• Journal of Microbiology
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• 제35권4호
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• pp.334-340
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• 1997

A collection of 132 temperature sensitive (ts) mutants was generated by the chemical mutagenesis of Schizosaccharomyces pombe wild type strain and screened for tRNA splicing defects on Northern blots by hybridization with an oligonucleotide that recognizes the exon of the S. pombe tRNA^Val as a probe. We identidied 6 mutants which accumulate precursor $tRNA^{Val}$. Among them, 2 mutants exhibited remarkable morphological differences compared to wild type cells. One tRNA splicing mutant showed elongated cell shape in permissive as well as non-permissive cultures. The other mutant exhibited shortened cell morphology only in nonpermissive culture. The total RNA pattern in the splicing mutants appeared to be normal. Genetic analysis of four $tRNA^{Val}$ splicing mutants demonstrated that the mutation reside in different genes.

• Structural Engineering and Mechanics
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• 제52권2호
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• pp.323-349
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• 2014

In this paper, linear buckling analysis of a curved sandwich beam with a flexible core is investigated. Derivation of equations for face sheets is accomplished via the classical theory of curved beam, whereas for the flexible core, the elasticity equations in polar coordinates are implemented. Employing the von-Karman type geometrical non-linearity in strain-displacement relations, nonlinear governing equations are resulted. Linear pre-buckling analysis is performed neglecting the rotation effects in pre-buckling state. Stability equations are concluded based on the adjacent equilibrium criterion. Considering the movable simply supported type of boundary conditions, suitable trigonometric solutions are adopted which satisfy the assumed edge conditions. The critical uniform load of the beam is obtained as a closed-form expression. Numerical results cover the effects of various parameters on the critical buckling load of the curved beam. It is shown that, face thickness, core thickness, core module, fiber angle of faces, stacking sequence of faces and openin angle of the beam all affect greatly on the buckling pressure of the beam and its buckled shape.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 춘계학술대회 논문집
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• pp.54-58
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• 2001

In recent years, hydroforming technology has been one of the most important technology in automotive industry in the points of weight saving, cost reduction and qualify improvement. However, compared with traditional metal forming technology, hydroforming has much fewer information in experience and empirical knowledge. But we don't have my sufficient time and money to produce hydroforming products using real blank directly Therefore Simulation is essential in hydrofonrung technology development. In this paper, we simulate the side member as the tubular hydroforming technology. The manufacturing process of side member' consists of pre_bending, pre_forming, and hydroforming of a thin tube. Variables such as internal pressure, end feeding, and tool geometry are optimized to improve the forming safety. And we simulate side member according to several lubricant conditions. from those simulations, we find that strain distributions can be reduced well by internal pressure and end feeding control, and lubrication is the most important thing in hydroforming process.

• 대한기계학회논문집A
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• 제25권4호
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• pp.619-628
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• 2001

A viscoelastic constitutive equation of rubber is proposed under small oscillatory load superimposed on large static deformation. The proposed model is derived through linearization of Simos nonlinear viscoelastic constitutive model and reference configuration transformation. Statically pre-deformed state is used as reference configuration. The model is extended to a generalized viscoelastic constitutive equation including widely-used Mormans model. Static deformation correction factor is introduced to consider the influence of pre-strain on the relaxation function. The model is tested for dynamic behavior of rubbers with different carbon black fractions. It is shown that the constitutive equation with static deformation correction factor agrees well with test results.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2000년도 봄 학술발표회 논문집
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• pp.751-756
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• 2000

The structural behavior of reinforced concrete slabs strengthened by glass fiber reinforced plastic-panels experimentally investigated. The experimental variables are strengthening length, strengthening width, and pre-crack existence. The pre-cracked slabs are initially loaded to 70 percent of ultimate flexural capacity and subsequently repaired with GFRP-Panels bonded to the tension face of the slabs. Five one-way slabs were tested to failure. The main failure mode of strengthened slabs is separation failure by crack propagation from load point section to end of plate. The behavior of strengthened slabs is represented by a maximum load, load-deflection curves an load-strain curves.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2003년도 가을 학술발표회 논문집
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• pp.577-584
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• 2003

Tension tests of half-thickness concrete containment wall elements and material tests were conducted to derive a crack pattern and constitutive law of concrete. Main test variables are reinforcement ratio and the applied load ratio in two direction, and its effect on the behavior of reinforced concrete panel subjected to biaxial tension is investigated. Based on the test results, analytical expression is derived for the stress-strain relationship of concrete in tension. Ultimate analyses of reinforced concrete panels are carried out by a general purpose structural analysis computer program(ABAQUS), and its results are compared with the test results. The present analysis focuses on the effects of pre-analysis prior to test of specimens. These ultimate tensile analyses as pre-analysis are essential and important to design an effectual scheme of test.

