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http://dx.doi.org/10.7744/cnujas.2010.37.3.377

Isolation and Characterization of the Auxin producing Plant Growth Promoting Rhizobacterium from Soil in a Ginseng Field  

Bak, Hae-Sung (Dept. of Bio-Environmental Chemistry, Chungnam National University)
Jung, Young-Pil (Dept. of Bio-Environmental Chemistry, Chungnam National University)
Yoon, Min-Ho (Dept. of Bio-Environmental Chemistry, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.37, no.3, 2010 , pp. 377-382 More about this Journal
Abstract
An auxin-producing bacterium (AMT-54) was isolated from ginseng cultivating soil of Geumsan area. The isolate AMT-54 was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC analysis. When the concentration of IAA was assessed by performing HPLC quantitative analysis, the maximal 457ppm of IAA was detected from the culture filtrate after culturing in R2A broth containing 0.1% tryptophan for 24h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. The strain AMT-54 was identified as a novel species belongs to Klebsiella mobilis by a chemotaxanomic and phylogenetic analysis. To investigate the growth promoting effect of crop, when the culture broth of K. mobilis AMT-54 was infected onto seed pot of mung bean, the adventitious root induction and root growth of mung bean were 3.3times higher than control.
Keywords
Auxin; Klebsiella mobilis; Plant growth promoting rhizobacteria;
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