중 말뚝에 작용하는 응력과 변위를 측정하였다. 측정된 계측치는 여러 가지 이론적 공식을 이용하여 분석 비교하는 역 해석을 수행하였다. 분석결과 공식들로부터 예측한 말뚝에 작용하는 응력은 실제 측정된 값보다 크게 평가 되었으며, 말뚝상부에 응력이 크게 측정되다가 말뚝깊이에 따라 급격하게 감소하는 경향을 보였다. 측정된 응력은 모두 말뚝재질의 응력을 초과 하지 않아 복합말뚝의 설계개념을 검증할 수 있었다.

• Geomechanics and Engineering
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• 제14권5호
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• pp.459-466
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• 2018

By conducting uniaxial loading cycle tests on the coal rock with outburst proneness, the dilatation characteristics at different loading rates were investigated. Under uniaxial loading and unloading, the lateral deformation of coal rock increased obviously before failure, leading to coal dilatation. Moreover, the post-unloading recovery of the lateral deformation was rather small, suggesting the onset of an accelerated failure. As the loading rate increased further, the ratio of the stress at the dilatation critical point to peak-intensity increased gradually, and the pre-peak volumetric deformation decreased with more severe post-peak damage. Based on the laboratory test results, the lateral deformation of the coals at different depths in the #1302 isolated coal pillars, Yangcheng Coal Mine, was monitored using wall rock displacement meter. The field monitoring result indicates that the coal lateral displacement went through various distinct stages: the lateral displacement of the coals at the depth of 2-6 m went through an "initial increase-stabilize-step up-plateau" series. When the coal wall of the working face was 24-18 m away from the measuring point, the coals in this region entered the accelerated failure stage; as the working face continued advancing, the lateral displacement of the coals at the depth over 6 m increased steadily, i.e., the coals in this region were in the stable failure stage.

• Geomechanics and Engineering
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• 제20권4호
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• pp.359-370
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• 2020

Prestressed ground anchors are important structural elements in geotechnical engineering. Despite their widespread usage, the design process is often significantly simplified. One of the major drawbacks of commonly used design methods is the assumption that skin friction is mobilized uniformly along an anchor's fixed length, one consequence of which is that a progressive failure phenomenon is neglected. The following paper introduces an alternative design approach - a computer algorithm employing the load-transfer method. The method is modified for the analysis of anchors and combined with a procedure for the derivation of load-transfer functions based on commonly available laboratory tests. The load-transfer function is divided into a pre-failure (hardening) and a post-failure (softening) segment. In this way, an aspect of non-linear stress-strain soil behavior is incorporated into the algorithm. The influence of post-grouting in terms of radial stress update, diameter enlargement, and grout consolidation is included. The axial stiffness of the anchor body is not held constant. Instead, it gradually decreases as a direct consequence of tensile cracks spreading in the grout material. An analysis of the program's operation is performed via a series of parametric studies in which the influence of governing parameters is investigated. Finally, two case studies concerning three investigation anchor load tests are presented.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.1082-1091
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• 2020

Microbial transglutaminases (MTGs) are widely used in the food industry. In this study, the MTG gene of Streptomyces sp. TYQ1024 was cloned and expressed in a food-grade bacterial strain, Bacillus subtilis SCK6. Extracellular activity of the MTG after codon and signal peptide (SP Ync M) optimization was 20 times that of the pre-optimized enzyme. After purification, the molecular weight of the MTG was 38 kDa and the specific activity was 63.75 U/mg. The optimal temperature and pH for the recombinant MTG activity were 50℃ and 8.0, respectively. MTG activity increased 1.42-fold in the presence of β-ME and 1.6-fold in the presence of DTT. Moreover, 18% sodium chloride still resulted in 83% enzyme activity, which showed good salt tolerance. Cross-linking gelatin with the MTG increased the strength of gelatin 1.67 times and increased the thermal denaturation temperature from 61.8 to 75.8℃. The MTG also significantly increased the strength and thermal stability of gelatin. These characteristics demonstrated the huge commercial potential of MTG, such as for applications in salted protein foods.

• 한국미생물·생명공학회지
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• 제30권4호
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• pp.332-338
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• 2002

토양으로부터 세포외로 $\alpha$-galactosidase를 분비 생산하는 방선균 YB-4가 분리되었으며, 분리균의 배양ㆍ형태ㆍ생리적 특성을 조사한 결과 Streptomyces 속 균주로 확인되었다. 분리균의 배양상등액으로부터 부분정제된 $\alpha$-galactosidase를 조효소액으로 사용하였을 때 para-nitrophenyl-$\alpha$-D-galacto-pyranoside는 pH 6.0과 6$0^{\circ}C$의 반응조건에서 가장 잘 분해되었으며, 조효소액을 pH 4.0에서 pH 10.0범위에서 1시간 이상 방치한 후에도 약 90% 이상의 $\alpha$-galactosidase 활성을 유지하였다. 또한 분리균이 생산하는 $\alpha$-galactosidase는 melibiose, raffinose와 stachyose와 같은 저당류를 가수분해 할 수 있으며 분해산물로 galactose를 방출하는 것으로 보아 $\alpha$-1,6 결합을 분해한 것으로 확인되었다.

• Mycobiology
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• 제38권4호
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• pp.286-294
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• 2010

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.

• 펄프종이기술
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• 제42권4호
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• pp.39-44
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• 2010

To increase the fracture toughness of paper made of Sw-BKP, refining and kneading conditions were analyzed. Curl and kink was known to increase the strain at break and the fracture touhness. Sequence of multiple stage beating, beating load and kneading were compared. When we applied a kneading treatment using a kneader at the final step, the most of the fiber transformation such as curl and kink occurred, the more the bulk and air-permeability improved. Physical strength and TEA(tensile energy absorption) were increased higher when kneading treatment before refining than only refining treatment was performed. TEA was increased because of higher elongation. It was found that the highest fracture toughness was obtained when applying the kneading treatment to the fibers in the pre-treatment step rather than in the middle step of beating or in the final step of beating.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 춘계학술대회 논문집
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• pp.114-117
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• 2004

The isothermal forging design of a Ti-6Al-4V wing shape was performed by 3D FE simulation. The design focuses on near-net shape forming by the single stage. The process variables such as the die design, pre-form shape and size, ram speed and forging temperature were investigated. The minimization of forging load and uniform strain distribution in a given forging condition were considered as main design factors. The FE simulation results fur the final process design were compared with the isothermal forging tests. Finally, the modified process design for producing the uniform Ti-6Al-4V wing product without forming defects was suggested.