• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.41-46
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• 1995

Leaf mesophyll protoplasts of Dianthus superbus were cultured in MSP1 liquid medium supplemented with 0.5 mg/L BAP, 2.0 mg/L NAA and 9% mannitol. Protoplast-derived colonies were formed after 3 to 4 weeks of culture in the dark at 27$^{\circ}C$. These colonies were kept under continuous illumination (21.5 $\mu$E. m-2 sec-1) for 2 weeks and finally most of the colonies became green microcalli, about 3 mm in diameter. When green microcalli were transferred to MS solidified medium with 2.0 mg/L 2,4-D, they formed embryogenic calli after 4 week of culture. These calli were then transferred onto $N_{6}$ medium containing 0.1mg/L 2,4-D, 0.1 mg/L NAA, 2.0 mg/L kinetin and 2.0 g/L casein hydrolysate and cultured under illumination. After 5 weeks of culture the calli gave rise to multiple shoots of 10 to 15 per callus. Upon transfer onto MS medium containing 2.0 mg/L NAA, they were noted. The regenerates were successfully transplanted into potting soil.

• Journal of the Korean Society of Food Culture
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• v.29 no.6
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• pp.499-510
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• 2014

This study examined the food culture of the Koryo Dynasty during the early 13th century based on the records of wooden tablets and marine relics from the 1st and 2nd ships of Mado wrecked at sea off Taean while sailing for Gaegyeong containing various types of grain paid as taxes and tributes. The recipients of the cargo on the 1st ship of Mado were bureaucrats living in Gaegyeong during the period of the military regime of the Koryo Dynasty, and the place of embarkation was the inlet around Haenam (Juksan Prefecture) and Naju (Hoijin Prefecture) in Jolla-do. On wooden tablets were recorded 37 items of rice, cereal, and fermented foods. The measures used in the records were seok [石-20 du (斗)] for cereal, seok [15 du, 20 du] for fermented soybean paste, and pot (缸) and volume (斗) for salted fish. The places of embarkation on the 2nd ship of Mado were Jeongeup (Gobu Prefecture), Gochang (Jangsa Prefecture, Musong Prefecture), etc. On wooden tablets were recorded 29 items of rice, cereal, fermented foods, seasame oil, and honey. The volume measure for yeast guk (麴), the fermentative organism for rice wine, was nang [囊-geun (斤)], and the measure for sesame oil and honey, which were materials of oil-and-honey pastries and confections, was joon (樽-seong, 盛). Honey and sesame oil were luxury foods for the upper-class people of the Koryo Dynasty, and they were carried in high-quality inlaid celadon vases in Meibyung style. Food names and measures written on wooden tablets and actual artifacts found in the 1st and 2nd ships of Mado are valuable materials for research into agriculture, cereal, and fermented foods of the Koryo Dynasty in the early 13th century. Besides, relics such as grains and bones of fish and animals from the Koryo Dynasty are expected to provide crucial information usable in studies on food history of the Korean Peninsula.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.515-516
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• 2006

This experiment was carried out to find out proper culture soil for the flowerpot cultivation of Sedum kamtschaticum. Peatmoss was used for the culture soil. pH of the culture soils were adjusted to 4.5, 5.0, 5.5 and 6.0 using calcium hydroxide. Young plants of Sedum kamtschaticum were planted in the pots of 10cm in diameter. The experiment was conducted by the completely randomized design with 3 replications. Growth characteristics were investigated at intervals of 30 days after planting. As the pH of culture soil is lower, growth of Sedum kamtschaticum showed longer plant height and more number of leaves and branches. pH 4.5 to 5.0 appeared to be optimum range as soil reaction of Sedum kamtschaticum cultivation.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.323-330
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• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.21-43
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• 2015

Korean floricultural industry has grown under the full backing of government, as it placed itself as the main export item since 2000s. Despite its high-speed growth after the domestic production scale of trillion won, the floricultural industry lost its pace after 2005, due to the market-opening of agricultural product and global economic depression. Although the national income is growing and the level of civilized living is following, spending on flowers is faltering. As of such circumstances, necessity for analyzing flower consumption trend and behavior has came out, along with the calls for expansion plan of flower consumption, under the basis of result analysis on consumer reports. The result of the research contains the trend/consumption behavior analysis on domestic and foreign flower industry to boost floricultural industry, along with reviews on various studies of the developmental process of domestic and foreign cut-flowers/pot-plants consumption trend. Also this study has reached to the various recognition of people toward flower, through a public survey. Through such results, this study would like to propose the measures for diffusion of flower-consumption culture, achieving the improvement of life-quality of public along. First, efforts on reviewing the customer accessibility is essential to create flower- culture and the consumption. Second, to eliminate the obstacles that prevents flowers from public's daily life is essential. Third is the effective public-relations on flowers. To create consuming culture and to adhere the positive images, it is essential to research and systematically organize categories of color, scent, and elements of flower and utilize them. Last proposal is the organization of flower-concerned personnels and the leading groups. The change is affected by the economical, social environment, along with the emotions of consumers. Therefore the necessity for the leading group to be the control-tower of such changes are very clear.

• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.62-67
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• 1997

A potential biocontrol bacterium, YB-70 was isolated from a rhizosphere in suppressive soil and identified as a strain of Bacillus subtilis. In several biochemical and in vitro antibiosis tests on Fusarium solani with the culture filterates from B. subtilis YB-70, we found that antifungal mechanism of B. subtilis YB-70 was mediated by antibiotic substances produced from the bacterium. These antifungal substances were appeared to be hear-resistant, micromolecular, and ethy alcohol soluble. Antifungal agents produced by B. subtilis YB-70 showed strong inhibified against root-rotting fungi F. solani in in vivo pot test. An antifungal substance. YBS-1s, was purified from the culture broth of B. subtilis YB-70 by isoelectronic precipitation, silica gel column chromatography and Sephadex LH-20 column chromatography analysis by Fab-MASS, $^{1}$H-NMR, $^{13}$C-NMR, DEPT, and amino acid analyzer revealed that the YBS-1A was a peptide antibiotics of iturin class containing seven amino acids from five different groups, and the other(YBS-1B) was an analogue of iturin group composed of 11 amino acids with larher molecular weight of about 1, 500 dalton, which was lager than that of iturin A.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.298-303
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• 1995

The biological control effect of Trichoderma harzianum on the Fusarium wilt of strawberry and several factors affecting on its efficacy were examined through pot experiments. T. harzianum grown on wheat barn, rice straw, rice hull, sawdust or barley straw was respectively incorporated into the pathogen-infected soil, and significantly suppressed the strawberry wilt caused by Fusarium oxysporum f. sp. fragariae. The wheat bran or rice straw culture of T. harzianum suppressed the disease incidence more effectively than other substrates for culture, decreasing it to 68% of the untreated control. The conidial suspension of T. harzianum alone or the suspension mixed with crab shell also effectively reduced the disease incidence. The control effectiveness of T. harzianum was high in acid soil (pH 3.5~5.5). In sandy loam soil, the disease incidences and population densities of the pathogen were decreased by the treatment of T. harzianum, while there was no significant effect of T. harzianum on the pathogen in loam soil.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1511-1516
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• 2018

In this study, we investigated Indole-3-acetic acid (IAA) production by a rice phylloplane bacteria, Enterobacter sp. DMKU-RP206, using sweet whey as a feed stock instead of lactose. We succeeded in using sweet whey for Enterobacter sp. DMKU-RP206 to produce 3,963.0 mg IAA/l with the optimal medium containing 1.48% sweet whey, 1.42% yeast extract and 0.88% $\text\tiny{L}$-tryptophan. The medium pH was adjusted to 6 and the culture conditions were shaking at 200 rpm on an orbital shaker at $30^{\circ}C$ for 3 days. We also evaluated the effect of IAA in culture filtrates of Enterobacter sp. DMKU-RP206 on the promotion of jasmine rice growth in a pot experiment. Compared with the negative control (without IAA), the result showed that biosynthetic IAA produced by Enterobacter sp. DMKU-RP206 significantly increased the growth of jasmine rice (Oryza sativa L. cv. KDML105) in terms of length and dry weight of shoot. This work thus reveals the impact of IAA produced by Enterobacter sp. on the promotion of jasmine rice growth.

• Korean Journal of Agricultural Science
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• v.37 no.3
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• pp.377-382
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• 2010

An auxin-producing bacterium (AMT-54) was isolated from ginseng cultivating soil of Geumsan area. The isolate AMT-54 was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC analysis. When the concentration of IAA was assessed by performing HPLC quantitative analysis, the maximal 457ppm of IAA was detected from the culture filtrate after culturing in R2A broth containing 0.1% tryptophan for 24h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. The strain AMT-54 was identified as a novel species belongs to Klebsiella mobilis by a chemotaxanomic and phylogenetic analysis. To investigate the growth promoting effect of crop, when the culture broth of K. mobilis AMT-54 was infected onto seed pot of mung bean, the adventitious root induction and root growth of mung bean were 3.3times higher than control.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.2
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• pp.187-193
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• 1990

Ganoderma lucidum has been widely used not only as ingredients in herbal medicine but also in pharmacological soft drinks. The author collected for analysis of content of 8 kinds of heavy metal(Cd, pb, Hg, Cu, Mn, Fe, As) in soil and cluture soil in an around the Taegu area including Sang Ju, Non Gong, Keum Ho, and Weol Bae. THe toxic content in Gaoderma lucidum showed relatively low level as in cadmium lead mercury arsenic : 0.8-0.13ppm 0.17-1.43ppm 0.02-0.32ppm 0.01-0.19ppm respectively : in copper mangenese zinc and iron : 0.93-4.29ppm, 0.37-2.18ppm 1.02-1.65ppm, 4.57-11.04ppm those grown in soil showed higher percentages of content than those grown on logs in lead copper zinc and iron by 43.2% 68.6%, 20.3% and 43.2% respectively. The content of heavy metals in those grown in soil and culture soil tended to be higher in the areas near factories of industrial complexes especially in manganese and iron. The content of heavy metals in soil and culture soil appeared lower than the mean values of Korean Soil. No interrelationship was found in the content of heavy metals between those of Ganoderma lucidum grown on logs and those grown in soil. In case of pot cultivation however the mushroom spawns are grown originally in soil which seems to influence the degree of content of heavy metals of media.