• KSBB Journal
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• v.26 no.5
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• pp.427-432
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• 2011

In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.

• Structural Engineering and Mechanics
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• v.58 no.2
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• pp.347-378
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• 2016

Within the scope of the plane-strain state, by utilizing the three-dimensional linearized theory of elastic waves in initially stressed piezoelectric and elastic materials, Lamb wave propagation and the influence of the initial stresses on this propagation in a sandwich plate with pre-stressed piezoelectric face and pre-stressed metal elastic core layers are investigated. Dispersion equations are derived for the extensional and flexural Lamb waves and, as a result of numerical solution to these equations, the corresponding dispersion curves for the first (fundamental) and second modes are constructed. Concrete numerical results are obtained for the cases where the face layers' materials are PZT-2 or PZT-6B, but the material of the middle layer is Steel (St) or Aluminum (Al). Sandwich plates PZT-2/St/PZT-2, PZT-2/Al/PZT-2, PZT-6B/St/PZT-6B and PZT-6B/Al/PZT-6B are examined and the influence of the problem parameters such as piezoelectric and dielectric constants, layer thickness ratios and third order elastic constants of the St and Al on the effects of the initial stresses on the wave propagation velocity is studied.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.6
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• pp.141-147
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• 1999

Optical disk technology with a laser beam for data recording and retrieval is one of the most promising route for high density information storage in multimedia era. As the storage density and data transfer rates are increased, mechanical issues, mainly noise and vibration, become critical. Rubber materials are extensively used in various machine design application, mainly for vibration/shock/noise control devices. Over the years an enormous effort has been put into developing procedures to provide properties of rubber components with complex shape and under pre-deformed state. In this paper, non-linear large deformations of a rubber mount for optical disk drive were investigated using the finite element method. A tension test of rubber material was performed, to calculate a strain energy function. According to the pre-deformed state, the variation of rubber mount stiffness were calculated and the reliability of numerical results were checked by compared with the measuring the deflection values. Also, the effects of the pre-deformed rubber mount on the system dynamic characteristics were investigated and the relation between the static stiffness variation of rubber mount and the natural frequence variation of system was discussed.

• Journal of Environmental Science International
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• v.28 no.11
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• pp.927-933
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• 2019

A lipid-enriched strain of Botryococcus braunii (UTEX 572) was cultivated in a semi-batch aeration tank to enhance biomass as well as to develop intracellular lipids and fatty acids. A 30 day period of incubation produced 1.39 g/L of biomass and 0.31 g/L of total lipids in the biomass. The grown biomass was pre-treated using several methods to extract the total lipid content efficiently: ultrasonication was found to yield the highest percentage of lipids-namely 19.8% per biomass. Direct heating of biomass in an autoclave also showed better performance than when using only conventional solvent extraction. To enhance the biomass harvest and lipid extraction efficiency, coagulation and flocculation steps were added to the extraction process. It is noteworthy that not only the solvent type but also the solvent/biomass ratio greatly affected efficiency. In addition, the moisture content of the harvested(wet) biomass affected the efficiency significantly. This study elucidated the need for future research on optimizing this extraction process.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.429-444
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• 1998

Objective of this study was to select ectomycorrhizal fungi for black spruce(Picea mariana) inoculation to overcome the growth inhibitory effects of Kalmia angustifolia. Nineteen isolates representing 11 species of ectomycorrhizal fungi were tested for their abilities to grow and form mycorrhizae with black spruce seedlings in the presence of water leachate of leaves of Kalmia. Mycelium growth of 9 isolates were inhibited by the leaf leachate. Colony diameter and biomass of the other 10 isolates were either increased or unaffected under the same conditions. Acidic pH of the culture medium(pH 3 and 4) inhibited some of the fungi, but a combination of acidic pH and the leaf leachate was more inhibitory. Thirteen isolates were able to form ectomycorrhizae with black spruce in presence of 25% leaf leachate in pure culture. Four isolates, Paxillus involutus(NF4), Cenococcum geophilum(GB12), Laccaria laccata(GB23), and E-strain(GB45) formed mycorrhizae more successfully than the others in presence of up to 50% Kalmia leaf leachate. Black spruce seedlings pre-inoculated with these fungi were grown with Kalmia leaf leachate and live Kalmia plants during a four month greenhouse experiment. Abundant mycorrhizae(77-91% of root tips) were developed on seedlings pre-inoculated with P. involutus, L. laccata and E-strain but relatively poor mycorrhization(32% of root tips) resulted with C. geophidum. Over 90% of the short root mycorrhizae were attributed to the inoculated fungi although indigenous mycorrhizae also occurred on most seedlings. Persistence of the mycorrhizae was not affected by living Kalmia plants. Over 80% of the mycorrhizae on seedlings inoculated with P. involutus, L. laccata and E-strain and 53% of the mycorrhizae on seedlings inoculated with C. geophilum were attributable to the inoculant fungi. Control seedlings formed about 45% ectomycorrhizal short roots with indigenous fungi. The L. laccata and C. geophilum inoculated seedlings exhibited enhanced mycorrhizae formation in presence of Kalmia leaf leachate. Mycorrhizae formation with inoculant fungi was 4-15% lower at pH 4 than at pH 5, with the greatest inhibition occurring for L. laccata. Seedlings inoculated with P. involutus had the greatest shoot and root growth followed by L. laccata and E-strain inoculated seedlings. The P. involutus and L. laccata inoculated seedlings were significantly taller with more shoot dry biomass than the uninoculated(control) seedlings. E-strain inoculated seedlings had significantly higher shoot dry biomass and significantly lower number of first order lateral roots compared to the control but other growth parameters such as height, root dry weight and number of short root tips were not significantly different from the control. Seedlings inoculated with C. geophilum were not significantly different from the uninoculated seedlings in any of the growth parameters except for the number of first artier lateral roots which was significantly less than the control seedlings.