• Advances in nano research
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• 제10권1호
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• pp.1-14
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• 2021

In the present computational approach, thermal buckling and frequency characteristics of a doubly curved laminated nanopanel with the aid of Two-Dimensional Generalized Differential Quadrature Method (2D-GDQM) and Nonlocal Strain Gradient Theory (NSGT) are investigated. Additionally, the temperature changes along the thickness direction nonlinearly. The novelty of the current study is in considering the effects of laminated composite and thermal in addition of size effect on frequency, thermal buckling, and dynamic deflections of the laminated nanopanel. The acquired numerical and analytical results are compared by each other to validate the results. The results demonstrate that some geometrical and physical parameters, have noticeable effects on the frequency and pre-thermal buckling behavior of the doubly curved open cylindrical laminated nanopanel. The favorable suggestion of this survey is that for designing the laminated nano-sized structure should pay special attention to size-dependent parameters because nonlocal and length scale parameters have an important role in the static and dynamic behaviors of the laminated nanopanel.

• 한국식품저장유통학회지
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• 제30권5호
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• pp.785-796
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• 2023

The aim of this study was to elucidate chemical structures and rheological properties of arabinogalactans (AGs) isolated from three legumes including black gram (BG), great northern bean (GNB), and California small white bean (CSWB). The ratio of galactose to arabinose (G/A) in three legumes increased in the order of BG > GNB > CSWB. The rheological measurements of 1-5% (w/v) AG solutions revealed Newtonian and non-Newtonian flow behaviors. BG exhibited yield stress, indicating plastic behavior. Small-amplitude oscillatory tests indicated viscoelastic properties of BG, GNB, and CSWB ranging from solid-like, paste-like, and liquid-like behaviors, respectively. Small-strain oscillatory tests were conducted to assess the structure recovery of the AGs after pre-shearing. G" values of BG and GNB increased, but those of CSWB remained constant after shearing. These results suggest that the chemical structures of the AGs, particularly their G/A ratios, influence their rheological properties.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.776-783
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• 2000

Various alcohol yeast strains have been isolated from main mashes of Korean traditional liquors, and their genetic diversities were previously reported [23]. In this study, the strain SHY111, showing the highest alcohol production, was tested for its fermentation and sporulation characteristics. Additionally, its haploid cells were isolated and tested for their growth and fermentation patterns. The strain was identified as Saccharomyces cerevisiae based on its morphological and physiological characteristics. The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA regions of S. cerevisiae SHY111 were found to be identical to those of S. cerevisiae that was obtained from through the yeast genome project. The maximum fermentation ratio obtained by the strain SHY111 (96.7%) was almost the same as that by S. cerevisiae Balyun No. 1 (96.5%) that was a little higher than that by S. cerevisiae KCCM11215(95.8%). The strain was induced for sporulation in a sporulation liquid medium using log phase cells grown in different types of pre-sporulation media, and its haploid cells were obtained by spore dissection using a micromanipulator. The majority of the spores formed a small colony on a YPD agar plate, and the haploid yeast cells derived from the strain SHY111 showed a variety of growth and alcohol fermentation patterns. It was proposed that the fermentation patterns were related to their growth phenotypes in the most haploid strains, but possible not in some strains.

• Composites Research
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• 제25권6호
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• pp.211-216
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• 2012

광섬유센서는 구조물에 매립 또는 부착시 전단응력에 의해 편광이 발생하여 피크가 2개로 갈라질 수 있기 때문에 센서는 보호되고 양 끝단을 고정하는 방법으로 패키징을 하여야 하나, 광섬유의 클래딩 부분을 부착하는 방식으로 고정하면, 변형발생시 광케이블을 구성하는 코어와 클래딩 사이에서 미끄러짐 현상이 발생하기 때문에 이를 방지하기위해 센서의 양 끝단에 고정구를 사용하고 광섬유를 부분탈피하여 부착하여 접점을 만들어 줌으로써 외력에 의해 발생하는 변형을 정확하게 측정이 가능하도록 하였다. 그리고 기존 광섬유격자센서가 자체적으로 압축변형의 측정이 곤란한 점을 개선하기위해 미리 긴장(Pre-Strain)상태를 유지하기 위하여 두 개의 접점사이를 볼트와 너트로 조절하여 프리스트레인 가변이 가능하도록 하여 인장/압축변형 측정을 가능하게 한 광섬유격자센서 패키지를 제작하였다. 이러한 광섬유격자센서패키지를 실제구조물에 적용하여 측정하였으며, 이를 통하여 안전을 감시하는 모니터링시스템에 적용할 수 있도록 하였다.