• Journal of Welding and Joining
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• v.34 no.1
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• pp.59-67
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• 2016

The automotive manufactures increase their use of lightweight materials to improve fuel economy and energy usage has a significant influence on the choice of developing materials. To meet this requirements manufacturers are replacing individual body parts with lightweight metals, for these the process treating and painting surfaces is changing. The pre-coated steels are newly developed to avoid the conventional complex and non-environmental painting process in the body-in-white car manufacturing. The development of new joining techniques is critically needed for pre-coated steel sheets, which are electrically non-conductive materials. In the present study, dissimilar combination of pre-coated steel and galvanized steel sheets were joined by the self-piercing rivet, adhesive bonding and hybrid joining techniques. The tensile shear test and free falling high speed crash test were conducted to evaluate the mechanical properties of the joints. The highest tensile peak load with large deformation was observed for the hybrid joining process which has attained 48% higher than the self-piercing rivet. Moreover, the hybrid and adhesive joints were observed better strain energy compared to self-piercing rivet. The fractography analyses were revealed that the mixed mode of cohesive and interfacial fracture for both the hybrid and adhesive bonding joints.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.10a
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• pp.71-74
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• 2005

In manufacturing automotive parts, such as engine cradles, frame rails, subframes, cross-members, and other parts from circular tubes, pre-bending and pre-forming operations are often required prior to the subsequent tubular hydroforming process. During some pre-forming operations, the cross section of a bent circular tube is crushed into an oval-like shape to ensure proper geometry and sufficient clearance in the hydroforming dies. For such applications, the use of oval Instead of circular tubes could be an effective means of eliminating the pre-forming step. The oval tube could also be produced with less thinning and with less strain on the outside of the bend when controlled by a booster system without the use of mandrel. Hence, the understanding of the issues that occur in the bending of oval tubes is worthy of Investigation. This paper presents parametric studies on the bending of oval tubes without a mandrel. The finite element modeling technique is used to examine the deformation characteristics for both circular and oval tubes. In the simulations, the bending process parameters of bend radius, aspect ratio of the tube ovalness, and tube wall thickness are varied. Observations are made to obtain a hoop-buckle limit diagram in terms of a non-dimensional shape degradation factor. Suggestions based upon developed criteria are made on the acceptability of bend tubes suitable for hydroforming applications without the need ofa pre-forming step or the used of a mandrel.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
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• 2004.05a
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• pp.263-267
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• 2004

This study presents the combined effect of electric field application and mechanical compressive stress loading on deformation in a multilayer ceramic actuator, designed with stacking 0.2($PbMn_{1/3}Nb_{2/3}O_{3}$)-0.8($PbZr_{0.475}Ti_{0.525}O_{3}$) ceramics and Ag-Pd electrode, alternately. The deformation behaviors were attributed to relative $180^{\circ}$domain, determined by pre-loaded compressive stress and electric field. The non-linearity of piezoelectricity and strain are dependent upon the young's modulus resulting from the domain reorientation.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.5
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• pp.79-86
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• 2009

With the substantial increase of the size of structure, the management of excavation becomes more difficult. Therefore, massive collapses which are related to retaining wall recently increase. However, since the study on measuring and monitoring the pre-stressing force of anchor is insufficient, behavior of anchor may not be predicted and monitored appropriately by the existing strain gauge and load cell type monitoring system. FBG Sensor, which is smaller than strain gauge and has better durability and does not have a noise from electromagnetic waves, is adapted to measure the strain and pre-stressing force of 7-wire strand, so called smart tendon. A series of pullout tests were performed to verify the feasibility of smart tendon and find out the load transfer mechanism around the steel wire tendon fixed to rock with grout. Distribution of measured strains and estimated shear stresses are compared with those predicted by theoretical solutions. It was found that developed smart tendon can be used effectively for measuring strain of 7-wire strand anchor and theoretical solutions underestimate the magnitude of shear stress and load transfer depth.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.40 no.6
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• pp.547-553
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• 2020

As aging infrastructures increase along with time, the efficient maintenance becomes more significant and accurate responses from the sensors are pre-requisite. Among various responses, strain is commonly used to detect damage such as crack and fatigue. Optical fiber sensor is one of the promising sensing techniques to measure strains with high-durability, immunity for electrical noise, long transmission distance. Fiber Bragg Grating (FBG) is a point sensor to measure the strain based on reflected signals from the grating, while Brillouin Optic Correlation Domain Analysis (BOCDA) is a distributed sensor to measure the strain along with the optical fiber based on scattering signals. Although the FBG provides the signal with high accuracy and reproducibility, the number of sensing points is limited. On the other hand, the BOCDA can measure a quasi-continuous strain along with the optical fiber. However, the measured signals from BOCDA have low accuracy and reproducibility. This paper proposed a multi-fidelity data-fusion method based on Gaussian Process Regression to improve the fidelity of the strain distribution by fusing the advantages of both systems. The proposed method was evaluated by laboratory test. The result shows that the proposed method is promising to improve the fidelity of the strain.